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Citations for Thermo Fisher :
651 - 700 of 1712 citations for Bordetella Pertussis Toxin Frozen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The grids were frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher).
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bioRxiv - Biochemistry 2023Quote: ... and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
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Effects of menopause and high fat diet on metabolic outcomes in a mouse model of Alzheimer’s diseasebioRxiv - Neuroscience 2023Quote: ... then frozen in optimal cutting temperature (O.C.T.) solution (23-730-571, Thermo Fisher Scientific) and stored at -80°C until further processing ...
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bioRxiv - Neuroscience 2023Quote: ... freshly frozen brains were cryosectioned (20 μm) onto Superfrost Plus glass slides (Fisher Scientific) and stored at −80°C ...
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bioRxiv - Immunology 2024Quote: ... tumors were frozen in Tissue-Tek Optimal Cutting Temperature (O.C.T) compound (Sakura, Fisher Scientific) and 5 µm thick sections were mounted on polypropylene coated glass slides (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... Frozen flies were homogenized in 120 µl of TRI reagent (MRC, Thermo Fisher Scientific) using a plastic pestle ...
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bioRxiv - Biophysics 2024Quote: ... and flash-frozen in liquid ethane using a Vitrobot Mark III (Thermo Fisher Scientific) operated at 4 °C and 100% humidity ...
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bioRxiv - Neuroscience 2024Quote: ... Fixed brain tissues were cut and frozen with a microtome (HM525 NX, Thermo Fisher) in coronal sections (40 µm) ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was isolated from frozen pellets and washed using a modified Trizol (Invitrogen) extraction protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA was extracted from frozen endosperm samples via RNAqueous Total RNA Isolation Kit (Invitrogen) according to the kit protocol ...
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bioRxiv - Neuroscience 2024Quote: Frozen samples were homogenized in 1000μl TRIzol Reagent (15596026, Thermo Fisher Scientific, Waltham, MA) and vortexed at 2000 rpm for 5 min ...
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bioRxiv - Cell Biology 2024Quote: Plunge frozen grids containing bacterial samples were clipped into Cryo-FIB AutoGrids (ThermoFisher Scientific). Clipped grids were loaded onto the stage of an Aquilos 2 FIB-SEM (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... and plunge-frozen into liquid ethane by a Vitrobot Mark IV (Thermo Fisher Scientific) using the following parameters ...
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bioRxiv - Biophysics 2024Quote: ... and plunge-frozen in liquid ethane using the Vitrobot Mark IV (Thermo Fisher Scientific). The following parameters were used ...
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bioRxiv - Biophysics 2024Quote: Frozen kinetochore samples were imaged on a 300 kV Titan Krios (ThermoFisher Scientific, MA) equipped with a high-brightness Field Emission Gun (x-FEG) ...
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bioRxiv - Neuroscience 2021Quote: ... pairwise injections of 80 nL of 0.5% (wt/vol) fluorescently labeled cholera toxin B subunit (CTB-488, ThermoFisher C22841, CTB-647, ThermoFisher C34788). In C57BL6/J mice ...
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bioRxiv - Neuroscience 2020Quote: Mice used for retrograde tracing experiments received cholera toxin b subunit (CTB) conjugated with Alexa Fluor 488 and/or Alexa Fluor 647 (CTB-488 and CTB-647; ThermoFisher, C34775 and C34778).
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bioRxiv - Microbiology 2021Quote: ... 50 μl PCR reactions were carried out using 10 ng of sample DNA template and toxin-specific gene primers (Table 3) along with Platinum™ Taq Polymerase (Invitrogen/ThermoFisher, Carlsbad, CA) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2020Quote: ... the left hemisphere VMH was pressure injected with 180 nl of a bi-directional neural tracer cocktail consisting of 5 μg/μl retrograde Cholera toxin subunit B conjugated with Alexa Fluor 488 (Life Technologies, cat# C22841) and 50 μg/μl anterograde dextran amine 10,000 MW conjugated with Alexa Fluor 488 (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The injections were either a fluorescent dextran amine or a recombinant cholera toxin subunit B tracer: (Dextran-405; CTB-488, CTB-594, CTB-647, Invitrogen #C22841, #C34777, #C34778). We chose our injection volume based on the size ...
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bioRxiv - Neuroscience 2023Quote: Animals that underwent optic nerve crush surgeries were intravitreally injected on the surgical eye with 2 µl of Alexa 594-conjugated-cholera toxin β (Life technologies #C22842) at day 14 ...
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bioRxiv - Neuroscience 2023Quote: ... A small hole was made in the eye using a sterile 34-gauge needle and ∼0.5 μl of cholera toxin subunit B conjugated with Alexa Fluor 488 (CTB-488, ThermoFisher Scientific, Catalogue Number: C34775) diluted in 0.9% sterile saline was intravitreally pressure-injected into the right eye using a pulled-glass micropipette coupled to a Picospritzer (Parker Hannifin).
