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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for 3' Methyl 1 1' biphenyl 4 amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Donor embryos were injected at the 1-cell stage with 3-5nL of 1% Dextran-FITC 10,000 MW (Molecular Probes) and 0.05% Phenol Red (to see the solution ...
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bioRxiv - Cancer Biology 2022Quote: ... AF6-88.5.5.3, #17-5958-82), CD119 (1:100, 2E2, #13-1191-82), Armenian Hamster IgG isotype (1:100, eBio299Arm, #13488881) (Invitrogen); CD45 (1:400 ...
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bioRxiv - Developmental Biology 2022Quote: Organoids were treated immediately after PEG embedding for 3 days with neural differentiation medium comprised of a 1:1 mixture of neurobasal medium (GIBCO) and DMEM/F12 (GIBCO) ...
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bioRxiv - Pathology 2020Quote: ... 6-dihydroxy-2,4,5,7-tetraio-dospiro (isobenzofuran-1(3H),9[9H] xanthan)-3:1 dipotassium salt (50 mg/kg in 0.9% saline, Fisher Scientific) was injected retro-orbitally before catalyzing vessel injury with a 540 nm laser ...
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bioRxiv - Molecular Biology 2020Quote: ... To 1/3 of the sample 1/10 of the recommended amount of spike-in (ERCC RNA spike-in mix, Ambion) was added ...
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bioRxiv - Cell Biology 2021Quote: ... the media was replaced entirely with fresh retinal differentiation media (RDM) (DMEM:F12 3:1, 2% B27 supplement, MEM NEAA, 1× antibiotic, antimycotic (Thermo Fisher) and 1× GlutaMAX)) ...
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bioRxiv - Cell Biology 2020Quote: ... the media was changed to retinal differentiation medium (RDM;DMEM:F12 3:1, 2% B27 supplement, MEM NEAA, 1× antibiotic, antimycotic (Thermo Fisher) and 1× GlutaMAX) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL (10μM) reverse primer (ldh4_R, 5’-AATCACAGCAGCCCCTTG-3’) and 1 μL (1 U/μL) Platinum Taq DNA polymerase (Invitrogen, 10966-018) in a 50 μL total reaction volume ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibody in PBS supplemented with 3% BSA (Thermofisher, Mouse 800, 1:20000 and Thermofisher, Rabbit 680, 1:20000) was incubated in the dark for 1 hour on a rolling platform at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Tenfold dilutions were used to infect confluent Vero E6 cells in a 96-well plate in MEM (5% FBS, 1% penicillin-streptomycin, 1% kanamycin, 3% amphotericin B (Gibco)) at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:3) (60) followed by secondary Alexa Fluor 488 goat α-mouse IgG antibody (Invitrogen / Thermo Fisher Scientific – 1:1,000).
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bioRxiv - Neuroscience 2023Quote: ... the brains were placed in the secondary antibody solution containing 1xPBS/0.1% Triton X-100/3% donkey serum and a 1:1000 dilution of donkey anti-sheep 647 (A-21448, Thermofisher) for 10 days.
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bioRxiv - Systems Biology 2023Quote: ... The bottom tips were packed sequentially with three plugs of C18 membrane (3 M Empore) and mixed-mode ion exchange beads (SCX:SAX = 1:1, Applied Biosystems). The quantity of C18 membrane and mixed-mode ion exchange beads was adjusted based on the protein amount.
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bioRxiv - Cancer Biology 2023Quote: ... Buffy coat was mixed 1:2 with PBS and added onto a Ficoll gradient in a 3:1 ratio (Invitrogen). This was centrifuged at 2100 rpm for 25 minutes at RT (w/o brakes) ...
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bioRxiv - Bioengineering 2023Quote: ... was reduced on the 78 ± 3 nm cores using 1 ml of 1 mM L-ascorbic acid (AA) (Fisher Scientific), making core@shell structures ...
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bioRxiv - Physiology 2024Quote: ... WT and Mrp14-/- bone marrow neutrophils (2.5×106 mL-1) were resuspended in PBS and loaded with 3 µM Indo-1 AM (Invitrogen™) for 45min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in 1X PBS (3 x 10 m) before a 1 h incubation in Donkey anti-rabbit IgG (1:1000) (#A32754, Invitrogen) and Goat anti-chicken IgG (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... the PBS or virus dilution in PBS was aspirated and 3 ml of overlay consisting of 1:1 2 x DMEM (DMEM high glucose, no sodium bicarbonate buffer powder [Gibco # 12-100-046] in 500 mL of RNase-free water ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 1:10,000 Hoechst for 1-3 mins and mounted on glass slides with ProLong glass antifade mountant (Invitrogen, P36982).
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4) for 10mins and later incubated with 4 uM fluo-4/AM (1 mM, Molecular probes, #F14201) and 0.002% pluronic F-127 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... and counterstained with 4′,6-diamidin-2-phenylindole (DAPI, 4 μg mL-1, Thermo Fisher Scientific, MA, USA). From the 18 biofilms from each year ...
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bioRxiv - Neuroscience 2024Quote: ... for 4 hr at 4°C followed by 30 min counterstain with Hoechst 33342 (1:5000, Invitrogen, H3570). Following 3 x 10 min washes in PBS ...
