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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for 7 MethoxybenzoFuran 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... developing a linear gradient from 2% to 80% acetonitrile in 0.1% formic acid within 60 minutes (RSLC nano, Dionex – Thermo Fisher Scientific) and analyzed by MS/MS ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.2% SDS) at 50°C for 60 min on a Thermomixer (1200 rpm) followed by extraction with acid phenol (ThermoFisher, AM9712) and precipitation with 1:1 isopropanol:ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... using solvent A (0.1% formic acid in water) and separated on a C18 PepMap RSLC column (2 cm, 100 A; Thermo Fisher Scientific) using a linear gradient from 7 to 30% of solvent B (0.1% formic acid in acetonitrile ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% formic acid in acetonitrile) on an EASY-Spray column ES801 (50 μm ID x 15 cm, 2 μm, Thermo Scientific) mounted on a DPV ion source (New Objective ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with 0.5 µM Lipi-Deep Red neutral lipid stain (Dojindo #LD04-10) for 2 hr and 5 µg/mL Hoeschst 33342 nucleic acid stain (Invitrogen #H3570) for 30 min at 37°C ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... each well was gently rinsed once with RPMI and then starved for 50 min in 2 mL of RPMI (without amino acids, glucose or FBS) with 20 μM Lysotracker Red DND-99 (Invitrogen, L7528). After 50 min of starvation ...
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bioRxiv - Microbiology 2020Quote: Dried samples were reconstituted in mobile phase A solvent (2% acetonitrile and 0.1% formic acid) for analysis on the Q-Exactive™ HF-X mass spectrometer (ThermoFisher Scientific), interfaced to the Easy nanoLC1200 HPLC system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Q-Exactive plus Mass Spectrometer (Thermo Fisher Scientific) at 300 nl/min via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... All data were normalized to the total protein content determined in protein pellets after neutralization with 10 N NaOH and sonication in 2% SDS plus 8 mM EDTA using a bicinchoninic acid assay (ThermoFisher Scientific) and bovine serum albumin as a standard.
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bioRxiv - Immunology 2020Quote: ... tryptic peptides obtained from StageTip-based SCX fractionation were reconstituted in 0.1% formic acid and loaded on a nanoViper 2 cm (3 µm C18 Aq) trap column (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (50 cm ...
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bioRxiv - Neuroscience 2022Quote: ... UM was changed for 2 days to an endothelial cell medium supplemented with retinoic acid (EC+RA: human Endothelial-SFM (Life Technologies), 1% platelet-poor plasma derived bovine serum (Alfa Aesar) ...
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bioRxiv - Microbiology 2022Quote: ... Amino acids were extracted on ice-cold lysis buffer [5:3:2 ratio of methanol-acetonitrile-water (Fisher Scientific, Pittsburgh, PA)] containing 3 µM of amino acid standards [Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... The dried peptide extracts obtained were dissolved in 12 μl of 0.05% trifluoroacetic acid in 2% acetonitrile and analyzed by online nanoLC using an Ultimate 3000 RSLCnano LC system (Thermo Scientific Dionex) coupled to an LTQ Orbitrap Velos mass spectrometer (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: A codon-optimized gene encoding for SARS-CoV-2 (331 to 528 amino acids, QIS60558.1) was expressed in Expi293 cells (Thermo Fisher Scientific) with human serum albumin secretion signal sequence and fusion tags (6xHistidine tag ...
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bioRxiv - Plant Biology 2019Quote: ... They were then diluted 10x with loading buffer (2% acetonitrile, 0.05% trifluoroacetic acid) and injected on a Fusion Tribrid orbitrap mass spectrometer (Thermo Fisher Scientific) interfaced to a Dionex RSLC 3000 nano-HPLC ...
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Genomics 2020Quote: ... The resuspended peptides in sample loading buffer (2% acetonitrile and 0.05% trifluoroacetic acid) were separated and analysed on an UltiMate 3000 RSLCnano HPLC system (Thermo Fisher Scientific) coupled online to either a Q Exactive HF or an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Then the pellets were resuspended in 10 mL of sterile water and transferred to a 15 mL falcon tube containing 2 g of acid washed glass beads (0.5 mm of diameter, Thermofisher, G8772-100G). Cells were then disrupted using a Fastprep system (MP Biomedicals) ...
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bioRxiv - Cancer Biology 2022Quote: ... The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific) and the enzyme inhibitors were reconstituted according to manufacturers’ instructions ...
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bioRxiv - Biophysics 2022Quote: ... Electron microscopy grids were prepared in 2% (w/v) phosphotungstic acid (pH 7.5) and were examined in a Talos L120C transmission electron microscope (FEI-Thermo Fisher Scientific). Nanodisc samples were frozen in aliquots and stored at −80°C.
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bioRxiv - Microbiology 2022Quote: ... and cells were blocked in 500 μl of FACS buffer (2% FBS and 0.2 mM ethylenediaminetetraacetic acid (EDTA) in PBS (Gibco, Thermo Fisher Scientific)) ...
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bioRxiv - Molecular Biology 2023Quote: Phosphor imaging of [γ-32P] radio-labelled DNA sequences was accomplished through 6% denaturing PAGE constituted of 1X TBE (89 mM Tris base, 2 mM EDTA and 89 mM boric acid, Fisher Scientific) (57) ...
