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Citations for Thermo Fisher :
7151 - 7200 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... bath sonicated for 5 minutes and centrifuged at 13000g for 10 minutes at room temperature prior to quantification of total protein by a Pierce 660 Protein Assay (Thermo Scientific #22660). Condensates were obtained by centrifuging at 10,000xg for 10 minutes and resuspended in 5%SDS in 50 mM TEAB prior to total protein quantification ...
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bioRxiv - Molecular Biology 2024Quote: The protein concentrations of both fractions were determined in duplicate on the same day as protein isolations to minimize freeze-thaw effects using a commercially available assay (bicinchoninic acid [BCA] Protein Assay Kit; Thermo Fisher Scientific). Thereafter ...
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bioRxiv - Cell Biology 2024Quote: ... and then normalized to both the internal standard (d5-2HG) peak area and protein content of duplicate samples as determined by BCA protein assay (Thermo Fisher Scientific). Ions used for quantification of metabolite levels are as follows ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentrations of the lysed sEV preparations were determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA), according to the provided guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... We measured the total protein content in the prepared seeds by BCA quantification (Micro BCA™ Protein Assay Kit, Thermo Fisher Scientific). 2% (w/w ...
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bioRxiv - Biochemistry 2024Quote: The cell surface expression of wild-type and mutant hGPR30-HA was analysed via cell surface protein isolation using a Cell Surface Protein Isolation Kit (#89881, Thermo Scientific™) followed by western blotting ...
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bioRxiv - Bioengineering 2024Quote: ... Protein lysates were then electrophoresed on a NuPAGE Bis-Tris Protein Gel (4-12%) using NuPAGE SDS Running Buffer (Thermo Fisher Scientific) for 2 hr at 130 V before transfer to a nitrocellulose membrane in Towbin Buffer (20 mM Tris-HCl pH 8.3 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and then normalized to the concentration of total proteins assessed by using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... Cell debris were pelleted by centrifugation for 20 min at 4 °C and 20,000 x g and the total protein concentration of the cleared lysate was determined using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Scientific, #A53225) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: RNA-protein interactions were detected using Pierce RNA 3’ End Desthiobiotinylation Kit and Pierce Magnetic RNA-Protein Pull-Down Kit (Thermo Scientific, #20163), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The membrane proteins of liver non-parenchymal cells (NPCs) were purified using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific)
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentration of Triton X-100 soluble and insoluble fraction was determined using BCA Protein Assay Kit (Thermo Fisher Scientific Inc).
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations of both the cytosolic and membrane fractions were measured using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and equal amounts of protein were loaded on to a NuPAGE 4-12% Bis-Tris gel (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... 200μg of protein was used for click chemistry and was performed using biotin-alkyne and protein reaction buffer kits (Thermo Fisher: C10276). AHA-biotin-alkyne labeled proteins were pulled down with streptavidin beads and the indicated proteins were analyzed by immunoblotting.
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bioRxiv - Cancer Biology 2021Quote: ... and protein digested with Proteinase K (ThermoFisher Scientific, 25530049). Samples were purified with DNA Clean and Concentrator columns (Ozyme ...
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bioRxiv - Cancer Biology 2021Quote: ... Rabbit anti-Myelin Basic Protein (BMP) (ThermoFisher; 1:300), Mouse anti-NeuN (Abcam ...
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bioRxiv - Systems Biology 2020Quote: ... Protein concentrations were analyzed with BCA assay (Thermo Scientific Pierce BCA Protein Assay Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μl Dynabeads Protein G (Thermo Fisher Scientific, 10004D), and 400 μl dilution buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... protein concentration was measured by BCA assay (Thermo Scientific), and protein concentrations were equalized prior to boiling in the recommended sample buffers supplemented with 2.5% beta-mercaptoethanol (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... protein A and G Beads (10002D, 10003D; Life Technologies) were first blocked with 5mg/ml BSA (A7906 ...
