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Citations for Thermo Fisher :
651 - 700 of 7949 citations for Tau Methyl L Histidine Methyl D3 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... GFP/6xHis and also GFP-Tau (GFP-hTau40/6xHis; 2N4R) and (hTau40/-GFP/6xHis; 2N4R) were expressed in Sf9 insect cells from Invitrogen. Sf9 cells were infected with recombinant virus at a MOI of > 1 typically in six T150 cell culture flasks containing 75% confluent Sf9 cells ...
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bioRxiv - Pathology 2022Quote: ... and phosphorylated paired helical filament tau with phospho-Ser202+Thr205 using a monoclonal antibody (AT8, 1:1,000; MN1020, ThermoFisher [47]). Endogenous peroxidase was quenched by 20 min incubation in 0.1 M sodium periodate ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated in primary antibody diluted in blocking buffer using the following primary antibodies: anti-Phospho-Tau (Ser396) (1:500, 44-752G, Invitrogen), anti β-catenin (E-5 ...
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bioRxiv - Neuroscience 2023Quote: ... and blocked with 3% normal goat serum in Tris-buffered saline for immunostaining with the phospho-tau (Ser202, Thr205) AT8 antibody (1:200, MN1020, Invitrogen).
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with monoclonal anti-phosphorylated (p)tau (Ser202, CP13, 1:50; Ser202/Thr205, AT8, ThermoFisher MN1020, 1:50) or anti-Aβ (6E10 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with various tau species (at a final concentration of 2 μM unless stated otherwise) using 1.25 μL of Lipofectamine 2000 (Thermo Fisher) per well ...
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bioRxiv - Neuroscience 2023Quote: ... the immediate adjacent sections (4-µm thickness) were stained against phospho-tau AT8 (pS202/pT205, 1:1,000 dilution, Thermo Scientific) using the Autostainer Link 48 (Dako) ...
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bioRxiv - Cell Biology 2023Quote: ... the NPC line used is designated MGH2046-RC1 and is derived from an individual in her 50s with FTD and carrier of the tau-P301L autosomal dominant mutation (c.C1907T, rs63751273).31,188 NPCs were cultured in 6-well (Fisher Scientific Corning) or black 96-well clear bottom (Fisher Scientific Corning ...
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bioRxiv - Immunology 2022Quote: ... 4.5 g/L glucose,1.5 g/L sodium bicarbonate and supplemented with 2 mM L-glutamax(GIBCO, Invitrogen), 50 μM 2-mercaptoethanol ...
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bioRxiv - Immunology 2022Quote: ... 4.5 g/L glucose,1.5 g/L sodium bicarbonate and supplemented with 2 mM L-glutamax(GIBCO, Invitrogen), 50 μM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: HEK293T human kidney cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; 4.5g/L D-Glucose, L-Glutamine, 110mg/L sodium pyruvate; Thermofisher #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... SW620 cells and murine MC38 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; 4.5g/L D-Glucose, L-Glutamine, 110mg/L sodium pyruvate; Invitrogen #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2020Quote: ... Extracted fungal masses were plunged into a solid medium (7.8 g/L potato dextrose agar [BD], 6 g/L agar [Difco], 100 mg/L Penicillin/Streptomycin [Gibco], and 100 mg/L Kanamycin [Alfa Asear]) and maintained at 28°C for 15 days to screen for possible contaminants and indications of sample viability ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein samples were reconstituted in solvent A (water/ACN [98: 2 v/v] with 0.1% formic acid) and separated using a C18-PepMap column (Thermo Fisher Scientific) with a solvent gradient of 2–100% Buffer B (0.1% formic acid and 98% acetonitrile ...
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bioRxiv - Molecular Biology 2021Quote: ... digests were reconstituted in 10 μL of 0.3% TFA in 2/98 MeCN/H2O and 5 μL were analyzed by LC-MS/MS using an RSLCnano system (Ultimate 3000, Thermo Scientific) interfaced on-line to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... The final transformation mixture contained 10 mM LiAc (Lithium acetate dihydrate, 98%, extra pure, ACROS Organics™, CAS 6108-17-4), 50 µg ssDNA (UltraPure™ Salmon Sperm DNA Solution ...
