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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... alvi M0351 and the MF bees were dissected in PBS containing 50 μM intracellular ROS-sensitive fluorescent dye dihydroethidium (Invitrogen, #C10422). The tubes were placed in the dark for 10 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The secondary fluorescent antibody Alexa Fluor 488 (goat anti-mouse) and ProLong™ Gold Antifade Mountant with DAPI were from ThermoFisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated for 1 hour at room temperature in secondary antibodies conjugated with Alexa Fluor fluorescent probes (Thermo Fisher Scientific) diluted 1:400 in 1% PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and a green fluorescence (GFP) signal was visualized and recorded using an EVOS FL tabletop fluorescent microscope (ThermoFisher Scientific; Waltham, MA).
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bioRxiv - Neuroscience 2022Quote: ... and a fluorescent Alexa 594-conjugated goat anti-chicken IgG secondary antibody (1:500; Molecular Probes cat#A-11042, RRID: AB_2534099). Finally ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... sections were incubated at room temperature with species- and isotype-appropriate fluorescent dye-conjugated secondary antibodies and coverslipped using Prolong Gold anti-fade mounting medium (ThermoFisher Scientific). The following primaries were used for immunofluorescence in the present study ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS and then incubated in PBS containing 5 % normal goat serum with fluorescent conjugated secondary antibodies (Alexa Fluor 555-conjugated goat anti-mouse IgG, 1:500, Thermo Fisher, A21422 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with DAPI and Neurotrace 500 fluorescent Nissl stain and were mounted in ProLong Gold Antifade Mountant (ThermoFisher Scientific). Sections were imaged on a Nikon TiE fluorescence microscope equipped with NIS-Elements Advanced Research imaging software (v4.20) ...
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bioRxiv - Biophysics 2022Quote: ... for each experiment the two-color channels must be aligned using a slide with multi-spectral fluorescent beads (0.1 micron TetraSpeck beads, Thermo Fisher #T7279). Precise alignment is then performed computationally by using reference images of the bead slide in the color channels ...
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bioRxiv - Cell Biology 2024Quote: ... Cell proliferation was evaluated by quantitating cellular DNA content with fluorescent CyQUANT® Cell Proliferation Assay (Thermo Fisher Scientific, Waltham, MA). Cell proliferation was also evaluated by bromodeoxyuridine (BrdU ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescent lipids used in the study were Marina Blue™ 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (Marina Blue™ DHPE) (Invitrogen) and Forte Blue DHPE (AAT Bioquest).
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm, 660/680, F8807, Thermo Fisher Scientific), 6 µL 10% v/v tetramethylethylenediamine (TEMED ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently visualized with Alexa Fluor fluorescent secondary antibodies (goat anti-rabbit IgG Alexa 519; Thermo Fisher Scientific; 1:1,000) matched to host primary by incubating 2 hours at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... red fluorescent images of the uppermost layer of embedded beads were captured along with phase-contrast images of the selected regions every 30 minutes using an automated fluorescent microscope (20× objective, EVOS m7000, ThermoFisher Scientific) for a total time of 48 hours ...
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bioRxiv - Cell Biology 2024Quote: The surface roughness of substrates was tested by overlaying a solution of fluorescent beads (1 µm in diameter, F8816, ThermoFisher Scientific). 3.2 µL of the bead stock solution was mixed in 500 µL Matrigel and overlaid onto glass or gel-coated coverslips for 20 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... The cephalic bristle field region was dabbed with the fluorescent dye Alexa Fluor 488 (10,000 MW, Anionic, emission at 519 nm and excitation at 495 nm, D22910, Thermo Fisher Scientific) (Sant and Sane ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time polymerase chain reaction was carried out using a fluorescent temperature cycler (TaqMan®; Applied Biosystems, Foster City, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... using 200 psi and µm tungsten particles coated with fluorescent 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (DiI, Thermo Fisher Scientific; D-282). The DiI was allowed to diffuse at r.t ...
