Labshake search
Citations for Thermo Fisher :
7201 - 7250 of 10000+ citations for Recombinant Human ITGAV & ITGB5 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... 1 µL of the reverse transcription reaction was used to amplify regions of interest using gene-specific primers in a 50 µL PCR reaction using recombinant Taq DNA Polymerase (Invitrogen) (see Table S2) ...
-
bioRxiv - Biochemistry 2023Quote: ... The resulting plasmid was utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen). Exponentially growing Sf9 cells (2 x 106 cells/mL in Lonza Insect-XPRESS medium supplemented with 0.1 mM biotin ...
-
bioRxiv - Biophysics 2023Quote: ... These plasmids were used for the generation of recombinant baculoviruses following Bac-to-Bac expression system protocols (Invitrogen, Catalog # 10359016).
-
bioRxiv - Neuroscience 2023Quote: ... Each tissue was homogenized in 500 μL of lysis buffer containing 400 units of RNase inhibitor (RNaseOUT Recombinant Ribonuclease Inhibitor, 10777-019, Invitrogen) and 0.1% Triton-X100 in low sucrose buffer (0.32 M sucrose ...
-
bioRxiv - Plant Biology 2024Quote: ... The recombinant plasmid and the empty pBI121 vector underwent digestion using the restriction enzymes XbaI and SacI (Thermo Scientific TM). The purified digested insert and vector ligated using T4 DNA ligase at a 3:1 ratio ...
-
bioRxiv - Microbiology 2023Quote: Recombinant baculoviruses (AcNPV) were generated using pFastBac1 AcGFP-JEVcore-FOS and a Bac-to-Bac system (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA was produced using random hexamer primers (Mycrosynth) with recombinant M-MuLV reverse transcriptase (RevertAid, Thermo-Fisher Scientific, Cat #K1691) and used for quantitation of TTP-bound RNA and total RNA by RT-qPCR with HOT FIREPol EvaGreen qPCR Supermix (Medibena ...
-
bioRxiv - Microbiology 2024Quote: ... 1μg recombinant PolyHis-p62 was adsorbed to 5 μl pre-equilibrated packed Ni-NTA resin (Thermo Fisher Scientific, Cat# 88221) via 1 hr ...
-
bioRxiv - Immunology 2024Quote: ... or 50 ml of 0.5 mg/ml MSP2 recombinant antigen (McCarthy et al., 2011a) in PBS were coated to 96-well flat bottom MaxiSorb plates (Nunc) overnight at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... and monoclonal mouse IgG α-PAN-pLDH (PA-2, AccessBio, Somerset, NJ) were biotinylated using the EZ-Link Sulfo-NHS-Biotin Kit (21435, Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions with minor modifications (See Additional file 1 ...
-
bioRxiv - Immunology 2024Quote: ... The native or chimeric 8H11 mAbs were biotinylated in a 20-fold molar excess of EZ-link NHS-PEG4-Biotin (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s recommendations ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: We used a biotinylated AB from Jackson Immuno Research (711-065-152, anti-rabbit) and the respective streptavidine conjugates from ThermoFisher (S21381, Alexa FluorTM 555 conjugate) and (S11223 ...
-
bioRxiv - Immunology 2021Quote: ... at 4°C with 2µg anti-CD3 (mouse: eBioscience 14-0031-86; human: eBioscience 16-0037-85) and 1µg anti-CD28 (mouse: Invitrogen 14-0281-86; human: 16-0289-85) and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
-
bioRxiv - Molecular Biology 2020Quote: ... Supernatants were then transferred into fresh Eppendorfs and frozen at −80°C (for radiolabeled glucose to protein) or their protein concentrations calculated (Western blot) using a Pierce BCA Protein Assay kit (Thermo Scientific, Cat#23225, Waltham, MA, USA).
-
bioRxiv - Neuroscience 2023Quote: ... pellets were reconstituted in 50[µl of 0.1[N NaOH and kept for protein quantification using a BCA protein assay kit (Thermo Scientific Pierce BCA Protein Assay Kit, PI23227). Dialysate concentrations are expressed as ng/ml and tissular concentrations as ng/mg of total proteins.
-
bioRxiv - Neuroscience 2023Quote: Total protein levels in cortex homogenates were quantified using a Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: ... Protein amounts were determined using a Qubit4 Fluorometer and Qubit Protein Assay kit (Thermo Fisher Scientific). GUS activity was calculated in (nmol 4-MU ...
-
bioRxiv - Microbiology 2020Quote: ... Lysate protein concentrations were determined using a BCA protein assay kit according to manufacturer’s instructions (ThermoFisher).
-
bioRxiv - Neuroscience 2021Quote: ... The supernatant was collected for protein quantification using Pierce™ BCA Protein Assay Kit (Thermo Scientific).
-
bioRxiv - Developmental Biology 2020Quote: Changes in protein expression were monitored using Click-iT Protein Synthesis Assay kit (Thermo Fisher Scientific). Oocytes and 2 cell embryos were cultured in M16 medium with the methionine analogue HPG at a dilution of 1:500 ...
