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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 8 5 HEXYL 2 FURYL OCTANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 90 µL of supernatant was mixed with 410 µL of IP Dilution Buffer and antibodies (Positive antibody: 10 µL; Negative antibody: 2 µL; FoxO3a antibody: 8 µL (Cat. #720128, Invitrogen)) and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultivated in feeder-free medium using Essential 8 (E8) medium with 2% E8 supplement (50x) (Thermo Fisher Scientific), and 1% Penicillin Streptomycin (5000U/ml ...
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bioRxiv - Synthetic Biology 2023Quote: ... the dehydrated DNA was reconstituted in phosphate-buffered saline (PBS, 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, 2 mM KH2PO4, pH 7.4, Invitro-gen, Thermo Fisher Scientific). To remove any large particulate contaminants the solutions of non-functionalised oligonucleotides were syringe-filtered with polyethersulfone filters (0.22 µm pore size ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 and 8 dpf samples were then incubated for 20 and 80 minutes in 10 µg/ml Proteinase K (Thermofisher), respectively ...
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A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... alcohol solution or PBS was mixed with 8×104 PFU of SARS-CoV-2 mNG in 50 uL of infection media (OptiMEM (Gibco) containing 3 μg/mL TPCK-trypsin) ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... were incubated at 37°C for 2 h and analyzed by SDS-PAGE on a 3-8% tris-acete NuPAGE gel (Invitrogen) and stained with Pierce Silver Stain Kit (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... hTCEpi cells were stimulated by KGMTM-2 containing 20% (v/v) sterile PAO1 supernatant for 8 hours before lysis by TRI reagent (Ambion). Total RNAs were purified using Direct-Zol RNA miniprep kit (Zymo Research ...
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bioRxiv - Neuroscience 2024Quote: ... and submerged in cutting buffer (8 mM Na2HPO4.2H2O, 2 mM KH2PO4, pH = 7.4) in the Micron HV650 (Thermo Fisher Scientific). Coronal sections of 60µm were cut using a frequency of 70Hz ...
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bioRxiv - Biophysics 2019Quote: Peptide digests were dried by vacuum centrifugation and dissolved in 20 µl of 5% formic acid and injected at a flow rate of 5 μl/min onto an Acclaim PepMap 100 μm × 2 cm NanoViper 5-μm C18 trap (Thermo Scientific) using mobile phase A containing water and 0.1% formic acid ...
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bioRxiv - Synthetic Biology 2019Quote: ... × 5 mm 5 µm 100 Å and an Acclaim PepMap RSLC 75 µm × 25 cm 2 µm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... or amino acids (MEM amino acids solution; Gibco, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and under a 12-hour day/night photoperiod schedule (Percival Scientific, IA, USA) with 5% dextrose (w/v) and 0.05% para-Aminobenzoic acid (w/v) (Thermo Fisher). Mosquito oocysts were checked at 10-days post-infection ...
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bioRxiv - Neuroscience 2020Quote: ... MEF were grown as monolayers at 37°C with 5% CO2 in DMEM supplemented with 10% serum and MEM non-essential amino acids (Gibco), or with 5% delipidated fetal bovine serum and 35 μM oleic acid ...
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bioRxiv - Biochemistry 2022Quote: ... the gel was rinsed in Milli-Q water for 5 minutes and stained for 20 minutes with 1x SYBR gold nucleic acid stain (Invitrogen) in 1x TBE ...
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bioRxiv - Genetics 2022Quote: ... as well as fibroblasts and SH-SY5Y cells treated for 5 hours with 100 µM oleic acid (OA) following over-night starvation in Opti-MEM (ThermoFisher). OA is a potent inducer of lipid droplet formation (55) ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: Total RNAs of patients were extracted from either FFPE (Formalin-fixed paraffin-embedded) or frozen sections (5 x 20µ) using RecoverAll Total Nucleic Acid Isolation (Ambion) or Trizol (Ambion ...
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bioRxiv - Developmental Biology 2023Quote: ... An internal standard was prepared from the isotope-labeled standards in 3% acetonitrile with 0.1% formic acid with 5 fmol/µL of Peptide Retention Time Calibration (PRTC) mixture (ThermoFisher Scientific) and 10 µg/mL E ...
