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Citations for Thermo Fisher :
6851 - 6900 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was collected and the protein concentration was measured using a Coomassie Plus Protein Assay (Thermo Scientific).
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bioRxiv - Plant Biology 2021Quote: ... “Protein extracted” with T7 tag antibodies were incubated with protein A containing magnetic beads (Dyna beads, Thermo fisher) for 2 hours at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration of the remaining soluble fraction was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher) and samples with equal total protein quantity were prepared with Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... The protein concentration of the samples was determined using a Pierce BCA Protein Assay Kit (Thermo Scientific, 23225). 40 µg of protein was separated by NuPAGE 4-12% Bis-Tris Protein Gels (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... The concentrations of TIN2 proteins were determined by the BCATM protein assay with BSA as the standard (ThermoFisher). The identities of purified HA-TIN2S and HA-TIN2L proteins were confirmed by the Western Blot analysis using the HA antibody (GenScript A00168) ...
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bioRxiv - Cell Biology 2021Quote: Soluble proteins (10 to 50 μg) were resolved on NuPAGE 4-12% Bis-Tris Protein Gels (Life Technologies) or on SDS-PAGE gels (12 or 15% ...
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bioRxiv - Cell Biology 2020Quote: Eluted proteins were resolved on a 10-well NuPAGE 4-12% Bis-Tris protein gel (Thermo Fisher Scientific), stained with Coomassie (Imperial Protein Stain ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentration was determined using the Micro BCA™ protein assay kit according to manufacturer’s instructions (Thermo Scientific). Each sample was split in two aliquots of 100 μg protein and one additional aliquot of 50 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Beads were prepared by incubating them in 0.5% BSA in PBS and antibodies overnight (100µL of Dynabeads Protein A or Protein G (Invitrogen) plus 20µL of antibody) ...
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bioRxiv - Cell Biology 2022Quote: ... An aliquot of 6 μL was collected for protein quantification using the BCA Protein Assay (ThermoFisher Scientific 23227) and the remaining clarified lysates were kept at −20°C until SDS-PAGE analysis.
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bioRxiv - Cell Biology 2022Quote: ... and protein determination was performed using the Bradford colorimetric assay with BSA as the standard protein (Thermo Scientific). Absorbance was measured at a wavelength of 595nm using a hybrid microplate reader (Biotek).
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration of the resultant lysate was measured using a BCA protein assay kit (Thermo Fisher Scientific, #23227). 80 µg of whole cell lysate was electrophoresed in a 12 % NuPAGE gel and blotted to PVDF membrane overnight at 24 V using a wet transfer blotting module (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... protein concentration of the lysates was measured using the BCA Protein Assay Kit (Thermo Fisher Scientific, Cat#23227) and the samples diluted to 1mg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration in total cell lysates was determined by a BCA protein assay kit (Thermo Fisher Scientific). Equal amounts of protein samples were loaded on 10% glycine-SDS-PAGE gel ...
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bioRxiv - Biochemistry 2022Quote: ... Two milligrams of total protein were incubated overnight at 4°C with Pierce Protein G magnetic beads (ThermoFisher), followed by successive washes ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein were loaded into Nu-Page Novex 4-12% Bis-Tris protein gels (Invitrogen, NP0322) and electrophoresis run in MOPS buffer (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Purified protein was analyzed by gel electrophoresis using NuPAGE Bis-Tris protein gels (Thermo Fisher Scientific, Cat: NP0321PK2). DTT (5mM ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear extracts were pre-cleared with 50 μL protein G conjugated superparamagnetic beads (Dynabeads Protein G, ThermoFisher Scientific) for 30 min at 40C ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentration was then quantified using Peirce™ BCA Protein assay kit (23225, Thermo Fisher Scientific, MA, USA). Processing the samples for proteomics was then continued using a 10 kDa filter aided sample preparation protocol previously published with minor modifications.[30] The proteins were digested using Lys-C enzyme (P8109S ...