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bioRxiv - Neuroscience 2024Quote: ... a streptavidin-Alexa Fluor 594 conjugate (1:500) and 0.4% NeuroTrace 435/455 Blue Fluorescent Nissl stain (Thermo Fisher Scientific; N21479) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with a primary antibody overnight at 4°C followed by incubation with fluorescent-conjugated secondary antibodies (Molecular Probes) for 1 hour at room temperature ...
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bioRxiv - Genetics 2024Quote: ... each forward primer was labeled at the 5’ end with fluorescent labels (HEX and 6FAM, Integrated DNA Technologies; NED, Applied Biosystems). PCR was performed in 25 µL reaction volumes containing nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: ... to form condensates (with 20% v/v PEG 8 kDa as needed) and overlaid with fluorescent dextrans of various sizes at 10 kDa (Invitrogen D1816), 40 kDa (Invitrogen D1842) ...
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bioRxiv - Cell Biology 2024Quote: ... A second fixation was performed with 4% PFA for 15 minutes and then fiducials markers were added by incubating with 0.1 µm TetraSpeck Fluorescent Microspheres (Thermo Fisher Scientific). For imaging ...
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bioRxiv - Microbiology 2024Quote: ... respectively as described above followed by incubation with a goat anti-rabbit fluorescent secondary antibody (Invitrogen Goat anti-Rabbit Alexa Fluor Plus 568™, Cat# A-11036, 1:1000 or Invitrogen Goat anti-Rabbit Alexa Fluor™ Plus 647 ...
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bioRxiv - Bioengineering 2023Quote: ... 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm, 660/680, F8807, Thermo Fisher Scientific), 6 µL 10% v/v tetramethylethylenediamine (TEMED ...
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bioRxiv - Cell Biology 2023Quote: ... multicilia were labeled with 100 nM SiR-tubulin for 1 h and small fluorescent latex beads with a diameter of 250 nm at 1:500 dilution (Invitrogen, F8809) were added to the culture medium ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were transfected with pEGFP-C1 vectors and cultured on polyacrylamide gels with embedded 200-nm fluorescent beads (Thermo Fisher Scientific) for 2 days ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then washed 2X for 10 minutes in PBS1X and incubated for 1h at room temperature with blocking solution containing the following fluorescent secondary antibodies: anti-mouse IgG Alexa Fluor 488 and anti-rabbit IgG Alexa Fluor 594 (Thermo Fisher) diluted 1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... and stained the same day of the experiment with the fluorescent dye 5(6)-TAMRA/SE™ (Thermo Fisher Scientific, C1171) at a final concentration of 1 μM (as in Wehrl et al ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed 4 times for 15 min in PBS-T and then incubated with a fluorescent secondary antibody (1:2000 in PBS-T; goat anti-mouse IgG1 conjugated to Alexa Fluor 488; Invitrogen; RRID:AB_2534069). The tissue was washed twice with PBS ...
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bioRxiv - Microbiology 2023Quote: ... 100 µl of the above cell suspension was mixed with 1×106 uncoated carboxylated yellow-green fluorescent polystyrene microspheres (Molecular Probes) and incubated at room temperature for 30 min with rotary shaking at 150 rpm ...
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bioRxiv - Genomics 2023Quote: ... The primary antibody was visualized with a fluorescent secondary antibody conjugated to Alexa Fluor 488 raised in goat (1:1000, Invitrogen A21206) (1 hr) ...
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bioRxiv - Biophysics 2023Quote: ... The sample was incubated in a sterile medium at 37°C with the fluorescent calcium-dependent Fluo-4 AM (Invitrogen, USA) dye in concentration of 4 μg/ml for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Neuronal somata were visualized with a stain against the Nissl substance by using the NeuroTrace 435/455 fluorescent dye (1:200 dilution, Invitrogen; #N21479). All secondary antibodies were raised in goats ...
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bioRxiv - Physiology 2023Quote: ... Samples were then diluted 4x with 1x filtered PBS and spiked with 10 µl of 1:1000 diluted fluorescent bead stock (Life Technologies 4.0 µm yellow-green 505/515 Fluospheres sulfate) ...
