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Citations for Thermo Fisher :
6901 - 6950 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Antibody proteins were purified from the culture supernatant after 12-14 days using Protein A bead columns (Thermo Fisher).
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bioRxiv - Cell Biology 2021Quote: ... Total protein concentrations were determined using the Pierce™ Coomassie (Bradford) Protein Assay Kit according to manufacturer’s instructions (ThermoFisher). Equal concentrations of cell lysates were prepared in Laemmli buffer (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts (30 μg) of protein measured by the BCA protein assay kit (23225, ThermoFisher Scientific, Waltham, MA, USA) were loaded into a precast NuPAGE 4-12% gradient Bis-Tris gel ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were clarified by centrifugation and protein levels were quantified using a BCA protein assay kit (Thermo Fisher Scientific). Proteins (50 μg ...
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bioRxiv - Neuroscience 2021Quote: ... An equal amount (500µg) of each protein lysate was precleared for 1 hour with magnetic Protein G Dynabeads (Invitrogen) and immunopurified with 40µl washed anti-V5 agarose beads (Sigma ...
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bioRxiv - Plant Biology 2020Quote: The concentration of the mTurquoise2 protein was determined using a Pierce™ 660nm Protein Assay Kit (#22662, Thermo Scientific) and used as reference to build a mTurquoise2 standard curve (linear regression ...
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bioRxiv - Plant Biology 2020Quote: ... Total soluble protein concentration was estimated using a Pierce™ 660nm Protein Assay Kit as above (#22662, Thermo Scientific).
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bioRxiv - Biochemistry 2021Quote: ... Protein concentrations were determined by the Pierce™ BCA Protein Assay Kit according to manufacturer’s instructions (Thermo Scientific, 23227). Samples were analyzed on a Quadrupole-Time of Flight (Q-TOF ...
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bioRxiv - Biochemistry 2021Quote: ... which was calibrated using an unstained native protein ladder (NativeMark™ Unstained Protein Standard A, Thermo Fisher Scientific Inc.). Measurements were performed on the reconstituted complex I at a concentration of 0.015 mg ml-1 using AcquireMP 2.2.0 software and were analyzed using the DiscoverMP 2.2.0 package.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were sonicated and the total protein amount was determined with a BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... A 1:5 dilution of this protein solution was quantified using a Pierce BCA protein assay kit (Thermo scientific). For analysis of the relative composition of amino acids ...
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bioRxiv - Microbiology 2021Quote: ... per reaction and 50 µL of magnetic protein G-coated Dynabeads according to the supplier’s instructions (Dynabeads Protein G for Immunoprecipitation, 10003D, ThermoFisher). Proteins were eluted in the denaturing mode and run in a 4-20% Mini-Protean TGX stain-free precast protein gel (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... The protein levels in the supernatants were determined using the BCA Protein Assay Kit (Pierce/Thermo Scientific, Rockford, IL). To detect BDNF ...
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bioRxiv - Neuroscience 2020Quote: ... The total protein concentration was measured with bicinchoninic acid protein assay (Pierce™ Micro BCA Kit, ThermoFisher Scientific, USA). Human brain-derived Aβo was characterized by Western blot analysis and atomic force microscopy (details in Additional file 1 ...
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bioRxiv - Microbiology 2021Quote: ... according to manufacturer’s specifications and protein concentration was determined using a bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific). 10 µg of nuclear extract was assayed and DNA binding activities of transcription factors (ATF-2 ...
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bioRxiv - Microbiology 2021Quote: ... Protein digestion was performed by incubating in trypsin (1 µg trypsin/50 µg protein; Thermo Fisher Scientific, Waltham, MA) for 3 h ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was saved and protein was quantified using a Pierce BCA protein assay kit (ThermoFisher, catalog number 23225). 100 ug of protein was used per sample ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was saved and protein was quantified using a Pierce BCA protein assay kit (ThermoFisher, catalog number 23225). 30 ug of protein was used per sample ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration of the mucus released during the irritation test was analysed using NanoOrange protein kit (Invitrogen™) with infinite M200 pro microplate reader with the excitation wavelength of 485 nm and an emission wavelength of 590 nm ...
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bioRxiv - Microbiology 2020Quote: ... The total protein content in the infected cells was determined using a BCA protein assay kit (Thermo Scientific, USA) with bovine serum albumin as standard ...
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bioRxiv - Biochemistry 2020Quote: ... and PrpE were purified as decribed.39,57 Protein concentrations were determined with the BCA Protein Assay Kit (Thermo Scientific). Samples were stored as aliquots at −80 °C until further use.
