Labshake search
Citations for Thermo Fisher :
7201 - 7250 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... 1% penicillin/streptomycin (Gibco), 10ug/ml blasticidin ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1% penicillin/streptomycin (Gibco), and 10ng/ml IL-3 (Fisher) ...
-
bioRxiv - Neuroscience 2024Quote: ... 1% NEAA (ThermoFisher Scientific), 1% CD lipids (ThermoFisher Scientific) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1% Glutamax (Gibco 35050061), and 1% Penicillin-Streptomycin (Gibco 15140122) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1% Glutamax (Gibco 35050061), 1% MEM Non-Essential Amino Acids (Gibco 11140050) ...
-
bioRxiv - Neuroscience 2024Quote: ... 1% GlutaMAX supplement (Gibco), 0.5% MEM non-essential amino acids (Gibco) ...
-
bioRxiv - Neuroscience 2024Quote: ... 1% N2 supplement (Gibco) 1% GlutaMAX supplement (Gibco) ...
-
bioRxiv - Neuroscience 2024Quote: ... 1% GlutaMAX supplement (Gibco), 1% MEM non-essential amino acids (Gibco) ...
-
bioRxiv - Molecular Biology 2024Quote: ... with glutamine (1%, Gibco), ascorbic acid (50 ng/mL ...
-
bioRxiv - Physiology 2024Quote: ... 1:200 (Invitrogen A11039), Goat anti-Mouse IgG 488 (Invitrogen A11029) ...
-
bioRxiv - Neuroscience 2024Quote: ... 1% N2 (#17502048, Gibco), 1% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2024Quote: ... 1 % penicillin/streptomycin (Gibco) and 50 µM β-mercaptoethanol (Sigma ...
-
bioRxiv - Immunology 2024Quote: ... 1 % penicillin/streptomycin (Gibco) and 50 µM β-mercaptoethanol (Sigma) ...
-
bioRxiv - Biophysics 2024Quote: ... and 1 % penicillinstreptomycin (Gibco) at 37 °C with 5 % CO2 ...
-
bioRxiv - Genetics 2020Quote: ... Cells were seeded in 6-well plates and transfected with 4 μg/well vectors expressing CasRx-GFP and gRNAs-mCherry (CasRx:gRNA-1:gRNA-2 = 2:1:1, see Supplementary sequences) using Lipofectamine 3000 reagent (Thermo Fisher Scientific). Control group was only transfected with 2 μg/well vectors containing CasRx-GFP ...
-
bioRxiv - Immunology 2021Quote: ... was added to a concentration of 1X and 12.5-30 μg of total protein from each sample was resolved on NuPAGETM 4-12% BisTris (Phospho-PMK-1 and Total-PMK-1) or NuPAGETM 3-8% TrisAcetate (TIR-1::3xFLAG) gels (ThermoFisher Scientific), transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
-
bioRxiv - Immunology 2021Quote: ... We also used anti-prospero (1:100, DSHB), anti-armadillo (1:100, DSHB) as primary antibodies and Hoechst 33258 (1:500; Molecular Probes) for DNA staining ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were transferred to secondary antibody cocktail (Alexa Fluor 568 donkey anti mouse, 1:500; Alexa Fluor 488 donkey anti rabbit, 1:500; NeuroTrace Nissl 640/660, 1:500; all from Life Technologies) for 2-hour incubation at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Monolayers were incubated with rabbit anti-mouse zonula occludens-1 (ZO-1) antibody (1:50; cat# 61-7300, Invitrogen, Camarillo, CA) or rabbit anti-mouse occludin antibody (1:200 ...
-
bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed in 0.1% Triton X wash solution and then incubated in secondary antibody solution (1% goat serum, 1% Triton X, and 1:500 goat anti-rabbit conjugated with Alexa Fluor 594 (Thermo Fisher Scientific Cat# A-11037 ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 h with secondary antibodies (anti-Mouse IgG 488, 1:1000 Invitrogen, #A11029; anti-rabbit IgG 488, 1:1000, Invitrogen, #A11034), followed by DAPI staining (5 μg/ml).
