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Citations for Thermo Fisher :
7301 - 7350 of 10000+ citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... coverslips were extensively washed with PBS and incubated for 2 hours at room temperature in the appropriate fluorophore-conjugated secondary antibody solution [Alexa Fluor 488- or Alexa Fluor 594-conjugated secondary antibody (Molecular Probes, Thermo Fisher Scientific) in blocking solution] ...
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bioRxiv - Cancer Biology 2019Quote: ... the sections were incubated overnight at room temperature with the indicated primary antibody prepared in blocking/permeabilization solution: mouse monoclonal antibody against HK-2 (1:100; Thermo Fisher, MA5-15679) or mouse monoclonal antibody against GLUT-3 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... Protein bands were detected using a primary antibody followed by an HRP-conjugated secondary antibody (rabbit, Life technologies #G21234, or mouse, Invitrogen #G21040) and visualized via chemiluminescence using Clarity Western ECL Substrate and the ChemiDoc MP Imaging System (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... The secondary antibodies used were Alexa Fluor® 488 or 594-conjugated goat anti-rabbit or anti-mouse IgG antibodies (1:300, Invitrogen, CA, USA) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-rabbit IgG secondary antibody conjugated to Alexa Fluor 568 and goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 568 (Invitrogen, dilution 1:500). Overall ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alexa Fluor 594 and 568-conjugated goat anti-rabbit IgG secondary antibodies and Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody were purchased from Invitrogen (Carlsbad, CA, USA), while Alexa Fluor 647-conjugated donkey anti-goat IgG secondary antibody was purchased from Abcam (Cambridge ...
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bioRxiv - Microbiology 2021Quote: ... the parasite mitochondrion was visualized using the Novus anti-HSP60 antibody and AlexaFluor 647-conjugated goat anti-rabbit 2° antibody (Invitrogen Life Technologies A21244), and mACP-HA2 was visualized with the rat anti-HA 3F10 primary antibody and FITC-conjugated donkey anti-rat 2° antibody (Invitrogen Life Technologies A18746) ...
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bioRxiv - Microbiology 2020Quote: ... Goat anti-mouse antibody conjugated with alexa fluorophore 647 and goat anti-rabbit antibody conjugated with alexa fluore 488 (Invitrogen, Inc. CA, USA) were used for immuno-fluorescence (IFA ...
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bioRxiv - Cell Biology 2021Quote: ... cells were permeabilised with phosphate buffer for 2 hours and then incubated with self-made anti-HA antibody (1:500) followed by Alexa488-conjugated secondary antibody (1:2,000) (Life Technologies, Thermo Fisher Scientific A11008). Specimens were mounted with Roti®-Mount FluorCare DAPI (Carl Roth ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were incubated with rabbit anti-tyrosine hydroxylase antibodies (TH; AB152, MilliporeSigma, MO) at 4°C overnight and then with fluorescent secondary antibodies (A-11012, Thermo Fisher Scientific, MA) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were then incubated with primary antibodies overnight at 4°C and then the secondary antibody conjugated with Alexa Fluor 488 (green) (Thermo Fisher Scientific, 35502) or Alexa Flour 594 (red ...
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bioRxiv - Microbiology 2022Quote: ... Coverslips were rigorously washed three times in PBST to remove unbound antibody and were subsequently incubated in AlexaFluor 488-conjugated secondary antibody (Life Technologies, 1:1000) in blocking buffer for 1 h at room temperature ...
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bioRxiv - Genetics 2022Quote: ... stained with antibodies to detect Arl13 (ciliary marker; ProteinTech cat. no. 17711-1-AP; secondary antibody: Invitrogen A27034 labeled with Alexa Fluor 488), γ-tubulin (ciliary basal body marker ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunolabeling with performed with mouse anti-B23 primary antibody (1:300; 2h) then detected with Alexa 488 donkey anti-mouse antibody (Invitrogen; 1:500; 1h), then further fixed with 4% paraformaldehyde in DEPC-PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... the secondary antibody (1/400 in blocking buffer, goat anti-rabbit Alexa 594 antibody, Thermo Fisher, A-11037 or donkey anti-mouse Alexa 488 antibody, Thermo Fisher, R37114) was added in blocking buffer for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibody incubation was carried out at 4°C overnight with a rabbit polyclonal antibody to Creb5 (PA5-65593, Thermo Fisher, 1:100), a mouse monoclonal antibody to Prg4 (MABT401 ...