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bioRxiv - Cell Biology 2021Quote: ... The supernant was collected and the protein concentration determined with the Micro BCATM Protein Assay Kit (Thermo Fisher Scientific). 80 µg protein were loaded into a 10 % SDS gel and stained with anti-Tau (clone Tau46 ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated Golgi proteins were extracted and the protein mass was determined using the BCA method (23225, Thermo Scientific, USA).
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected and total protein concentration was determined using Micro BCA™ protein assay kit (Thermo Scientific). Protein samples were then reduced with 10 mM DTT for 1 h at 60 °C and subsequently alkylated using a 20 mM IAA in dark at room temperature for 30 min ...
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bioRxiv - Cell Biology 2022Quote: The lysates were analyzed by 4-16% Native PAGE using precast Bis-Tris Mini Protein Protein Gels (Invitrogen BN1003BOX) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The concentration of protein in each sample was determined using the Pierce BCA Protein Assay (Thermo Fisher Scientific, 23225) before treatment with 100 mM dithiothreitol at room temperature for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... which was calibrated using an unstained native protein ladder (NativeMark™ Unstained Protein Standard A, Thermo Fisher Scientific Inc.). Measurements were performed at concentrations of 0.1-0.2 mg/ml using AcquireMP 2.2.0 software and were analyzed using the DiscoverMP 2.2.0 package (Extended Data Fig ...
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bioRxiv - Cell Biology 2022Quote: ... The induced proteins were isolated from bacterial pellets lysed in B-PER Bacterial Protein Extraction Reagent (Thermo Fisher Scientific) containing cOmplete ULTRA Protease Inhibitor Tablets (Sigma Aldrich ...
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bioRxiv - Genetics 2022Quote: ... Subcellular protein fractionations were performed using the Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific, Catalog no: 78840) according to the product manual ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration of the lysates was quantified using a BCA Protein Assay Kit (Thermo Scientific Pierce Biotechnology, USA) and Benchmark Plus Microplate Spectrophotometer (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein concentration was measured with a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Waltham, MA) to normalize the insulin level ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant of lysates was assessed for protein content using the Coomassie Protein Assay Reagent (23200, Thermo Fisher Scientific) and spin filtered through Amicon Ultra-0.5 centrifugal filter units (10 kDa MWCO ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected and protein concentration was determined using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Scientific). Equal concentrations of protein extract were loaded into 4%–12% NuPAGE BisTris gels (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Protein quantification of the collected exosomes was performed using BCA method (Pierce™ BCA Protein Assay kit, ThermoFisher Scientific) and equal amount of exosomes were collected for downstream applications ...
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bioRxiv - Cell Biology 2022Quote: ... The MV protein fractions were detected on the protein gel using Simple-Blue™ Safe Stain (ThermoFisher Scientific, UK). The sandwich method was then used to transfer the proteins from the gel to a methanol-activated polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was transferred to a new tube and protein concentrations were determined using Pierce BCA protein assay (ThermoFisher, 23225). Samples were stored at -80 °C until further use ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatant was kept and protein concentration was measured using the BCA method (Pierce BCA Protein Assay, Thermo Fisher, #23225). Samples were incubated in 2x Laemmli Buffer (120mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... The total protein concentration in each cell lysate was measured using Pierce BCA Protein Assay Kit (Thermofisher, Waltham, MA). The cell lysates containing approximately 20 µg of protein were resolved on Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Protein content of whole cell lysate was measured with the BCA protein assay (Pierce™, Thermo Scientific, Landsmeer, Netherlands) to quantify the protein content of the samples in order to equalize for input material for SDS page ...
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bioRxiv - Neuroscience 2019Quote: ... 10 μl/mg) and protein content was measured by the Pierce™ Bicinchoninic Acid protein assay (BCA; Thermofisher Scientific). 5 μl (4 μg protein/μl ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Pathology 2021Quote: ... The protein concentrations of homogenized tissues were determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, Cat# 23225). The homogenized tissues were then incubated with CM-H2DCFDA probe at 37 °C for 20 min with protection from light ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The protein concentration of cleared samples was determined with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, #23225) with measurements conducted on a GloMax-Multi Plate Reader (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was recovered by centrifuge and quantitated by Pierce™ BCA Protein Assay Kit (23227, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Protein lysates were subjected to SDS-PAGE and Western blot.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein concentration was measured using the Pierce™ BCA® Protein Assay Kits (Thermo Fisher Scientific Inc., cat.no. 23227). CD63 antibody (1:500 Thermo Fisher Scientific Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were normalized to equal protein concentrations (0.333 mg/ml) using Pierce BCA protein assay (Thermo Fisher Scientific, USA).
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bioRxiv - Microbiology 2021Quote: ... The supernatant was collected and total protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Biochemistry 2021Quote: ... The total protein concentration of the supernatants was determined by the Pierce bicinchoninic acid (BCA) protein assay (Thermo Scientific). For the pulldown experiments ...