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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... the protein samples were separated on 4-20% SDS-PAGE or Bolt™ 8% Bis-Tris gels (Invitrogen) for 1 h and blotted onto PVDF membrane ...
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bioRxiv - Cancer Biology 2019Quote: ... 8-30μg of whole-cell extract was separated on precast 4 to 12% bis tris protein gel (Invitrogen) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions (200 μL) were incubated for 4 h in 8-well Nunc Lab-Tek chambers (Thermo Scientific #155361). Brightfield and Cy3 fluorescence images were captured on a Nikon Ti Eclipse wide-field microscope ...
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bioRxiv - Developmental Biology 2021Quote: ... A second RNA extraction was performed by adding 250uL of Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) (Ambion, Cat#AM9720) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was then extracted by mixing samples with Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) (Thermo Fisher, AM9722), incubating at 65°C ...
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bioRxiv - Systems Biology 2023Quote: ... The samples were resuspended in 500 μL of Acid-Phenol:Chloroform (with IAA, 125:24:1, pH 4.5, Thermo Fisher Scientific), 500 μL of Buffer B (200mM NaCl (DOT Scientific) ...
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bioRxiv - Immunology 2020Quote: ... plates were incubated with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) substrate (ABTS, Thermo Fisher Scientific) for 15 min at RT shielded from light and absorbance was measured at optical density (OD ...
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bioRxiv - Cell Biology 2019Quote: ... Slides were washed 3 × 10 min with PBS and nuclei stained with 1 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) in PBS for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and female mice (aged 3-8 weeks) were transduced with 250,000 TU in serum-free media (Opti-MEM; Gibco).
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bioRxiv - Developmental Biology 2019Quote: ... chilled on ice for 5 min and electrophoresed in a 3 – 8% Tris-Acetate NuPAGE gel (Novex Life Technologies). Transfer of the proteins onto a nitrocellulose membrane was done in transfer buffer (5% methanol ...
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bioRxiv - Immunology 2021Quote: ... and proteins with a molecular weight above 100 kDa were separated on 3-8% Tris/Acetate gels (Life Technologies) in MOPS Buffer (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... Digestion products were resolved in 3-8% tris-acetate SDS-PAGE followed and visualized by Zinc staining (Life Technologies). Images acquisition (Chemi-Doc Camera ...
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bioRxiv - Biochemistry 2020Quote: Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 3-8% Tris-Acetate gels (Invitrogen, Carlsbad, CA), as described previously [27] ...
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bioRxiv - Microbiology 2021Quote: ... A total of 20 μg of whole cell lysate were resolved on a 3-8% Tris-Acetate NuPAGE (Invitrogen) and analyzed by immunoblotting with anti-SPTBN1 ...
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bioRxiv - Immunology 2020Quote: ... following SDS-PAGE in reducing and non-reducing conditions using NuPAGE™ 3-8% Tris-Acetate gels (Life Technologies). Purified proteins were dialyzed overnight against PBS using Slide-A-Lyzer® dialysis cassettes (10 kDa MW cut-off ...
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bioRxiv - Neuroscience 2022Quote: ... 2010) as follows: protein samples were separated in NuPAGE™ 3-8% Tris-Acetate Protein Gels (Thermo Fisher EA03755) in Tris-Acetate-EDTA running buffer (Thermo Fisher BP13354 ...
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bioRxiv - Developmental Biology 2022Quote: ... 20μg of each respective sample was loaded onto a 1mm 3-8% Tris-Acetate Mini Protein gel (NuPAGE) along with 10μl of Spectra Multicolor High Range protein ladder (Thermofisher). The gel was run at 80V for 15 minutes and then increased to 120V until the dye front reached the bottom ...
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bioRxiv - Immunology 2019Quote: ... while proteins above 200 kDa were blotted on a 3-8% Tris-Acetate gels in MOPS Buffer (Life Technologies). Proteins were separated at 200V for approximately one hour and transferred onto either PVDF or nitrocellulose membrane using the TransBlot Turbo Transfer (BioRad ...
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bioRxiv - Genetics 2020Quote: ... Samples (5 μL each) were loaded into wells of a NuPAGE Tris-acetate 3 – 8% polyacrylamide gel (ThermoFisher Scientific) and electrophoresed for 60 min at 15 volts/cm ...
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bioRxiv - Biophysics 2021Quote: ... and incubated (95 °C, 20 min) before SDS-PAGE using 3–8 % Tris-Acetate protein gels (Thermo Fisher Scientific). After transfer onto PVDF membranes (iBlot 2 Dry Blotting System ...
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bioRxiv - Cell Biology 2023Quote: ... 25% of the total immunoprecipitated material (per condition) was loaded onto a 3-8% Tris-Acetate gel (Thermo Fisher), fully resolved gels were transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 95C and subjected to NuPage 3-8% Tris-Acetate gel (#EA0375PK2, Invitrogen). Separated proteins were transferred to cellulose membrane using a semi-dry transfer method and blocked in TBS-T with 5% dry milk containing 2% normal goat serum for 1hr ...
