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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 3 7 Diethyl 2 hydrazinoquinoline hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... ViiA 7 RUO software (Applied Biosystems) was used to calculate the amount of RNA relative to wild type animals for the equivalent time points ...
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bioRxiv - Molecular Biology 2023Quote: ... 7 kDa (Thermo Fisher Scientific, 66370) overnight at 4°C against three changes of 1 L refolding buffer (2 M NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 7% FBS (16140071, Gibco) and 10 mg/L ampicillin (171254 ...
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bioRxiv - Neuroscience 2024Quote: ... in a QuantStudio 7 (Thermo Fisher) qPCR machine ...
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bioRxiv - Cancer Biology 2024Quote: ... on QuantStudio 7 pro (Applied Biosystems). For gene expression quantifications the Ct values of the target genes were normalized to GAPDH (human ...
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bioRxiv - Cell Biology 2024Quote: ... or ViiA 7 system (Applied Biosystems). The 2-ΔΔCt method was used to express the normalized abundance of the mitochondrial genes in the WT cells relatively to their normalized abundance in KO cells.
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bioRxiv - Immunology 2024Quote: ... 7% Tryptose phosphate broth (#18050039, ThermoFisher), 1% (v/v ...
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bioRxiv - Immunology 2024Quote: ... 7-AAD (Invitrogen, 00-6993-50) was used to label dead cells ...
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bioRxiv - Microbiology 2024Quote: ... 7 µL molecular grade water (Invitrogen), and 25 µL KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 7 mM magnesium chloride (Invitrogen) was assembled and pre-warmed to 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 7% FBS (Gibco, 16140071) and 10 mg/mL ampicillin (Calbiochem ...
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bioRxiv - Immunology 2024Quote: ... and stained with 7-AAD (ThermoFisher), FITC-conjugated anti-mouse CD11b (1:100 ...
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bioRxiv - Immunology 2024Quote: ... or 7-AAD (Thermo Fisher Scientific) were used according to manufacturer instructions to measure viability ...
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bioRxiv - Developmental Biology 2024Quote: ... 7% foetal bovine serum (FBS; ThermoFisher), 2% B27 (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... on QuantStudio 7 Flex (Applied Biosystems) instrument ...
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bioRxiv - Microbiology 2021Quote: ... cells were collected from popliteal lymph nodes 7 post immunization and surface stained (2×106 cells) with Fixable Blue Dead Cell Stain (Life Technologies), spike-AF647 (1 μg ...
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S-nitrosoglutathione reductase deficiency causes aberrant placental S-nitrosylation and preeclampsiabioRxiv - Physiology 2021Quote: ... intracellular nitric oxide (NO) and peroxynitrite (ONOO‒) were measured by epifluorescence using 2’,7’-dichlorodihydrofluoresceine (H2DCF‒DA, 10 μM; Molecular Probes), 4,5-diaminofluorescein (DAF-2DA ...
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bioRxiv - Plant Biology 2020Quote: ... Detached roots were then incubated in 1X PBS (Phosphate Buffered Saline) buffer with 50 μM H2DCFDA (2’, 7’-dichlorodihydrofluorescein diacetate, Invitrogen, D399) for 30 min shaking at 200 rpm/min at room temperature (Allan and Fluhr ...
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bioRxiv - Cancer Biology 2020Quote: Lungs and LNs were excised and immersed in 4% paraformaldehyde with 10% sucrose and 7% picric acid in PBS for 2 hours and then embedded in Tissue-Tec OCT (Fisher Scientific). 10 micron sections were prepared and slides were stained for B220 ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was resuspended in 1xPBS supplemented with 2 % fetal bovine serum (FBS, Hybclone) and 1 μg/mL 7-AAD (Life Technologies), filtered through a 40 μm strainer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The redox homeostasis of promastigotes was monitored both qualitatively and quantitively by 2-7 dichlorodihydro fluorescein diacetate (DCFDA) (Life Technologies, USA) staining ...
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bioRxiv - Neuroscience 2021Quote: ... Percent of cell death was determined by staining with 7 μM Hoechst 33342 and 2 μM propidium iodide (PI) (Invitrogen, USA). PI were diluted in warm neuronal culture medium and incubated for 5 min and then images were taken by a Zeiss microscope equipped with automated computer assisted software (Axiovision 4.6 ...
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bioRxiv - Cell Biology 2021Quote: ... day 7 and day 19 samples all cells were adherent and collected using 2 ml/well accutase (Thermo Fisher Scientific, A1110501) and a cell scraper ...
