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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the bound antibody was detected with goat anti-human Ig (γ chain specific)-HRP conjugated secondary antibody (Thermo Fisher Scientific) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using goat anti-Human IgG Fc Secondary Antibody conjugated to horse radish peroxidase (Thermo Fisher Scientific), diluted 1:5000 in blocking solution and incubated for 1 hr at RT ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... incubated with primary antibody (anti-human ASCT2 from MedImmune diluted 1:3000 in FACS buffer) for 30 min and secondary antibody for 20 min (Goat anti-human IgG Fc PE secondary antibody (Invitrogen) diluted 1:250 in FACS buffer) ...
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bioRxiv - Microbiology 2024Quote: ... immuno-staining with 5 µg/ml anti-spike monoclonal antibody mAb48 (gift of Hugo Mouquet, institute Pasteur) 63 and alexa-Fluor-488-conjugated anti-human IgG antibody (Invitrogen) was performed at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... These recombinant cells expressing individual rod opsin variants were then surface immunostained with a Dylight 550-labeled anti-HA antibody (ThermoFisher) and then fractionated according to the immunostaining intensity of expressed variants using FACS ...
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bioRxiv - Microbiology 2023Quote: ... and intracellular staining was performed using recombinant hu-mouse anti SeV-NP antibody conjugated with AlexaFluor-647 (1:1000) (Invitrogen) in combination with either anti-Krt5 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
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bioRxiv - Microbiology 2020Quote: Mice engrafted with J-Lat cells were treated with recombinant human TNF-α (Cat. #PHC3011, Gibco), a potent and well-known LRA ...
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bioRxiv - Genomics 2022Quote: ... mTESR1 is exchanged with the addition of 5 ng/ml Recombinant Human BMP4 (Fisher Scientific, 314BP010). Exposure to BMP4 triggers differentiation of cells and is considered 0 hours for experimental purposes ...
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bioRxiv - Cell Biology 2022Quote: ... with <0.9% rabbit brain tissue contaminant) and another tube with human recombinant Thromboplastin (Thermo fisher scientific). Next to remove cells ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 12 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) (Thermo Fisher Scientific, USA) was included in the culture medium ...
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bioRxiv - Physiology 2024Quote: ... or 0.75 UI human recombinant insulin/kg body weight for ITTs (catalog no. 12585014, Gibco™). Tail glucose measurements were then taken at the indicated time points in the figures to measure glucose clearance.
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bioRxiv - Neuroscience 2024Quote: iPSCs from all lines were cultured in StemFlexTM medium on human recombinant vitronectin (Thermo Fisher Scientific) as described (34,35,37) ...
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bioRxiv - Immunology 2024Quote: ... recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) (300U/mL; Thermo Fisher Scientific, Waltham, MA) and recombinant human interleukin 4 (rhIL-4 ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and containing recombinant human stem cell factor (h-SCF; Gibco, PeproTech, #300-07; 100 ng/mL) and human IL-6 (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... We simultaneously determined the concentrations of cytokines in supernatant samples using a customized 28-plex immunoassay kit (ProcartaPlex Mouse 28-plex, ThermoFisher Scientific), which employs magnetic beads for detection ...
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bioRxiv - Cell Biology 2024Quote: ... were grown in T25 cm2 flasks at 28 °C or flat bottom well plates at 28 °C + 5 % CO2 in filter- sterilised M199 medium (Life Technologies) supplemented with 2.2 g/L NaHCO3 ...
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bioRxiv - Cell Biology 2023Quote: ... Ovine (Fig. 5) and human Pcells (supplement Fig.5S) were treated with monoclonal mouse SERCA2 antibody (clone 2A7-A1; Thermo Fisher; see supplement) and secondary fluorescent antibody Alexa Fluor® 568 Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies against TSG101 (Invitrogen Clone 4A10, MA1-23296) and PDCD6 (Proteintech ...
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bioRxiv - Cell Biology 2024Quote: ... DCC monoclonal antibody (clone AF5, Thermo Fisher Scientific) from mouse was used for FRAP experiments.
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bioRxiv - Immunology 2024Quote: ... AF488-conjugated Ki-67 antibody (Clone SolA15, Invitrogen). After overnight incubation at 4°C ...
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bioRxiv - Immunology 2024Quote: ... biotin-conjugated CD21/CD35 antibody (Clone 8D9, Invitrogen), AF488-conjugated Ki-67 antibody (Clone SolA15 ...