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bioRxiv - Neuroscience 2022Quote: ... Aliquots of the reaction mix (98 μL) were loaded into each well of a black 96-well plate with a clear bottom (Thermo Scientific) and seeded with 2 μL of diluted brain homogenate ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.4mM L-glutamine (L-glut; Gibco/Invitrogen, Paisley, PA; 25030-081), and
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bioRxiv - Cancer Biology 2020Quote: ... 0.4mM L-glutamine (L-glut; Gibco/Invitrogen, Paisley, PA; 25030-081), and
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bioRxiv - Neuroscience 2020Quote: ... and 146mg/ L of l-lysine D4 (Thermofisher, Waltham, MA, USA) was supplemented for ‘medium’ labelled media and 84mg/L of l-arginine 13C615N4 and 146mg/ L of l-lysine 13C615N4 was added to ‘heavy’ labelled media ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM L-alanyl-L-glutamine dipeptide (Glutamax, Thermo Fisher #35050038), antibiotic antimycotic (Thermo Fisher #15240096) ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 100 mg/L L-cysteine (Fisher Scientific, PA, USA) and 0.1% taurocholate (TCA ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 1% L-alanine-L-glutamine (GlutaMAX, Gibco, Gaithersburg, MD), 50 μg/mL penicillin ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μg/ml streptomycin and 2 mmol/L L-glutamine (Gibco) and used between passages 4 and 12.
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bioRxiv - Immunology 2023Quote: ... L-glutamine (200 mM L-Glutamine, ThermoFisher Scientific Cat. No. 25030149), penicillin-streptomycin (Gibco Penicillin-Streptomycin ...
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bioRxiv - Cell Biology 2021Quote: ... cells were primed in metabolic labeling medium [DMEM containing 4.5 g/L glucose without L-methionine and L-cystine (#21013024, Gibco) including 10% dialysed serum ...
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bioRxiv - Biophysics 2020Quote: Cells were grown in DMEM growth medium ([+] 4.5 g/L D-Glucose, [+] L-Glutamine, [+] 110 mg/L Sodium Pyruvate, Gibco, supplemented with 10 % FBS ...
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bioRxiv - Biophysics 2020Quote: ... they were keep in DMEM growth medium ([+] 4.5 g/L D-Glucose, [+] L-Glutamine, [+] 110 mg/L Sodium Pyruvate, Gibco, supplemented with 10 % FBS ...
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bioRxiv - Biophysics 2020Quote: MCF7-p95ErbB2 cells described previously1 were grown in DMEM growth medium ([+] 4.5 g/L D-Glucose, [+] L-Glutamine, [+] 110 mg/L Sodium Pyruvate, Gibco, supplemented with 10 % FBS ...
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bioRxiv - Genetics 2022Quote: ... and 1x ITS supplement (10 mg/L insulin, 5.5 mg/L transferrin, 6.7 µg/L selenium; Thermo Fisher, 41400045) in DMEM/F-12- PS ...
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bioRxiv - Cancer Biology 2023Quote: ... and MDA-MB-231 TNBC cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; 4.5 g/L D-Glucose, L-Glutamine, 110 mg/L sodium pyruvate; Thermofisher #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... Labeled tau fibrils were subsequently purified from unlabeled dye with using a Zeba 7k MWCO spin desalting column (ThermoFisher Cat# 89882).
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal pS202 and pT205 Tau (AT8, 1:2500, #MN, 1020) and rabbit polyclonal pS262 (1:2000, #44-750G) were from Thermo Fisher. Monoclonal anti-rabbit K-48 linked ubiquitin (1:1000 ...
