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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Heme oxygenase 1 HO 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... arrays were incubated for 1h30 with AF647-conjugated goat anti-human IgG antibodies (at 1 µg/ml in PBS; Thermo Fisher Scientific), and revealed using GenePix 4000B microarray scanner (Molecular Devices ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated 20 min at 4°C with AF647-conjugated goat anti-human IgG antibodies (1:1000 dilution; Thermo Fisher Scientific) and LIVE/DEAD Fixable Viability dye Aqua (1:1000 dilution ...
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bioRxiv - Immunology 2022Quote: FLAG-DNAM-1-expressing human Treg cells were co-cultured in complete RPMI-1640 culture medium with 2 × 104 CellTrace Violet (Thermo Fisher Scientific)-labeled Tconv cells (freshly isolated from the same donor as that of the Treg cells ...
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bioRxiv - Microbiology 2020Quote: ... They were then incubated with 1:200 diluted Alexa Fluor 594 conjugated goat anti-human IgG secondary antibody (Invitrogen, Thermo Fisher Scientific) for 45 min at RT and subjected to flow cytometric analysis with a CytoFLEX flow cytometer (Beckman) ...
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bioRxiv - Cell Biology 2019Quote: All human cells (listed in table 1) were cultured at 37°C in an atmosphere of 5% CO2 in DMEM (Thermo Fisher Scientific) supplemented with penicillin/streptomycin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Systems Biology 2022Quote: The human monocytic cell line THP-1 (LGC, ATCC-TIB-202) was cultured and expanded in RPMI 1640 media (Gibco, 61870-010) supplemented with 10% FCS (BioConcept ...
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bioRxiv - Physiology 2024Quote: ... at an effector/target ratio of 2:1 in the presence of mouse anti-human CD107a-FITC (11-1079-42; Thermo Fisher Scientific). Cocultures between CTLs and EndoC-βH1 cells expressing HLA-A2 were performed at 37 °C for 1.5 h in Iscove’s Modified Dlbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in 1 ml medium per well of a 24-well plate and stimulated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific), IL-7 (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed two times with FACS buffer to remove any nonbounded Fc-hDectin1 and resuspended in FACS buffer containing a 1:200 dilution of the secondary antibody F(ab’)2-Goat anti-Human IgG conjugated to AlexaFluor™ 488 (ThermoFisher Scientific). This solution was incubated for 1 hour on ice before finally washing twice with FACS buffer and measuring the fluorescence intensity of 5 000 cells using a Guava® flow cytometer with 488nm excitation and fluorescence emission selected using a 522/30 bandpass filter.
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bioRxiv - Physiology 2023Quote: Immortalized human microvascular endothelial cells (HMEC-1) purchased from ATCC (Manassas, VA) were cultured in antibiotic-free MCDB131 (Life Technologies, Carlsbad, CA) supplemented with 10 mM Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... 0.5-1 µg of total RNA was isolated from human islets and reverse transcribed using an M-MLV RT kit (Invitrogen, MA, USA). TaqMan primers (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: ... To create SiRPα-Fc fusion soluble proteins the cDNA sequence of the full extracellular domain (aa 27-371) of human wild-type allele 1 SiRPα (wtSiRPα) cDNA (Uniprot: P78324) was codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific), fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1 ...
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bioRxiv - Microbiology 2023Quote: ... The pooled libraries were mixed with virus-specific 161 biotinylated probes in the presence of human Cot-1 DNA (Invitrogen, Cat#15279011) and xGen Universal Blocking Oligos (IDT ...
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bioRxiv - Cancer Biology 2022Quote: ... while the human pancreatic cancer cell line AsPC-1 (passage 11) was cultured in RPMI-1640 medium (L-glutamine supplement; Gibco, Thermo Fisher Scientific), both supplemented with 10 % (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times with PBS and labeled with secondary antibodies (goat anti-rabbit/human conjugated to Alexa Fluor 647; 1:1000; Invitrogen, #A21246/A21445) and FluoTag-X4 anti-GFP conjugated to Atto488 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
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bioRxiv - Genetics 2023Quote: ... cells were stimulated with Dynabeads Human T-Activator αCD3/αCD28 at a bead-to-cell ratio of 1:2 (11131D, Thermo Fisher Scientific). Cell counts were determined every 2-3 days ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and a 1:1000 dilution of goat anti-human IgG (γ-chain specific) alkaline phosphate conjugate antibody (A3187, Thermo Fisher) was added and incubated for 1 h ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed three times with PBS and incubated with CD15 (C3D-1) mouse anti-human monoclonal antibody (Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... one million human iPSCs were thawed and resuspended with 1 mL of Dulbecco’s Modified Eagle Medium F12 (DMEM F12) media (Thermofisher, Catalog No. 12634010) then diluted in 2 mL of warm DMEM F12 in a 15 mL Eppendorf conical tube (Eppendorf ...
