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Citations for Thermo Fisher :
7001 - 7050 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... followed by C-terminal region of human FGF-21 (amino acids 166-209) into a vector pCEP4 (Thermo Fisher Scientific). The construct was then transiently transfected to Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The brain sections were then incubated with goat anti-mouse Alexa Fluor 488 and goat anti-human Alexa Fluor 647 (1:500, Invitrogen). Following secondary staining ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... The donor template for homologous recombination was generated by PCR amplification of a human DNA sample carrying 51 CAG repeat into the pCR-Blunt II TOPO plasmid (Invitrogen). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... in 6-well tissue culture plates at 37°C with 5% CO2 and 5% O2 They were grown in human embryonic stem cell (hESC-10) medium made up of a base of DMEM/F12 (50:50 Gibco) with 20% knock-out serum replacement (KOSR ...
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bioRxiv - Bioengineering 2023Quote: Undifferentiated H1 (WA01; XY) human embryonic stem cells (hESCs) were maintained on Geltrex coated plates (1:100 in DMEM/F12; Gibco) in StemFlex Medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: NIH 3T3 immortalised mouse fibroblast and U2OS human osteosarcoma cell lines were cultured in Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher), supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice with PBS and 100 μL of Goat anti-human IgG (H+L) Alexa647 secondary antibody (2 μg/mL, Invitrogen) and Aqua viability dye was added for 20 minutes at RT ...
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bioRxiv - Immunology 2022Quote: ... and activated in complete RPMI supplemented with 30 U/mL IL-2 with anti-human CD3/CD28 dynabeads (11131D, ThermoFisher) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney cells (HEK293T, ATCC, Catalog# CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: iPSCs colonies were harvested with Accutase® and dissociated into single cells using the complete human embryonic stem cell (hESC) medium consisting of DMEM/F12 (11320033, ThermoFisher), 5x knockout serum replacement (10828010 ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... hTDP-43 mRNA was measured using the PrimeTime Gene Expression Master Mix (IDT) with the following TaqMan probes: human TDP-43 (Hs00606522_m1; ThermoFisher Scientific) and mouse Actb (Mm02619580_g1 ...
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bioRxiv - Immunology 2024Quote: All the siRNA-mediated knockdown experiments in primary human macrophages were performed by electroporation using a Neon Transfection System (MPK5000; Invitrogen). Each reaction used 1.2 – 1.5 x 106 hMDM ...
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bioRxiv - Systems Biology 2024Quote: All RAW files acquired from TMT-MS of all cases were searched against a human reference protein database using the Proteome Discoverer suite (version 2.4, ThermoFisher Scientific). MS2 spectra were searched against the UniProtKB human proteome database containing Swiss-Prot human reference protein sequences (20,338 target proteins downloaded in 2019) ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-10A cells were transfected with the human ON-TARGET plus siRNA library targeting PHGDH (Dharmacon) or non-target control (Dharmacon) using the Neon Transfection System (Invitrogen). Then 96 hours post transcription ...
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bioRxiv - Neuroscience 2024Quote: The quantification of the total amount of soluble tau in S1 fractions was performed by using an ELISA kit (human Tau, Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... human ileocecal cell line from 25 to 35 passages were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 4mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... buffer (1× PBS containing 2% FBS) followed by incubation with Alexa Flour 633 goat anti-human IgG (1: 2000 dilution, Invitrogen). Finally ...
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bioRxiv - Microbiology 2023Quote: ... cells and human embryonic kidney 293T cells (ATCC) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... One hundred microliters of cell suspension from each well was stained with anti-human CD45 (anti-human CD3 for NK cell experiments) according to the manufacturer’s instructions and washed twice with PBS (Gibco, USA) (300×g ...
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bioRxiv - Molecular Biology 2023Quote: ... One hundred microliters of cell suspension from each well was stained with anti-human CD45 according to the manufacturer’s instructions and washed twice with PBS (Gibco, USA) (300×g ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from human hippocampal/frontal cortex samples and cultured cells was extracted with mirVana’s isolation kit (Ambion, TX, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of Fetal Bovine Serum (Fisher) and 125 μl of a 4 mg/ml human insulin stock solution (Gibco) were added ...
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bioRxiv - Bioengineering 2023Quote: Blood plasma extracted from each group (n=5) was analyzed using the Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen). Briefly ...
