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Citations for Thermo Fisher :
7251 - 7300 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was quantified using a PierceTM BCA Protein Assay (ThermoFisher Scientific, 237227) and frozen at -80°C until downstream analysis.
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bioRxiv - Neuroscience 2024Quote: ... protein concentration was quantified using a PierceTM BCA Protein Assay (ThermoFisher Scientific, 237227) and frozen at -80°C until downstream analysis.
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were measured using a BCA Protein Assay Kit (Thermo Fisher Scientific), alongside a BSA protein standard (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... total protein concentration was quantified using BCA Protein assay (Thermo Fisher Scientific, Inc.). A total of 30 µg of proteins in breastmilk samples were separated on 8 or 10% Tris-Glycine gels (Titan Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), and 10 μg electrophoresed in 4–12% Bis-Tris denaturing gels (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific), and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured using the detergent-compatible (BCA) protein assay (Thermo Scientific). Denatured lysates were separated on NuPage 8-16% Tris-glycine Midi Protein gels (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Thermofisher Scientific) and normalized to 1 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Bulk proteins were visualized with the No-Stain Protein Labeling Reagent (#A44717, ThermoFisher) and imaged using the iBright Imaging System (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was quantified using the Micro BCA Protein Assay Kit (Thermo Fisher Scientific). Lysates were subjected to SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225), and 30 µg of the lysates were collected as input ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225), and 30 µg of lysate was collected as input ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) and 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with anti-HA antibody (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantification of protein concentration was processed using the BCA protein assay (Thermo fisher). A total protein at concentration per lane at 50 μg was loaded into the 10% SDS-PAGE and then transferred to PVDF membranes ...
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bioRxiv - Molecular Biology 2020Quote: Blood samples from the discovery cohort were collected by cardiac puncture at euthanasia and 50 µl of whole blood was stored in Lysis/Binding solution (MagMax 96 blood RNA isolation kit, Thermo Fisher, USA) at -20 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and the top six scFvs displaying the highest binding signals were reformatted into the IgG format and expressed in Expi293™ cells (Thermo Fisher) and subsequently purified using an Äkta Pure system (GE Healthcare ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA concentrations were estimated using a fluorimeter and fluorescent DNA-binding dye (Qubit™ dsDNA BR Assay Kit; Thermo Fisher Scientific, USA), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The level of antibody binding was measured as optical density at 450nm (for TMB) using the Multiskan Go plate reader (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... 50 μl serially diluted pMHC were added to each well of high-binding capacity streptavidin-coated 96-well plates (#15500; Thermo Fisher Scientific). After a minimum 45-min incubation at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... washed with cold PBS and resuspended in 100 µl of cold 1X Annexin-binding buffer supplemented with AlexaFluor 488 annexin V (Thermo Fisher Scientific) and incubated in the dark for 15 minutes ...
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bioRxiv - Genomics 2019Quote: ... Adaptor ligated nascent RNA was enriched with biotin-labeled products by another round of Streptavidin bead binding and washing (two washes each of High, Binding and Low salt buffers and one wash of 1x SuperScript IV Buffer [Thermo Fisher Scientific]), and reverse transcribed using 25 pmol RT primer (5’-AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGA-3’ ...
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bioRxiv - Plant Biology 2019Quote: ... positive genomic regions for PHE1 binding containing SNPs that allowed distinction between parents were amplified by PCR using the Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific), in combination with the primers described above ...
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bioRxiv - Immunology 2021Quote: ... Columns were washed twice with gentle Ag-Ab binding buffer prior to elution in 100-200 μL gentle Ag-Ab elution buffer (Thermo Fisher Scientific). Eluted VLP-CH01 mixtures were then buffer exchanged into 100μL 50mM Tris/HCl pH 8.0 for subsequent reduction and alkylation.
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bioRxiv - Immunology 2021Quote: ... Screw cap spin columns were incubated with protein A–agarose for 10 minutes to allow for spin column resin equilibration before washing with gentle Ag-Ab binding buffer (Thermo Fisher Scientific). VLP-CH01 complexes were then applied to the spin columns and left to incubate for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: The atoxic binding fragment of tetanus neurotoxin (HCT) was bacterially expressed and labelled with AlexaFluor647 antibody labelling kit (Life Technologies, A-20186) as previously outlined (Gibbs et al. ...
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bioRxiv - Microbiology 2021Quote: We detected fungal cells with compromised cell membranes by recording the fluorescence of the DNA-binding dye SYTOX green (Molecular Probes, USA). Permeabilization of the fungal membrane allows the dye to cross the membranes and to intercalate into the DNA ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell suspension (100 μl per well-per single aggregate) was transferred to low binding 96-well plate (Nunclon Sphera U-Shaped Bottom Microplate, Thermo Fisher Scientific) and centrifugated (for 3 min at 180 × g ...
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bioRxiv - Developmental Biology 2021Quote: ... Organoids were imaged from day 1 to day 2 at room temperature directly in low binding 96-well plate (Nunclon Sphera U-Shaped Bottom Microplate, Thermo Fisher Scientific). The volume of the organoids was acquired with 2.24 µm z step size using 488 nm excitation laser at 10% intensity in 30 min intervals.
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bioRxiv - Microbiology 2020Quote: ... SIC1.301 and SIC1.300 or SIC fragments 1,2 and 3 and binding was detected using 1 in 25,000 dilution of HRP-conjugated goat anti-rabbit IgG (Life Technologies, Paisley, UK). To determine detection of SIC fragments by human purified anti-SIC antibody or antenatal serum ...
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bioRxiv - Pathology 2021Quote: ... 90 µl blood was stabilized with 200 µl mixture of lysis/binding solution concentrate and isopropanol (MagMax 96 blood RNA isolation kit, Thermofisher, Roskilde, Denmark), and stored at -80°C for later RNA extraction ...