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bioRxiv - Plant Biology 2021Quote: ... for use in experiments with anti-HA and anti-catalase antibodies as the primary antibodies and 1:1000 Alexa Fluor™ 488 goat anti-mouse IgG (H+L) (Thermo Fisher Scientific) for use in experiments with the anti-α-tubulin antibody as the primary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... After five times washing cycles with Wash Solution 2 for 30 min at RT the secondary antibody (1:200 in Antibody Diluent, goat anti-rabbit Alexa Fluor 568; Molecular Probes, Eugene, USA) was applied for 24 h (4 °C) ...
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bioRxiv - Microbiology 2020Quote: ... After sodiumcitrate pressure cooking, the rabbit anti-nucleocapsid antibody (NovusBio, code: NB100-56683SS, dilution 1:100) and an AlexaFluor-488 secondary antibody (ThermoFisher, code: A-11034) as well as the ProLong™ Gold Antifade Mountant with DAPI (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... with the appropriate secondary antibodies and the same anti-MCL-1 primary antibody (1:1000) and Clean-Blot™ IP Detection Reagent (HRP) (Thermo Scientific #21230) was used as secondary antibody (1:1000).
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bioRxiv - Developmental Biology 2022Quote: ... The primary antibodies used in this study were listed in Table S1 and secondary antibodies fused to Alexa fluorophore (Molecular Probes, 1:500). All stained sections were analysed with Zeis Imager Z2 (Zeis ...
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bioRxiv - Neuroscience 2020Quote: ... GFP was amplified with an anti-chicken GFP antibody from Abcam and retina slices were incubated overnight with primary antibodies in blocking solution followed by washes in PBS and a 3-hour incubation with anti-isotypic secondary antibodies (Invitrogen or Jackson Immunoresearch). Labeling of Dact2-GFP or Plcxd2-GFP retinas for GAD67 (mouse monoclonal ...
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bioRxiv - Genomics 2019Quote: ... Membrane was probed using GATA1 antibody (1:1000, rabbit, Cell Signalling Technologies cat no. 3535S) and GAPDH antibody (1:2000, mouse, ThermoFisher cat no. AM4300) as primary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... Vglut2a:DsRed] larval brains were stained using activated caspase-3 antibody followed by anti-rabbit IgG Cy5 conjugated antibody (Molecular Probes, A-10523,1:300) and imaged as previously described ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were then incubated overnight at 4 °C in 1:100 (in antibody diluent) anti-cadherin-11 antibody (CDH-11, rabbit polyclonal, Thermo Fisher, 71-7600). Cells were washed three times with PBS-tween and incubated in the conjugated secondary antibody ...
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A rare variant on a common risk haplotype of HFE causes increased risk of hereditary hemochromatosisbioRxiv - Genetics 2019Quote: ... then Western blots were performed using the anti-GFP antibody (1:2500) or a mouse anti-TfR1 antibody (ThermoFisher #13-6800, 1:500) or a secondary anti-rabbit HRP (Promega #W4011 ...
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bioRxiv - Cell Biology 2019Quote: ... and then specific antibodies were added overnight at 4°C prior to 30 min of secondary antibody (AlexaFluor488 conjugated; Molecular Probes, 1:1000) and DAPI staining ...
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bioRxiv - Biochemistry 2019Quote: ... Sections were washed 3x in 1x PBS and bound primary antibodies were detected by incubation with Alexa-fluor conjugated secondary antibodies diluted in blocking buffer (Thermo Fisher/Invitrogen, Eugene, OR) for 1 hr at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed in PBS and incubated with the appropriate AlexaFluor-564 conjugated antibody and AlexaFluor-488 conjugated secondary antibody (Molecular Probes; 1:200) as well as with Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Membranes with liver and muscle mitochondrial protein samples were incubated overnight with COX4 antibody (1:1.000 COX4 Polyclonal Antibody, cat no. PA5-17511, Thermo Fisher Scientific, Rockford, USA) and loading control protein ATP5A antibody (1:1.000 anti-ATP5A antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then probed with anti-MyHC antibody (M4276) followed by labeling with secondary anti-mouse antibody Alexa Fluor 488 (A31627, Invitrogen, Carlsbad, CA, USA). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... secondary antibody incubation was carried out using a 1:1000 dilution of Alexa 647 conjugated donkey anti-goat antibody (Life Technologies, Carlsbad, CA) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated with primary antibodies (anti-phos-CD31, anti-CD31, and anti-mCRP) and subsequently stained with secondary antibodies (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Cell Biology 2021Quote: ... sections were incubated with ACE2 and TTF-1 primary antibodies and the binding of the primary antibodies to their respective antigenic substrates was revealed by made-specific secondary antibodies conjugated with Alexa-488 (Life Technologies, 1:250) and Alexa-568 (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated sequentially with primary antibodies that detect either FLAG or phospho-Histone H2A.X (Ser139) and secondary antibodies (Alexa 488, Molecular Probes, Eugene, OR, US). Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’ ...
