Labshake search
Citations for Thermo Fisher :
7351 - 7400 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... 1:500) diluted in 1% BSA in PBS with 160U/mL RNaseOUT (Invitrogen) overnight at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... REP medium consisted of a 1:1 mix of RPMI (Gibco, Cat. 7001612) and X-Vivo (Lonza ...
-
bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500; 2 mg/mL Alexa Fluor 568, Invitrogen, 1:1000) diluted in PBS for 1.5 h at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... and hTERT RPE-1 (CRL-4000; ATCC) were cultured in Corning DMEM/Ham’s F-12 1:1 mixture (Fisher Scientific, Waltham, MA) with 10% FBS (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... Dead cells were excluded by labeling using PI (1 mg/ml, 1:500) or near IR-fixable dye (1:1000, Invitrogen, cat. L34976). For the wash step ...
-
bioRxiv - Biophysics 2022Quote: ... The cell culture medium (RPMI 1640) and complements (10% FBS, 1% Hepes 1M, 1% Glutamax, 1% Pen/Strep) were obtained from Gibco (Life technologies). Cells were monthly tested for the presence of mycoplasma.
-
bioRxiv - Systems Biology 2022Quote: ... Cells were immunostained according to standard protocols [21] using a primary rabbit anti-human antibody directed against zona occludens-1 (ZO-1; 1:100, Thermo-Fisher Scientific, UK) and a AF488-conjugated secondary goat anti-rabbit antibody (1:500 ...
-
bioRxiv - Bioengineering 2022Quote: ... Prepared drug stocks were then diluted 1:100 in cell maintenance media (Gibco DMEM supplemented with 10% heat-inactivated FBS, 1% Gibco NEAA, 1% Gibco Pen-Strep) to achieve a 2x concentration ...
-
bioRxiv - Developmental Biology 2020Quote: ... Pros (mouse 1:100, DSHB MR1A), Hairless (guinea pig 1:500, Annett and Dieter) and V5 (mouse 1:500, Invitrogen R960-25).
-
bioRxiv - Biochemistry 2020Quote: ... wells were appropriately treated with anti-rabbit 594 antibody (1:1200, MilliporeSigma) for 1 hour at room temperature and then DAPI (1:1000, Thermo Fisher Scientific) for 15 minutes at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... and HA-mUNC79 plasmids or their various mutants (in a modified BacMam expression vector 27) at a ratio of 2:1:1:1 using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 24 hours before recording ...
-
bioRxiv - Cell Biology 2022Quote: ... Each cell lysate was mixed with Cellular ATP Kit HTS (155-050, BioThema) in the ratio of 1:1 and luminescence was analyzed using Microlite™ 1+ strips (Thermo Scientific) and Chameleon V (Hindex).
-
bioRxiv - Molecular Biology 2022Quote: ... pLV-mCherry at ratio 1:1:1 (i.e. 5:5:5 μg) using Lipofectamin™ 3000 transfection reagent (Thermo-Fisher Scientific, #L3000015). Pseudoviruses harvested from the supernatant at 48 h 72h post-transfection were filtered (0.44 μm ...
-
bioRxiv - Biophysics 2022Quote: ... and 1% penicillin/streptomycin (final concentration of 100 IU mL−1 penicillin and 100 μg mL−1 streptomycin, Gibco, Thermo Fisher, USA). Dry PIC polymers were sterilized by UV for 10 min and then dissolved in the medium for 24 h at 4 °C ...
-
bioRxiv - Biophysics 2022Quote: ... and 1% penicillin/streptomycin (final concentration of 100 IU mL−1 penicillin and 100 μg mL−1 streptomycin, Gibco, Thermo Fisher, USA). Dry PIC polymers were sterilized by UV for 10 min and then dissolved in the medium for 24 h at 4 °C ...
-
bioRxiv - Genetics 2021Quote: ... and stained with rabbit polyclonal antibodies against collagen XII (KR144;1:200), collagen VI (1:1000, gift from Raimund Wagener, Cologne) and Alexa 488 conjugated wheat germ agglutinin (WGA) (Invitrogen, 1:500), followed by corresponding secondary antibodies coupled to Cy3 (1:800 ...
-
bioRxiv - Immunology 2020Quote: ... containing 1 µl 10x DNase I reaction buffer and 1 µl (1 U/µl) amplification grade DNase I (both from Invitrogen, Carlsbad, USA). The resulting mix was incubated for 15 min at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... fluence 0.6 μmol m−2 s−1) XccBphP wild-type samples at 1 mg mL−1 (14 μM) were incubated with bissulfosuccinimidyl suberate (BS3) (Thermo Fisher Scientific) in 250 ...
