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Citations for Thermo Fisher :
7251 - 7300 of 7736 citations for Recombinant Human BLNK His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... 400 ng of the constructed plasmids containing the human gene transcriptional units (Table S9D) were linearized by digestion with NotI (FastDigest, Thermo Fisher Scientific) according to the manufacturer’s protocol for 30 min and subsequently the digestion mix was directly transformed to IMX1076 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Supernatant was harvested at the indicated timepoints and IL-2 levels in the supernatant were measured via IL-2 Human Instant ELISA kit (Thermo Fisher #BMS221INST). T-cell proliferation was also measured at the indicated timepoints using a BD FACSymphony Fortessa X-50.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Calu-3 (human, Caucasian, lung, adenocarcinoma) cell line was obtained from ATCC and maintained in Minimum Essential Medium (MEM; Gibco, Thermo-Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Human iPS cells were maintained and propagated as colonies in feeder-free conditions in Essential 8 Flex Medium (Thermo Fisher Scientific, A2858501) on Matrigel hES qualified (Corning ...
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bioRxiv - Microbiology 2021Quote: Characterization of the immune response via bead-based determination of cytokine and chemokine concentrations was performed using a Life Technologies Cytokine 37-plex Non-Human Primate Panel for Luminex platform (#EPX370-40045-901, Thermo Scientific, USA) according to manufacturer instructions in BSL-2+ conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed using TaqMan gene expression assays (Human-Assay ID Hs00917510_m1; and (Rat-Assay ID Rn01489483_m1; Applied Biosystems, Life Technologies, Carlsbad, CA). Gene expression was quantified by the comparative Ct value ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were pelleted and incubated with 100 μl of human AlexaFlour-488-conjugated antibody (1:200 in PBS/BSA; Thermo Fisher Scientific) and rotated again as described above ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... maintenance culture was carried out for all cell lines including human ES cells using albumin-free Essential 8 Medium (Thermo Fisher Scientific) in six-well feeder-free culture dishes coated with 5 μg/mL vitronectin (VTN-N ...
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bioRxiv - Cell Biology 2021Quote: ... A non-targeting sgRNA that does not recognize any sequence in the human genome was used as a negative control (Invitrogen Cat#: A35526). The deletion efficiency of HIF-1α and syndecan-1 proteins was measured by western blot and flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from 100 mg of human prefrontal tissues using the TRIzol reagent (Thermo Fisher Scientific, Inc. Waltham, MA, USA), as described by the manufacturer ...
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bioRxiv - Biophysics 2021Quote: ... After washing thrice with PBS-0.1% Tween 20 the membrane was probed with primary antibody which is a rabbit monoclonal anti human lamin A+C antibody (Thermo Scientific, USA) and Stabilized Peroxidase Conjugated secondary Goat Anti-Rabbit (H+L ...
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bioRxiv - Biophysics 2020Quote: ... while hNAA25 contained a Tobacco-etch virus (TEV)-cleavable N-terminal 6xHis-tag. Human NatB complex (hNAA201-163/hNAA25FL) was obtained by coexpressing these two plasmids in Sf9 (S. frugiperda) cells (ThermoFisher, cat# 12659017), and purified as described previously[35] ...
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bioRxiv - Biophysics 2020Quote: The plasmid pET3a containing human AβM42 and AβMC40 cDNA was transformed into Escherichia coli (E. coli) One Shot® BL21 (DE3) pLysS (Thermo Fisher Scientific, USA). The plasmids were a kind gift from Prof ...
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bioRxiv - Cancer Biology 2021Quote: ... and metastasis human colon cancer cell line (SW-620) was cultured in Roswell Park Memorial Institute medium 1640 (RPMI 1640: Gibco, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human AB serum (produced in house) and 100 U/mL penicillin and 100 µg/mL streptomycin (Thermo Fisher Scientific). Mixed PBMCs were plated at densities of 600,000 cells per 200 µL and per well of 96-well u-bottom shape plates (Corning ...
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bioRxiv - Cancer Biology 2022Quote: ... The blots were incubated separately either with mouse monoclonal anti-human CD44 (156-3C11) antibody (Cat# MA513890, ThermoFisher Scientific, Waltham, MA, USA), mouse monoclonal anti-human CD63 antibody (Cat# ab8219 ...
