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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Spleens from 12-52 week old C57BL/6 mice were mashed through a 40-μm cell strainer treated with ACK lysis buffer (1 mL per spleen, ThermoFisher) for 5 min at 25°C to lyse red blood cells ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Cell Biology 2021Quote: ... They were washed in PBST for 6 × 10 min before being incubated with secondary antibody (1:400 in PBST) (ThermoFisher) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was extracted from 1 confluent well of a 6-well plate using RNA PureLink RNA mini kit (Life Technologies) as per manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... 200 ng of nLYN-mCherry and 500 ng of iLID plasmid were mixed with 2.1 ul (1 to 3 ratio) of Fugene 6 in 125 ul of Opti-MEM medium (Thermofisher Scientific). After 10 minutes incubation at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-FU-D131-#6) were labeled by 15 min incubation with 1 μM CFSE (CellTrace CFSE, ThermoFisher Scientific, Ref: C34554). Cells were then resuspended in PBS supplemented with 30% Percoll ...
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bioRxiv - Microbiology 2021Quote: ... the cells were counterstained in 1 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific GmbH, Bremen, Germany) for 10 min at 46°C ...
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Enteroids were then washed with PBS three times with the addition of 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (1:5000) (Invitrogen) for the final 10 min wash ...
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bioRxiv - Microbiology 2022Quote: ... 3 µg RNA for each sample were mixed with 6 µl of ERCC ExFold RNA spike-in mixes diluted to 1:100 (Invitrogen). Next ...
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bioRxiv - Immunology 2023Quote: ... One day prior to the co-culture 70- and 20-4_1BBζ cells were defrosted and cultured in a density of 1 × 10^6 cells/mL in RPMI containing1X GlutaMAX (Gibco), 1X P/S and 10% FCS supplemented with 10 ng/mL IL7 and IL15 overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples that were subjected to RNase A digestion to detect nuclease-resistant disomes were incubated for 1 h at 25 °C with 15 μl of RNase A (1:1,000 dilution) then treated with 6 μl of SUPERase-In RNase Inhibitor (Thermo Fisher). Sucrose gradients of 10-50% were prepared using a Gradient Master 108 (Biocomp ...
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bioRxiv - Microbiology 2023Quote: ... around 3 to 5 μg total RNA from samples were loaded onto a 6% formaldehyde-containing 1% agarose gel in the fume hood with RNA Millenium Marker (Ambion) as RNA ladder ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed and stained with 1:10,000 DAPI (4=,6-diamidino-2-phenylindole, Thermo Fisher Scientific; catalog #: D3571) for 15 minutes at room temperature before coverslips were applied using PBS + glycerol as mounting medium.
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with a total of 1 µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Plant Biology 2023Quote: ... for 40 cycles (15 seconds at 95°C, 1 minute at 60°C) in a QuantStudio 6 Flex instrument (ThermoFisher). Percentage of input was calculated as 100* e(CtIN – log2(DF ...
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bioRxiv - Cell Biology 2023Quote: 6-well plates were coated with a solid base of 1 mL of agar (0.8% in complete DMEM) (Thermo Fisher). On top of this solid base ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was again purified as above and a ligation reaction between 1:6 ratio of backbone:vector was carried out with T4 DNA ligase (Invitrogen, 15224017). Ligation mixture was incubated at 10 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with 0.1% Triton X-100 in PBS supplemented with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/ml) (Invitrogen, # 62248) for 30 min ...
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bioRxiv - Immunology 2023Quote: Zebrafish larvae (6 day-post-fertilization) were incubated in embryo medium with 1% DMSO and 5 mM EdU (Invitrogen C10340) for 8 hours at 29°C after the 16 hour Vc infection ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
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bioRxiv - Cell Biology 2024Quote: ... both at 1:300 dilution and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen). Confocal microscopy (Olympus FLUOVIEW FV3000 ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Immunology 2019Quote: ... or 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... 10 mM Laurdan (6-Dodecanoyl-2-Dimethylaminonapthalene, Invitrogen) stock solution was prepared in 100% dimethylformamide (DMF ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR (QuantStudio 6, Applied Biosystems, Waltham, MA) was used to measure gene expression levels and normalized to the housekeeping gene hypoxanthine guanine phosphoribosyl transferase (Hprt) ...
