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Citations for Thermo Fisher :
651 - 700 of 7701 citations for TSLPR Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Neuroscience 2020Quote: ... the strips were incubated with goat anti-human IgG Fc HRP-conjugated secondary antibody for 1h (Thermo Fisher Scientific). The film was exposed following routine immunoblotting steps ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed 6x 300μl PBST and developed with an anti-human Fc specific secondary antibody (Invitrogen, Carlsbad, CA) and TMB as described above ...
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bioRxiv - Immunology 2019Quote: ... The plate was incubated with detection antibody (mouse anti-human IgG1 Fc conjugated to horseradish peroxidase clone HP6069; ThermoFisher) for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: The coding sequence of MHV-S Hemagglutinin Esterase inactive (S45A) (1218bp) fused to human IgG-Fc followed by a 6xHis tag in the mammalian expression vector pcDNA3.1(-) (Invitrogen) was kindly provided by Colin Parrish (MHV-S-HE_Fc_His6 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary antibody used was R-Phycoerythrin labelled Goat anti-Human IgG Fc (eBioscienceTM; 12-4998-82, Invitrogen).
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bioRxiv - Microbiology 2023Quote: Human pharyngeal carcinoma Detroit 562 epithelial cells (ATCC_CCL-138) were grown in 10% FCS in alpha MEM media (Gibco). For each set of experiments ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the mixture incubated for 5 min before addition to the shaker flask containing 106 Freestyle 293-F cells/mL grown in suspension in Freestyle 293 expression media (Thermo Fisher). Expression was allowed to continue for 6 days before purification.
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bioRxiv - Immunology 2023Quote: ... and B2(TN99-419) antigens were expressed in FreeStyle 293-F cells by transient transfection in FreeStyle 293 expression media (Thermo Fisher). Cells were co-transfected at a 4:1 ratio of plasmids encoding human metapneumovirus F and furin ...
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bioRxiv - Biochemistry 2022Quote: All recombinant human huntingtin and PRKACA cDNA plasmids were transfected into HEK 293T cells or HTT null HEK 293T cells with Lipofectamine 3000 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... For neutralization assays HEK 293T expressing the SARS-CoV-2 receptor ACE2 (HEK 293T/ACE2) were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Adherent PB2-expressing HEK-293T (HEK-293T-PB2) cells (41) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, #41966029) supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK-Lucia RIGI and HEK-Dual TLR3 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies, Waltham, MA) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Biophysics 2021Quote: ... of Plasmodium falciparum plasmepsin X (XP_001349441) (Fig. 5a) was engineered for expression in a mammalian host (Human Embryonic Kidney cells, HEK cells) by GeneArt (Thermo Fisher, Waltham, MA, USA) and cloned into a variant of pTT5 with the mouse Ig secretion signal peptide on the N terminus and a TEV-cleavable 8x Histidine tag on the C terminus ...
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bioRxiv - Neuroscience 2023Quote: Plasma membrane (PM) fraction was prepared from mouse or human DRG tissues and HEK cells using Mem-PER Plus Kit (Thermo Scientific, Cat No. 89842) and manufacture recommend protocol after biotinylation/streptavidin-magnetic bead (Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... charcoal stripped FCS was used (CS-FCS, Gibco® Life Technologies). The human PCa cell line LAPC4 was kindly provided by Zoran Culig (Innsbruck Medical University ...
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bioRxiv - Cancer Biology 2019Quote: ... charcoal stripped FCS was used (CS-FCS, Gibco® Life Technologies). The human PCa cell line LAPC4 was kindly provided by Zoran Culig (Innsbruck Medical University ...
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bioRxiv - Developmental Biology 2021Quote: HEK-293T cells were cultured in DMEM (Invitrogen) supplemented with 10% heat inactivated FBS (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... HEK-GCaMP6s cells were grown in DMEM:F12 (Invitrogen) containing 5% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK-293T cells were cultured in DMEM (Gibco), supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: HEK-293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK 293FT cells were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: HEK-Blue hNOD1 cells (Invitrogen, hkb-hnod1, USA) were grown in DMEM containing 4.5 g/l glucose ...
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bioRxiv - Biophysics 2022Quote: ... into HEK 293T cells with Lipofectamine 2000 (Gibco). Media was harvested after 48 hours ...
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bioRxiv - Biochemistry 2022Quote: HEK-293A cells (R70507, Life Technologies, Carlsbad, CA) and HEK-293T cells (ATCC ...
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bioRxiv - Genomics 2022Quote: ... HEK 293T was cultured in DMEM (Life Technologies) with 10% FBS (Peak Serum) ...
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bioRxiv - Neuroscience 2019Quote: ... HEK cells were grown in DMEM (GIBCO 11995) containing 10% FBS (Thermo Fisher Scientific A3840101) ...
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bioRxiv - Microbiology 2019Quote: HEK cells were grown in a DMEM (Gibco) containing 10% (vol/vol ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... HEK 293FT cells were obtained from Thermo Scientific. K562 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) ...
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bioRxiv - Cell Biology 2021Quote: HEK 293FT cells were purchased from Thermo Fisher Scientific (cat# R70007 ...
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bioRxiv - Cancer Biology 2022Quote: HEK-293T (ATCC) was grown in DMEM (Gibco) supplemented with 10% FCS (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... HEK 293T (ATCC) were cultured in DMEM (Invitrogen) with 10% FBS and 1% penicillin/streptomycin antibiotics ...
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Long-term precision editing of neural circuits in mammals using engineered gap junction hemichannelsbioRxiv - Neuroscience 2023Quote: HEK 293FT cells were purchased from Thermo Fisher Scientific (cat# R70007 ...
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bioRxiv - Genetics 2023Quote: The HEK-293FT cells (Thermo Fisher Scientific, R70007) were cultured to be 70-90% confluent at the point of transfection to produce lentiviruses ...
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bioRxiv - Biochemistry 2024Quote: ... HEK-293F cells were purchased from Thermo Fisher Scientific company ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T cells were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum and 1% penicillin- streptomycin (Gibco).
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bioRxiv - Cell Biology 2023Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 1% PS ...
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bioRxiv - Genetics 2023Quote: ... HEK-293T cells were grown in DMEM (GIBCO) containing 10% FBS (VWR Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293Ts were maintained in DMEM medium (Gibco) supplemented with 100 U/mL penicillin ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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bioRxiv - Developmental Biology 2022Quote: ... These zona-free eggs were incubated with 200 nM Fc-fusion proteins in Biggers-Whitten-Whittingham (BWW) medium for 1 h and then stained with goat anti-human IgG Fc antibody DyLight 488 (Invitrogen) at a dilution of 1:50 for 1 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using goat anti-Human IgG Fc Secondary Antibody conjugated to horse radish peroxidase (Thermo Fisher Scientific), diluted 1:5000 in blocking solution and incubated for 1 hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated with a 1:10000 dilution (in PBST with 1% BSA) of goat anti-human Fc HRP-conjugated antibody (Invitrogen) at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... and S309 and VHH72-Fc or variants thereof was detected with an AF633 conjugated goat anti-human IgG antibody (Invitrogen). Binding of monomeric VHHs to SARS-CoV-1 or SARS-CoV-2 S was detected with a mouse anti-HisTag antibody (AbD Serotec ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Biochemistry 2021Quote: ... The slide was washed thrice with PBS-T for 1 minute and further incubated with goat anti-human IgG (Fc)-Cy5 (Invitrogen) antibodies at 1:2500 dilution ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific ...