Labshake search
Citations for Thermo Fisher :
701 - 750 of 8852 citations for M CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
-
bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...
-
bioRxiv - Genetics 2020Quote: ... pAc5.1/V5-His version C (V411020, Thermo Fisher Scientific). In order to test the transferring ability of gypsy ...
-
bioRxiv - Developmental Biology 2021Quote: ... and inserted into pMT/V5-His A (Thermo Scientific) using XhoI and SpeI target sites ...
-
bioRxiv - Cell Biology 2022Quote: pAc5.1/V5-His vector (Thermo Fisher Scientific, Cat #V411020) was used to construct plasmids for expressing proteins or miRNAs in Drosophila S2 cells ...
-
bioRxiv - Molecular Biology 2019Quote: ... and Ion PGM Hi-Q Sequencing Kit (Life Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... with 10% heat-inactivated FBS (HI-FBS; Life Technologies)) ...
-
bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
-
bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
-
bioRxiv - Neuroscience 2020Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
-
bioRxiv - Biochemistry 2020Quote: ... Mouse anti-Penta-His-tag (#P-21315, ThermoFisher Scientific), 1:2000 ...
-
bioRxiv - Microbiology 2020Quote: ... and 9.9 μl of Hi-Di formamide (Applied Biosystems). These were then incubated for 5 min at 95°C and placed immediately on ice ...
-
bioRxiv - Microbiology 2021Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or SARS-CoV-2 N-His (Sino Biological # 40588-V08B ...
-
bioRxiv - Microbiology 2022Quote: ... or the mouse-anti-His (HIS.H8) primary antibody (Invitrogen) (1:1,000 ...
-
bioRxiv - Immunology 2020Quote: ... and ligated into pcDNA3.1D/V5-His-TOPO vector (Invitrogen). Pseudoviruses were prepared by co-transfecting 1.25 µg of HIV-1 envelope containing plasmid with 2.5 µg of an envelope deficient HIV-1 backbone (PSG3Δenv ...
-
bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
-
bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
-
bioRxiv - Molecular Biology 2022Quote: ... Pre-washed 25 µl His-Tag Dynabeads (ThermoFisher Scientific) were added ...
-
bioRxiv - Neuroscience 2019Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
-
bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
-
bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
-
bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
-
bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...
-
bioRxiv - Biochemistry 2021Quote: ... Hi-5 cells (BTI-TN-5B1-4) (Gibco #B85502) were cultured in Express Five™ SFM (Serum-Free Media ...
-
bioRxiv - Immunology 2021Quote: 100 µL of Invitrogen His-Tag Dynabeads (ThermoFisher 10104D) were used for prefusion S2 depletion ...
-
bioRxiv - Developmental Biology 2020Quote: ... and cloned into the pAc5.1-V5/His vector (Invitrogen). Immunoprecipitations between Bdbt-EGFP and Myc-DAnkrd49 used GFP rabbit serum (ab290 ...
-
bioRxiv - Microbiology 2020Quote: ... PCR products were cloned into pcDNA-V5/His (Invitrogen) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The pcDNA4-TO-myc/his B backbone vector (Invitrogen) was chosen for overexpression ...
-
bioRxiv - Molecular Biology 2022Quote: ... 15 mL 6x His-Tag Monoclonal antibody (MA121315; Invitrogen) diluted 1:1500 in blocking solution supplemented with 0.1% NaN3 was added to the membrane and incubated at 4°C on an orbital shaker at 55 rpm for 14-16 h ...
-
bioRxiv - Immunology 2022Quote: ... mouse anti-His antibody (Invitrogen cat. # MA1-21315-1MG) was used at the same concentration to coat the plates ...
-
bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
-
bioRxiv - Microbiology 2023Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or HCoV-OC43 N-His (Sino Biological # 40643-V07E ...
-
bioRxiv - Cell Biology 2022Quote: ... were cloned in pcDNA3.1(-) Myc/His vector (ThermoFisher Scientific). Ligated plasmids were transformed in chemically competent E ...
-
bioRxiv - Cell Biology 2023Quote: ... and into pcDNA™6/myc-His A (Invitrogen), from an amplicon produced by RT-PCR from S2Xpress RNA ...
-
bioRxiv - Biochemistry 2023Quote: ... His-Tag Dynabeads (Invitrogen 10103D, 10 µL per sample) was washed three times with B buffer before protein immobilization ...
-
bioRxiv - Biophysics 2023Quote: His-Tag Dynabeads (Invitrogen 10103D, 10 μL per sample) were manually washed three times with B buffer and resuspended in B buffer (100 μL per sample) ...
-
bioRxiv - Plant Biology 2023Quote: ... Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific) were washed twice in Strep-to-His Buffer before adding the eluted proteins and incubating while rotating for 20 min ...
-
bioRxiv - Microbiology 2023Quote: ... araC and the araBAD promoter from pBAD/His (Invitrogen), the tetracycline resistance gene from pACE2 (Geneva Biotech ...
-
bioRxiv - Biochemistry 2024Quote: Supernatant was supplemented with His-Pur cobalt resin (ThermoFisher), imidazole to 10 mM ...
-
bioRxiv - Immunology 2024Quote: ... 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) were coupled with 200 µg of histidine-tagged SARS-CoV-2 S protein ...
-
bioRxiv - Cell Biology 2020Quote: ... HEK293 Flp-In T-rex cells were maintained in Dulbecco’s modified Eagle’s medium (Invitrogen) containing 5% fetal bovine serum (FBS ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ΔSMO HEK293 cells were transiently transfected in suspension using Lipofectamine 2000 (Thermo Fisher Scientific). 4·105 cells ml−1 were transfected with 50-500 ng of receptor plasmid DNA and 500-950 ng of pcDNA ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNAs were extracted from HEK293 cells or DRG tissues using TRIzol (ThermoFisher Scientific). One μg RNA was used for reverse transcription with iScript Reverse Transcription Supermix (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2021Quote: HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Gemini Bio-products ...
-
bioRxiv - Cell Biology 2021Quote: Culturing of HEK293 and COS-7 cells and their transfection using TurboFect (ThermoFisher Scientific) as well as their immunolabeling was essentially done as described (Kessels et al. ...
-
bioRxiv - Cell Biology 2022Quote: HEK293 cells expressing the tetracycline (Tet)-regulated transactivator were transfected using Lipofectamine 3000 (Invitrogen) with doxycycline (DOX ...
-
bioRxiv - Immunology 2020Quote: ... MCF-7 and HEK293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). Culture media were supplemented with 10% fetal bovine serum ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were grown on Nunc Lab-Tek II Chamber Slide systems (ThermoFisher 154534PK). Twenty four hours post-passage ...
-
bioRxiv - Microbiology 2021Quote: HeLa cells or HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen), containing 10% FBS (#10270 ...
-
bioRxiv - Microbiology 2022Quote: HeLa cells and HEK293 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 10% fetal bovine (Gibco ...