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bioRxiv - Cell Biology 2022Quote: ... on a QuantStudio 6 Flex instrument (Applied Biosystems) in a 96 well format ...
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bioRxiv - Neuroscience 2019Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies) staining was added to visualize nuclei.
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bioRxiv - Immunology 2019Quote: ... using a Quant Studio Flex 6 (Applied Biosystems). Samples were run in triplicate wells in a 96-well plate ...
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bioRxiv - Microbiology 2019Quote: ... on a QuantStudio 6 Flex machin (Applied Biosystems). Genome equivalent (GE ...
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bioRxiv - Immunology 2020Quote: ... using a Quant Studio Flex 6 (Applied Biosystems). Samples were run in triplicate wells in a 96-well or 384 well plate ...
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bioRxiv - Microbiology 2019Quote: ... and 3-6 μl Lipofectamin 2000 (Life Technologies). See the Supplemental Information for detailed description.
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bioRxiv - Bioengineering 2022Quote: ... 6-diamidino-2-phenylindole (Thermo Fisher Scientific, D1306) for 45 minutes at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... media was changed to Essential 6 media (ThermoFisher) and was replaced daily for 4 days ...
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bioRxiv - Bioengineering 2021Quote: ... 6 mL FACS Rinse (Thermo Fisher, BD 340346), 6 mL 80% ethanol ...
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bioRxiv - Bioengineering 2020Quote: ... 6-well culture plates (Corning; Thermo Fisher Scientific), and overlaid with glass coverslips to aid in tissue adherence ...
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bioRxiv - Biochemistry 2020Quote: ... 6-Diamidine-2’-phenylindole dihydrochloride (DAPI, Molecular Probes).
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bioRxiv - Biophysics 2021Quote: ... 6 mM L-glutamine (Thermo Fisher Scientific, USA), 0.2 % (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... 4’,6- diamidino-2-phenylindole (DAPI; Invitrogen D1306) was added during the secondary antibody incubation at a concentration of 700 ng/ml ...
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bioRxiv - Plant Biology 2021Quote: ... Gel (Novex TBE 6%, Invitrogen, cat. no. EC6265BOX) selection and purification was performed to enrich libraries containing siRNA fragments ranging from 20 to 30 nt ...
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bioRxiv - Genomics 2020Quote: ... in a QuantStudio™ 6 system (Applied Biosystems). See Supplementary Table 1 for primer sequences used in this study ...
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bioRxiv - Microbiology 2019Quote: ... For TMT 6-plex labeling (Thermo Fisher Scientific), digested peptides were treated according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 6 diamidino-2-phenylindole (DAPI) (ThermoFisher scientific, 10116287) and mounted with Fluoromount-G (Cambridge Bioscience) ...
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bioRxiv - Cancer Biology 2021Quote: ... or QuantStudio-6 Real-Time System (Applied Biosystems). Power SYBR Green Cells-to-Ct kit was used to perform quantitative PCR on 10,000 cells sorted from tumors ...
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bioRxiv - Neuroscience 2022Quote: ... in Essential 6 medium (Thermo Fisher Scientific, A1516401) supplemented with two SMAD pathway inhibitors - dorsomorphin (2.5 μM ...
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bioRxiv - Cell Biology 2022Quote: ... 6-diamidine-2′-phenylindole dihydrochloride (Thermo Fisher Scientific) was added for nuclear counterstaining ...
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bioRxiv - Neuroscience 2022Quote: ... and 6 μl of NFW (Ambion™, AM9937). The PCR reaction was carried out using the following conditions ...
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bioRxiv - Biophysics 2022Quote: ... 6 μL PageRulerPlus Prestained Protein Ladder (ThermoFisher Scientific) was loaded to monitor the progress of the SDS-PAGE and to estimate the approximate size of the protein after staining the gel ...
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bioRxiv - Bioengineering 2022Quote: ... 2-6 mL (Thermo Scientific, catalog no. 88516), and the final protein concentration was measured via A280 absorbance.