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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for 4 1 1 2 3 3 3 Hexafluoropropoxy acetophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL NT-3 (PreproTech) and 1 µg/mL Laminin (Gibco, Thermo Fisher) plus DOX ...
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bioRxiv - Immunology 2024Quote: ... using PEI (3:1 ratio) for transfection of 293F cells and ExpiFectamine (ThermoFisher, #A14525) for transfection of Expi-293F cells according to the manufacturer’s suggestion ...
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bioRxiv - Cell Biology 2024Quote: ... for 3 min and 1:500 of Hoechst 33342 (20 mM, Thermo Fisher Scientific) for 10 min ...
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bioRxiv - Physiology 2023Quote: ... and Cy-3 conjugated anti rabbit IgG at (dilution 1:300) (A10520, Thermo Fisher) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Stable polyclonal populations of cells were selected using 3 μg ml-1 puromycin (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... Cells were transfected with 1 µg total DNA and 3 µL Lipofectamine 2000 (Invitrogen). Media was changed 4 h post-transfection to standard maintenance media and cells were harvested for microscopy or western blot 24 h post-transfection.
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... cerebella were washed 3 × 1 h in 50% OCT (Fisher Scientific, 23-730-571) before embedding in 100% OCT ...
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bioRxiv - Developmental Biology 2023Quote: ... Primed mESCs were passaged every 3 days using 1 U/ml of Dispase (Gibco) as a colony detachment reagent ...
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bioRxiv - Bioengineering 2024Quote: ... Invitrogen) was diluted 1:250 in 3% BSA and 0.1% Tween 20 (Thermo Scientific) in PBS (IF solution) ...
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bioRxiv - Genomics 2024Quote: ... fixed in freshly made fixative [3:1 methanol:acetic acid (Fisher Scientific, Waltham, MA, USA)] ...
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bioRxiv - Neuroscience 2024Quote: ... larvae at 5 dpf were incubated in 3 µM FM 1-43 (Thermofisher, T3163) in E3 media for 35 s ...
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bioRxiv - Immunology 2024Quote: ... unless otherwise specified: αCD40 mAb (Invitrogen or BioLegend, Clone HM40-3, 1 μg/mL), IL-4 (R&D Systems ...
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bioRxiv - Neuroscience 2024Quote: ... mouse monoclonal anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH, 1:5000, Thermofisher, #AM4300, RRID: AB_2536381), overnight ...
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bioRxiv - Microbiology 2024Quote: ... Keratinocytes were passaged in Rheinwald-Green medium (3:1 Ham’s F12 (GIBCO 11765-054) / 4.5 µg/ml glucose Dulbecco’s modified Eagle’s medium (GIBCO 11960-044) ...
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bioRxiv - Cell Biology 2020Quote: ... and then loaded with fluorescent Ca2+ probes (3 μM of fura-2 AM or 5.69 μM of Fluo-4 AM, Life Technologies) in HBSS for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected in zero Ca2+ HL-3 solution at room temperature and stimulated with a HL-3 solution of 90 mM K+/2 mM Ca2+/4 μM FM4-64 (Invitrogen) for 5 min to load FM4-64 dye into the boutons ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were treated with RNAScope H2O2 for 4 min at RT and subsequently washed 2 x 3 min with UltraPure Distilled Water (Invitrogen) at RT ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Developmental Biology 2021Quote: ... were crossed for 2-3 days and then transferred to fly food made from 0.6 g of Carolina Formula 4-24 (Fisher Scientific) and 2 mL of ddH2O ...
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bioRxiv - Genomics 2024Quote: ... and transfer plasmids were transfected at a mass ratio of 2:3:4 with Lipofectamine 3000 (Thermo Fisher Scientific L3000001) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Biophysics 2022Quote: ... N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (NBD-PE) was purchased from ThermoFisher (Waltham, MA). Cholesterol Oxidase from Streptomyces was purchased from MP Biomedicals (Santa Ana ...