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bioRxiv - Biophysics 2023Quote: BHK-21 and CFBE41o- human CF bronchial epithelial cell lines were grown in DMEM/F-12 (5% fetal bovine serum; Invitrogen) and MEM (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: Protein from rat and human fetal lung explants was isolated by incubation with cell extraction buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease inhibitors (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 300,000 cells were transfected with 50nm ON-TARGETplus Human SMARCA4 siRNA (Dharmacon L-010431-00-0005) or the corresponding siControl (D0012061305) by lipofectamine RNAiMAX (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: DLD 1 human colorectal cancer and Colon26 mouse colon cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM)(Gibco, USA) containing 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2023Quote: ... MSC-EV preparation vesicular structure and canonical surface marker expression were determined using EV bead-based flow cytometry with human CD81 detection beads (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: We used the cytokine/chemokine/growth factor 45-Plex Human ProcartaPlex™ Panel 1 (Cat# EPX450-12171-901, ThermoFisher, USA) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... Lipofectamine™ 3000 transfection reagent (InvitrogenTM, Cat# L3000001) and human PAR-1 si-RNA (CGGUCUGUUAUGUGUCUAUdTdT) were purchased from Invitrogen (USA) and Eurogentec (Belgium ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 × 106 human cells in 35 mm dish per sample were washed with 1 × PBS and lysed with 600 μl DNAzol (Invitrogen), followed by precipitation with 300 μl 200 proof ethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... at different timepoints as the percentage of hCD20pos cells (anti-human CD20 FITC, clone 2H7) compared to murine CD45pos cells (anti-mouse CD45 PE, clone 30F11, ThermoFisher). When indicated ...
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bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: African green monkey kidney cells (Vero) and Human Embryonic Kidney 293T (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, USA) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Human A549 or A549ACE2 cells were cultured in a Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco catalog no. 11875) supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... in this study were generated from deidentified commercially available human tracheal epithelial cell lines via the manufacturing protocol of Sendai virus-mediated reprogramming (CytoTune2.0) (Thermo Fisher) as described before24 ...
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bioRxiv - Cell Biology 2024Quote: ... used in this study were previously derived from in-house grown dermal fibroblasts from three healthy human donors reprogrammed with Cytotune Sendai viruses (ThermoFisher) (Ethics Committee ...
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bioRxiv - Cell Biology 2024Quote: Human induced pluripotent stem cells (iPSCs) were obtained from Stanford Cardiovascular Institute and cultured in E8 medium (Gibco, Life Technologies) on 6-well plates coated with Matrigel (Corning) ...
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bioRxiv - Cell Biology 2024Quote: Human induced pluripotent stem cells (iPSCs) were obtained from Stanford Cardiovascular Institute and cultured in E8 medium (Gibco, Life Technologies) on 6-well plates coated with Matrigel (Corning) ...
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bioRxiv - Pathology 2024Quote: ... genomic DNA isolated from blood lymphocytes was subjected to exome capture using Agilent SureSelect human exome capture arrays (Life Technologies), followed by next generation sequencing on the Illumina® sequencing platform ...
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bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with goat anti-human IgM-FITC (1:100 dilution, Cat. no. A18842, Invitrogen), and IgG-FITC (1:200 dilution ...
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bioRxiv - Genetics 2024Quote: ... Purified human primary pancreatic ductal cells (HPPD) were immediately cultured in a low-attachment plate in RMPI DMEM/F12 medium (Gibco), supplemented with 10% FBS and penicillin/streptomycin ...
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bioRxiv - Genetics 2024Quote: ... Purified human primary pancreatic ductal cells (HPPD) were immediately cultured in a low-attachment plate in RMPI DMEM/F12 medium (Gibco), supplemented with 10% FBS and penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... The plate was washed three times using 200 µL of 1 x PBS containing 0.5 % Tween-20 and incubated with 100 µL of secondary antibody solution containing goat anti-human IgG conjugated with HRP (Cat. #A18805 from Invitrogen) at a dilution of 1:10000 in 5% BSA solution for 1hour ...
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bioRxiv - Microbiology 2024Quote: ... with a plasmid encoding for a human furin ectodomain (residues 1–794) into 40 mLs of FreeStyle 293-F cells (ThermoFisher). Cells were transfected at a density of 1 million cells/mL using 25 kDa linear polyethylenimine (PEI) ...
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bioRxiv - Microbiology 2024Quote: ... and FCR-CD36 strains were maintained in culture under standard conditions in O+ human erythrocytes in RPMI 1640 containing L-glutamine (Invitrogen) supplemented with 5% Albumax I ...
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bioRxiv - Biophysics 2024Quote: The immortalized human keratinocytes (HaCaT cells) were cultured in low calcium media made from no-calcium DMEM (21068028, ThermoFisher Scientific), 10% FBS and 1% penicillin-streptomycin at 37°C supplied with 5% CO2 to maintain the dividing state.
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bioRxiv - Immunology 2024Quote: ... Freshly purified CD8+ T cells (day 0) were activated by adding Dynabeads™ Human T-Activator CD3/CD28 (Gibco™) at a cell/bead ratio of 1:0.5 for 48-64h and expanded in complete R10 medium [RPMI-1640 medium containing 20 mM HEPES and 2.05 mM L-glutamine (#7388 ...