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bioRxiv - Neuroscience 2024Quote: ... Phosphorylation was induced by incubating 1 µg of recombinant human polo-like kinase 3 (PLK3) protein (Thermo Fisher Scientific, MA, USA; #PR7316B) with 50 µL of mouse WT α-synuclein in kinase reaction buffer for 8 hrs at 30°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The constructs were used for in vitro transcription and translation using the 1-Step Human High-Yield Mini IVT Kit (Thermo Fisher; 88891) according to the manufacturer’s instruction ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Human slices were routinely stained with anti-NeuN (diluted 1:300 from stock; Rabbit anti-NeuN, Thermo Fisher Scientific PA5-37407, RRID:AB_2554049), visualized with AF488-conjugated anti-rabbit secondary antibody (1:300 from stock ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 mL complete human medium (DMEM/F12 [Gibco, Massachusetts, US], 10% FBS [PAA Cell Culture Company, UK], 1% GSP [Gibco, Massachusetts, US]). All cultures were maintained at 37 °C/5% CO2 in a humidified incubator ...
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bioRxiv - Microbiology 2024Quote: ... Upon stabilisation of baseline readings for human plasma experiments plasma was diluted 1:40 in media (RPMI [Thermo Fisher Scientific, U.S.A.] with B27 Supplement [Thermo Fisher Scientific, U.S.A.]), then transferred to the hiPSC-derived cardiomyocytes for 48 hours of treatment either in the presence or absence of 100ng/mL of dexamethasone (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Program T-023 with 150 pmol/106 cells of human TSP-1 and TSP-4-specific siRNAs (#s14108 and #s14100, Thermo Fisher Scientific) and control scrambled siRNAs (#4390846 ...
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bioRxiv - Bioengineering 2019Quote: ... mouse anti-zona occludens-1 (ZO-1) (Invitrogen 1:100, Cat #33-9100), anti-βIII-tubulin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and 1% penicillin-streptomycin (100 UI.ml-1 and 100 mg.ml-1, Gibco, Invitrogen), and maintained at 37°C in a humidified incubator with 5 % CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... and 1% penicillin-streptomycin (100 UI.ml-1 and 100 mg.ml-1, Gibco, Invitrogen), and maintained at 37°C in a humidified incubator with 5 % CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... 555 and 488 (Life Technologies, 1:400, 1:400 and 1:200 respectively); goat anti-mouse Alexa 594 ...
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bioRxiv - Cell Biology 2021Quote: ... 555 and 488 (Life Technologies, 1:400, 1:400 and 1: 200 respectively) goat anti-mouse HRP (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... 555 and 488 (Life Technologies, 1:400, 1:400 and 1:200 respectively); goat anti-mouse Alexa 594 ...
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bioRxiv - Cell Biology 2021Quote: ... 555 and 488 (Life Technologies, 1:400, 1:400 and 1: 200 respectively) goat anti-mouse HRP (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... hygromycin B (0.25 mg mL−1) and G418 (1 mg mL-1, Invitrogen). HeLa cells were cultured at 37°C in a 5% CO2-containing atmosphere ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-Vimentin (Invitrogen, V9, WB 1:1000-1:2500, IF 1:100), mouse anti-Actin (NeoMarkers ...
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bioRxiv - Neuroscience 2023Quote: ... or mouse anti- zonula occludens 1 (ZO-1) (1:100, Invitrogen, 35-2500). Mouse anti-ZO-1 was conjugated beforehand with CF488A using the Mix-n-Stain™ CF™ 488A Antibody Libelling Kit (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Cell Biology 2023Quote: ... incubated in secondary antibody diluted in 1% milk with 1% BSA (1:5000, Invitrogen G21234; 1:5000, Invitrogen, 31430) for 1 hour at RT ...
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bioRxiv - Cell Biology 2023Quote: ... incubated in secondary antibody diluted in 1% milk with 1% BSA (1:5000, Invitrogen G21234; 1:5000, Invitrogen, 31430) for 1 hour at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-PDIA3 (AMAB90988; WB, 1:3000; IF, 1:500), RanGAP1 mouse (33-0800; IF, 1:300; PLA, 1:500) was from Invitrogen. Anti-VAPB (66191-1-1g ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were then incubated with primary antibody (anti-4HNE from Millipore 1:1000; anti-DNP 1:500; anti-phospho-NFκB from Invitrogen 1:1000; anti-NFκB from Invitrogen 1:1000) at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... mutant and control cells were resuspended 1:1 in 1 mL of serum-free DMEM containing 1:20000 Hoechst33342 dye (ThermoFisher), and loaded on a VyCAP chip (VyCAP B.V ...
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bioRxiv - Cell Biology 2022Quote: ... in serum-free N2B27 medium (DMEM/F12 [Gibco] and Neurobasal [Gibco] 1:1 ratio, 1:100 N2 Supplement [Gibco], 1:50 B27 Supplement [Gibco], 2 mM Glutamax [Gibco] ...
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bioRxiv - Cell Biology 2022Quote: ... in serum-free N2B27 medium (DMEM/F12 [Gibco] and Neurobasal [Gibco] 1:1 ratio, 1:100 N2 Supplement [Gibco], 1:50 B27 Supplement [Gibco] ...
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bioRxiv - Developmental Biology 2022Quote: ... and Neurobasal in 1:1 ratio [Gibco 21103-049], with 1:200 N2 Supplement [Gibco 17502-048], and 1:100 B27 Supplement [Gibco 17504-044] ...