-
bioRxiv - Neuroscience 2019Quote: ... Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636; Map-2 1:250, Sigma Aldrich M4403; Map-2 1:250, Invitrogen PA517646 ...
-
bioRxiv - Systems Biology 2021Quote: ... prior to immunostaining according to standard protocols [2,31] and using a primary antibody directed against zonula occludens-1 (ZO-1; 1:100, ThermoFisher Scientific, UK) or Alexafluor 488-conjugated phalloidin (1:140 ...
-
bioRxiv - Systems Biology 2021Quote: ... which contained advanced DMEM/F12 supplemented with penicillin/streptomycin (1%, 10 mM HEPES, 1× Glutamax, 1× B27 (all from Life Technologies) and 1 mM N-acetylcysteine (Sigma ...
-
bioRxiv - Microbiology 2020Quote: ... The membranes were washed and incubated with an HRP-conjugated secondary antibody (anti-mouse, 1:5,000, 31430; anti-rabbit, 1:5,000, 31460; anti-goat, 1:5,000, R-21459, Invitrogen, Grand Island, NY, USA). The membranes were then washed again ...
-
bioRxiv - Cell Biology 2020Quote: ... in PBS + 1% BSA +1% goat serum + 0.05% Tween Specific binding was detected with Goat anti-Rabbit IgG-AF488 (Invitrogen, 1:1000) for 1h at 37 ◦C while nuclei were stained with DAPI (1:1000) ...
-
bioRxiv - Bioengineering 2022Quote: ... The intestine organoids were isolated from Matrigel and resuspended in a chemical fragmentation buffer consisting of a 1:1 (vol:vol−1) solution of DPBS and TrypLE Express Enzyme (12605010; Thermo Fisher Scientific) supplemented with 10 μM Y-27632 ...
-
bioRxiv - Microbiology 2022Quote: ... a-tubulin (1:500, 2144, Cell Signalling), acetyl-a-tubulin (1:500, 1215, Cell Signalling) GAPDH (1:10,000, G9545, Invitrogen, Fisher Scientific) and GAPDH (1:10,000 ...
-
bioRxiv - Biophysics 2022Quote: ... maintained at 37 °C and 5.5% CO2 in DMEM and F-12 (1:1) media containing 10% FBS and 1% Penicillin/Streptomycin (Thermo Fisher Scientific). At first ...
-
bioRxiv - Immunology 2022Quote: ... malayi parasites were washed several times in worm culture media warmed to 37°C (RPMI with 1% HEPES, 1% L-glutamine, 0.2% Penicillin/Streptomycin, and 1% w/v glucose (Thermo Fisher Scientific)) ...
-
bioRxiv - Immunology 2020Quote: ... purified B cells were incubated at 6 × 106 cells ml-1 in 1 mL of PBS with 1 uL of 5 mM Cell Trace Violet (CTV) dye (Thermo Scientific). The CTV-stained B cells were washed and activated as detailed above and subjected to flow cytometry analyses 4 days after activation.
-
bioRxiv - Neuroscience 2020Quote: ... the spheroids were embedded to 20μl of matrigel and cultured in differentiation medium 1 (Diff1, 1:1 mixture of DMEM F12 and Neurobasal (Gibco #21103-049), 1x Glutamax ...
-
bioRxiv - Neuroscience 2020Quote: ... or Alexa 555 (1:500) and donkey anti-rabbit IgG conjugated to Alexa 488 (1:500) or Alexa 555 (1:500) (Molecular Probes). The samples were mounted in Vectorshield (Vector Laboratory) ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by an anti-mouse secondary antibody (V5: 1 hr, RT, 1/500; Invitrogen, Cat #A21200; FLAG: 2 hr, RT, 1:200; Invitrogen, #A11004) according to the manufacturer instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Orexin A/Hypocretin-1 (1:1000, R and D Systems, MAB763) Phospho-JUND (Ser255) (1:100, Thermo Fisher Scientific, PA5-104821), Phospho-nNOS (Ser1417 ...
