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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for 2' Methyl 3 phenylpropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 μg DNA) and β-arrestin-2-mCherry (1 μg DNA) using 3 μL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown in cell culture dishes (Falcon) and passaged every 2-3 days via trypsinization with 0.0S% Trypsin-EDTA (2S300-0S4, Thermo Fisher Scientific) at 80-90% confluency ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of peptide mixtures (1µg) were loaded onto an Acclaim PepMap precolumn (75 µm x 2 cm, 3 µm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap RSLC C18 Easy-Spray column (75 µm x 50 cm ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 g of flash-frozen human prefrontal cortical post-mortem tissue was sliced with a razor blade into 2–3 mm3 sections in Hibernate-E media (Thermo Fisher) on ice ...
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bioRxiv - Immunology 2024Quote: ... the reaction mixture was dialyzed against deionized water at 4 °C for 2 days using Slide-A-LyzerTM Dialysis Cassette (10K MWCO, 3 mL, Thermo Scientific) and stored at −70 °C.
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bioRxiv - Biophysics 2024Quote: ... The grids were blotted for 3 seconds at 100% humidity with force “2” and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 9496 Movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Biochemistry 2024Quote: ... which was equipped with a 75 μm x 2 cm trap column packed with C18 3 μm bulk resins (Acclaim PepMap 100, Thermo Scientific) and a 75 μm x 15 cm analytical column with C18 2 μm resins (Acclaim PepMap RSLC ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM ATP and 1% fraction V BSA) and loaded with 3 μM Mag-Fura-2 AM (Thermo Fisher Scientific, #M1292) at room temperature for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... onto a nanotrap column (75 μm i.d. x 2 cm precolumn, packed with Acclaim PepMap100 C18, 3 μm, 100 Å; Thermo Fisher) using an EASY-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.05 M TBS and then incubated in the dark for 2 h at room temperature with secondary antibodies: donkey anti-mouse Alexa Fluor 488 (3 µl/ml; Life Technologies) and donkey anti-rabbit Alexa Fluor 594 (5 µl/ml ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were aliquoted in 10 mL (OD600 ∼ 0.2) and re-grown for 2-3 h to extract plasmids using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) for sequencing and for subcloning into the expression strain ...
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bioRxiv - Microbiology 2024Quote: ... the samples were stained with 80 μL CellEvent Caspase 3/7 Green detection Reagent 2 µM in PBS with 5% FBS (Invitrogen, C10423) and then incubated for 30 minutes at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peptides were first loaded on the trap column (Acclaim PepMap 100 C18, 75 µm ID x 2 cm, 3 µm particle size, Thermo Scientific), washed with 0.1 % formic acid in water for 7 min at 5 µL/min and subsequently transferred to the separation column (IonOpticks Aurora C18 column ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were routinely passaged every 2-3 days by washing twice with PBS lacking divalent cations and treating with StemPro Accutase (ThermoFisher A1110501).
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bioRxiv - Neuroscience 2024Quote: ... DAPI was allowed to dry for approximately 3 minutes before immediately coverslipping with 1-2 drops of ProLong Gold (Invitrogen, P36930). Slides were protected from any surrounding light ...
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bioRxiv - Microbiology 2024Quote: ... Plates were placed in sealed boxes to prevent from drying of the perimetric wells and incubated without shaking at 37°C for 6 days before addition of a mix solution of 20% tween 80 plus MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (Stock 5 mg/mL, Acros Organics, Ref. 15224654) or the Bactiter-Glo Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed once with PBS and 3 mL of DMEM (2% FCS) containing 1% low melting point agarose (ThermoFisher Scientific) was added to each well ...
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bioRxiv - Biophysics 2024Quote: ... The grids were blotted for 3 seconds at 100% humidity with force “2” and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 7376 movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... The grids were blotted for 3 seconds at 100% humidity with force “2” and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 41335 movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Systems Biology 2024Quote: ... and incubated in total of 150 µL SH2 binding buffer containing 20 µM biotinylated SH2 domain on a rotator and 4 °C for 2-3 hours in low protein binding microcentrifuge tubes (1.5 mL, Thermo Scientific™). The functionalized magnetic beads are then washed twice in 1 mL of SH2 binding buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... with an in-line Acclaim PepMap 100 C18 trap column (75 μm × 2 cm, 3 μm, 100 Å, Thermo Fisher Scientific) heated to 45 °C ...