-
bioRxiv - Cell Biology 2020Quote: ... and DNA-protein complexes were pulled down using Protein A/G agarose beads (Thermo Fisher, 20423). Agarose beads were washed with the following buffers ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined in the supernatant by BCA Protein Assay Kit (Thermo Fisher Scientific, #23227). 100 μg of protein was transferred into a new tube and the final volume was adjusted to 100 μL with 100 mM triethylammonium bicarbonate (TEAB) ...
-
bioRxiv - Cell Biology 2020Quote: ... the supernatants were collected for measuring the protein concentration with BCA Protein Assay Kit (ThermoFisher Scientific). Cytosolic and nuclear extracts were prepared using NE-PER reagents (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... Total protein concentration of the supernatant measured using the Pierce BCA protein assay kit (Thermo Scientific) was used as a control.
-
bioRxiv - Immunology 2021Quote: ... and sample protein concentration determined using the Pierce® BCA Protein Assay Kit (Thermo scientific, UK). SDS-PAGE was then performed ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were mixed with 20 μL Protein G-conjugated magnetic beads (DB10009, Thermo Fisher Scientific) with 2.0 μg antibody ...
-
bioRxiv - Microbiology 2019Quote: ... Protein concentration for each sample was determined using the Pierce BCA Protein Assay kit (Thermo Scientific).
-
bioRxiv - Molecular Biology 2021Quote: ... Supernatants were collected and protein quantification was performed using a BCA protein assay kit (Thermo Scientific). A total of 2,000 μg of protein extract was used to perform the immunoprecipitation ...
-
bioRxiv - Molecular Biology 2021Quote: ... The extracted proteins were quantified by Pierce™ Modified Lowry Protein Assay Kit (Thermo Fisher Scientific) prior to precipitation by the phenol/chloroform method (Wessel and Flügge 1984) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Lysate total protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific, 23225).
-
bioRxiv - Evolutionary Biology 2021Quote: ... Total protein extracts were prepared by using B-PER® Bacterial Protein Extraction Reagent (Thermo Scientific) as per manual ...
-
bioRxiv - Neuroscience 2020Quote: ... The protein concentration of pooled triplicates was determined using the BCA protein assay (Thermo Fisher Scientific) and 8 μg of total protein subjected to immunoblotting against cleaved caspase-3 and −7 (1:200 dilution ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific, Rockford, IL, USA). Samples were prepared for SDS-PAGE in Laemmli sample buffer (Bio-Rad ...
-
bioRxiv - Microbiology 2022Quote: ... The protein concentration was determined with the BCA Protein Assay Kit (Thermo Fisher Scientific, Shanghai, China).
-
bioRxiv - Cell Biology 2022Quote: ... Supernatants were assessed for total protein using the BCA protein quantitation kit (Thermo Fisher Scientific, UK). 15 μg of protein were separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
-
bioRxiv - Biophysics 2022Quote: Oocyte surface proteins were purified using the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific), modified here for oocytes as previously described (Lynagh ...
-
bioRxiv - Systems Biology 2022Quote: ... Protein concentration was measured by Pierce™ BCA Protein Assay Kit (Thermo Fisher cat. no. 23225). 20 ug of total protein from each lysate was loaded and separated on a NuPAGE 4%–12% Bis-Tris Gel (Invitrogen cat ...
-
bioRxiv - Neuroscience 2021Quote: ... and total protein concentration determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific 23225), as described above for immunoblotting ...
-
Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... 10 μg of protein lysate was mixed with 4x protein sample buffer (Invitrogen or Bio-Rad) and water or lysis buffer to a volume of 15 μL ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the Qubit® Protein Assay Kit (Thermo Scientific Rockford, IL, USA). For both cases ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein and RNA concentration were measured using 660nm Protein Assay Reagent (Thermo Fisher Scientific, 22660) with Ionic Detergent Compatibility Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... total protein on the membrane was visualized using No-Stain Protein Labeling Reagent (Thermo Fisher Scientific). The blot was immersed in the primary antibody overnight at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The co-purified proteins were eluted by boiling in 1x NuPage Protein Loading buffer (Thermo Fisher) supplemented with 5% Beta-mercaptoethanol ...
-
bioRxiv - Molecular Biology 2020Quote: ... The concentration of purified proteins were measured by Pierce BCA protein assay kit (Thermo Fisher Scientific). The purified proteins were stored at −80°C until further use.
-
bioRxiv - Neuroscience 2021Quote: ... and the protein concentration was determined using a Pierce BCA Protein Assay Kit (Thermo Scientific #23227). 20μg of protein were denatured in LDS (4X Bolt™ LDS Sample Buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein concentration was measured by the BCA protein assay kit (Thermo Fisher, Waltham, MA, USA). The SDS protein sample buffer (MilliporeSigma ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 40 μg of protein was loaded onto NuPAGE Bis-Tris 10% Protein Gels (ThermoFisher #NP0301BOX), and proteins were transferred to nitrocellulose membranes (Sigma #GE10600002) ...