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bioRxiv - Neuroscience 2023Quote: ... and neuronal nuclei were visualized either using fluorescent Nissl stain (NeuroTrace 435/455 or NeuroTrace 530/615, N21482, Thermo Fisher Scientific) or DAPI (Fluoromount-G mounting medium ...
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bioRxiv - Immunology 2023Quote: A cell-based microplate assay was used to measure intracellular free zinc ions (Zn2+) using the cell-permeable fluorescent probe FluoZin-3 AM (Invitrogen, #F24195). BMDMs were plated into 96-well clear-bottom black polystyrene microplates (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were washed with TBST and incubated with the required fluorescent-dye conjugated secondary antibody (1:1000) for 1 h at RT (Alexafluor, Invitrogen, Thermofisher). Slides were mounted with DAPI Pro-LongDiamond antifade mountant overnight and analyzed the next day ...
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bioRxiv - Bioengineering 2023Quote: ... The specimens were subsequently washed three times with PBS and were incubated with a secondary fluorescent goat anti-mouse AlexaFluor647-conjugate (A21235, ThermoFisher Scientific) at a dilution of 1:1000 in the blocking solution of PBS containing 1% BSA together with 300 nM DAPI nuclei staining ...
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bioRxiv - Biochemistry 2022Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 G614 or its mutants or S2 together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... The viability of each well was assessed by double fluorescent staining with 0.5 μg/ml Hoechst and 100 nM YOYO1 (Invitrogen, Waltham, MA). Four images for each well were captured under 4X magnification using Thermo Scientific CellInsight NXT high-content screening platform ...
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bioRxiv - Immunology 2023Quote: ... in the same blocking solution overnight for 16 h and then with Alexa Fluor 594-conjugated donkey anti-rabbit IgG secondary fluorescent antibody (1:500 dilution, Invitrogen A21207) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The spherical aberration correction was conducted before every imaging session by using sub-resolution fluorescent beads (PS-Speck, Thermo Fisher Scientific). This was followed by illumination pattern alignment and optimization for the used laser lines (488nm ...
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bioRxiv - Physiology 2023Quote: ... Registration of the two color channels was accomplished using localized positions of several TetraSpeck Fluorescent Microspheres (ThermoFisher Scientific, Carlsbad, CA, USA) scattered throughout the field of view ...
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bioRxiv - Cell Biology 2023Quote: For flowcytometric quantification of GFP signal in induced 3D7-DiCre pFIO/pFIO+ strains 2.5 µl iRBCs were washed once with RT 0.9% NaCl solution (B. Braun GmbH) and stained with 5 µM SYTO 61 Red Fluorescent Nucleic Acid stain (Thermo Fisher Scientific) for 1 h at 37°C in 0.9% NaCl solution and washed once again afterwards at RT ...
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bioRxiv - Neuroscience 2023Quote: ... and whole media fractions were mixed with the fluorescent membrane dye Octadecyl Rhodamine B Chloride R18 (0.33 ng/ul, Life Technologies, O246) and incubated for 10 min at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Registration of the two color channels was accomplished using localized positions of several TetraSpeck Fluorescent Microspheres (ThermoFisher Scientific, Carlsbad, CA, USA) scattered throughout the field of view ...
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bioRxiv - Bioengineering 2023Quote: ... we used an mRNA encoding enhanced green fluorescent protein (eGFP) with ARCA cap modifications (Apexbio Technologies, Fisher Scientific #50-199-8310). For Cas9 RNP complex transfection ...
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bioRxiv - Neuroscience 2023Quote: ... Rats were anesthetized using vaporized isoflurane and bilaterally injected with 5 µg of Alexafluor-555 labeled fluorescent choleratoxin subunit b (fCTb) (Invitrogen C34776) into the left and right side of the ARC using the following coordinates from bregma ...
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bioRxiv - Neuroscience 2023Quote: ... of the probe were coated with red-fluorescent dye 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI, D-282, ∼10% in ethanol, Thermo Fisher Scientific, Waltham, MA, USA) prior to insertion ...