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bioRxiv - Microbiology 2020Quote: ... The wells were washed 3 times with 1 mL PBS 1X and covered with a mixture containing secondary goat anti-rabbit-Alexa488 antibodies (Invitrogen Molecular Probe 2mg.mL-1, 1/300e) and DAPI (1/100e ...
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bioRxiv - Neuroscience 2024Quote: ... Medium was changed every 2-3 days and cells were passaged every 3-4 days using Versene (ThermoFisher) and medium supplemented with Y-27623 ROCK inhibitor (Tocris) ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM PMSF) and precleared for 1 hour at 4°C with 25 μl of protein G Dynabeads (Invitrogen). The precleared chromatin solution (1.5 × 106 cells ...
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Cancer Biology 2020Quote: ... new organoids were fixed with 4% paraformaldehyde for 1 day and then incubated with the phalloidin (1:40; Invitrogen) and Hoechst 33342 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Primary staining’s were performed overnight at 4 °C with antibodies to TRF2 and POT-1 (1:300; Abcam and ThermoFisher,); CD3 (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... p21) and pAAV target plasmid in a 4:1:1:2 molar ratio by use of Lipofectamine 2000 (ThermoFisher) according to company protocol ...
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bioRxiv - Genomics 2024Quote: ... was coupled for 4 hours to 40µl of 1:1 mix Protein G and Protein A Dynabeads (Thermo Scientific) and used for overnight chromatin immunoprecipitation in IP buffer (1% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... The relative expression of target cDNAs within the sample set was calculated from a serial dilution (1:4–1:256) (standard curve) of a cDNA pool using StepOneTM software V2.0.2 (Applied Biosystems). Real-time PCR data were then normalized to elongation factor-1 alpha (eF1α ...
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bioRxiv - Bioengineering 2024Quote: ... Plasma samples were thawed from -80°C storage to room temperature and diluted 1:4 with 1× PBS (Gibco). Samples were then transferred to a 96-well clear bottom black plate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged with 80-90% confluence at a split ratio of 1:2-1:4 using TrypLE (Gibco) for 8 min at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... A 1 mL aliquot of 2:2:1 (v/v) mixture of ≥ 99.9% purity acetonitrile (Fisher Scientific; Cat.A998-4) ≥ 99.8% purity methanol (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The following primary antibodies and dilutions were used overnight at 4 °C in PBST with 1% milk: mouse anti- V5 conjugated to horseradish peroxidase (HRP; 1:5,000; Invitrogen), mouse anti-V5 (1:5,000 ...
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bioRxiv - Microbiology 2023Quote: ... 5′-labelled Aar RNA (4 nM) was incubated for 1 h at 37°C with 1× structure buffer (Ambion), 1 µg yeast RNA and putative mRNA interaction partners at the indicated concentrations (0 nM ...
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bioRxiv - Cell Biology 2023Quote: ... This was followed by overnight incubation at 4°C with 1:200 anti-LAMP-1 (D401sm #15665, Life Technologies). The next day ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated with rocking for 1 h at 4 °C with 1 mL HisPur Ni-NTA resin (ThermoFisher Scientific) pre-equilibrated in SEC buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... 2011)) at 1:400 concentration at 4°C and secondary antibodies Alexa Fluor 647 (1:500; Thermo Fisher Scientific) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... carrying the mutation R273C was first amplified by PCR using the primers hp53-1 (5’-CACCATGGAGGAGCCGCAGTCAGATCC-3’) and hp53-8 (5’-GGATCCTCAGTCTGAGTCAGGCCCTTCTGTCTTG-3’) and cloned into the pENTR/D-TOPO vector (ThermoFisher) generating the entry vector pENTR p53(R273C ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of 100 μL was subsequently derivatized using a final concentration of 10 mM aniline and 5 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (ThermoFisher) for 2 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... 25 μl/well of 60 mM water solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Thermo Fisher Scientific) were added ...
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bioRxiv - Immunology 2022Quote: ... with siRNAs against SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA (Ambion). Three days after transfection with either siRNA or shRNA plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... The carboxylic groups on the carbon fiber surface were electro-activated by incubation in 0.4 M 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC; Life Technologies) and 0.1 M and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Neuroscience 2022Quote: ... the sgRNA sequence targeting exon 1 of Faah (5’-CTGCAGGCTAGGCAAACC-3’) and a control sgRNA sequence (5’-CTGCAGGCTAGGCAAACCTTT-3’ were synthesized (Invitrogen) and cloned into the shuttle plasmid for adeno-associated viral (pAAV-FLEX-SaCas9-U6-sgRNA;Addgene #124844 ...
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bioRxiv - Microbiology 2023Quote: ... or alkaline phosphatase was added at a 1:2,000 dilutions for 1 h at 37ºC followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Scientific) or p-nitrophenyl phosphate (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... Monoclonal antibodies were diluted to 30 μg/mL and then serially diluted 1:3 to a final concentration of 1.52x10-3 μg/mL in PBST supplemented with 1% non-fat milk (Life Technologies). Following blocking ...
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bioRxiv - Bioengineering 2020Quote: ... methyl ester (TMRM) (0.1µM in hepatocyte medium, Thermo Fisher) to visualize active mitochondria ...
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bioRxiv - Neuroscience 2022Quote: ... containing 80μL N-methyl-trimethylsilyl-trifluoroacetamide (MSTFA; ThermoFisher #TS48915) and gently vortexed followed by 30 min dry heat incubation at 37°C ...