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bioRxiv - Cancer Biology 2021Quote: ... BCA protein assay kit (Thermo Fisher Scientific, MA, USA) was used measuring protein concentration per manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by binding to Protein-G Dynabeads (Invitrogen, 10003D) for 20 minutes hours at 4°C to enrich for endothelial cell nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were blotted on nitrocellulose membrane (Fisher scientific). The membrane was blocked in 5% milk/TBS-T for 1 h followed by overnight incubation with primary antibody at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 µL Protein G DynaBeads slurry (Invitrogen 10004D) overnight at 4 °C in an end-over-end mixer ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 µL Protein G DynaBeads slurry (Invitrogen 10004D) overnight at 4 °C in an end-over-end mixer ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was transferred to a nitrocellulose membrane (ThermoFisher, LC2000) and then blocked with Odyssey Blocking Buffer (Li-Cor Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Total protein was extracted using either TRIzol reagent (Invitrogen) as recommended or RIPA buffer (50mM Tris-HCl,pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... was pre-bound to protein G Dynabeads (Invitrogen 10004D) in buffer A supplemented with 14% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... Purified proteins were quantified by Bradford assay (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was visualized with ECL SuperSignal (Thermo Scientific) according to manufacturer’s instructions on Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: Proteins were extracted by TRIzol Reagent (#15596018, Invitrogen, USA) and resolved in 1% SDS ...
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bioRxiv - Developmental Biology 2021Quote: ... The proteins were quantified by Qubit 2.0 (Life Technologies) and stored in aliquots at -20°C.
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bioRxiv - Immunology 2021Quote: ... proteins were transferred with an iBlot instrument (Life Technologies) to a nitrocellulose membrane ...
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bioRxiv - Immunology 2021Quote: ... IgG was purified using protein G affinity chromatography (Invitrogen).
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bioRxiv - Immunology 2021Quote: ... Proteins were transferred to polyvinylidene difluoride membranes (iBlot; Invitrogen). Membranes were blocked with TBST containing 3% BSA and incubated with antibodies against IRF1 (H-8 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with Dynabeads Protein G (Life Technologies) for 4 hours ...
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bioRxiv - Genetics 2021Quote: ... A mixture of sgRNA and Cas9 protein (Thermo Fisher) was incubated at room temperature for 15 min and microinjected into preblastoderm embryos within 5 h of oviposition ...
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bioRxiv - Genetics 2021Quote: ... Separated proteins were transferred to PVDF membranes (Thermo Scientific) by electroblotting ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were electro-transferred to nitrocellulose membranes (Invitrogen) for 1 h at 100 V ...
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bioRxiv - Microbiology 2021Quote: ... protein A/G magnetic beads (Cat. # 88802, Thermo Scientific) and anti-FLAG M2 magnetic beads (Cat ...
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bioRxiv - Cell Biology 2020Quote: The Subcellular Protein Fractionation Kit for cultured cells (ThermoFisher) was used to isolate the cytoplasm ...
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bioRxiv - Molecular Biology 2021Quote: ... immobilized on 5 µl of Protein G Dynabeads(Invitrogen) for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Alexa Fluor 647-protein labeling kit was from Invitrogen.
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bioRxiv - Developmental Biology 2021Quote: ... The guide and TrueCut Cas9 v2 protein (ThermoFisher A36497) were electroporated with a NEPA21 (NEPA GENE Co ...
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bioRxiv - Immunology 2020Quote: ... Protein samples were separated by SDS–PAGE (Invitrogen #NW04125BOX), immunoblotted and visualized using ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Fifty microliters of Dynabeads Protein-A (Life Technologies, 10002A) were added to the mixture and incubated at 4 °C for 2 h ...
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bioRxiv - Developmental Biology 2020Quote: ... alongside 5 μl PageRuler Prestained Protein Ladder (Thermo Scientific), and electrophoresed at 100-200 V for one to two hours in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (BIORAD) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were quantified with BCA reagent (Thermo Fisher) and analysed by SDS-PAGE ...
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bioRxiv - Immunology 2022Quote: ... Protein concentrations were determined using BCA assay (ThermoFisher Scientific). 20 µg of total denatured bacterial protein was loaded into each well in SDS gel ...
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic beads (DynaBeads, Protein A, Thermo Fisher Scientific, #10002D) were pre-blocked in a 0.5% Bovine Serum Albumin (BSA ...