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bioRxiv - Molecular Biology 2022Quote: ... The dried peptide extracts obtained were dissolved in 17 μl of 0.05% trifluoroacetic acid in 2% acetonitrile and analyzed by online nanoLC using an Ultimate 3000 RSLCnano LC system (Thermo Scientific Dionex) coupled to an LTQ Orbitrap Velos mass spectrometer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Q-Exactive plus Mass Spectrometer (Thermo Fisher Scientific) or a Orbitrap Fusion Lumos equipped with a FAIMS Pro interface at 300 nl/minute via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... The WIV was then dialyzed against 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered saline (Thermo Fisher Scientific, Bleiswijk, the Netherlands) at 4°C overnight to remove β-propiolactone ...
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bioRxiv - Microbiology 2023Quote: ... the cornea containing molten scaffold medium was placed epithelial side down in a 6 well-plate containing 400 µl of Ethylene glycol-O,O’-bis(2-aminoethyl)-N,N,N’,N’-tetraacetic acid (EGTA) (Fisher scientific) and was kept in a CO2 (5% v/v ...
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bioRxiv - Microbiology 2023Quote: C18 purified digests were re-suspended in Buffer A* (2% acetonitrile, 0.01% trifluoroacetic acid) and separated on a Ultimate 3000 UPLC (Thermo Fisher Scientific) with a two-column chromatography set up composed of a PepMap100 C18 20 mm x 75 μm trap and a PepMap C18 500 mm x 75 μm analytical column (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Eclipse Mass Spectrometer (Thermo Fisher Scientific) or a Orbitrap Fusion Lumos equipped with a FAIMS Pro interface both at 300 nl/minute via the analytical columns using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... and then separated using a 180 min RP gradient (4–30% acetonitrile/0.1% formic acid) on an Acclaim PepMap100 RSLC C18 analytical column (2 μm, 75 μm id x 50 cm, [Thermo Scientific]) with a flow rate of 0.3 μl/min ...
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bioRxiv - Cell Biology 2024Quote: ... iRBCs were washed twice with media to remove extracellular merozoites before being aliquoted in triplicate into a 96-well U-bottomed plate and stained with the nucleic acid dye SYTO-61 (2 μM) (Thermo Fisher) for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and cells were blocked in 500 μl of FACS buffer (2% FBS and 0.2 mM ethylenediaminetetraacetic acid (EDTA) in PBS (Gibco, Thermo Fisher Scientific)) ...
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bioRxiv - Microbiology 2023Quote: Proteome samples were re-suspended in Buffer A* (0.1% trifluoracetic acid, 2% acetonitrile) and separated using an Ultimate 3000 UPLC (Thermo Fisher Scientific) equipped with a two-column chromatography set up composed of a PepMap100 C18 20 mm x 75 μm trap and PepMap C18 500 mm x 75 μm analytical column (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Plant Biology 2024Quote: Dried peptides were suspended in 0.1% formic acid (FA) with 2% ACN and analyzed by LC-MS/MS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) connected to an EASY nLC 1200 (buffer A ...
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bioRxiv - Physiology 2023Quote: The dried polar extracts were prepared for GC-MS analysis through solubilization in 40 µL of 2% methoxyamine hydrochloric acid in pyridine (Fisher Scientific) at 60°C for 60 min and derivatization with 60 µL of N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA ...
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bioRxiv - Pathology 2024Quote: ... the peptide was reconstituted in 2% acetonitrile/0.1% formic acid (FA) and analyzed by an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific) coupled online to an UltiMate 3000 RSLCnano System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were cοtransfected with 7 μg CRISPR plasmid and 7 μg non-linearized HDR template using Lipofectamine 3000 according to the instructions (Thermo Fisher). 48 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... and the cell pellet was resuspended in annexin buffer with Annexin V-FITC and 7-AAD according to the manufacturer’s specifications (FITC Annexin V 7-AAD-Thermo Fisher®). Flow cytometry was performed using Attune flow cytometer (Thermo Fisher®) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with FACS buffer and incubated with the live-dead marker 7-aminoactinomycin D (7-AAD; Thermo Fisher Scientific) for 10 min at 4 °C in the dark ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis induction was determined by activation of Caspase 3 and 7 using the CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen).H460 cells were plated at 5 × 103 cells/well in black 96 well plates with clear bottoms (Costar ...
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bioRxiv - Cancer Biology 2021Quote: ... and mPRs (PAQR5, 6, 7, 8, 9) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems). RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf) ...
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bioRxiv - Evolutionary Biology 2020Quote: ISH and dFISH were performed as previously described (3, 7, 62) with addition of T1 RNAse treatment (2 u/µl, Thermo Fisher Scientific, Waltham, MA, USA) after probe washing in embryos older than 4 dpf to reduce background staining ...
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bioRxiv - Bioengineering 2021Quote: Human lung fibroblasts (hTERT T1015, abmgood) were used between passages 7-12 and culture medium was changed every 2-3 days (Gibco Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly isolated MNCs were washed and re-suspended in PBS and stained for 10 min at 37°C with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) at a concentration of 5 µM (Thermo Fisher Scientific, Waltham, MA, USA). H2DCFDA is a non- fluorescent dye which is cleaved inside cells to 2’,7’-dichlorofluorescein (H2DCF) ...
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bioRxiv - Bioengineering 2021Quote: ... Lauric acid (dodecanoic acid, LA, 99%, Figure S1(c)) was obtained from Acros Organics. The phospholipids DOPC (1,2-dioleyoyl-sn-glycero-3-phosphocholine ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Formic acid (FA) and hydrochloric acid were purchased from Fisher Scientific (Portsmouth, NH, USA). Stock solutions were dissolved in water individually at a final concentration of 1 mg/mL ...