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Citations for Thermo Fisher :
701 - 750 of 8327 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Cells were rinsed four times with PBS and once with distilled H2O before inclusion in ProLong Gold antifade reagent (Life Technologies). Images were taken with a Zeiss LSM700 confocal laser scanning microscope (Carl Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were diluted at 1/10 in H2O and mixed with 250 nM of primers and PowerUp SYBR Green Master Mix (Applied Biosystems) for qPCR ...
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bioRxiv - Genomics 2024Quote: ... We diluted the products 1:4 with MilliQ-H2O and submitted them for Sanger sequencing through the OHSU Vollum DNA Sequencing Core using an ABI (Applied Biosystems) 3730xl 96-capillary DNA Analyzer ...
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bioRxiv - Biochemistry 2024Quote: Lyophilized single strand DNA oligos were obtained from Integrated DNA Technologies and resuspended in miliQ H2O to a concentration on 1 mM measured by nanodrop (Thermo Scientific). Double-stranded DNA was produced by mixing equal amounts of top and bottom strands ...
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bioRxiv - Molecular Biology 2020Quote: ... pyruvate DMEM (4.5 g/L D-glucose, 110 mg/L sodium pyruvate; Gibco™ Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and L-Arginine HCl/L-Lysine-2-HCl (Thermo Fisher 89989 and 89987) or 13C615N4 L-Arginine HCl/13C6 15N2 L-Lysine-2HCl (Thermo Fisher 89990 and 88209) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-alanyl-L-glutamine dipeptide (GlutaMAX™, Gibco, Catalog#: 35050-061) and 0.05 mM 2-Mercaptoethanol (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5 mM L-Glutamine and 12.5 μM L-Glutamate (all Thermo Fisher Scientific) at 85,000 cells/ml on gold grids for cryo-ET experiments ...
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bioRxiv - Biophysics 2020Quote: ... 100 μl Opti-MEM ([+] HEPES, [+] 2.4 g/L Sodium Bicarbonate, [+] L-Glutamine, Gibco), and 1 μl Lipofectamine LTX Reagent (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 2 mM L-alanyl-L-glutamine (Biochrom) and 50 μg/ml Gentamicin (Gibco). The snake cells were maintained at 30°C and mammalian cells at 30°C or 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... GlutaMAX (2 mM L-alanyl-L-glutamine dipeptide - Gibco, Grand Island, New York), 100 units/ml penicillin and 100 µg/ml streptomycin ...
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bioRxiv - Cell Biology 2024Quote: ... Germany) and Leibovitz’ L-15 medium (L-15) from Fisher Scientific (Nidderau, Germany). Foetal calf serum (FCS ...
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bioRxiv - Genetics 2024Quote: ... supplemented with 10% FBS and 1X phytohemagluttinin-L (PHA-L, Thermo Fisher Scientific). The cells were incubated at 37 °C and 5% CO2 for 20 h before being used for nucleofection.
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bioRxiv - Physiology 2022Quote: ... C2C12 cells were differentiated into myotubes with low-glucose DMEM (1 g/L glucose with 4mM L-glutamine and 110 mg/L sodium pyruvate; Gibco 11885-084) supplemented with 2% horse serum (defined ...
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bioRxiv - Microbiology 2023Quote: ... followed by flushing the solution with 20 μL of DPBS (KCl 0.2 g/L, KH2PO4 0.2 g/L, NaCl 8 g, Na2HPO4 1.15 g/L, pH = 7.0–7.3, Thermo Fisher Scientific, Waltham, MA, United States) (Figure S2A Step 3) ...
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bioRxiv - Developmental Biology 2024Quote: ... The remaining proximal part was then transferred either to a 96-well plate with 200 µL of media or an air-liquid interface (ALI) chamber with 2 mL of L-15 media (L-15 (ThermoFisher Scientific, 21083027), 1% Antibiotic-Antimycotic ...
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bioRxiv - Genomics 2020Quote: ... We extracted DNA with 0.9 mL Extraction Buffer (1% SDS [Fisher Scientific], 240 mg/L para-aminosalicyclic acid [ACROS], 484 mg/L Tris/HCl [Fisher Scientific], 365 mg/L NaCl [Fisher Scientific], and 380 mg/L EGTA [MP] at pH 8.5) and 0.9 mL phenol/chloroform (Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... RPMI-1640 media containing 10% FBS was supplemented with antibiotics (6 mg/l Nitrofurantoin, Sigma Aldrich, cat. no. N7878; 16 mg/l Imipenem monohydrate, Sigma Aldrich, cat. no. I0160; 200 mg/l; Gentamicin, Fisher Scientific, cat ...
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bioRxiv - Plant Biology 2022Quote: ... A single Agrobacterium colony containing one of the cloned pEAQ plasmids was inoculated into 5 ml yeast extract broth (YEB) medium (5 g/L tryptone, 1 g/L yeast extract, 2.5 g/L luria broth (Fisher scientific, Waltham, MA, USA), 5 g/L sucrose,0.49 g/L MgSO4·7H2O ...
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bioRxiv - Genomics 2021Quote: ... CCD-18co cells were grown in DMEM with L-glutamine (300 mg/L, Gibco), 25 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... L-Glutamine and sodium pyruvate (110mg/L)) supplemented with 10% fetal bovine serum (Gibco). For adipocyte differentiation ...