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bioRxiv - Immunology 2024Quote: ... Quantification of the Alexa Fluor® 647 ratio per tetanus toxin was obtained by scanning measurements using the NanoDrop One c device (Thermo Fisher Scientific) with 280 and 650 nm readings ...
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bioRxiv - Bioengineering 2024Quote: ... were grown in DMEM with 10% FBS and 1% P/S and selected with 1 ug/mL of diphtheria toxin (Cayman Biologics, Ann Arbor, MI) and 300 ug/mL hygromycin (ThermoFisher Scientific, Waltham, MA). Selection for transgene-positive cells was confirmed by flow cytometry for mouse Lyt2 expression as a reporter gene (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: Flash frozen tumor pieces were homogenized using Fisher Homogenizer 150 (Thermo Fisher, Waltham, MA, USA). Total RNA was isolated using QIAGEN RNeasy Midi Kit (#75142 ...
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bioRxiv - Cancer Biology 2021Quote: Flash frozen tumor pieces were homogenized using Fisher Homogenizer 150 (Thermo Fisher, Waltham, MA, USA). Total RNA was isolated using QIAGEN RNeasy Midi Kit (Hilden ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated from deep-frozen plant material using the TRIzol® method (Invitrogen) according to the manufacturer’s specifications ...
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bioRxiv - Genetics 2021Quote: RNA was extracted from frozen tissues following standard Trizol chloroform protocol (Invitrogen, cat. number 15596026) and aqueous fractions were then taken directly into Qiagen RNA plus extraction kit (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: ... Frozen sections (10μm) were placed onto SUPERFROST PLUS slides (Thermo Fisher Scientific, Waltham, MA, USA) and used for immunofluorescence.
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bioRxiv - Cell Biology 2022Quote: ... The frozen block was cut in 5-μm-thick using Cryostar NX70 (Thermo Fisher Scientific) and fixed in 10% formalin in 0.1 M phosphate buffer pH7.4 for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: Tissue was collected and immediately frozen on dry ice in 100 μL Trizol (Life Technologies) before RNA extraction ...
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bioRxiv - Physiology 2019Quote: ... colon and ileum] were collected and frozen at −80°C before homogenization in Trizol (Invitrogen). RNA was isolated according to the manufacturer’s protocol (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... 5 x 107 frozen macrophages were resuspended in three ml of TRI-Reagent (Invitrogen AM9738) and vortexed for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... DNA was extracted from frozen donor retinal tissue (PureLink Genomic DNA Kit, ThermoFisher, Waltham, MA), amplified by PCR using specific primers before genotyping (Quintara ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was extracted from frozen tumour tissue with the mirVana miRNA Isolation Kit (Ambion), as described previously [31] ...
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bioRxiv - Biochemistry 2020Quote: ... and plunge frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA). Grid quality was examined using JEM-2100F (JEOL ...
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bioRxiv - Molecular Biology 2019Quote: ... Grids were plunge-frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific) with a blotting time of 2 s at 10°C and 100% humidity.
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bioRxiv - Neuroscience 2019Quote: Freshly dissected mouse brains were quickly frozen in a 2-methylbutane (Fisher Scientific #O3551-4) dry ice bath ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted total RNA from frozen tissue using a standard TRIzol® (Thermo Fisher Scientific) extraction protocol (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/trizol_reagent.pdf) ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was isolated from frozen parasite-infected blood pellets using a modified Trizol (Invitrogen) extraction protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Grids were immediately plunge-frozen in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific), and excess protein was blotted away by using grade 595 filter paper (Ted Pella ...
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bioRxiv - Cancer Biology 2020Quote: ... snap frozen in liquid nitrogen immediately after harvesting and stored in RNAlater (Cat. AM7021, Ambion) at -80°C ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... Grids were immediately plunge-frozen in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific), and excess protein was blotted away by using grade 595 filter paper (Ted Pella ...
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bioRxiv - Biochemistry 2020Quote: ... and flash frozen in liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher Scientific) and a liquid-ethane cryostat set to −180 °C (Russo et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Grids were then flash-frozen in liquid ethane using an FEI Vitrobot (Thermo Fisher Scientific). Data collection was performed at the Umeå University Core Facility for Electron Microscopy (UCEM ...
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bioRxiv - Cell Biology 2021Quote: Specimens were randomly selected from each vial population and frozen in 1 mL RNAlater (ThermoFisher). Preliminary RNAseq specimens contained one fly per 1 ml RNAlater ...
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bioRxiv - Molecular Biology 2021Quote: Snap frozen skin biopsies were thawed in wet ice and processed using TRIzol Reagent (Ambion, Thermo Fisher Scientific Inc. ...
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bioRxiv - Physiology 2021Quote: ... Frozen aliquots were thawed on ice and analyzed using a Leptin Rat ELISA kit (Invitrogen).
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bioRxiv - Microbiology 2020Quote: Frozen grids were first screened on a Glacios microscope operating at 200 kV (Thermo Fisher) before imaging on a Titan Krios G2 (Thermo Fischer ...