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Citations for Thermo Fisher :
651 - 700 of 5395 citations for Ferritin heavy chain FTH1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... recombination cassettes were generated from the pEPKanS template by polymerase chain reaction (PCR) with Phusion High Fidelity DNA polymerase (Thermo Fisher Scientific) using long oligonucleotides (Ultramers ...
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bioRxiv - Molecular Biology 2022Quote: ... and YrbA open reading frames (ORFs) were generated by polymerase chain reaction (PCR) and AccuPrime Taq DNA Polymerase (Thermo Fisher Scientific, USA). The sequences of the primers used for PCR are shown in Table S1 ...
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bioRxiv - Genetics 2022Quote: ... The SARS-CoV-2 infection was screened and confirmed by at least one quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) assay with the Applied Biosystems 7500 Fast RT-PCR System (Thermo Fisher Scientific), using a protocol previously described for the detection of 2019 novel Coronavirus (2019-nCoV ...
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bioRxiv - Physiology 2022Quote: Relative gene expression in undifferentiated cells and 21 day ALI differentiated monolayers was assessed using quantitative reverse transcription–polymerase chain reaction (qRT-PCR) with the SuperScript III Platinum SYBRGreen OneStep qPCR Kit (Life Technologies, UK) and an AriaMX real-time thermal cycler ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). cDNA was amplified using the Power SYBR Green PCR Master Mix (catalog #4367659 ...
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bioRxiv - Developmental Biology 2022Quote: ... Gene expression was assessed with real-time quantitative polymerase chain reaction (qPCR) using SYBRTM Green PCR Master Mix (Thermo Fisher Scientific, MA) on a Roche LightCycler 480 II (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The 25 μL of polymerase chain reaction of each reaction included 12.5 μL of Master Mix AmpliTaq Gold™ 360 (Applied Biosystems, USA), 20 mM of each primer and 6 μL od DNAse/RNAse free water ...
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bioRxiv - Molecular Biology 2023Quote: The expression plasmids encoding the heavy and light chains of 8ANC195 (expression plasmids kindly provided by Michel Nussenzweig, the Rockefeller University) were transiently transfected into Expi293F cells (Thermo Fisher Scientific) using ExpiFectamine 293 transfection as described (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... was recombinantly overproduced together with the myosin regulatory (Sqh) and essential light chain (Mlc-c) in the baculovirus/Sf9 insect cell system (Thermo Fisher Scientific) and prepared as described (Heissler et al. ...
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bioRxiv - Biophysics 2023Quote: RBL-2H3 cells stably expressing clathrin light chain A tagged with green fluorescent proteins were cultured in minimum essential medium (MEM) (Invitrogen 11095-09) with 20% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... Lysates were boiled for 5 minutes (except lysates used for detection of electron transport chain complexes) and protein content was determined using a BCA kit (23225, Thermo Fisher Scientific). Proteins were separated by SDS-PAGE and transferred to a 45 µm PVDF membrane ...
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bioRxiv - Microbiology 2023Quote: ... the recombination cassette was generated from the pEPKanS template by polymerase chain reaction (PCR) with Phusion High Fidelity DNA polymerase (Thermo Fisher Scientific) using long oligonucleotides (Ultramers ...
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bioRxiv - Microbiology 2023Quote: ... real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using Taqman probe (Mm00458221 ...
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bioRxiv - Microbiology 2023Quote: ... to be separated on 8% SDS-polyacrylamide gel electrophoresis (PAGE) or subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using Superscript IV (Invitrogen Life Technologies), to create cDNA and then DNA to amplify for sequencing.
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bioRxiv - Microbiology 2023Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Cancer Biology 2022Quote: ... real-time quantitative polymerase chain reaction (RT-qPCR −60°C annealing temperature) was performed with Power SYBR Green PCR Master Mix (Applied Biosystems; 4367659). Assays were run in triplicates on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Physiology 2023Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed on a StepOne Plus real-time PCR system (Applied Biosystems, Foster City, CA) using PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: ... and light chains) was mixed with 45 µL of 40 kDa polyethylenimine (1 mg/mL) and 3 mL of F17 media (Thermo Fisher, A1383501) in a sterile hood for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time reverse transcription polymerase chain reactions (RT-PCRs) were performed on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Life Technologies). Data were normalized to housekeeping genes (Tubulin ...
