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Citations for Thermo Fisher :
7401 - 7450 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... LX-2 cells were cultured in DMEM (Gibco 11965092) supplemented with 1% penicillin/streptomycin (Gibco 15070063) ...
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bioRxiv - Cell Biology 2024Quote: ... containing 2% B27 without vitamin A (Thermo Fisher Scientific), 200 µM ascorbic acid (MilliporeSigma ...
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bioRxiv - Biophysics 2024Quote: ... 2 mM GlutaMAX Supplement (Thermo Fisher Scientific 35050-038).
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bioRxiv - Developmental Biology 2024Quote: ... Dissected frontonasal pieces were incubated in 2% trypsin (Gibco) at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... into the medial septal area (MS) of C57Bl/6NTac mice (n=6). For the colocalization experiments (Fig. 1) we injected yellow-green FluoSpheres (Thermo Fisher Scientific) into the MS of two C57Bl/6NTac mice or AAVrg-syn-FLEX-jGCaMP8m-WPRE (Addgene ...
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bioRxiv - Genetics 2020Quote: ... Zygotes were then cultured in the chemically defined hamster embryo culture medium-9 (HECM-9) containing 10% fetal bovine serum (FBS, GIBCO) at 37 °C in 5% CaO2 to allow embryo development ...
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bioRxiv - Cell Biology 2022Quote: ... Myc or FLAG-tagged procaspase-9 plasmid was prepared by cloning the procaspase-9 gene into a pcDNA-based vector (Invitrogen). Procaspase-9 with three mutations (Asp315Ala ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was discarded and the pellet was resuspended in 5 mL liquid KNOP supplemented with 2% (w/v) sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418 ...
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bioRxiv - Cell Biology 2022Quote: ... and washed for 3 min on a C18 reverse-phase precolumn (3 μm particle size, 100 Å pore size, 75 μm inner diameter, 2 cm in length, from Thermo Fisher Scientific, Waltham, MA, USA). Peptides were separated on an Aurora C18 reverse-phase resin (1.6 μm particle size ...
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bioRxiv - Cell Biology 2022Quote: Transient genetic silencing of HOIP and FLOT1/2 was performed by reverse transfection of HT-29 cells with the following siRNAs: human siHOIP (RNF31) (Silencer® Select siRNA #1 s30108, #2 s30109, #3 s30110, 40 nM) (Thermo Fisher Scientific, Waltham, MA, USA), human FLOT1 (ON-TARGETplus Human FLOT1 siRNA SMART POOL ...
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bioRxiv - Cancer Biology 2024Quote: ... an additional 150 µL of a 1:1 mixture of NK-Xpander complete medium:OncoPro complete medium containing 500 U/mL rhIL-2 and 10 µM Invitrogen™ CellEvent™ Caspase-3/7 Red Detection Reagent (Thermo Fisher Scientific, cat# C10430) was added to each well ...
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bioRxiv - Synthetic Biology 2021Quote: ... Calcofluor white was purchased from Sigma-Aldrich and SYTO 9 was purchased from Thermo Fisher Scientific.
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bioRxiv - Biophysics 2022Quote: ... supplemented with 9% Fetal Bovine Serum (FBS, Gibco) and a penicillin/streptomycin mixture (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... using 9 uL of Lipofectamine RNAiMAX (Life Technologies). After 40 hours post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... SYTO-9 (ThermoFisher, #S34854; final concentration: 2.2 μM) was used ...
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bioRxiv - Developmental Biology 2022Quote: ... for mature miR-9 or SuperScript III (Invitrogen) with random hexamers for pri-miRNAs ...
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bioRxiv - Microbiology 2019Quote: ... syto-9 and propidium iodide (Life technologies(tm)) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The diluent was HMI-9 media (Invitrogen, UK). Blood stream form T ...
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bioRxiv - Cell Biology 2021Quote: ... a 7-9% agarose (Invitrogen, cat no. 16500500) pad made from the imaging media was gently placed over the top of the worms ...
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bioRxiv - Cancer Biology 2021Quote: ... UD cells were cultured in LHC-9 (Gibco) supplemented with 1% Pen/Strep ...
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bioRxiv - Cell Biology 2019Quote: ... caspase-9 (10 µM ZLEHD-R110; Molecular Probes), or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence ...
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bioRxiv - Bioengineering 2022Quote: SYTO® 9 (Thermo-Fisher Scientific, Waltham, MA) was used to fluorescently stain nucleic acid of the human oral microbiome pooled samples to investigate viability at 24 h in the anatomical gingival tissue model in comparison to the planar construct ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... supplemented with 9 % heat inactivated FBS (Gibco 10500064) at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... the epithelium was stained with Syto 9 (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... combined with 9 µl lipofectamine 2000 (Invitrogen 11668019). Transfection mixtures were incubated at room temperature for 15 mins ...
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bioRxiv - Biophysics 2023Quote: ... 0.8 µL crosslinker BS(PEG)9 (Thermo scientific) and 10 μM protein were incubated for 1 hour at 4℃ and filtered by Superose 6 in buffer (20 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... before washing in PBST and addition of substrate (OPD, O-phenylenediamine dihydrochloride, Thermo Scientific). The reaction was stopped using 10% H2SO4 and read at A492nm.The formation of FH-thrombin complex in normal human plasma was measured by adding 1μM PPACK-thrombin to 50μl EDTA-plasma containing endogenous FH and quantified using a FH-thrombin standard curve.
