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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Solid phase ELISA was performed by coating recombinant NEDP1 (20μg/well) on Maxisorb 96 well plates (Nunc) at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: Clones of pFastBac1 constructs were used to generate recombinant bacmids in DH10Bac following the manufacturers protocol (ThermoFisher). Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus) ...
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bioRxiv - Genomics 2020Quote: ... cells were serum-starved (with 0.3% SCS) for 72 hours prior to induction of gene expression with 0.1µg/mL recombinant EGF (Gibco) or PBS for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: Recombinant PfbA (rPfbA) or BSA was coated onto 0.5-µm-diameter fluorescent beads (FluoroSphere, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 ng of poly(dI-dC) were used for 100 ng of recombinant ERα (ThermoFisher, RP-310) and 1 µg for 10 µg of nuclear extracts ...
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bioRxiv - Biophysics 2021Quote: ... and 10 μg/mL Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 555 Conjugate (CTSB, Fisher Scientific) to label GM1 as the acceptor ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated with 100 U/mL mouse recombinant IFN-γ (Invitrogen, Carlsbad, CA, USA) for 24 hours and subjected to flow cytometry analysis of PD-L1 expression as described above.
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bioRxiv - Cancer Biology 2022Quote: ... Matrigel droplets were overlaid with recombinant organoid medium: Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were prepared in Spodoptera frugiperda (Sf9) cells using the Bac-to-Bac system (Life Technologies). Recombinant protein complexes were expressed in Spodoptera frugiperda by co-infection of single baculoviruses ...
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bioRxiv - Neuroscience 2021Quote: ... was added to each sample (4μl 5X First-Strand Buffer, 1μl 0.1 M DTT, 1μl RNaseOUT Recombinant RNase Inhibitor (Invitrogen; 10777019), 1μl of SuperScript III RT (200 units/μl) ...
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bioRxiv - Developmental Biology 2021Quote: ... fibroblast outgrowths were dissociated and split 1:4 using a recombinant trypsin-like enzyme (TrypLE Select, Invitrogen). Additional fibroblast preparations were commercially available ...
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bioRxiv - Immunology 2021Quote: ... The recombinant Fab was purified from culture supernatant using an anti-CH1 CaptureSelect column (Thermo Fisher Scientific). For the RBD/COV2-2196 complex ...
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bioRxiv - Molecular Biology 2022Quote: ... coli expression plasmids for purification of recombinant Cdc14 enzymes were generated using the Gateway cloning system (Invitrogen). S ...
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bioRxiv - Biochemistry 2024Quote: ... The Dicer/TRBP enzyme was the mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were subsequently incubated by primary antibody 1:500 (GFP recombinant rabbit monoclonal antibody; G10362, Life Technologies) for 48 hours at 4°C followed by three PBS washes ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant adenoviruses expressing the GRK2 shRNA were produced using BLOCK-iT™ Adenoviral RNAi Expression System (Invitrogen) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2022Quote: ... and 50 seconds elongation at 72°C with 1U of Pfu recombinant DNA polymerase (Thermo Fisher Scientific). The amplicon ORF45HA was cut with NheI/SmaI and inserted in pEGFP-C1 (Clontech) ...
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bioRxiv - Biophysics 2023Quote: ... recombinant baculoviruses were generated in Sf9 cells using the Bac-to-Bac baculovirus expression system (Invitrogen, USA). HEK293F cells were cultured under 5% CO2 at 37 °C and were used for transfection at a density of 2.5 × 106 cells/ml ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were performed by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) and carried out in a total volume of 25 µL containing the following ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cardiomyocytes were labeled with of DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17, Invitrogen, MA1-142-A488) at 1:500 dilution in 1% BSA ...
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bioRxiv - Microbiology 2023Quote: ... 15μL of annealed guide RNA oligos were mixed with 15μL of 1μM recombinant Cas9 (IDT) in 220μL of Opti-MEM media (Gibco) and incubated for 5min at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Enzymes used for recombinant DNA cloning and Golden Gate Assembly were purchased from Thermo Scientific (Waltham, MA) and New England Biolabs (Ipswich ...