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bioRxiv - Immunology 2021Quote: ... The binding of the primary antibodies to their respective antigenic substrates was revealed by made-specific secondary antibodies conjugated with Alexa-488 (Life Technologies, 1:250) and Alexa-568 (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... including an incubation in the donkey anti-goat Alexa 488-conjugated secondary antibody as indicated above and then in a donkey anti-rabbit Alexa 546-conjugated secondary antibody (A10040, 1:500; Life Technologies, Madison, WI) overnight at 4°C.
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bioRxiv - Developmental Biology 2022Quote: ... Binding of the primary antibody was visualized with secondary donkey anti-rabbit antibody conjugated with AlexaFluor® 555 (1:1000, Molecular Probes, Invitrogen). UB branches and the collecting ducts were stained with FITC-conjugated DBA (1:500 ...
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bioRxiv - Neuroscience 2022Quote: Microglia activation was also assessed using the rabbit polyclonal Anti-Iba1 antibody (Wako Chemicals; 1:800) and the secondary antibody Donkey Anti-rabbit IgG conjugated with Alexa-594 (Thermo Fisher; 1:500), as previously described ...
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bioRxiv - Microbiology 2023Quote: ... the parasite mitochondrion was visualized using a polyclonal Rabbit anti-HSP60 antibody (Novus NBP2-12734) and AlexaFluor 647-conjugated Goat anti-Rabbit 2° antibody (Invitrogen Life Technologies A21244), the nucleus was stained with ProLong Gold Antifade Mountant with DAPI (Invitrogen Life Technologies P36931) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS three times and incubated with antibody solution with Alexa 568-conjugated anti-mouse IgG antibody (Molecular Probes, A-11019) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... bound antibody was detected by incubation for 1 h with secondary antibodies conjugated with Alexa Fluor 488 (1:200, Thermo Fisher Scientific, USA) or Alexa Fluor 546 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: Secondary antibodies: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (Alexa Fluor 488, Thermo Fisher Scientific, A-21202, 1:1000), Donkey anti-Mouse IgG (H+L ...
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Laser Scanning Confocal printing, a new simple method for micro-device fabrication (LSCprint method)bioRxiv - Bioengineering 2024Quote: ... After washing, incubation 30’ with secondary antibodies and DAPI (Termofisher, D1306) was performed: an Alexa 488 anti-mouse antibody (Thermo Fisher A-21202) was used to stain PH3-Ser 10 ...
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bioRxiv - Biochemistry 2024Quote: ... using a rabbit anti-WCTD antibody (GeneScript) (1:100) and Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary antibody (Invitrogen, Cat# A-31573) (1:500) ...
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bioRxiv - Neuroscience 2024Quote: ... incubated for 1.5 hours at room temperature with goat anti-rabbit HRP-conjugated antibody or donkey anti-chick HRP-conjugated antibody (Thermo Fisher Scientific, Massachusetts). Protein expression was detected by chemiluminescence using ECL (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... the sections were incubated first with anti-Ybx1 primary antibody and then Alexa Fluor 488/568-conjugated secondary antibody (Life Technologies, Carlsbad, CA). The sections were counterstained for nuclei with Hoecyst33342 ((Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed several times with PBS and subsequently incubated with secondary antibody with the following antibodies in blocking solution for 30-60 minutes: donkey anti-rabbit Alexa Fluor 488 (Themo Fisher Scientific; A-21206), donkey anti-mouse Alexa Fluor 555 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Next day sections were washed with 10% normal goat serum and incubated with appropriate secondary antibodies for 1 hour at room temperature in a humidified chamber and with a secondary antibody cocktail (Life Technologies, Waltham, MA) of Alexa Fluor 546 (1:250) ...
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bioRxiv - Molecular Biology 2023Quote: ... BMP6 was a generous gift from OSTEOGROW. ERFE was detected using DYKDDDDK Epitope Tag HRP-conjugated Antibody (R&D, Cat. # HAM85291) and FAM132B Polyclonal Antibody (Invitrogen, Cat. #PA5-67448). Recombinant ALK3-Fc fusion protein was purchased from R&D (Cat ...