Labshake search
Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 7α 12α Dihydroxycholest 4 en 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% PFA for 20 min at RT and then washed (3×) in DPBS with calcium and magnesium (DPBS +/+, Gibco Invitrogen). For structural proteins ...
-
bioRxiv - Cell Biology 2023Quote: ... digitonin-solubilized (4 g/g) mitochondria (10–50 μg) were loaded in NativePAGE Novex 3–12% (vol/vol) gels (Life Technologies). After electrophoresis ...
-
bioRxiv - Cell Biology 2023Quote: ... maintained at 37° C and 5% CO2 and passaged every 3-4 days using trypsin-EDTA 0.05% (Life Technologies, Paisley, UK) to detach the cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... and blotted on two sides for 3 sec at 4 °C and 100% humidity before vitrified in liquid ethane using a Vitrobot Mark IV (Thermo Fisher). The grids were subsequently transferred to a Titan Krios (G3i ...
-
bioRxiv - Biochemistry 2024Quote: The sample was blotted for 3 s with blot force 20 at 100% relative humidity and 4 °C using a Vitrobot Mark IV (Thermo Scientific). Grids were plunge frozen in liquid ethane cooled by liquid nitrogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... these plasmids were transfected into HEK293T cells alongside psPAX2 and pMD2.G at a mass ratio of 1 pMD2.G : 3 psPAX2 : 4 pLKO.1 using Lipofectamine 2000 (Invitrogen 11668019), and media containing the lentivirus was concentrated with Lenti-X Concentrator (Takara Bio 631232 ...
-
bioRxiv - Cell Biology 2020Quote: ... Total RNA was isolated from duplicate or triplicate samples and concentrations were measured using the NanoDrop One/One Microvolume UV-Vis Spectrophotometer (Thermofisher). CDNA was synthesized using the iScript cDNA synthesis kit (Biorad ...
-
bioRxiv - Genetics 2019Quote: ... For deletions MEL745a cells were transfected with one plasmid targeting upstream and one plasmid targeting downstream sequence surrounding the enhancer using Lipofectamine 3000 (Invitrogen) according to manufacturer instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was reverse-transcribed to cDNA using a one-step cDNA Synthesis Kit (Applied Biosystems, USA). Primers used in the study were designed using PrimerBlast tool of NCBI (Table 1) ...
-
bioRxiv - Microbiology 2021Quote: ... one-step quantitative real-time PCR was performed using SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit (Invitrogen) with primers specific for the TRS-L and TRS-B sites for the N gene as well as ACTB as an internal reference ...
-
bioRxiv - Microbiology 2020Quote: ... One-step qRT-PCR was performed using the Invitrogen EXPRESS One-Step Superscript qRT-PCR kit (Thermo Fisher Cat #11781200) and the commercial Taqman probe ACE2 (Hs01085333_m1) ...
-
bioRxiv - Systems Biology 2021Quote: ... The amount and purity of the RNA samples were quantified using a Nanodrop One/OneC Microvolume UV–VIS Spectrophotometer (ND-ONE-W) from ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... One-step RT-PCR was performed using the Super-Script III One-Step RT-PCR System with Platinum Taq (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... one-step quantitative real-time PCR was performed using SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit (Invitrogen) with primers specific for the TRS-L and TRS-B sites for the N gene as well as ACTB as an internal reference ...
-
bioRxiv - Cancer Biology 2022Quote: ... all exosome samples were diluted with one volume of Exosome Resuspension Buffer and one volume of 2X Denaturing Solution (both from Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed using One-Step Real-Time RT-PCR Master Mixes (Thermo Fisher Scientific, Waltham, USA) and the StepOnePlus Real Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: LLC-MK2 cells were seeded in 2 × 96-well plates (one for inhibition and one for cytotoxicity) in complete media (DMEM from Gibco, Cat ...
-
bioRxiv - Microbiology 2023Quote: ... A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen). To amplify the pol region ...
-
bioRxiv - Neuroscience 2023Quote: One million cortical cells and one million striatal cells were suspended in 6 mL D-minimum essential medium (DMEM, GIBCO) with 10% foetal bovine serum (DMEM+) ...
-
bioRxiv - Microbiology 2023Quote: ... A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen). The one-step RT-PCR was performed using the forward primer Pol F1 5’-TACAGTGCAGGGGAAAGAATA-3’ (nt 4809-4829 ...
-
bioRxiv - Microbiology 2024Quote: ... and used to amplify a 204bp amplicon in a one-step RT-PCR reaction (SuperScript IV One-Step, Thermo Fisher) using custom primers (Table 1) ...