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bioRxiv - Immunology 2024Quote: ... either the recombinant CT584 or purified Cm EB’S were solubilized in SDS sample buffer (Novex, Life Technologies) in the presence of reducing agent (10mM DTT) ...
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bioRxiv - Plant Biology 2019Quote: ... Protein concentrations were determined using Pierce 660 nm protein assay (Thermo Scientific). Extracts with 500 μg of protein were alkylated with 14 mM chloroacetamide (CAA ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentration was measured by EZQ protein quantification kit (Thermo Fisher Scientific), and 30 µg of protein from each sample was reduced with 10 mM dithiothreitol overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured by BCA Protein assay kit (Thermo Fisher Scientific) at 562 nm using BSA to produce a standard curve ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) and purity was checked by sodium-dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined by a BCA Protein Assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: Protein concentration was determined using a BCA protein assay kit (ThermoFisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The amount of protein was determined using QuBit Protein Assay Kit (ThermoFisher) following the manufacturer’s instruction and 80 ug of protein was loaded per well ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A beads (Dynabeads Protein A, ThermoFisher Scientific, Waltham, MA, Cat #10002D) were added and samples were incubated at 4°C for 4 hrs ...
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bioRxiv - Genetics 2020Quote: ... Protein concentration was determined using BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Protein immunoprecipitation was performed using Dynabeads™ Protein G for Immunoprecipitation (Invitrogen) as per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D). 30 µL of beads were used per IP ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by the BCA protein assay kit (Thermo Fisher). The protein solution was aliquoted into a small volume ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Thermo Fisher Scientific). The lysates were fractionated with SDS– PAGE ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Thermo Fisher Scientific). Then ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined with BCA protein assay (Thermo Fisher Scientific, 23225), and equal protein amounts were used in denaturing gel electrophoresis.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (ThermoFisher). SDS-PAGE was used to measure beta glucocerebrosidase (GCase ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was measured using Coomassie Plus Protein Assay Reagent (Thermo scientific) and equal amounts of protein were loaded onto 6% SDS gels for anti-V5 and anti-TIM blots or 8% SDS gels for anti-CLK blots respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate protein concentration was measured with BCA Protein Assay (Thermo Fisher 23252). Lysate was diluted to 4 mg/ml in Sup6-150 ...
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bioRxiv - Molecular Biology 2021Quote: Protein content was determined using the Pierce BCA protein assay (Thermo Fisher). 4-10 μg of total protein in Laemmli sample buffer was resolved by SDS-PAGE on 9% polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were measured by QuBit Protein Assay Kit (Thermo Fisher, Q33211) and sarkosyl buffer (50mM HEPES pH 7.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration was measured with Qubit Protein Assay Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentrations were determined using BCA Protein assay Kit (Thermo Scientific Pierce). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2020Quote: ... separating proteins on NuPage™ 4-12% Bis-Tris Protein Gels (Invitrogen) followed by transfer onto nitrocellulose or PVDF (for IR-Dye detection ...
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bioRxiv - Cell Biology 2022Quote: Solubilized sperm proteins were quantified using EZQ Protein Quantitation Kit (Thermo Fisher) and 2.25ug alkylated (Pierce ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: ... Protein concentration was determined using the BCA protein assay kit (ThermoFisher, #23225). 20 to 30 μg of protein extract was separated on 4-12% Bis-Tris plus gels ...
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bioRxiv - Cell Biology 2022Quote: ... and their protein levels measured with Pierce BCA Protein Assay kit (Thermofisher). EGFP-INPP5E or its mutants were then immunoprecipitated from 1 mg of cell lysate by overnight rotation at 4°C with GFP-Trap_MA beads (Chromotek) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was quantified using Pierce BCA protein assay kit (Thermo Fisher). Equal amount of protein were mixed with 5X SDS sample buffer [5%(vol/vol ...