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bioRxiv - Genetics 2022Quote: BJ cells were transfected with 60nM of human siRNA for different timings (indicated in the main text for each experiment) using Lipofectamine RNAiMax reagent (Invitrogen, 13778-075) and Opti-MEM medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28, ThermoFisher Scientific) at a 1:5 ratio (bead:cell ...
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bioRxiv - Cancer Biology 2022Quote: Organoid cultures established from human ACCs were dissociated from Matrigel by incubation with a solution of 2 mg/mL Dispase-II (Thermo Fisher, 17105041) and 200 U/mL Collagenase-III (Worthington ...
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bioRxiv - Cell Biology 2022Quote: ... synthesized to knockdown human GJA1 mRNA (sequence 5’ to 3’: GGGAGAUGAGCAGUCUGCCUUUCGU; cat. # HSS178257) and Stealth™ RNAi (Thermo Fisher scientific, cat. # 12935112) was used as a negative control ...
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bioRxiv - Systems Biology 2022Quote: Adult human epidermal keratinocytes were cultured in EpiLife serum-free keratinocyte growth medium supplemented with Human Keratinocyte Growth Supplement (HKGS) and 100 units/mL Penicillin and 100 μg/mL Streptomycin (Thermo Fisher Scientific). Adult human dermal fibroblasts were cultured in Medium 106 supplemented with Low Serum Growth Supplement (LSGS ...
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bioRxiv - Neuroscience 2022Quote: The expression level of 752 miRNAs was screened by real-time PCR with TaqMan Advanced miRNA Human A and B Cards (Applied Biosystems A31805). cDNAs were diluted 1:10 with 0.1X TE buffer ...
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bioRxiv - Microbiology 2022Quote: ... ATCC TIB-202) or primary human monocytes (PHMs) were propagated in RPMI 1640 with L-glutamine and 25 mM HEPES buffer (Invitrogen, Carlsbad, CA), supplemented with 1 mM sodium pyruvate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Cell-free plasma was analyzed using a human magnetic cytokine panel providing simultaneous measurement of 35 cytokines (Thermo Fisher Scientific, Waltham, MA). The assay was performed according to the manufacturer’s instructions with each subject sample performed in duplicate and then analyzed on a Luminex FLEXMAP 3D instrument.
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bioRxiv - Bioengineering 2022Quote: ... WBCs were isolated from human whole blood and also stained with a live cell marker (10 μM CMTPX, Invitrogen™, Carlsbad, USA). Detailed methods for MCF-7 cell culture and staining of cancer cells and WBCs are provided in our previous works 24-26 ...
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bioRxiv - Bioengineering 2022Quote: ... Human bone marrow mesenchymal stem cells (hMSCs, RoosterBio, Maryland, USA) were cultured in alpha Minimal Eagle’s medium (α-MEM) (Gibco, Carlsbad, USA) with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: Male mouse neuroblastoma Neuro-2a (N2a; ATCC, CCL-131) and female human epithelial HeLa (ATCC, CCL-2) cells were maintained in MEM (Thermo Fisher Scientific), supplemented with 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed once and incubated with a PE-labelled goat anti-human IgG secondary antibody (Thermo Fisher Scientific 12-4998-92) at a final concentration of 2.5μg/mL of 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: Raw MS Data were searched against the human SwissProt protein database (Version November 2020) with Proteome Discoverer 2.4 (ThermoFisher Scientific, Bremen, Germany) using the following parameters ...
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bioRxiv - Developmental Biology 2022Quote: Culture media consisted of LWRN conditioned media generated as previously described45,46 and combined with human basal media (Advanced DMEM/F12 (Gibco, Cat#12634-028); Glutamax 4 mM (Gibco ...
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bioRxiv - Cell Biology 2022Quote: MRC5 cells (human lung fibroblasts, ATCC® Cat# CCL-171TM, RRID:CVCL 0440) were maintained in MEM media (Cat# 11095080, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... Immune mediator levels in COVID-19 patient plasma across different active and convalescent groups were measured with 24-plex Human ProcartaPlex™ (ThermoFisher Scientific). The kit analyte detection panel included brain-derived neurotrophic factor (BDNF) ...
