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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for TIM 4 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the mixture incubated for 5 min before addition to the shaker flask containing 106 Freestyle 293-F cells/mL grown in suspension in Freestyle 293 expression media (Thermo Fisher). Expression was allowed to continue for 6 days before purification.
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bioRxiv - Immunology 2023Quote: ... and B2(TN99-419) antigens were expressed in FreeStyle 293-F cells by transient transfection in FreeStyle 293 expression media (Thermo Fisher). Cells were co-transfected at a 4:1 ratio of plasmids encoding human metapneumovirus F and furin ...
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bioRxiv - Biochemistry 2022Quote: All recombinant human huntingtin and PRKACA cDNA plasmids were transfected into HEK 293T cells or HTT null HEK 293T cells with Lipofectamine 3000 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... For neutralization assays HEK 293T expressing the SARS-CoV-2 receptor ACE2 (HEK 293T/ACE2) were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Adherent PB2-expressing HEK-293T (HEK-293T-PB2) cells (41) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, #41966029) supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK-Lucia RIGI and HEK-Dual TLR3 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies, Waltham, MA) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Biophysics 2021Quote: ... of Plasmodium falciparum plasmepsin X (XP_001349441) (Fig. 5a) was engineered for expression in a mammalian host (Human Embryonic Kidney cells, HEK cells) by GeneArt (Thermo Fisher, Waltham, MA, USA) and cloned into a variant of pTT5 with the mouse Ig secretion signal peptide on the N terminus and a TEV-cleavable 8x Histidine tag on the C terminus ...
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bioRxiv - Neuroscience 2023Quote: Plasma membrane (PM) fraction was prepared from mouse or human DRG tissues and HEK cells using Mem-PER Plus Kit (Thermo Scientific, Cat No. 89842) and manufacture recommend protocol after biotinylation/streptavidin-magnetic bead (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: HEK-293T cells were cultured in DMEM (Invitrogen) supplemented with 10% heat inactivated FBS (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... HEK-GCaMP6s cells were grown in DMEM:F12 (Invitrogen) containing 5% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK-293T cells were cultured in DMEM (Gibco), supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: HEK-293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK 293FT cells were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: HEK-Blue hNOD1 cells (Invitrogen, hkb-hnod1, USA) were grown in DMEM containing 4.5 g/l glucose ...
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bioRxiv - Biophysics 2022Quote: ... into HEK 293T cells with Lipofectamine 2000 (Gibco). Media was harvested after 48 hours ...
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bioRxiv - Biochemistry 2022Quote: HEK-293A cells (R70507, Life Technologies, Carlsbad, CA) and HEK-293T cells (ATCC ...
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bioRxiv - Genomics 2022Quote: ... HEK 293T was cultured in DMEM (Life Technologies) with 10% FBS (Peak Serum) ...
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bioRxiv - Neuroscience 2019Quote: ... HEK cells were grown in DMEM (GIBCO 11995) containing 10% FBS (Thermo Fisher Scientific A3840101) ...
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bioRxiv - Microbiology 2019Quote: HEK cells were grown in a DMEM (Gibco) containing 10% (vol/vol ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... HEK 293FT cells were obtained from Thermo Scientific. K562 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) ...
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bioRxiv - Cell Biology 2021Quote: HEK 293FT cells were purchased from Thermo Fisher Scientific (cat# R70007 ...
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bioRxiv - Cancer Biology 2022Quote: HEK-293T (ATCC) was grown in DMEM (Gibco) supplemented with 10% FCS (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... HEK 293T (ATCC) were cultured in DMEM (Invitrogen) with 10% FBS and 1% penicillin/streptomycin antibiotics ...
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Long-term precision editing of neural circuits in mammals using engineered gap junction hemichannelsbioRxiv - Neuroscience 2023Quote: HEK 293FT cells were purchased from Thermo Fisher Scientific (cat# R70007 ...
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bioRxiv - Genetics 2023Quote: The HEK-293FT cells (Thermo Fisher Scientific, R70007) were cultured to be 70-90% confluent at the point of transfection to produce lentiviruses ...
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bioRxiv - Biochemistry 2024Quote: ... HEK-293F cells were purchased from Thermo Fisher Scientific company ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T cells were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum and 1% penicillin- streptomycin (Gibco).
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bioRxiv - Cell Biology 2023Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 1% PS ...
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bioRxiv - Genetics 2023Quote: ... HEK-293T cells were grown in DMEM (GIBCO) containing 10% FBS (VWR Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293Ts were maintained in DMEM medium (Gibco) supplemented with 100 U/mL penicillin ...
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bioRxiv - Biochemistry 2020Quote: pcDNA4-V5-NAA80-M23L was constructed by subcloning NAA80 from pcDNA3.1-NAA80-V525 into the TOPO TA vector pcDNA 4/Xpress-His (Invitrogen). Then the M23L mutation was introduced and the N-terminal Xpress tag was replaced with a V5 tag ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged TpPyShell together with TEV protease was dialyzed overnight at 4°C in SnakeSkin dialysis tube (Thermo Scientific) against dialysis buffer (50 mM Tris-HCl pH8.0 ...
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bioRxiv - Immunology 2020Quote: ... and Luminex (Cytokine Human Magnetic 30 Plex Panel, Thermo Scientific, n=4) per manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Neuroscience 2021Quote: Detection and relative quantification of the prevalence of individual glycolipids were accomplished using the total ion mapping (TIM) and neutral loss scan (NL scan) functionality of the Xcalibur software package version 2.0 (ThermoFisher Scientific) as previously described [18] ...
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bioRxiv - Neuroscience 2022Quote: ... Detection and relative quantification of the prevalence of individual N-glycans was accomplished using the total ion mapping (TIM) functionality of the Xcalibur software package version 2.0 (ThermoFisher Scientific) as previously described (Aoki et al. ...
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bioRxiv - Microbiology 2023Quote: ... CagY complexes were purified using polyclonal anti-CagY antisera (a gift from Dr. Tim Cover) conjugated to Protein G Dynabeads (Invitrogen) isolated by magnetic separation ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
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bioRxiv - Bioengineering 2023Quote: ... The EGFP T-REx 293 cell line used for the mammalian screening was obtained modifying the Flp-In™ T-REx™ 293 Cell Line (Invitrogen) for the expression of inducible EGFP according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... HEK293S GnTl- cells were cultured in Freestyle 293 (GIBCO) supplemented with 2% FBS and 1% Antibiotic-Antimycotic ...
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bioRxiv - Cell Biology 2020Quote: ... A Flp-InTM T-RExTM 293 cell line (Invitrogen) stably carrying a 3xFLAG-IMP1 under a tetracycline inducible CMV promoter was generated and is referred in the text as TREX 293 3xFLAG-IMP1 ...
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bioRxiv - Cancer Biology 2020Quote: ... and GP2-293 cells were cultured in DMEM (Gibco) with 10% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... ExpiFectamine™ 293 transfection kit (A14525, Thermo Fisher Scientific), Opti-MEM™ I reduced serum medium (31985062 ...