Labshake search
Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... 4°C and lysed using 400 µL B-PER bacterial protein extraction reagent (Thermo Scientific, MA, USA) according to the instructions of the manufacturer ...
-
bioRxiv - Plant Biology 2022Quote: ... The seedlings were screened by microscopy for YFP and then fixed and processed for immunofluorescence microscopy using a 1:2000 dilution of monoclonal anti-α-tubulin B-5-1-2 antibody (Life Technologies; 32-2500) followed by 1:2000 dilution of Alexa-568 goat anti-mouse antibody (Thermo Fisher ...
-
bioRxiv - Biochemistry 2020Quote: ... 4 μL of 10% of trifluoroacetic acid (Thermo Fisher Scientific, Inc.) was added to the digested mixture and incubated at 37 °C for 30min ...
-
bioRxiv - Developmental Biology 2022Quote: Cells were fixed for 10 minutes with 4% paraformaldehyde (Thermo Scientific), permeabilized for 10 minutes with 0.5% Triton X-100 (Sigma) ...
-
bioRxiv - Physiology 2021Quote: ... lysotracker red (LTR; 100nM) and Fluo-4 (10 μM) (Life Technologies), and Alexa-488-phalloidin and Rhodamine-phalloidin (Invitrogen) ...
-
bioRxiv - Immunology 2020Quote: ... Proteins were separated on a BisTris 4-10% Gradient gel (Invitrogen) and the samples run alongside a protein standard (Biorad) ...
-
bioRxiv - Cell Biology 2021Quote: ... and run on 4-12 % or 10% NuPAGE gel (Thermofisher Scientific) for immunoblotting with respond antibodies ...
-
bioRxiv - Biochemistry 2022Quote: ... supplemented with 10% FBS and 4 mM GlutaMAX (Thermo Fisher Scientific). Images were acquired as a series of 0.6 µm z-stacks with a Plan Apo 40x/0.95 Corr (DIC N2 / 40X I ...
-
bioRxiv - Immunology 2023Quote: ... and 10 ng/ml IL-4 (Preprotech, Thermo Fisher Scientific, USA) 17 ...
-
bioRxiv - Cancer Biology 2023Quote: ... organoids were fixed in 10% formalin (Thermo Fisher Scientific, SF100-4) for 1 h ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... 2.5 × 10-4 U DNase I (ThermoFisher Scientific, Waltham, MA USA) and 1 U DNA polymerase I (ThermoFisher Scientific) ...
-
bioRxiv - Developmental Biology 2023Quote: ... 4 µl 10 mg/ml salmon sperm DNA (Invitrogen, 15632-011) in the ChIP dilution buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were fixed for 10 minutes with 4% paraformaldehyde (Thermo Fisher) then permeabilized for 10 minutes in PBS with 0.25% Triton-X100 (Thermo Fisher) ...
-
bioRxiv - Neuroscience 2024Quote: ... in HBSS or Fluo-4 AM at 10 μM in (ThermoFisher) 2% Pluronic F-127 (ThermoFisher ...
-
bioRxiv - Immunology 2024Quote: ... 100 nM 4-OHT and 10% Fetal Bovine Serum (10082147, GIBCO). For Hoxb8 progenitor cell differentiation ...
-
bioRxiv - Microbiology 2020Quote: ... Fungal hyphae were stained with 0.5 mM 4.4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (C1-BODIPY 500/510 C12, Thermo Fisher Scientific) or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysates were centrifuged at 17,800 × g for 5 min at 4°C and supernatants were mixed with NuPAGE LDS Sample Buffer (4×) buffer (Thermo Fisher Scientific) containing 1% (v/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysates were centrifuged at 20,800 × g for 5 min at 4°C and supernatants were mixed with NuPAGE LDS Sample Buffer (4×) buffer (Thermo Fisher Scientific). For SDS-PAGE ...
-
bioRxiv - Biochemistry 2020Quote: ... and 4 μg ml−1 Blasticidin (Thermo Fisher), single cell colonies were collected and subsequently expanded ...
-
bioRxiv - Developmental Biology 2022Quote: ... anti-Ki67 (ThermoFisher, 4-5698-82, 1:100), anti-Actinin (Cell Signaling ...
-
bioRxiv - Bioengineering 2020Quote: ... 4’-6-diamidino-1-phenylindole (DAPI, Life Technologies) was applied at 1 μg/mL for 90 minutes to stain the nuclei and the samples were washed and mounted with AquaPoly/Mount (Polysciences) ...
-
bioRxiv - Cell Biology 2022Quote: ... ERCC 1:4 × 107 dilution (Ambion, cat: 4456740) in 384-well plates prior to library preparation.
-
bioRxiv - Genomics 2021Quote: ... 1 mL of hexane (Fisher Scientific; H303-4) followed by 1 mL of deionized water was added to each sample ...
-
bioRxiv - Bioengineering 2023Quote: ... and 4 μM Ethidum homodimer-1 (Invitrogen™) in PBS for 30 min at 37°C ...
-
bioRxiv - Genomics 2023Quote: ... 4 µL DNase I (ThermoFisher, 1 unit/µL), 4 µL RNase A (ThermoFisher ...
-
bioRxiv - Plant Biology 2022Quote: ... A second round of reverse transcription was initiated with the addition of 4 µL of reverse transcription mix (4:2:1:1 of First Strand Buffer, 0.1 M DTT, RNaseOUT, SuperScript II; ThermoFisher Scientific) to each sample then incubated at 25□ for 10 min ...
