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Citations for Thermo Fisher :
7251 - 7300 of 10000+ citations for Recombinant Rabbit CXCL12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected in a new tube and protein concentrations were measured using a BCA protein assay kit (Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: ... 600 μg of chromatin was precleared with 30 μl of Protein A- or Protein G-coupled magnetic beads (Invitrogen Dynabeads) for one hour at 4°C on a rotating platform ...
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bioRxiv - Plant Biology 2023Quote: ... Surface-exposed proteins were biotinylated and purified using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (ThermoFisher Scientific), following the manufacturer’s protocol with some modifications ...
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bioRxiv - Systems Biology 2023Quote: ... The protein concentration was determined with a BCA protein Assay kit following the instructions from the vendor (23225, Thermo Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged for 5 minutes at 16,000 x g and supernatant protein concentrations determined using the Pierce BCA Protein Assay Kit (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Bacterially expressed and purified NEMO proteins were labeled with the Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher, A30006) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 55,087 protein count) and contaminant protein database (provided by the manufacturer) in Proteome Discoverer software version 2.3 (Thermo Scientific, USA). A maximum of two trypsin cleavages were allowed with a precursor mass tolerance of 10 ppm and fragment mass tolerance of 0.8 Da ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant proteins were then quantified using the BCA assay and equivalent protein concentrations (800 µg) were immunoprecipitated with DynabeadsTM Protein G (Invitrogen) coupled to a mouse anti-biotin primary antibody at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The degree of labeling of the proteins with the fluorescent probes was evaluated by following the manufacturer’s protocol (Microscale Protein Labeling Kit, ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... The protein samples were resolved on 4-12% NuPAGE Bis-Tris gel with protein molecular weight standards (PageRuler Prestained Protein Ladder, 10 to 180 kDa, Thermofisher). The gels were transferred onto PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration in the lysates were determined by using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific #23225). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: To detect protein via western blotting 60µg of protein was resolved on SDS-PAGE 4- 12% Bis-Tris Bolt gels (Life Technologies). Protein was transferred onto a nitrocellulose membrane using a Mini Trans-Bolt Cell (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 μl were saved at −80ºC for posterior determination of protein content using Pierce BCA Protein Assay Kit (Cat. # 23227, Thermo Scientific). Samples were then immediately heat-inactivated and cooled down to room temperature.
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bioRxiv - Developmental Biology 2023Quote: ... The total protein concentration in the cell lysate was quantified by using BCA protein Assay kit (Thermo Scientific™, 23225).
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bioRxiv - Immunology 2023Quote: ... Protein concentrations were measured for each sample using the A280 NanoDrop method and a protein standard curve (ThermoFisher Cat. 23208). Afterwards ...
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bioRxiv - Immunology 2023Quote: Quantification of total protein levels from BAL fluid was measured using a BCA protein assay kit (Thermo Fisher Cat# 23227) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Total protein concentrations in membrane fractions were quantified using the Pierce™ BCA protein assay kit (ThermoFisher Scientific, Waltham, MA). For Western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... the protein concentration of the cell lysates was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific). Equal amounts of total protein (1 mg ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by electrophoresis at 16 mA followed by transferring the proteins to Immobilon-P membranes (Thermo Fisher Scientific) for 1 hour at 350 mA (4°C ...
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bioRxiv - Cancer Biology 2023Quote: Total protein was quantified using the Pierce bicinchoninic protein assay (BCA) kit in accordance with manufacturer’s instructions (23225, ThermoFisher Scientific). Following protein quantification ...
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bioRxiv - Neuroscience 2023Quote: ... transfected cell lysates (500 µg total protein in 200 µl) were pre-cleared by incubating with 10 µl Dynabeads protein G (Invitrogen) at 4°C for 1 hr ...
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bioRxiv - Immunology 2023Quote: ... 2% SDS and 10% Glycerol) and protein concentration was measured using Pierce™ BCA Protein Assay kit (Thermo-Fisher Scientific). 20µg of protein was resuspended with SDS-loading dye (50% β-Mercaptoethanol and 0.02% Bromphenolblue) ...