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bioRxiv - Cell Biology 2020Quote: ... Gel pieces were incubated in Elution buffer (1% formic acid, 2% acetonitrile in LC-MS grade water (Thermo Scientific)) and dried in a SpeedVac ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then run on a Native PAGE gel and visualized with Alcian Blue (1:20 dilution of 1.25% stock solution in 2% acetic acid) followed by Silver Staining (ThermoFisher) following manufacturer protocols and imaged on the Bio-Rad Chemidoc MP Imaging System.
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bioRxiv - Bioengineering 2022Quote: ... glacial acetic acid) for 2 hours at room temperature (Section 2.14) or methanol-free 4% formaldehyde (ThermoFisher, Section 2.16) and then kept in PBS at 4°C before washing in gradations of ethanol up to 100% ...
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... we prepared a 2-fold dilution series of a 1x “stimulation” solution containing 1x essential amino acids (Invitrogen, 11130051), 1x non-essential amino acids (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... were quantified using an avidin and 4’-hydroxyazobenzene-2-carbocylic acid assay according to the manufacturer’s instructions (Fisher Scientific). Briefly ...
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bioRxiv - Physiology 2019Quote: ... Cells were incubated with fura-2AM (2 μM) (Molecular ProbesTM) in SBS containing 0.01% pluronic acid (Thermo Fisher Scientific) for 60 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were prepared for analysis by staining with 2 x SYBR Green I nucleic acid gel stain (Life Technologies) for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... diluted 2 times with 0.1 % formic acid and desalted on strong cation exchange micro-tips (StageTips, Thermo Fisher scientific) as described72 ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were resuspended in 400 ul NET-2 and extracted with an equal volume of acid-phenol:chloroform (Invitrogen AM9722). After centrifugation at 16,000 x g for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... by recording the increase in the absorbance at 405 nm after the reaction of reduced glutathione with 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB) for 2.5 min at 15-s intervals using a Varioskan Flash (Thermo Fisher). GSSG was quantified after derivatization of GSH with 2-vinylpyridine ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Fractions were resuspended in 2% acetonitrile and 0.1% formic acid and separated on an Easy nLC 1200 (ThermoFisher Scientific) and a 22 cm long ...
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bioRxiv - Bioengineering 2023Quote: ... 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB, aka Ellman’s Reagent) and DL-dithiothreitol (DTT) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Bioengineering 2023Quote: PEGαMA hydrogels were made by dissolving the PEGαMA in pH 8.4 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies) at 12.5-15.5 weight percent (wt%) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... while IL-8 production was evaluated using human IL-8 ELISA kit (Thermo Scientific), with assay range of 2-250 pg/mL.
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
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bioRxiv - Cell Biology 2024Quote: ... the conversion of non-fluorescent 2’,7’-bis-(2- carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Invitrogen, Waltham, MA) into a pH sensitive fluorescent indicator by the intracellular esterase was used to measure the pH ...
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bioRxiv - Cancer Biology 2024Quote: ... Organoid proliferation used the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) for 2 hrs using Click-iT EdU Alexa Fluor-555 Imaging Kit (Invitrogen, C10338). H&E and IHC slides were viewed by brightfield-fluorescent microscopy (Olympus BX60) ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 8% FBS (GIBCO) and 20% L929 supernatant and antibiotics (penicillin ...
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bioRxiv - Plant Biology 2022Quote: ... Di-8-ANEPPS (Molecular Probes, Life Technologies ...
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bioRxiv - Immunology 2022Quote: 8-well Labtek chambers (Nunc) were coated with 15 μg/ml of anti-IgM and/or anti-IgG with or without 0.5 μg/ml of mouse histidine-tagged ICAM-1 (Sino Biological ...
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bioRxiv - Genetics 2020Quote: ... pH 8 solution (Ambion, #AM9262), add 1 mL 14.3M β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... Essential 8 Flex (Life Technologies), on growth factor-reduced Matrigel® (Corning)-coated plates and routinely passed with Relesr (Stem Cell Technologies) ...
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bioRxiv - Immunology 2021Quote: ... 8% horse serum (Gibco 16050130), 1% non-essential amino acids (Lonza BE13-114E) ...