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bioRxiv - Microbiology 2021Quote: ... CAS number: 328–42–7), 2-Ketoglutaric acid, disodium salt, dehydrate (>99%, CAS number: 305–72–6) were purchased from Acros Organics, Belgium ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1x PBS twice and incubated for 20 min in dark at 37°C with 20 µM of H2DCFDA (2’,7’- dichlorodihydrofluorescein diacetate; I36007, Thermo Fisher) diluted in serum-free medium ...
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bioRxiv - Immunology 2022Quote: ... reactions were heated to 70 °C for 2 min and 15 μl loaded on 15% polyacrylamide gels containing 7 M urea (Invitrogen, EC8852BOX). Low range ssRNA Ladder (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... fluorescence was observed under a confocal microscope (LSM880NLO, Zeiss, Germany) using 2’,7’-dichlorodihydro fluorescein diacetate (H2DCFDA, 10 InvM, Invitrogen, USA) as described by Yu et al ...
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bioRxiv - Plant Biology 2024Quote: ... Ten-μg protein of each sample were separated by electrophoresis in 15% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Roth) for 7 min at 20V using the iBlot 2 Dry Blotting System (ThermoFisher Scientific), before blocking in TBS-T with 5% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... 7 μl per injection was loaded onto a 2 mm by 0.3 mm Acclaim PepMap C18 trap column (Thermo Fisher Scientific) in 0.1% TFA at 15 μl/min before the trap being switched to elute at 0.25 μl/min through a 50 cm by 75 μm EASY-Spray C18 column ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Pathology 2023Quote: ... The mRNAs were quantified using individual TaqMan assays described in Supplemental Table 2 on an ABI QuantStudio 7 Real-Time PCR machine (Applied Biosystems) using TaqMan Fast Advanced Master Mix (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: Gene expression of targets listed in supplemental table 2 were analysed by SYBR green-based qPCR analysis using Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Thermo Scientific), following the standard PCR cycling sequence using GAPDH as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... plated at a density of 7 x 106 cells in a 175 cm2 tissue culture flask in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... at 1,000g for 2 min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Library concentration was normalized to 2 nM and concentrations validated by qPCR on a ViiA-7 real-time thermocycler (Applied Biosystems), using qPCR primers recommended in Illumina’s qPCR protocol and Illumina’s PhiX control library used as a standard ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated with fresh DMEM containing DDAO galactoside (9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-Galactopyranoside) (Molecular Probes™) for 2 hours ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... coli MG1655 or BW25113 carrying pBAD18-Kn derived plasmids were diluted 1 in 100 into LB media containing 10 µM OxyBURSTTM Green (2’,7’-dichlorodihydrofluorescein diacetate, succinimidyl ester; Invitrogen #D2935) and incubated at 37°C for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... Some slices (used for experiments in Fig. 2 and 3) were cultured in media that included 2 % B-27 Supplement (Thermo Fisher Scientific). Slices were maintained at 37°C and 5 % CO2 and used for experiments at DIV10-15.
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bioRxiv - Neuroscience 2024Quote: ... followed by culturing with the neural induction medium supplemented with 20 ng/ml human FGF-2).61 The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 Pierce™ protease inhibitor mini tablet/2 L; Thermo Scientific). The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 2-3 hours at room temperature with 2 µg/ml donkey anti-goat Alexa Fluor 488 (Cat# A21206, Thermo Fisher Scientific), 2 µg/ml donkey anti-rabbit Alexa Fluor 568 (Cat# A10037 ...
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bioRxiv - Cancer Biology 2021Quote: ... using a 10 min loading at 3 µL/min flow rate to a trap column (Acclaim™ PepMap™ 100, 2 cm × 75 µm, 3 µm, 100 Å - ThermoFisher Scientific). The separation was performed on an EASY-Spray™ C18 analytical column (25 cm × 75 µm ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA expression was analyzed based on 2-3 biological replicates each with 3 technical repeats using QuantStudio™ Design & Analysis Software (Thermo Fisher Scientific Inc.). The 18S-rRNA housekeeping gene was used as an internal reference gene ...
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bioRxiv - Cancer Biology 2020Quote: ... and 12 mM 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) (Thermo Fisher Scientific, #M6494). After 4 hours ...
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bioRxiv - Biochemistry 2020Quote: ... and SARS-CoV-2 [3]) was performed with Proteome Discoverer version 2.4 (Thermo Fisher Scientific). The search was restricted to human and SARS-COV-2 proteins ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μg of RNA was digested with Turbo DNase (2 U/μl, # AM1907, ThermoFisher Scientific). 500 ng of DNase-digested RNA was tagged with G/I tails in a 10 μl reaction ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated for 3 days in presence of 100 U/mL IL-2 (Gibco) and 1 µg/mL PHA (Remel).