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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for 2 Chloro 3 6 chlorohexanoyl pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Reverse 5′-CGA AGG TGT GAC TTC CAT G-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Cell Biology 2022Quote: HCT116 cells were cultured in a 6-well plate at 300,000 cells per well in 3 ml McCoy’s 5A medium (Gibco) supplemented with 10% heat inactivated FBS and with 90 UI/mL Penicillin ...
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bioRxiv - Neuroscience 2020Quote: Neocortex from C57Bl/6 pups (postnatal day 1-3) was dissected in Hibernate-A medium (#A1247501, Gibco, USA), digested with papain (#LS003124 ...
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bioRxiv - Cell Biology 2022Quote: ... For the bulk endocytosis experiments using the 4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]-pyridiniumbromide dye (FM 4-64, Invitrogen), the cells were prepared as previously described [41] ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 30 ovaries from 3-6 day-old flies using TRIzol (Thermo Fisher Scientific) in three replicates ...
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bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: After a wash in Carnoy’s solution (6 ethanol: 3 chloroform: 1 acetic acid; all from Thermo Fisher, USA), used to removed most of the lipids from the section [45] ...
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bioRxiv - Neuroscience 2024Quote: We derivatized the dried polar and protein samples using methoxyamine HCl (20 mg/mL) in pyridine and N-methyl-trimethylsilyl-trifluoroacetamide (MSTFA; Thermo Fisher Scientific). For the polar fraction ...
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bioRxiv - Neuroscience 2022Quote: ... 2.8×106 neural progenitor cells were grown on glass coverslips in 6-well plate and loaded with 2 µM Fura-2/AM and Pluronic F127 in OptiMEM (Thermo Fisher Scientific) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryoEMbioRxiv - Immunology 2024Quote: ... Cells were resuspended in phosphate buffered saline(PBS) + 2% fetal bovine serum(FBS) 200 ng/mL 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific). Cell sorting was performed on a BD FACSAria Fusion (BD Biosciences).
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bioRxiv - Bioengineering 2024Quote: ... for 2 h and a 1 µg mL−1 solution of 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific 62248) for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then washed in PBST-2 (PBS containing 0.1% Triton X-100) for 3 × 2 minutes and blocked with SeaBlock blocking buffer (ThermoFisher) for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were first loaded for 3 min onto the Acclaim PepMap 100 C18 trap column (75 µm x 2 cm, 3 µm material, Thermo Scientific) with 5 µL min-1 of 3.2% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... with single-cell passaging every 2-3 days using accutase (ThermoFisher Scientific A1110501) at split ratios between 1:5 and 1:12.
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bioRxiv - Plant Biology 2020Quote: ... from 2-3 weeks old seedlings and 500ng was treated with DNaseI (Ambion) and used for reverse transcription ...
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bioRxiv - Neuroscience 2021Quote: ... TRPA1−/− male mice were loaded with Fura-2 (3 μg/mL, Life Technologies) and pluronic acid (1:1 ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 2 mM caspase-3/7 detection reagent (Fisher Scientific) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... were all purchased from Sigma and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium was purchased from Thermofisher.
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bioRxiv - Biochemistry 2020Quote: ... and cells were loaded with 3 μM Fura-2 AM dye (Molecular Probes) in sterile-filtered HEPES-Tyrode Buffer (HTB ...
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bioRxiv - Cell Biology 2021Quote: ... Primary cells were loaded with Fura-2 AM (3 μM; Invitrogen/ThermoFisher Scientific) for 45 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Primary cells were loaded with Fura-2 AM (3 μM; Invitrogen/ThermoFisher Scientific) for 45 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... p97 siRNAs (2-HSS111263 and 3-HSS111264) were from Invitrogen (Thermo Fisher Scientific). p97 rescue constructs were previously published 53 and were resistant to siRNA # 2 ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 and Caco-2 cells were cultured in minimum essential medium (GIBCO) while A549-ACE2 cells were maintained in DMEM/F-12 medium (GIBCO) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP) (Thermo Fisher Scientific, USA) in PBS (Thermo Fisher Scientific ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) (M6494, Thermo Fisher Scientific) was added to each well to a final concentration of 0.67 mg/ml and incubated at 37oC ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP, Thermo Fisher Scientific, USA) in PBS (Thermo Fisher Scientific ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Cells were passaged every 2-3 days using 0.05% Trypsin-EDTA (Gibco, 25300062). These cells were cultured in Knockout™ D-MEM (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Capan-2 and BxPC-3 cell lines were cultured in RPMI medium (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Genomics 2024Quote: ... Cells were passaged every 2-3 days using 0.25% trypsin-EDTA (#25200056, Gibco) to keep them below 80% confluency ...
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bioRxiv - Immunology 2023Quote: ... and the pellet resuspended in 2-3 mL of TrypLE Express (Gibco # 12605010) for 15-20 min at 37°C to dissociate the organoids into single cells ...
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bioRxiv - Immunology 2023Quote: ... and the pellet resuspended in 2-3 mL of TrypLE Express (Gibco # 12605010) for 12 min at 37°C to keep the LPC spheroids as aggregates ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher Scientific). Images were captured automatically every two hours for 48 hours using the IncuCyte™ S3 Live-Cell Analysis Instrument (Essen BioScience) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following 3 days of selection with 2 µg/ml puromycin (Gibco, A11138-03), reference samples were collected (t=0 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 2-3 hours at room temperature and incubated with Hoechst 33342 (Invitrogen) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... and treated with 2 µM Caspase-3/7 Green detection reagent (C10423, Invitrogen) along with the specified compounds ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged every 2-3 days using TryplLE™ express enzyme (GIBCO) and trypsin inhibitor (GIBCO).
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bioRxiv - Genetics 2023Quote: ... each coverslip was incubated in 3 μl fura-2 acetoxymethyl (AM) ester (Invitrogen) in 1X HBSS containing 5 mg/ml BSA (Sigma- Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... To this mixture we added glycerol 2-phosphate (3 M, Thermo Fisher Scientific) and (GT)15-SWCNTs (3.75 mg/L ...
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bioRxiv - Plant Biology 2024Quote: ... 2-3 µg of total RNA were treated with DNase I (Thermo fisher), and half was reverse transcribed using the SuperScript™ IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Cells were loaded with 2 µg/ml Fluo-3 AM (Thermo Fisher Scientific) and 5 µg/ml Fura Red AM (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The final products were visualized via gel electrophoresis using 2-3% Agarose (Invitrogen) gels ...
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bioRxiv - Microbiology 2024Quote: ... which was then transferred to 2-3 mL TRIzol Reagent (Invitrogen, Waltham, MA) to form a slurry and finally thawed ...
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bioRxiv - Physiology 2020Quote: ... interleukin 6 (IL-6; Invitrogen, Carlsbad, CA), plasminogen activator inhibitor 1 (PAI-1 ...
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bioRxiv - Biochemistry 2024Quote: ... dsRNA complex (2:1 molar ratio) were cross-linked with 30 μg 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific, EDC: protein = 3:1 w/w) in the presence of 66 μg N-hydroxysulphosuccinimide (NHS ...