-
bioRxiv - Cell Biology 2022Quote: ... the samples were added with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 µg mL-1 in PBS, Invitrogen) and phalloidin conjugated Alexa Fluor dye (Invitrogen ...
-
bioRxiv - Genomics 2022Quote: ... resuspended in 1 mL NSB with 1:20 dilution of 0.25 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) and FACS sorted ...
-
bioRxiv - Biophysics 2023Quote: ... the samples were added with a DNA-binding dye 4’,6-diamidino-2-phenylindole (DAPI, 1 μg mL-1 in PBS, Invitrogen) along with the secondary antibody solution ...
-
bioRxiv - Bioengineering 2023Quote: ... RPMI 1640 containing 2 μM calcein AM and 4 μM EthD-1 along with 1 mg/mL DAPI (ThermoFisher, 62248) was added to the 384-deep well plate (80 μL/well) ...
-
bioRxiv - Neuroscience 2023Quote: ... Media was partially renewed every 3-4 days with neuronal differentiation media 2 (NDM2: 1:1 DMEM/F12:NB (Gibco), glutamax (Gibco) ...
-
Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 1.5 μg of rRNA subtracted total RNA or 10 μg of in vitro-transcribed RNA was added to 4 μl 5× first strand buffer (ThermoFisher Scientific), 1 μl 10μM reverse primer ...
-
Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 1.5 μg of rRNA subtracted total RNA or 10 μg of in vitro-transcribed RNA was added to 4 μl 5× first strand buffer (ThermoFisher Scientific), 1 μl 10μM reverse primer ...
-
bioRxiv - Neuroscience 2020Quote: ... EV lysates corresponding to 5–10 µg of total protein were run into 4-12% Bis-Tris plus gels (Invitrogen, ThermoFisher Scientific). Prior to loading ...
-
bioRxiv - Biochemistry 2021Quote: ... and insoluble samples and were denatured in reducing Laemmli buffer at 100°C for 5-10 min prior to loading onto a 4-20%Tris-Gly SDS-PAGE gel (Life Technologies). Proteins were then transferred to a nitrocellulose membrane using an iBlot2 (Life Technologies) ...
-
bioRxiv - Molecular Biology 2022Quote: IP samples were eluted with 40 µl 1xLDS buffer adjusted to 10 mM DTT (5 min, 90°C) and separated on 4-12% Bis-Tris NuPAGE gels (Thermo Fisher) followed by colloidal coomassie staining (Instant Blue ...
-
bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
-
bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were then incubated overnight with primary antibodies at 4°C in B/P buffer containing either 1:50 rabbit anti-fatty acid synthetase (MA5-14887; Invitrogen, Waltham, MA, USA) or 1:500 rabbit anti-peroxisome proliferator-activated receptor gamma (ab45036 ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatant was mixed in a 4:1 ratio with 4-methylvaleric acid (Thermo Fisher catalogue number AAA1540506) in 6% v/v phosphoric acid and copper sulfate pentahydrate ...
-
bioRxiv - Developmental Biology 2022Quote: ... Selection of and enrichment for cardiomyocytes was initiated on day 9 by culturing cells for 4 days in RPMI 1640 medium without glucose (Gibco) supplemented with 1x CDM3 supplement (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were washed 4 × 5 min with PBS before incubating with secondary antibodies (1:1000 Alexa fluorophores, ThermoFisher) in blocking solution for 1 hr at room temperature ...
-
In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... clone G-A-5) followed by a secondary antibody solution (4 h, RT, AlexaFluor 594, 1:500, Invitrogen), mounted and coverslipped ...
-
bioRxiv - Developmental Biology 2022Quote: ... centrifuged at 500xg for 5 min at 4°C and resuspended in 1% BSA in PBS (AM2616, Invitrogen) containing 200 U/μl RNase inhibitor (3335402001 ...
-
bioRxiv - Cell Biology 2019Quote: ... caspase-9 (10 µM ZLEHD-R110; Molecular Probes), or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence ...
-
bioRxiv - Neuroscience 2021Quote: ... Mg-ATP 4, Na2-phosphocreatine 10, Na3-GTP 0.2 (pH 7.3, 295 mosmol.kg-1) and 20 µM AlexaFluor488 (Invitrogen). Whole-cell patch-clamp recordings from identified interneurons 5IR_DIC ...
-
bioRxiv - Developmental Biology 2022Quote: ... 4 μl 10 μM dNTP mix and 1 μl RNase inhibitor (40 U/μl, Ambion, Cat# AM2682) was added to each tube containing RNAclean XP beads (input group ...
-
bioRxiv - Genetics 2023Quote: ... 1/10 of each eluate was resolved on a 4–12% NuPage Novex gel (Thermo Fisher Scientific) and stained with SilverQuest staining kit (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2019Quote: ... cell suspensions obtained as above were fixed in 4% paraformaldehyde and cytocentrifuged (Cytospin 4 Shandon, Thermo Scientific, 1200 rpm, 10 minutes). Slides of captured cells (n=3 patients for each group ...
-
bioRxiv - Molecular Biology 2024Quote: ... The mixture of SUMO protease cleavage products was dialyzed overnight (4 ºC, 4 L, 20 mM Tris-HCl, 150 mM NaCl, Slide-A-Lyzer→ cassette, 10 kDa, Thermo Scientific). The hexa-histidine tagged SUMO protein was separated from non-tagged authentic Mpro present in the dialysate by IMAC and collecting Mpro in the flow through ...