-
bioRxiv - Biochemistry 2022Quote: ... The reactions were stopped with addition of 1 μl 1% SDS and 1 μl 10 mg/ml Proteinase K (Invitrogen™), and incubating at 37°C for further 5 m ...
-
bioRxiv - Cancer Biology 2020Quote: ... The UM92.1 cell line (a kind gift from Aart Jochemsen) were grown in a 1:1 mixture of RPMI 1640 (Gibco, Invitrogen) and DMEM/F12 (1:1 mixture ...
-
bioRxiv - Neuroscience 2021Quote: ... and then the in secondary antibody solution (1% goat serum, 1% Triton X, and 1:500 goat anti-rabbit conjugated with Alexa Fluor 594, Thermo Fisher Scientific Cat# A-11037 ...
-
bioRxiv - Physiology 2021Quote: ... followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121, Thermo Fisher, 1:50) and/or rabbit polyclonal TOM20 antibody (Santa Cruz ...
-
bioRxiv - Immunology 2020Quote: ... 100mM NaCl, 1 mM EDTA. 1% triton X-100 supplemented with Halt 1:100 protease Inhibitor cocktail Cat. No 78430, Thermo Scientific). After 30min on ice ...
-
bioRxiv - Microbiology 2020Quote: ... The cell line was then adapted to suspension culture and maintained in Expi293™ expression medium supplemented with 1% (v/v) penicillin-streptomycin and 1 μg ml-1 puromycin (Gibco).
-
bioRxiv - Microbiology 2020Quote: ... RNA samples (1 µg) were treated with DNase I (1 U/µl per 1 µg of RNA sample) (Thermo-Fisher Scientific), then reverse transcription carried out using the SuperScript™ IV first-strand synthesis system (Thermo-Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... 2.5 x105 CFSE-labeled CD8+ T cells were cultured with J774M cells at varying ratios (1:1 – 1:8 J774M:T cells) and anti-CD3/CD28 beads (ThermoFisher, cat. 11453D) at a bead-to-T cell ratio of 1:1 per the manufacturer’s instructions for T cell activation ...
-
bioRxiv - Molecular Biology 2020Quote: RL-118 drug was diluted to a concentration of 20 μM (DMSO<1%) in tissue culture medium (DMEM with 10% FBS, 1% L-Glutamine, 1% penicillin-streptomycin, (Life Technologies)) ...
-
bioRxiv - Neuroscience 2020Quote: ... a 1 µl Hamilton syringe (25s-gauge needle) filled with 150nL of 1% CT-B (1 mg/mL; Invitrogen, OR, USA) was used to pressure inject the retrograde tracer (150nL ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were subsequently washed with 0.05% PBST and stained with secondary antibody (Primary and Secondary antibody dilution provided in Table 1 Supplementary material) for 1 hr at RT and counterstained with DAPI (1:10000) (Life Technologies) for 1 minute and observed under fluorescent microscope Nikon Eclipse TE2000U.
-
bioRxiv - Cell Biology 2022Quote: ... Retinas were then washed five times with 1:1 blocking buffer in PBS for 20 min and incubated with Alexa Fluor streptavidin-conjugated antibodies (1:100; Life Technologies) for 2 hours at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... Phage DNA integrity was checked using agarose gel electrophoresis (1 % agarose gel in 1× TAE buffer; 70 V, 90 min) against a GeneRuler 1 kb ladder (ThermoFisher Scientific).
-
bioRxiv - Molecular Biology 2022Quote: ... generally according to the suppliers’ recommendations (1× EPAP buffer, 1 mM ATP, 1 mM DTT, and 0.8 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 1 h) ...
-
bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Life Technologies a11006 ...