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bioRxiv - Cancer Biology 2020Quote: ... cells were cultured in medium containing 1 µg/ml Puromycin Dihydrochloride (Thermo Fisher Scientific) 48 hours after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Dihydrochloride (DAPI) and measured using Attune Nxt acoustic focusing cytometer (Life Technologies, Carlsbad, USA).
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bioRxiv - Microbiology 2022Quote: ... and 100 μl of o-phenylenediamine dihydrochloride (OPD) substrate (Thermo Scientific, 34006 and 34062) was added to each well ...
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bioRxiv - Neuroscience 2022Quote: ... to induce TetO gene expression and Puromycin Dihydrochloride (1 ng/ml, Thermo Fisher Scientific) to select only the genetically engineered cells.
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bioRxiv - Developmental Biology 2020Quote: Islet cell clusters were incubated (25 min at 37 °C) in RPMI supplemented with 2 μM Fura-2 acetoxymethyl ester (Molecular Probes, Eugene, OR). Fluorescence imaging was performed using a Nikon Eclipse TE2000-U microscope equipped with an epifluorescence illuminator (SUTTER ...
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bioRxiv - Pathology 2020Quote: ... or Abberior STAR635P (Abberior, 2-0142-007-2, dilution 1:50) and donkey anti-rabbit conjugated to Alexa-555 (Thermo Fisher, A-31572) or Cy3 (Jackson Immuno Research ...
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bioRxiv - Microbiology 2021Quote: ... cells in each well were infected with 2000 TCID50 SARS-CoV-2 pseudovirus in 100 µL of culture medium containing 2% FBS (Gibco, Cat. No. 10099141C). Culture medium was refreshed every 12 hours ...
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bioRxiv - Immunology 2022Quote: ... and transferred using electrophoresis to nitrocellulose membranes by using the iBlot 2 Gel Transfer Device (Thermo Scientific, iBlot 2 NC Regular Stacks, IB23001, Invitrogen, Walham, MA). Membranes were blocked for 1 h at RT in 5% (w/v ...
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bioRxiv - Microbiology 2022Quote: Primary human CD4+ T cells were incubated with 20U/ml interleukin-2 (IL-2) alone or in combination with α-CD3/CD28 antibody-coated beads (100 μL/30 x 106 cells) (Gibco/Thermo Fisher) for 72 hours prior to infection with 100ng of p24 equivalent of pseudotyped pMorpheus-V5 ...
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bioRxiv - Immunology 2021Quote: ... 200μL of platelet suspension (2×106 platelets in HBSSCa-Mg-) from healthy donors was labelled with calcein-AM (2 μg/mL final concentration, ThermoFisher Scientific Cat # C3100MP) for 15 mins and then R406 ...
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bioRxiv - Neuroscience 2022Quote: ... with 16 μM BSA in the presence of CaCl2 (1.3mM) and fura-2-acetoxymethyl ester (fura 2-AM, 5 μM: Molecular Probes, Eugene, OR, USA), and they were incubated at 37 °C for 25 min ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Immunology 2020Quote: ... Purified monoclonal antibody stock at a concentration of approximately 1mg/mL was 1:2 serially diluted in infection media (DMEM with 2% FBS and 1X penicillin-streptomycin (Thermo Fisher catalog 15140122) starting at 1:20 and ending at a dilution of 1:20,480 ...
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bioRxiv - Microbiology 2022Quote: ... and the residual bone marrow cells were plated in tissue-culture-treated microplates at a density of 2 × 105 cells per well in RPMI 1640 containing 2 mM glutamine (Thermo Fisher Scientific, 21875034), 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: To assess endothelium barrier function we perfused microvessels with 2 μM Alexa Fluor647-conjugated 2 MDa dextran (Thermo Fisher Scientific, cat. no. D22914) in EGM-2 medium 48 h following inoculation with Bb or vehicle (BSK-II medium ...
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bioRxiv - Neuroscience 2022Quote: ... both proximal and distal chambers were washed with HibE imaging medium (Hibernate E [BrainBits] supplemented with 2% B-27, 2 mM GlutaMAX, 100 U/ml penicillin and 100 µg/ml streptomycin [Thermo Fisher Scientific/Gibco; 15140–122]) twice while maintaining fluidic isolation ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were loaded with 5 μM acetoxymethyl-ester of Fura-2 (Fura-2/AM) and 80 μg /ml pluronic F127 (Molecular Probes, Eugene, Oregon) for at least 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies (AlexaFluor546 anti-mouse, ThermoFisher, cat: A-11030, at 2 µg/mL; AlexaFluor647 anti-rabbit, ThermoFisher, cat: A32733, at 2 µg/mL) were incubated 2h/shaking at RT (BSA at 2% ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with 400 ng/well 2-E plasmids or 2-E mutations using Lipofectamine 3000 Transfection Reagent (L3000015, Thermo Fisher, MA, USA). The control group was Vero E6 cells transfected with 400 ng/well pcDNA3.1 vector plasmids alone ...
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bioRxiv - Immunology 2024Quote: ... and SARS-CoV-2 S protein were immobilized at 2 μg/ml on 96-well polystyrene microtiter plates Nunc MaxiSorp™ (Thermo Fisher Scientific). Following blocking with 1 × synthetic block (Thermo Fisher Scientific ...