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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for 6 Fluoro 1 3 benzodioxene 8 carbonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Each oligo was diluted to 100 μM in nuclease-free 10 mM Tris pH 8 (diluted from Ambion 1 M Tris pH 8; Invitrogen; cat. no. AM9856). The rRNA depletion mix was generated by combining equal volumes of each 100 μM oligonucleotide stock.
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
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bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
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bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
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bioRxiv - Cell Biology 2023Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole or TO-PRO-3 (Thermo Fisher Scientific) staining.
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
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bioRxiv - Cancer Biology 2021Quote: ... and TO-PRO-3 (1:3000; Thermo Fisher) for fluorescent labelling of living and dead cells (‘Imaging medium’) ...
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bioRxiv - Bioengineering 2019Quote: ... TOPRO-3 (1:1000, in PBS) (Invitrogen, USA) was used for 15 min at room temperature to stain the nuclei.
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bioRxiv - Genetics 2021Quote: ... 8ul of 1:3 Vectashield (Thermo Fisher Scientific) DAPI:PBS was added to samples and allowed to incubate for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... 1× 10−3 М glutamine (Gibco, Cat:25030149) and 2 mg/ml gentamicin (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... in 1:3 ratio using lipofectamine (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... TO-PRO-3 (Thermo Fisher T3605, 1:10,000) to label dead cells ...
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bioRxiv - Neuroscience 2021Quote: ... the DNA dye TOPRO-3 (1:1000, Invitrogen), diluted in a blocking buffer for 2 h at 24 °C ...
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bioRxiv - Cell Biology 2021Quote: ... pan-cytokeratin (ThermoFisher, 3-9003-82, 1/50), pSmad1/5/8 (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... then washed 3 times with 1×PBS (Invitrogen). Roots were then squashed under coverslips onto slides that had been pre-treated with 3-Aminopropylthiethoxysilane (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... 3) donkey anti-rabbit 488 (1:250, Invitrogen). To visualize immunofluorescence ...
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bioRxiv - Neuroscience 2023Quote: ... anti-V5 (Invitrogen, #R96025, 1:800, 3 days), anti-cFOS (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... at pH 8 containing 8 M urea (Thermo Fisher Scientific, USA). To reduce the sample ...
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bioRxiv - Developmental Biology 2023Quote: Essential 8 medium consisted of essential 8 basal medium (Gibco, A1517001) supplemented with 50x essential 8 supplement.
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bioRxiv - Molecular Biology 2021Quote: ... Samples of SMG6-3xFLAG in vitro translation reactions were run on 3-8% Tris-Acetate NuPAGE gel (Invitrogen, Cat. No EA03752BOX). Transfer onto nitrocellulose membranes was performed using the iBlot 2 Dry Blotting System (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10-20 µg of protein loaded on a 3%–8% NuPAGE Tris-Acetate Gel in tris-acetate buffer (Novex Life Technologies) or a 4-12% NuPAGE TrisBis NuPAGE gels in MES buffer (Novex Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... and checked for purity and quality on a silver-stained SDS-PAGE gel (3-8% Tris-Acetate Novex, Thermo Fisher Scientific). The anti-NTD neutralizing antibody NTD-18 and the pan-coronavirus anti-S2 non-neutralizing antibody Ab-10 were previously described 9,10.
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bioRxiv - Cancer Biology 2019Quote: ... LDHA proteins in tumor extract were separated on 4% to 20% Tris-Glycine gel and CD31 was separated on NuPAGE 3 to 8 % Tris-Acetate gel (Life Technologies) by SDS-PAGE and were transferred to nitrocellulose membrane ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg protein/lane was then separated under denaturing conditions on reducing 3~8% Tris-Acetate gels (NuPAGE, Thermo Fisher Scientific), and HiMark protein standard (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were denatured by adding 4xLDS buffer and heating at 70°C for 10 min before being separated in 3-8% Tris-acetate gels (Thermo Fisher) run at 150 V for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then resolved on 4-12% Bis-Tris gels (for lower molecular weight proteins) or 3-8% Tris-Acetate gels (for higher molecular weight proteins) (NuPage, Thermo-Fisher Scientific) and run in MOPS or Tris-Acetate running buffer respectively ...
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were resolved using either home-made SDS-PAGE gels or 3-8% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific), when analysing proteins of a molecular weight >180 kDa ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Biophysics 2022Quote: ... Purified surface proteins or total cell lysates were separated on a NuPage 3-8 % Tris-acetate protein gel (Thermo Fisher Scientific) at 200 V for 40 min and transferred to a PVDF membrane ...
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bioRxiv - Immunology 2022Quote: ... The quality/purity of the different purified fractions was evaluated by SDS-PAGE using 3–8% Tris– Acetate gels (Life Technologies) under non-reducing conditions followed by silver staining (Silver Stain kit ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were passed through a 25G syringe five times to reduce viscosity and separated on a 3%-8% NuPAGE Tris-Acetate gel (Thermo Fisher). Proteins were transferred onto a polyvinylidene fluoride membrane (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Developmental Biology 2021Quote: ... FAPs were plated at a starting density of 3 X 104 cells/cm2 and grown for 8 days in DMEM (Life Technologies) containing 5% FBS plus 1% Pen/Strep ...
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bioRxiv - Developmental Biology 2020Quote: ... For the detection of Gli3 10μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Microbiology 2022Quote: Proteins (8 μM final) were incubated with 0.16 mM of (2'-(or-3')-O-(trinitrophenyl) adenosine triphosphate (TNP-ATP) (Thermo Fisher Scientific), in 50 μl of Binding Buffer (10 mM HEPES-KOH (pH 8) ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Molecular Biology 2022Quote: ... 2-3 mg/mL proteins were applied to glow-discharged M026-Au300-R20/20 Copper grid (CryoMatrix) and blotted for 3 s at 8 ℃ with 100% humidity and plunge-frozen in VitroBot Mark IV (Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Neuroscience 2023Quote: ... To revive hiPSCs from cryopreservation cells were thawed by incubation for 2 minutes at 37°C and retrieved by centrifugation (200 x g for 3 minutes) prior to resuspension in Essential 8 (E8, (A1517001, Life Technologies) medium with 1’RevitaCell ...