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bioRxiv - Microbiology 2021Quote: ... plates were washed three times and incubated for 30 minutes at room temperature with cross-absorbed goat anti-human IgG-horseradish peroxidase (HRP)-conjugated secondary antibody (ThermoFisher Scientific; A18811) diluted to a 1:2500 dilution in ELISA wash buffer ...
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bioRxiv - Microbiology 2020Quote: ... containing 10 μg/ml DNase I (StemCell; #7469) and re-plated into 12-well plates coated with 10 μg/ml human bulk fibronectin (ThermoFisher Scientific; #3560) at a density of 750,000 cells/well ...
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bioRxiv - Biochemistry 2021Quote: Resting CD4+ T cells were purified from bulk PBMCs by using Dynabeads™ Untouched™ Human CD4 T Cells Kit (ThermoFisher Scientific). Selected CD4+ T cells were activated by Dynabeads™ Human T-Activator CD3/CD28 kit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and the corresponding light chain (human IgKappa, IgLambda) expression vectors respectively and produced in transiently expressed in Expi-CHO-S cells (Thermo Fisher, #A29133) at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: To facilitate the analyses of invasion assays, HeLa cells (human cervical carcinoma, ATCC® CCL-2™) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2020Quote: Human HGSNAT codon-optimized cDNA Tordo (31) fused to a GFP (HGSNAT-GFP) was cloned into a pENTR1A vector (Invitrogen, 11813-011) and then transferred to a 3rd generation lentiviral vector plasmid (pLenti PGK Blast DEST (w524-1) ...
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bioRxiv - Molecular Biology 2020Quote: ... Commercially available ELISAs for quantitative detection of human total IgG were used according to manufacturer’s protocols (Thermo Fisher Scientific, Waltham, MA, U.S.A.). All plates were read in a microtiter plate reader at 450 nm and optical density values were used in statistical analyses.
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bioRxiv - Microbiology 2021Quote: ... followed by one wash in a large volume of PBS and then incubation with Alexa647-coupled anti-human secondary antibody (Thermo Fisher Scientific) at 1:200 in 10% FCS in PBS ...
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bioRxiv - Biochemistry 2020Quote: hTERT keratinocytes were cultured with Epilife media supplemented with human Keratinocyte Growth Supplement and Gentamicin/Amphotericin B 500X (Thermo Fisher Scientific, USA). Cells grown to passage 3 were treated for 24 hr with vehicle (DMSO ...
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bioRxiv - Microbiology 2020Quote: ... They were then incubated with 1:200 diluted Alexa Fluor 594 conjugated goat anti-human IgG secondary antibody (Invitrogen, Thermo Fisher Scientific) for 45 min at RT and subjected to flow cytometric analysis with a CytoFLEX flow cytometer (Beckman) ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was extracted and the whole transcriptome of ALDH+ and ALDH-populations was assessed using Affymetrix Whole-Transcript Human Gene 1.0 ST Array (Affymetrix, Thermo Fisher Scientific) (30) ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Biochemistry 2021Quote: ... and the corresponding light chain (human IgKappa, IgLambda) expression vectors respectively and produced in transiently transfected ExpiCHO-S cells (Thermo Fisher, #A29133) at 37°C and 8% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... Recovered nanovials were washed two times with washing buffer and sequentially labeled with streptavidin and biotin goat anti-human IgG Fc (Thermo Fisher, A18821) following standard procedures described above ...
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bioRxiv - Cancer Biology 2021Quote: ... together with 0.5µg of a plasmid expressing the renilla luciferase under the control of the human β-actin promoter using Lipofectamine 3000 (ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Human VAT and SAT biopsies (30-50g) were minced and digested with 3mg/mL type II collagenase solution (ThermoFisher Scientific Inc./Gibco) in HBSS containing calcium chloride ...
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bioRxiv - Microbiology 2022Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37° C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
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bioRxiv - Microbiology 2022Quote: His-tagged soluble human ACE2 was produced in FreeStyle™ 293-F cells by transfecting soluble human ACE2 (1-741 aa) expression vector plasmid DNA using 293fectin (Thermo Fisher) and purified using HiTrap Chelating column charged with nickel (GE healthcare ...