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bioRxiv - Systems Biology 2020Quote: ... The following primary antibodies were diluted in the blocking solution and applied overnight at 4°C: mouse anti-TAU clone HT7 (1:500, Thermo Scientific), chicken anti-MAP2 (1:10,000 ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing tau were determined using western blot and the pooled samples were quantified with a Micro BCA assay (Thermo Scientific), flash frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Neuroscience 2020Quote: Endogenous tau was immunoprecipitated from whole lysates of differentiated SH-SY5Y cells (1mg) using 10μg of the HT7 tau antibody (ThermoFisher Scientific, #MN1000) and the crosslink magnetic immunoprecipitation kit (Thermo-Pierce ...
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bioRxiv - Neuroscience 2020Quote: ... previously incubated or not for 30 min at room temperature with 5-50 ng/μL anti-mouse tau monoclonal antibody T46 (Thermo Scientific) or anti-human tau monoclonal antibody SP70 (Rockland ...
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bioRxiv - Neuroscience 2021Quote: ... Immunohistochemistry was performed using a primary antibody for tau phosphorylated at serine 202 (mouse monoclonal AT8, 1:1000, Thermo Fisher Scientific). The Mouse on Mouse (MOM ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed with 1X TBS 0.1% Triton-X and incubated in the second primary antibody [phospho-Tau S262 (1:500; Invitrogen 44-750G) Lamp1 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight with an antibody directed against phospho-tau (clone AT8, 1:2000, Thermo Fisher Scientific, Cat. No. MN1020), β-amyloid (clone 4G8 ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was isolated from cultured mouse neurons treated with xcTauOs (250 nM total tau) or vehicle for 6 hours using the mirVana isolation kit (Invitrogen, AM1560). RNA integrity was evaluated by capillary electrophoresis using an Agilent Bioanalyzer ...
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bioRxiv - Genomics 2023Quote: ... the slides were washed 18x2 minutes in TBST and then incubated with primary antibodies at the dilutions indicated after the DAB reaction: IBA1 (1:1000, Rabbit, Wako, 019-19741) and PHF-TAU (1:1000, AT8, Mouse, Thermofisher, MN1020), washed as above and stained with species-appropriate secondary antibodies conjugated to an Alkaline Phosphatase (AP ...
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bioRxiv - Biochemistry 2024Quote: Fluorescent labelling of Tau and 14-3-3ζ protein for microscopy assays was done using amine-reactive DyLight405 or DyLight488-NHS ester (Thermo Scientific) following the manufacturer instructions and the established protocol 34.
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bioRxiv - Cell Biology 2019Quote: ... at 20 nmol/L or control miRNA at 10 nmol/L plus either miR-181a-5p (10 nmol/L; Ambion Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... cat # L-003521-00-0020; siPAK2, Dharmacon, cat # L-003597-00-0020; siPAK4, Dharmacon, cat # L-003615-00-0020; siMAPK1, Thermofisher cat # 4390824 ...
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bioRxiv - Physiology 2024Quote: ... OR3 medium (1 L) consisted of 0.7% w/v powdered Leibovitz L-15 medium with L-glutamine (Thermo Fisher Scientific), 50 mL of 10,000 U penicillin and 10,000 U streptomycin solution in 0.9% NaCl (Nacalai Tesque Inc. ...
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bioRxiv - Cell Biology 2019Quote: ... dissolved in solvent A [0.1% (v/v) FA in water/acetonitrile (ACN) (98:2, v/v)] and quantified by absorbance at 280 nm (NanoDrop, Thermo Fisher Scientific). Samples were analyzed by LC-MS/MS as described in Text S1.
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bioRxiv - Immunology 2023Quote: ... into the reaction mix and incubating it at 37 °C for 15 min and 98 °C for 5 min in the Veriti Thermal Cycler (Applied Biosystems, USA). Quantitative analysis of IL-6 and IFNB expression in THP1 dual reporter cells was performed by StepOne Real-Time PCR Systems (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... highly purified (93-98%) naïve human CD4 T cells were activated using anti-CD3+anti-CD28 Dynabeads (1:1, Thermofisher scientific, cat # 11161D) for 8-24 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were used at 80% to 90% confluency for all the experiments with cell viability ranging from 95 to 98% (Countess II FL, Invitrogen, Thermo Fisher Scientific).