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bioRxiv - Cell Biology 2023Quote: ... boiled at 95°C for 5 min and loaded into a NuPAGE 3-8% Tris-Acetate Gel (Thermo Scientific) along with HiMark™ Pre-stained Protein Standard (Thermo Scientific LC5699) ...
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bioRxiv - Neuroscience 2023Quote: ... The cytosol and membrane fractions were separated on the NuPAGE Novex Tris-acetate 3–8% gel (Thermo Fisher Scientific) or the PAG mini SuperSep Ace Tris-glycine 5–20% gel (FUJIFILM Wako Pure Chemical) ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were run in a 3-8% Tris-acetate gel with 1X NuPAGE MES Running buffer (Life Technologies, Invitrogen). A wet transfer was done overnight at 30 V ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were run in a 3-8% Tris-acetate gel with 1X NuPAGE MES Running buffer (Life Technologies, Invitrogen). A wet transfer was done overnight at 30 V ...
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bioRxiv - Microbiology 2021Quote: ... The vesicles were formed encapsulating the fluorescent membrane-impermeable dye 8-hydroxypyrene-1,3,6-trisulfonic acid from Thermo Fisher (HPTS, 50 μM). The microfluidic platform was operated by two positive pressure-driven pump modules (MFCS-4C ...
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bioRxiv - Systems Biology 2021Quote: ... at 40°C using a linear gradient of 120 minute shallow gradient rising from 8 % (v/v) acetonitrile/0.1 % (v/v) formic acid (Fisher Scientific, UK) to 30 % (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... All data were normalized to the total protein content determined in protein pellets after neutralization with 10 N NaOH and sonication in 2% SDS plus 8 mM EDTA using a bicinchoninic acid assay (ThermoFisher Scientific) and bovine serum albumin as a standard.
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bioRxiv - Microbiology 2020Quote: ... N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher cat. A33952), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... MF002 - plus-10) and the PCR products were fractionated on 8% TBE gel stained with SYBR Gold nucleic acid stain (Invitrogen, S11494). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher, catalog #A33952), following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... The filtered lysate was diluted 1:2 in purification buffer UA (8 M Urea, 50 mM sodium acetate [Acros Organics, Carlsbad, CA], pH 4). The protein was then purified using a cation exchange HiPrep SP HP 16/10 (Cytiva ...
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bioRxiv - Biophysics 2021Quote: ... solution (pH 8) and incubated in 0.1M sodium bicarbonate solution (pH 8) with 2 uM Alexa Fluor™ 647 NHS Ester (Invitrogen, A37573) for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSC were cultured feeder-free on Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix and maintained in Essential 8 Flex Basal Medium supplemented with Essential 8 Flex Supplement (50x) and 0.1% Pen/Strep (all from Thermo Fisher Scientific), at 37°C under normoxic conditions (21% O2 and 5% CO2) ...
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bioRxiv - Developmental Biology 2021Quote: ... were cultured feeder-free on Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix and maintained in Essential 8 Flex Basal Medium supplemented with Essential 8 Flex Supplement (50x) and 0.1% Pen/Strep (all from Thermo Fisher Scientific), at 37°C under normoxic conditions (21% O2 and 5% CO2) ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM EDTA (pH 8) (TE) containing 10 mg/mL RNase A (Thermo Scientific), and incubated at 37 °C for 1 hr ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were permeabilized for 8 minutes at -20ºC in Optima Methanol (ThermoFisher, A456-1)and washed in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... then transferred into a 1:8 dilution of 10 kD fluorescein conjugated dextran (Invitrogen) in Schneider’s Insect Medium (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... followed by immediate neutralization with 1 M Tris-HCl pH 8 (Thermo Fisher Scientific). Following elution from the column ...
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bioRxiv - Cancer Biology 2020Quote: ... and 12 mM 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) (Thermo Fisher Scientific, #M6494). After 4 hours ...
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bioRxiv - Microbiology 2021Quote: ... an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)) assay (M6494, Thermo Fisher Scientific) was conducted in parallel to the infection and kinase inhibitor treatment according to manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Cytotoxicity was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (ThermoFisher: M6494) reduction analysis according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... with or without 0.1uM 6-Hydroxyhexanoic acid (6-HHA, ThermoFisher, B24857.03).
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bioRxiv - Systems Biology 2021Quote: ... 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco), and 1× MEM non-essential amino acids solution (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco), and 1X MEM non-essential amino acids solution (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... 20 nM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Gibco) and 50 µM 2-Mercaptoethanol (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Acros Organics, ThermoFisher), Magnetic nanobeads (Ocean Nanotech ...
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bioRxiv - Biochemistry 2023Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Acros Organics, ThermoFisher), Magnetic nanobeads (Ocean Nanotech ...
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bioRxiv - Bioengineering 2021Quote: ... hESCs were seeded at a density of 25×103 cell/cm2 on Matrigel-coated 6-well plates in Essential 8 medium (ThermoFisher, USA) on day −1 ...