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bioRxiv - Genetics 2020Quote: ... Cell lysates (250-300 µg) in 0.3 ml HEPES binding buffer were incubated with 20 µl Pierce™ anti-c-Myc magnetic beads (Thermo Fisher Scientific) for 3hr at 4°C with mixing ...
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bioRxiv - Pathology 2020Quote: ... were also mixed with 520 μl mixture of lysis/binding solution concentrate and isopropanol (MagMax 96 blood RNA isolation kit, Thermofisher, Roskilde, Denmark), and stored at −80°C until RNA extraction for transcriptomics and qPCR analysis.
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bioRxiv - Cell Biology 2021Quote: ... To visualize antibody binding the following secondary antibodies were used: goat anti-mouse Alexa Fluor 488 (2 μg/ml, catalog number A11029, Thermo Fisher Scientific) and goat anti-rabbit Alexa Fluor 546 (2 μg/ml catalog number A11035 ...
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bioRxiv - Cancer Biology 2021Quote: ... we cloned oligonucleotides corresponding to the region of the 3’UTR containing each miRNA binding site (plus 30bp upstream and downstream) downstream of the luciferase reporter in the pMIR-REPORT vector (Life Technologies #AM5795M) using restriction enzymes ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then resuspended in annexin-binding buffer and stained with Alexa Fluor 488 conjugated annexin V (Thermo Fisher, catalog# A13201) and 1 µg/ml Propidium iodide (PI ...
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bioRxiv - Immunology 2020Quote: ... DOPC and DOPS liposomes prepared in TBS were immobilized at 10 µg/ml in binding buffer (TBS, 0.1% BSA) on streptavidin-coated high capacity plates (Thermo Fisher, Waltham, MA). Increasing concentrations (0.01-10 µg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... A DNA fragment encoding the SacB ORF including the Pcat promoter and the ribosome binding site from bacteriophage T7 gene 10 (Olins & Rangwala, 1989) and was created by gene synthesis (GeneArt, Thermo Fisher Scientific). pGRNA-galK was linearized by PCR using primers 95 and 391 and assembled with the sacB-fragment by Gibson assembly (New England Biolabs).
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bioRxiv - Systems Biology 2022Quote: ... Approximately 50 mg tissue was homogenized in 0.8 ml Lysis/Binding Buffer from mirVana miRNA Isolation Kit (Thermo Fisher, Grand Island, NY) by using FastPrep-24 5G homogenizer (MP Biomedicals ...
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bioRxiv - Neuroscience 2022Quote: ... and their CA1 region bolus-stained with the Na+ sensitive dye SBFI-AM (sodium-binding benzofuran isophthalate-acetoxymethyl ester; Invitrogen, Karlsruhe, Germany). SBFI was alternatively excited at 340/380 nm at an imaging frequency of 0.5 Hz and emission was collected >440 nm from defined regions of interest (ROIs ...
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bioRxiv - Molecular Biology 2022Quote: All native binding experiments were performed using a Q Exactive Orbitrap MS with Ultra High Mass Range (UHMR) platform (Thermo Fisher Scientific) [80] ...
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bioRxiv - Cell Biology 2024Quote: ... Immediately prior to loading into the flow cytometer the supernatant was carefully aspirated and cells resuspended in 200 µL of binding buffer containing 500 nM Sytox Blue (ThermoFisher Scientific (Invitrogen)) and analyzed by BD Fortessa cytometer (BD Biosciences) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein bound to the quartz membrane was washed with 150 µL binding buffer three times and digested with Pierce™ Trypsin Protease MS-Grade (Thermo Scientific) dissolved in 50 mM TEAB buffer with digestion ratio of 1:20 ...
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bioRxiv - Biochemistry 2024Quote: RNA oligonucleotides used for binding affinity measurements and crystallographic studies were synthesized on an ABI 394 DNA/RNA synthesizer (Applied Biosystems (ABI); Waltham ...
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bioRxiv - Microbiology 2024Quote: Primers outflanking crRNA binding sites were used to amplify APT coding sequences using Phire Plant Direct PCR Master Mix (Thermo Fisher, NL) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed 3 times with TBS-T and phage binding was detected by addition of 20 µl SuperSignal™ ELISA Pico Chemiluminescent Substrate (Thermo-Fisher Scientific) and read on an Envision multilabel plate reader (Perkin-Elmer) ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction mixture was left in a heatblock at 22 °C for 5 min before sampling every 30 s into a chilled quenching buffer (binding buffer from Thermo Scientific, K0701). To blunt the digested DNA ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells transfected with WT or mutant GPR40 plasmids for IP1 assay and or Binding Assay were harvested with TrypLE (Thermo Fisher Scientific), resuspended in optiMEM (Life Technologies ...
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bioRxiv - Genomics 2023Quote: Levels of H3K27ac CUT&Tag binding signal was determined by qPCR amplification carried out with the QuantStudio™ 5 Real-Time PCR System (ThermoFisher A34322) using the Standard Curve experiment type and SYBR Green Master Mix (ThermoFisher 4309155) ...
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bioRxiv - Plant Biology 2023Quote: ... while the AtURI CDS was transferred into pGBKT7-GW to generate a GAL4 DNA binding domain fusion by Gateway (Thermo Fisher Scientific). pGADT7- and pGBK7-derived expression vectors were introduced into Y187 and Y2HGold yeast strains ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria from day two plates were resuspended in PBS and adjusted to an OD 600 nm of 0.5 to coat high binding ELISA plates (Nunc-Immuno™, ThermoScientific). After incubation overnight at 4ºC ...