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bioRxiv - Immunology 2023Quote: ... to amplify immunoglobulin heavy and light chain variable regions and yielding variable regions were confirmed by using 96 well E-gel agarose electrophoresis (Thermo Fisher Scientific). Purified PCR amplicons were then sequenced with forward and reverse primers and contigs of the amplified immunoglobulin sequences were made by the Duke automated sequence analysis pipeline (Duke ASAP) ...
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bioRxiv - Biophysics 2023Quote: Cloning and mutagenesis in this work were carried by Polymerase Chain Reaction (PCR) with Phusion Hot Start II DNA Polymerase (Thermo Fisher Scientific). For initial protein characterization ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse-transcriptase real-time quantitative polymerase chain reaction (RT-qPCR) was carried out with TaqMan® Gene Expression Assays (Thermo Fisher Scientific) in complementary deoxyribonucleic acid (cDNA ...
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bioRxiv - Microbiology 2023Quote: Chip-based digital polymerase chain reaction (c-dPCR) was performed with the QuantStudio™ 3D Digital PCR System (User Guide, Catalog Number A29154, Thermo Scientific). Each c-dPCR reaction was prepared in a final volume of 15,5 µL containing 5 µL QuantStudio™ 3D Digital PCR Master Mix v2 (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... comprising the variable heavy chain and light chains joined with the 15-residue linker was transiently expressed in a secreted form using FreeStyle™ 293-F cells (Thermo Fisher) in FreeStyle™ F17 Expression Medium supplemented with L-glutamine and 1x MEM non-essential amino acids (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed using the Applied Biosystems POWER SYBR Green Master Mix (Applied Biosystems, #4367659). All samples were run in biological triplicates ...
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bioRxiv - Cell Biology 2024Quote: A dual system was developed to trace both 0,57 mg/mL FITC-Dextran and 2 μM of the long-chain fatty acid analogue BODIPY-C12 (558/568) (ThermoFisher, cat. #D3835) in one well ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Quantitative polymerase chain reaction (qPCR) analysis was performed using a QuantStudio 12 K Flex real-time PCR system (Applied Biosystems, United States) and Luminaris color probe qPCR master mixes (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Conventional two step reverse transcriptase polymerase chain reaction (RT-PCR) was employed using the Invitrogen SuperScript IV first-strand synthesis system (Thermo Scientific, USA) for cDNA synthesis and DreamTaq Green PCR Master Mix (2X ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time quantitative polymerase chain reaction (RT-qPCR) was performed with AmpliTaq Gold polymerase in an Applied Biosystems 7500 thermocycler (Thermo Fisher Scientific). Primer sequences are available upon request.
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis was performed by using the ViiA7 or QuantStudio Real-time PCR system (Thermo Fisher Scientific) using TaqMan Gene Expression Assays (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: Human neural stem cells derived from H9 (WA09) human embryonic stem cells were purchased from Thermo Fisher. The cells were cultured in complete StemPro NSC SFM (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: NSChIPS (Human Neural Stem Cells derived from the human induced pluripotent stem (iPS) cell line) (ThermoFisher, #A3890101)
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bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
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bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Neuroscience 2020Quote: Levels of total APOE protein were analysed by Human ELISA Kit from Invitrogen (Apolipoprotein E Human ELISA Kit). Levels of Aβ were analysed by ELISA (Human β Amyloid 42 ELISA Kit Wako high sensitive #298-64401 and Human β Amyloid 40 ELISA Kit Wako #298-64601 ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for human cells are mouse anti-human CD3 conjugated to APC (Thermo Fisher, 47-0036-42), mouse anti-human CD8 conjugated to FITC (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... where EGF and noggin were replaced with human forms with 10 ng ml−1 human EGF (Invitrogen, PHG0311), 100 ng ml−1 human noggin (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human TNF-α (hTNF-α)-PE and anti-human IFN-ɣ (hIFN-ɣ)-PECy7 were from ThermoFisher. Anti-human CD4 (hCD4)-BV711 was from Biolegend.
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...