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bioRxiv - Cell Biology 2024Quote: ... were plated onto 6-well culture plates at 2×105 cells/well (concentration determined using the Countess 3 automated cell counter; Invitrogen: AMQAX2000) for downstream experiments (n=5 biological replicates/treatment) ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were acidified to pH 2-3 using trifluroacetic acid (TFA) prior to LC-MS analysis on a Dionex U3000 UHPLC system (ThermoFisher Scientific) equipped with a column chromatography setup coupled to a Q Exactive Plus Orbitrap MS (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: Samples were washed 3 times with DPBS before being incubated with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, 10125092) at 1:500 in the blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown to a confluency of 70-90% and passaged every 2-3 days via trypsinization with 0.05% Trypsin-EDTA (25300-054, Thermo Fisher Scientific). Cell lines were regularly checked for Mycoplasma contamination and confirmed negative ...
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bioRxiv - Genomics 2022Quote: ... Bisulfite-PCR was conducted using primer sets (Integrated DNA Technologies; Table 1) designed against specific genomic regions with Methyl Primer Express v1.0 software (Thermofisher Scientific). PCR amplicons were cleaned with AmpureXP beads (#A63882 ...
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bioRxiv - Biochemistry 2020Quote: ... N,N-dimethyl formamide (DMF) and water and HPLC grade methyl tert-butyl ether (MTBE) were obtained from Thermo Fisher used throughout ...
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bioRxiv - Systems Biology 2021Quote: ... A total of 90 μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (Thermo Scientific, Lafayette, CO, U.S.A.) was then added to the samples and shaken at 1400 rpm for 30 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dissolved in 300 μl of 6:1 (v/v) of methyl sulphoxide D6 (99.9% atom D + 1% tetramethylsilane, ACROS Organics):D2O (100% atom D ...
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bioRxiv - Biochemistry 2021Quote: The KDELR/Legobody complex at 0.8mg/ml was PEGylated by incubation with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher) at a 1:40 molar ratio for 2 hrs on ice to reduce preferred particle orientation on the grids ...
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bioRxiv - Microbiology 2023Quote: ... aeruginosa growth support by pyochelin and pyochelin methyl ester were performed in M9 minimal medium supplemented with 500 µM 2,2’-Bipyridine (Fisher Scientific). M9 minimal medium was prepared as follows ...
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bioRxiv - Biochemistry 2022Quote: Changes in the mitochondrial membrane potential were determined as the changes in tetramethylrhodamine methyl ester (TMRM, T668, Thermo Fisher Scientific) fluorescence according to the manufacturer’s instructions with few modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured for 24 hours in culture medium supplemented with 0.5% (w/v) methyl cellulose and B-27 (1/50, Life technologies).
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
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bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
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bioRxiv - Immunology 2022Quote: ... 1.9g of ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) (ThermoFisher #22980) and 1.2g of N-hydroxysuccinimide (NHS ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Cell Biology 2024Quote: ... human custom-made 3’UTR HEC1 siRNA (oligo #3, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Scientific, 22980) were added to the alginate solution in a molar ratio of 1 alginate:30 NHS:25 EDC ...
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bioRxiv - Biophysics 2024Quote: ... and 3-pyridinemethanol (RI = 1.545, 3-PM, A10381, Thermo Fisher Scientific) were used to mix with the water-based SMLM buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and TagRFP-T-VAPB(1-218)-eMagB or TagRFP-T-eMagB-PHOSBP (prey) and iRFP-P4C at a 3:2:1 ratio in OptiMEM-I (Thermo Fisher Scientific) (1:4 DNA ...
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bioRxiv - Cell Biology 2020Quote: Total cellular mRNA was isolated from cells grown in 2-D and 3-D culture conditions using the GeneJET RNA purification kit (Thermo Fisher Scientific). For analysis of germ layer markers ...
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bioRxiv - Cell Biology 2020Quote: An aliquot of each sample equivalent to 3 μg was loaded onto a trap column (Acclaim PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (Acclaim PepMap RSLC column C18 2 μm ...
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bioRxiv - Biochemistry 2022Quote: ... The trap column used for the nano HPLC was a 2 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Thermo Fisher Scientific) and the separation column was a 12.5 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Nikkyo Technos Co. ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3.5 minutes at 5 μL/min with 2% Acetonitrile MS grade (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... were concentrated and desalted on a trapping pre-column (Acclaim PepMap C18, 75 µm × 2 cm nanoViper, 3 µm, 100 Å, Thermo Scientific), using 0.2% formic acid at a flow rate of 5 µl/min ...
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bioRxiv - Neuroscience 2020Quote: ... and then 12 µg from each was injected into a 75 μm × 2 cm nanoViper C18 Acclaim PepMap100 precolumn (3 μm particle size, 100 Å pore size; P/N 164705; Thermo Scientific). Peptides were separated at a flow rate of 250 nl/min and eluted from the column over a 5 hours gradient starting with 0.1% FA in ACN (5-30% ACN from 0 to 280min followed by 10min ramping up to 80% ACN ...