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bioRxiv - Developmental Biology 2023Quote: Membrane proteins were extracted from cell pellets using the Mem-PER™ Plus Membrane Protein Extraction Kit (89842, Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... and 10-15 µg of protein were loaded on 4-12% Bis-Tris Midi NuPage Protein Gels (Thermo Fisher Scientific) and separated at 120V for 2 h in MES running buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 14.000 RPM) and protein concentration of collected supernatants measured using the Pierce BCA Protein Assay Kit (#323227, Thermo Scientific, 23227). The samples adjusted to contain equal amounts of protein (10 μg or 12 μg ...
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bioRxiv - Physiology 2024Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The protein content of the samples was quantified using the Pierce™ 660 nm protein assay kit (Thermo Fisher, US) with BSA as the standard ...
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bioRxiv - Neuroscience 2023Quote: The protein concentration of the cell lysates was determined with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For co-immunoprecipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Biophysics 2023Quote: ... The final protein concentration was measured with the Micro BCA Protein Assay Kit (#OI191202, Thermo Fisher Scientific, Waltham, MA, USA). Protein purity was verified by SDS-PAGE and silver staining (Fig ...
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bioRxiv - Biophysics 2023Quote: ... the total protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce/Thermo Fisher Scientific; Rockford, IL). 25µg of protein samples were mixed with 4x LDS sample buffer (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Lysate inputs were normalized by determining overall protein concentration using Pierce™ BCA Protein Assay (Thermo Fisher Scientific, Waltham, MA). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cell Biology 2023Quote: Protein size separation of 25 µg of protein was done on a NuPAGE 4-12% Bis-Tris gel (Invitrogen, NP0336BOX) in NuPAGE MES SDS-buffer (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: We quantified Protein synthesis rate with the “Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit” (Thermo Fisher Scientific), with minor adaptations from the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Nascent protein synthesis was measured with the Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit (cat. no. C10428, ThermoFisher). Cells were plated in 12-well plates (Cell Treat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration of the CF was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration in the supernatant was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant proteins were then quantified using the BCA assay and equivalent protein concentrations (800 µg) were immunoprecipitated with DynabeadsTM Protein G (Invitrogen) coupled to a primary antibody at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were quantified via a BCA assay using the Pierce BCA Protein Assay Kit obtained from Life Technologies (Catalog #23225). Cell lysates were run through a 4-12% SDS-PAGE gel electrophoresis at 140-170V ...
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bioRxiv - Bioengineering 2023Quote: Concentrated EV or VLP total protein concentrations were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) These neutralization reagents were mixed with 4X loading dye ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble fraction was removed via centrifugation (13,000xg, 10 min, 4 °C) and protein abundance was quantified by BCA Protein Assay (Thermo Scientific). 50 μg of protein was utilized per well in a total volume of 60 μL ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 M sucrose) and a protein concentration assay was performed (BCA Protein Assay Kit, Pierce-Thermo Fisher Scientific, Melbourne, Australia). 100 μg of isolated mitochondria were collected and centrifuged at 12,000 g for 15 min at 4 °C and after removal of the supernatant the pellet was frozen at −80 °C for subsequent proteomics analysis.
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bioRxiv - Developmental Biology 2023Quote: ... Protein pellets were resuspended in 1% SDS and protein concentration was measured using the Bradford assay kit (Thermo Scientific, 1863028), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Protein concentration was measured with Pierce™ BCA Protein Assay Kit according to manufacturer’s guidelines (Thermo Fisher; Cat. No. 23225). Protein samples were mixed with 4X NuPAGE™ LDS Sample Buffer (Thermo Fisher ...
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bioRxiv - Physiology 2024Quote: ... The total islet protein content for each secretion assay was measured with Micro BCA™ Protein Assay Kit (ThermoFisher, #23235). Data represent the glucagon or insulin content per µg of protein in each tube.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the protein concentration in the supernatant was determined using a BCA Protein Assay Kit (Cat# 23225, Thermo Fisher Scientific). Afterward ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Between 20-50 μg of protein per sample was mixed with Laemmli sample buffer and boiled for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants (1.2 mL) were subjected to immunoprecipitation with 30 μG of protein G magnetic beads (Dynabeads Protein G, Thermo Scientific) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... The 20 μg of extracted proteins were quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, WA, USA) at 500 ng/μL ...