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bioRxiv - Cell Biology 2024Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole (DAPI) or TO-PRO-3 (Thermo Fisher Scientific) staining ...
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bioRxiv - Cell Biology 2024Quote: ... or BODIPY 558/568 C12 (C12) (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (ThermoFisher, #D3835) were complexed with fatty acid-free bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 1 mL of the following mixture to the culture media and incubated the cells at 25°C incubator for 1—3 hours: 5 μM Fura-2 AM (F-1201, Life Technologies), 250 μM probenecid (162-26112 ...
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bioRxiv - Biophysics 2021Quote: α2-GFP or α2:β at 1:3 ratio was transfected into HEK293T cells on collagen coated glass bottom dishes using lipofectamine 3000 (Invitrogen) for expression of α2 GlyR or α2β GlyR ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...
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bioRxiv - Biochemistry 2022Quote: ... and then 1 μg of each peptide sample was loaded on a rap column (75 µm × 2 cm, 3 µm, C18, Thermo Fisher). The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... and applied to plasma-cleaned CF-300 2/1 grids before being blotted for 3 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transferred to a new tube ...
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bioRxiv - Molecular Biology 2022Quote: ... The grids were washed by dipping 2 times in PBS (37°C) before 3 μL of 1 μm Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The products were enzymatically cleaned (3 ul of PCR reaction, 2 ul water, 1 ul of ExoSap-IT (Applied Biosystems 78200)) for 15 minutes at 37°C followed by 15 minutes at 80°C ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 μg DNA) and β-arrestin-2-mCherry (1 μg DNA) using 3 μL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM ATP and 1% fraction V BSA) and loaded with 3 μM Mag-Fura-2 AM (Thermo Fisher Scientific, #M1292) at room temperature for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... DAPI was allowed to dry for approximately 3 minutes before immediately coverslipping with 1-2 drops of ProLong Gold (Invitrogen, P36930). Slides were protected from any surrounding light ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed once with PBS and 3 mL of DMEM (2% FCS) containing 1% low melting point agarose (ThermoFisher Scientific) was added to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug dilution series (1:3) were prepared in PDAC organoid culture medium containing 1 μg/ml Hoechst (Invitrogen) and 1 μg/ml PI (Sigma) ...
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bioRxiv - Physiology 2022Quote: ... Plasmid DNA was mixed with PEI (1 mg/ml) at 1:3 in Opti-MEM medium (Gibco, 11058021) and added to cells after short incubation ...
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bioRxiv - Genetics 2022Quote: Calu-3 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM)/F12 (1:1) GlutaMAX™ (ThermoFisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the tumoroids in a 1:3 and 1:10 effector:target (E:T) ratio in RPMI 1640 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... stained with TO-PRO-3 diluted 1:1000 in DPBS and 1 µM Hoechst 33342 (Thermo Fisher 62249) nuclear counterstain for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 3 μg mRNA at a mass ratio of 1:1 using Lipofectamine MessengerMAX (Invitrogen) according to the manufacturer’s guide ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and (MtWOX5) 5’-CACCATGCAGACGGTCCGAGATCTGTC-3’ and 5’- CCTTCGCTTAAGTTTCATGTAA-3’ (AhWOX5) and cloned into pENTR-dTOPO (Invitrogen). The entry clones of AhWOX5 & MtWOX5 recombined through LR clonase Gateway technology (Invitrogen ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... The eight rice 14-3-3 genes cloned in the yeast two-hybrid vector pDEST22 (Invitrogen) were used from a previous study (Deb et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 24 minutes in a mixture of 3% 3-Aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC EtOH ...
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bioRxiv - Immunology 2020Quote: ... 3 μL RT reaction mix consisting of 3 μL of 50 μM random hexamers (Thermo Fisher), 0.8 μL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Immunology 2023Quote: ... To assess active caspase 3 activity the CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit (Invitrogen) was used ...