-
bioRxiv - Cell Biology 2020Quote: ... cut into 1 × 1 cm pieces and incubated overnight in digestion medium containing 1 mg/ml collagenase type I (Gibco, Massachusetts, USA) and 100 μl/ml antibiotic/antimycotic solution in Modified Hank’s Balanced Salt Solution (HBSS ...
-
bioRxiv - Immunology 2019Quote: ... in a 1:1 beat to T-cell ratio and cultured in 2 ml (24WP) or 1 ml (48WP) RPMI 1640 (Thermo Fisher, 21875) supplemented with 10% Fetal Bovine Serum ...
-
bioRxiv - Cell Biology 2021Quote: ... Primary antibodies Anti-FAS3 (Developmental Biology Hybridoma Bank (DSHB); 1:10) and anti-EcR (DSHB; 1:10) were used in conjunction with fluorescent Alexa-555- (ThermoFisher; 1:400) and Cy3- (Jackson Laboratories ...
-
bioRxiv - Systems Biology 2020Quote: RNA-Seq libraries were prepared from 65 ng of total RNA and 1 μL of 1:5000 (v/v) dilution of ERCC ExFold RNA Spike-In Mix 1 (Thermo Fisher Scientific) with TruSeq® Stranded mRNA Library Prep for NeoPrep™ instrument as per the NeoPrep Library Prep System Guide on the instrument (Illumina).
-
bioRxiv - Synthetic Biology 2020Quote: ... were grown on 0.1% gelatin-coated 35mm cell culture dish in EGM2 media (20% Fetal Bovine Serum, 1% penicillin-streptomycin, 1% Glutamax (Gibco, catalog #35050061), 1% ECGS (endothelial cell growth factors) ...
-
bioRxiv - Cell Biology 2019Quote: ... For the negative control primary antibodies mouse anti-plasmepsin V (From D. Goldberg, 1:1) and rabbit anti-GFP A-6455 (Invitrogen, 1:200) were used.
-
bioRxiv - Bioengineering 2021Quote: ... The primate ovaries were dissected into approximately 60 fragments measuring 1×1×1mm (1 mm3) and transferred into maintenance media (α- MEM, P/N 32561, Gibco, USA) prior to encapsulation (Figure 4) ...
-
bioRxiv - Biochemistry 2021Quote: The three plasmids encoding the CST subunits were transfected into HEK293T cells at 1:1:1 molar ratio using lipofectamine 2000 (11668019, Thermo Fisher Scientific). The cells were further expanded (typically three-fold ...
-
bioRxiv - Cell Biology 2020Quote: ... Droplets were solidified at 37°C and were grown four days without agitation in neurosphere medium containing a 1:1 mixture of DMEM/F12 and Neurobasal supplemented with N2 (1:200) (Thermo Fisher Scientific), B27 (1:100 ...
-
bioRxiv - Immunology 2020Quote: ... T cell activation was performed using plate-bound α-CD3 (clone 145-2C11, 1 μg/ml) and α-CD28 (clone PV-1, 1 μg/ml) monoclonal antibodies in RPMI 1640 medium (Life Technologies) supplemented with 10% FCS ...
-
bioRxiv - Molecular Biology 2020Quote: ... Rabbit antibody to pSMAD 1/5/8 (1:100; CST 9516) and mouse antibody to beta-amyloid (1:100; Invitrogen 13-200) were diluted in the same 3% blocking buffer and incubated overnight at 4°C ...
-
bioRxiv - Bioengineering 2022Quote: ... secondary antibodies (osteopontin: Alexa 546, 1:200, A21123, Invitrogen Molecular Probes; collagen type 1: Alexa 647, 1:200, A21246, Invitrogen Molecular Probes) were incubated with 0.1 μg/ml DAPI for 1 h at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: ... secondary antibodies (osteopontin: Alexa 546, 1:200, A21123, Invitrogen Molecular Probes; collagen type 1: Alexa 647, 1:200, A21246, Invitrogen Molecular Probes) were incubated with 0.1 μg/ml DAPI for 1 h at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: ... and subsequently incubated in primary antibody solution (1:200 CD31 Dako IS610 + 1:200 CD10 Invitrogen PA5-85875 or 1:200 anti-laminin Abcam ab11575 or 5 μg/mL ZO-1 Invitrogen #61-7300) overnight at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... incubated overnight with primary antibodies (1:5000 α-TNY1, 1:10,000 Roche Rat-anti-HA, high affinity 3F10, or 1:50,000 Invitrogen α-Histone H3) in 5% non-fat dry milk ...