-
bioRxiv - Microbiology 2020Quote: ... and NanoDrop One (Thermo Scientific, Waltham, Massachusetts, USA). pH-CaCl2 and pH-H2O of ms soils was measured as described earlier (section 2.2.2 ...
-
bioRxiv - Microbiology 2020Quote: ... coli DH5α and One Shot™ PIR2 (Thermofisher) were required for standard genetic manipulations and plasmid maintenance (Tables S1 and S2).
-
bioRxiv - Cancer Biology 2021Quote: ... in a Step One Thermal Cycler (Applied Biosystems) essentially as indicated (Rico-Leo et al. ...
-
bioRxiv - Microbiology 2021Quote: ... and electroporated into One Shot Top10 cells (Invitrogen). The plasmid containing the cloned chitinase gene was isolated using the Qiaprep spin miniprep kit (Qiagen) ...
-
bioRxiv - Molecular Biology 2020Quote: ... on a Step One Plus (Applied Biosystems, USA) machine ...
-
bioRxiv - Genomics 2020Quote: ... 1μl of RNase One (10U/μl) (Thermo Scientific), and 1μl of Benzonase Nuclease (250U/μl ...
-
bioRxiv - Neuroscience 2019Quote: ... one drop of each ReadyProbes reagent (Life Technologies) was added to cells in 500 μL of media ...
-
The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... one part of Ham’s F12 medium (Life Technologies), 10% fetal bovine serum (Hyclone) ...
-
bioRxiv - Microbiology 2019Quote: ... 1μl of RNase One (10U/μl) (Thermo Scientific), and 1μl of Benzonase Nuclease (≥250U/μl ...
-
bioRxiv - Developmental Biology 2020Quote: ... in a step one thermal cycler (Applied Biosystems) essentially as described (Rey-Barroso et al. ...
-
bioRxiv - Biochemistry 2020Quote: ... A Step One Plus PCR system (Applied Biosystems) was used for incubation at 37 °C as well as for fluorescence measurements over 710 minutes.
-
bioRxiv - Evolutionary Biology 2020Quote: ... A one-step hemi-nested RT-PCR (Invitrogen) was used to detect coronavirus RNA using a set of primers targeting a 440-nt fragment of the RdRp gene and optimized for bat-CoV detection (CoV-FWD3 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1:2000 for one hour and DAPI (Invitrogen) 1:2000 for 15 minutes ...
-
bioRxiv - Biochemistry 2020Quote: One Shot™ Stbl3 (Fisher Scientific, Cat. # C737303)
-
bioRxiv - Biochemistry 2020Quote: One Shot™ TOP10 (Fisher Scientific, Cat. # C404003)
-
bioRxiv - Immunology 2020Quote: ... One µM Sytox blue or Sytox Green (ThermoFisher) was used to measure inner membrane damage ...
-
bioRxiv - Microbiology 2019Quote: ... A spectrophotometer was used (Thermo Scientific NanoDrop One) to measure the concentration and purity of the DNA extracts ...
-
bioRxiv - Microbiology 2020Quote: ... coli One-Shot TOP10 or DH5α strains (Thermofisher). H ...
-
bioRxiv - Cell Biology 2021Quote: ... in One Shot Top10 chemically competent cells (Invitrogen). Proper insertion was confirmed by sequencing ...
-
bioRxiv - Cancer Biology 2021Quote: ... One hundred µL of D-Luciferin (88292, Thermofisher, Waltham ...
-
bioRxiv - Genetics 2022Quote: ... and a NanodropTM One (ThermoFisher, Waltham, MA, USA) to determine the quantity and quality of DNA released with FUSE and control samples ...
-
bioRxiv - Neuroscience 2022Quote: ... 1X Culture One (all from Thermo Fisher Scientific), 40 ng/mL BDNF (PeproTech) ...
-
bioRxiv - Genetics 2020Quote: ... on the Step One Plus (Thermo Fisher Scientific). Reactions were performed in technical duplicates ...
-
bioRxiv - Microbiology 2021Quote: ... and Nanodrop One (Thermo Fisher Scientific, MA, USA). Quality of RNA samples was assessed by Agilent 2100 system (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2020Quote: ... SuperScript IV One-step RT-PCR kit (ThermoFisher) was used for detection of transcripts ...
-
bioRxiv - Immunology 2020Quote: ... supplemented with One Shot dialyzed FBS (Thermo Fisher), 1mM sodium pyruvate ...