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bioRxiv - Molecular Biology 2021Quote: ... for “heavy” 3T3-zDHHC3 cells or normal L-lysine and L-arginine (Thermo Scientific) for “light” 3T3-GFP cells ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were expanded in medium supplemented with 4 mM L-alanyl-L-glutamine (Gibco) in 125 mL and 1 L Erlenmeyer flasks (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mM L-alanyl-L-glutamine dipeptide (GlutaMAX™, Gibco, Catalog#: 35050-061) at 37 °C in a 5% CO2 humidity controlled environment ...
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bioRxiv - Immunology 2020Quote: ... culture media was removed and Leibovitz’s L-15 (L-15) media (Invitrogen, Burlington, ON) supplemented with 3% FCS ...
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bioRxiv - Biochemistry 2020Quote: ... to DMEM deficient in both L-lysine and L-arginine for SILAC (ThermoFisher, A33822). The Hela cell was cultured for eight passages in heavy SILAC medium to reach a nearly complete labeling (>99% ...
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bioRxiv - Genomics 2021Quote: ... were grown in DMEM medium (4.5 g/l glucose, 6 mM L-glutamine, Gibco), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 900 µL L-15/FBS medium (10% FBS in Leibovitz’s L-15 medium (Gibco), 0.340 ± 0.005 Osmolarity adjusted by sucrose ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were starved of L-methionine and treated with L-Azidohomoalanine (AHA) (ThermoFisher, C10102) for 6 h to label nascent proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were grown in neurobasal media free of L-Arginine and L-Lysine (Invitrogen) supplied with either Lysine 8 (U-13C6 ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 g/L (34 mM) L-glutamine (Gibco, 200mM, cat. no. 25030-024) – hereafter called “imaging medium” ...
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bioRxiv - Genetics 2023Quote: ... LCLs were cultured in RPMI 1640 with 300mg/L L-glutamine (Life Technologies, 11875085) supplemented with 20% HyClone FBS (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: SILAC RPMI without L-Arginine and L-Lysine was supplemented with dialysed FBS (Gibco), 2 mM L-Glutamine ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10 mmol/L KCl and 20 mmol/L glucose (both from Thermo Fisher Scientific), and 10 mmol/L MgSO4 and 10 mmol/L MgCl2 (both from Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... The resulting cell suspension was suspended in Leibovitz’s L-15 Medium (L-15, Gibco), supplemented with penicillin (100 U/ml ...
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bioRxiv - Microbiology 2020Quote: ... Samples were ethanol precipitated with 20 μg of glycogen and resuspended in 10 μl of RNase free H2O for chemical fragmentation (Ambion Fragmentation Reagent) at 65°C for 4 min ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were washed 3 times with PBS followed by 3 washes with distilled H2O and were mounted using Prolong (tm) Gold antifade mountant with Dapi (Invitrogen, Cambridge, UK). The slides were left to set for 24 hours in the dark at 4°C before visualization ...
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bioRxiv - Cell Biology 2020Quote: ... A gradient consisting of 0% to 40% B in A (A: 0.1% formic acid [Fisher Scientific, A117], 6% acetonitrile [Fisher Scientific, A955], in H2O [Fisher Scientific, W6] ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed three times with PBST and once with distilled H2O before being mounting on coverslips by using ProLong diamond antifade reagent (Thermo Fisher Scientific) and imaging ...
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bioRxiv - Microbiology 2021Quote: ... After two PBS washes and one final H2O wash the coverslip were sealed with 3µl of Prolong Diamond Antifade reagent (Fisher Scientific Ref P36965).
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bioRxiv - Microbiology 2020Quote: ... end repair was carried out by resuspending beads in 50 μl FastAP mix (39 μl H2O, 5 μl 10x FastAP buffer (Thermo Fisher Scientific), 1 μl Murine RNase Inhibitor (NEB) ...
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bioRxiv - Physiology 2020Quote: ... 10-20 mg of powdered liver was sonicated in 500 μL DEPC H2O and dsDNA determined by fluorometric assay (Cat. # P7589, Invitrogen, Waltham, MA). Whole liver and hypothalamic mRNA were isolated from powered liver samples with TRI Reagent® (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... The RNA concentration in RNAse free H2O was analyzed by measuring the absorption at 260 / 280 nm with a NanoDrop (Thermo Scientific/Peqlab). Additionally ...
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bioRxiv - Neuroscience 2023Quote: ... made up in di H2O to stain for nuclei before being mounted or cover-slipped with ProLong Gold mounting medium with DAPI (Fisher Scientific, MA) on slides for imaging with a Zeiss confocal microscope (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... The eluted ligation products were collected and dilute with 20 ul H2O as qPCR template and qPCR was performed in 384-well plate (ThermoFisher cat# 4309849). The delta-delta Ct method was used for calculating PLA value in qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... Probe circularization and RCA was initiated by applying a reaction mix in DEPC-H2O containing 1× Phi29 buffer (Cat No. EP0094; Thermo Fisher Scientific), 1 U/μL Phi29 DNA polymerase (Cat No ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted for 1 h at room temperature with 80 µg of TMT10plex (Thermo Scientific, #90111)94 label reagent dissolved in 4 µl of acetonitrile ...
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bioRxiv - Genomics 2022Quote: ... 2.5% dextran sulfate, 0.2% BSA, 100 μg/mL salmon sperm DNA, 2X SSC, 0.1 U/μL SUPERase•In™ [Thermofisher] in DEPC H2O) was then pipetted into the chamber ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides of ER membranes were reconstituted in 10 μl of H2O and reacted for 1 h at room temperature with 80 μg of TMT10plex (Thermo Scientific, #90111) (Werner et al ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted for 1 h at room temperature with TMT16pro labelling reagent (Thermo Scientific, #A44522). To this end ...