-
bioRxiv - Immunology 2022Quote: ... mBECs treated with IFNγ were fixed with 4% paraformaldehyde in PBS for 15 minutes and stained with antibodies against Zona occludens-1 (ZO-1, 1:250 Invitrogen 33-9100) and CXCL10 (1:500 Abcam ab9938) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell lines were injected sub-cutaneously into the flank of adult mice at 1-5×106 cells per injection in a 1:1 mixture of serum-free Opti-MEM (Gibco, 31985-070) and Matrigel (Corning ...
-
bioRxiv - Cancer Biology 2022Quote: ... complimentary to Ov-grn-1 exon 1 termed pCas-Ov-grn-1 was constructed using the GeneArt CRISPR Nuclease Vector kit (Thermo Fisher Scientific), as described ...
-
bioRxiv - Cell Biology 2022Quote: HUVECs expressing PIK3CA p.H1047R or PIK3CA wt were transfected with a Silencer Select siRNA targeting to TGFA (ID: s14053) or negative control siRNAs (#1 and #2 mixed in ratio 1:1; all siRNA oligonucleotides from Thermo Fisher Scientific) as previously described (58) ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were blocked with 1% BSA/5% Goat Serum solution for 1 hour at room temperature and then stained with anti-ZO-1 antibody (1:200; Invitrogen; Carlsbad, CA) for pneumocytes and anti-VE-cadherin (1:100 ...
-
bioRxiv - Cell Biology 2024Quote: ... The plate was blocked with 1% BSA in PBS for 1 h and then incubated with anti-MTCO2 (1:200 dilution, clone 12C4F12, Thermo Fisher Scientific) or anti-Cox6 (1:200 dilution ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in rabbit polyclonal anti-Blbp (Suppl. Table 1) overnight followed by 1 hr in donkey anti-rabbit 555 (1:800; Life Technologies, #A31572) and coverslipped with Fluorogel ...
-
bioRxiv - Developmental Biology 2024Quote: Ovaries from 5-7 females were dissected in 1% PBS-Tween and fixed in a 1:1 mixture of 4% formaldehyde (Thermo Scientific, 28908) and heptane (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... rabbit anti-akt (9272, CellSignaling, 1:1000), rabbit anti-Phospho-akt (SER473) (9277S, CellSignaling, 1:1000) and rabbit anti-cathepsin G (703590, Invitrogen, 1:1000), overnight at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... in 0.1% BSA in PBS for 1 hour before subsequent incubation with antirat Alexa Fluor 546 secondary antibody (1:400, Thermofisher, A-11081) for an additional hour ...
-
bioRxiv - Immunology 2023Quote: ... T cell activation was performed using plate-bound α-CD3 (clone 145-2C11, 1 μg/ml) and α-CD28 (clone PV-1, 1 μg/ml) monoclonal antibodies in RPMI 1640 medium (Life Technologies) supplemented with 10% FCS ...
-
bioRxiv - Physiology 2023Quote: ... the medium was changed to maintenance medium (DMEM high glucose #1-26P02-K, M199 EBS #1-21F01-I; both from BioConcept, 1% horse serum (#16050130; Thermo Fisher Scientific), 2% l-glutamine (#25030149 ...
-
bioRxiv - Neuroscience 2023Quote: ... anti-IBA1 (Wako, 019-19741, 1:200), anti-AT180 TAU (Thermo, MN1040, 1:100) and anti-AT8 TAU (Invitrogen, MN1020, 1:100) while muscle sections were incubated with anti-laminin (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... Invitrogen, 0.5 µL 12.5 mM dNTP mix, 1 µL 100 mM DTT, 1 µL SuperScriptIII reverse transcriptase, Invitrogen, 1 µL Ribolock inhibitor) was added and incubated (1 h ...
-
bioRxiv - Genetics 2023Quote: ... ADH enzyme activity (units: ΔAbs340nm min−1 mL−1) and total protein (units: mg mL−1; Pierce BCA Assay, Thermo Fisher Scientific) of each sample were measured 3 times in technical replicates ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... into the packaging 293T cells (with molar ratio for Luc-T20: N: M/E: S as 3: 1: 1: 1) with LipofectamineTM 2000 (Thermo Fisher Scientific). At 24 and 48 hours post-transfection ...
-
bioRxiv - Physiology 2023Quote: ... Cells were grown on DMEM/Hams F12 with 2% fetal bovine serum and standard cell culture hormones (Dexamethasone 1 1 mM; mM, T3 1 μM, ITS 100X Gibco cat # 41400045) on 6-well ...