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bioRxiv - Biochemistry 2020Quote: ... or mouse monoclonal anti-N-Cadherin clone GC-4 followed by AlexaFluor 647-conjugated goat anti-mouse antibody (1:200 dilution, ThermoFisher Scientific). Staining was performed for 30 min at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... The fixed cells were washed three times with PBS and then incubated with primary antibodies, dilution of 1:150 for anti-αSyn (Cloud Clone, #PAB222Hu01) and 1:500 anti-H3 (Invitrogen, # AHO1432) or 1:1000 anti-H2b (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The resulting clones were screened via immunoblotting with gene-specific antibodies anti-NINL rabbit polyclonal antibody (Thermo Fisher Scientific, Waltham, MA,USA) and anti-NIN mouse monoclonal antibody (LSBio ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescein (FITC)-conjugated mouse monoclonal anti-CD14 antibody (clone TuK4, Life technologies, Thermofisher Scientific, Carlsbad, CA, USA), allophycocyanin (APC)-conjugated mouse monoclonal anti-CD16 antibody (clone 3G8 ...
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bioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and then stained with anti-murine ProIL-1β antibody (clone NJTEN3, Thermofisher), washed and analyzed ...
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bioRxiv - Physiology 2022Quote: ... Diluted primary antibody (100 µL of 1:200 rat anti-rabbit a-tubulin, Clone YL1 / 2, Invitrogen) was pipetted onto the Parafilm and the coverslips were placed cell-side down on top of the solution and incubated at 4 °C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [Thermofisher] ...
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bioRxiv - Immunology 2021Quote: DNA constructs encoding anti-SCARB2 antibody heavy and light chain variable domains (clone JL278) were synthesized (ThermoFisher) and cloned into mammalian expression vectors containing a mouse IGHV signal peptide and IgG1 constant regions ...
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bioRxiv - Microbiology 2023Quote: ... and stained with FITC-conjugated anti-SIV Gag antibody (clone 552F12) in Perm/Fix Medium B (Invitrogen). After washing ...
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bioRxiv - Cell Biology 2024Quote: ... Other primary antibodies used for immunofluorescence were mouse anti-acetylated tubulin (clone 6-11B, 32270, Thermo Fisher) at 1:10000 ...
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bioRxiv - Microbiology 2021Quote: The 17,384 Ultimate™ ORF LITE clones (Human collection) were obtained from ThermoFisher scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... with gating for GFP+ human CD45+ cells (clone 2D1, 17-9459-42, ThermoFisher). Immunohistochemistry analysis on liver sections was performed as described in the section below ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with corresponding secondary antibodies for 1 hr at room temperature: goat anti-human-Alexa-488 and goat anti-mouse-Alexa-488 (Invitrogen) for 293T internalization studies and goat anti-rabbit-Alexa-488 and goat anti-human-Alexa-555 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated for 1 hour with secondary antibodies (1:500 goat anti-human Alexa-fluor 647 [Invitrogen #A48279TR], 1:500 goat anti-rabbit Alexa-fluor 594 [Invitrogen #A32740] ...
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bioRxiv - Immunology 2024Quote: ... and functional grade anti-CD3 (aCD3, 1 μg/mL) and anti-CD28 (aCD28, 1 μg/mL) murine or human monoclonal antibodies (Invitrogen)) ...
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bioRxiv - Immunology 2024Quote: ... and functional grade anti-CD3 (aCD3, 1 μg/mL) and anti-CD28 (aCD28, 1 μg/mL) murine or human monoclonal antibodies (Invitrogen). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... the cells were stained with fluorescently labelled secondary antibodies (goat anti-human IgG Dylight488 from Invitrogen; catalogue number SA5-10134] and goat anti-mouse AlexaFluor647 from Invitrogen; catalogue number A32728] ...
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bioRxiv - Microbiology 2024Quote: ... Colon tissue sections were prepared for immunofluorescence staining as previously described [27] using goat anti-human-AF647 and rabbit anti-GFP-AF488 conjugated antibodies (ThermoFisher, Invitrogen).
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bioRxiv - Immunology 2020Quote: ... 0.05% Tween-20 (PBST) and ALP-conjugated goat anti-human IgG (H+L) antibody (Thermo Fisher) was added into each well and incubated at 37°C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... 50μL of polyclonal anti-human β2M HRP-conjugated IgG detection antibody raised in rabbits (ThermoFisher Scientific), diluted 1:2500 in 2% BSA (0.2μg/mL) ...
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bioRxiv - Microbiology 2021Quote: ... Alexa488- or Rhodamine-conjugated goat anti-human secondary antibody was added (1/500 dilution) (Thermo Fisher), and incubated for 2~3 hr at room temperature in the dark ...
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bioRxiv - Microbiology 2021Quote: ... R-phycoerythrin (PE) conjugated goat anti-human IgG Fc secondary antibody (Invitrogen, Cat#12-4998-82) and PE conjugated mouse anti-PSGL-1 mAb (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... further washed and then detected by an anti – human IgG secondary antibody labelled with HRP (Invitrogen), and absorbance detected at 450nm on a spectrophotometer (Wallac).