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bioRxiv - Biochemistry 2022Quote: Protein samples were separated via SDS-PAGE and either stained with PageBlue Protein Staining Solution (Thermo Fisher Scientific) or used for western blot or ConA lectin blot analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration in the lysate was measured using a BCA protein assay kit (Thermo Scientific, Waltham, MA, USA). Tissue protein diluted in array buffer was incubated with the ready-to-use pre-coated array membranes overnight at 4°C on a rocking platform shaker ...
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bioRxiv - Cancer Biology 2022Quote: ... We next quantified the protein concentration of samples using Pierce BCA protein assay kit (Thermo fisher scientific; 23225). Samples were boiled in 4X NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Quantification of total protein in the lysates was done using Pierce BCA Protein Assay Kit (#23227, Thermo Scientific) following the manufacturer&s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentration from the supernatant was measured using a Pierce BCA Protein Assay Kit (Cat# 23225, ThermoFisher Scientific). Lysates were diluted to 1 μg/μl in an extraction buffer of 1 X NuPAGE LDS Sample Buffer (Cat# NP0007 ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration of the lysate was measured using a PierceTM BCA Protein Assay Kit (23225, ThermoFisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... Proteins in the clear supernatant (protein extract) were cross-linked with 5 mM DSP (Thermo Fisher Scientific Pierce) for 2 h with agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) with the following modifications ...
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bioRxiv - Neuroscience 2020Quote: ... The protein concentration in clarified lysates were measured using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and protein concentration in the supernatant was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). Laemmli buffer was added to the lysate to 1× concentration (2% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration in the cleared supernatant was determined using the Pierce® BCA Protein Assay Kit (Thermo Scientific) following the manufacturer’s instructions and protein samples were loaded on gradient 4-20% Tris-MOPS-SDS gels (GenScript) ...
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bioRxiv - Microbiology 2021Quote: ... protein concentrations were measured using the Pierce bicinchoninic acid protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the ‘Enhanced Test-tube’ protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein ladder SeeBlue™ Plus 2 Pre-stained Protein Standard (Invitrogen™ through Thermo Fisher Scientific, Loughborough, UK) was used ...
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bioRxiv - Cancer Biology 2020Quote: Samples were collected in RIPA buffer and protein concentrations were calculated using Coomassie Plus protein assay (Thermo Scientific) and a standard BSA curve ...
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bioRxiv - Cancer Biology 2020Quote: ... 13μg of each protein sample was loaded onto NuPAGE 4-12% Bis-Tris Protein gels (Thermo Fisher Scientific), and samples were run in NuPAGE MOPS SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Values were normalized to protein content determined as using a Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were clarified by centrifugation and total protein concentration was determined by BCA assay (PierceTM, Thermo Fisher). Fifty μg of protein samples for brain lysates and 20 μg of protein samples were resolved on polyacrylamide gels (see Western Immunolotting for details).
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bioRxiv - Immunology 2020Quote: ZAP70-tSH2 GFP protein was introduced into ILA Jurkat cells by directly electroporating the purified protein (NEON; Invitrogen). Full-length ZAP70-Halotag was transduced into Jurkat cells using a lentiviral system and were labelled with Halotag ligand-Alexa647 made in house by reacting Halotag Amine ligand (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration in the lysates was determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and the cell lysates were stored at −20°C until use ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatant was extracted following centrifugation and protein concentration was assessed using Pierce BCA Protein Assay Kit (Life Technologies). Samples with equal protein (20ug ...
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bioRxiv - Neuroscience 2022Quote: ... and total proteins were isolated using 300 µl of cold mammalian protein extracting reagent (Thermo Fisher Scientific, 78501) containing protease inhibitor cocktail (cOmplete ...
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bioRxiv - Developmental Biology 2022Quote: ... The protein content in lysates was determined using Pierce™ BCA Protein Assay Kit (23225, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Protein homogenates from frozen liver tissue were isolated using T-PER protein extraction reagent (ThermoFisher Scientific, Waltham, MA) containing protease inhibitor cocktails and Halt phosphatase inhibitors (EMD Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Protein concentration was measured using Pierce™ 660 nm Protein Assay Kit (Thermo Fisher Scientific Cat. Nr. 22662) and 400 μg of each cell type-derived protein taken for subsequent MS sample preparation.
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bioRxiv - Microbiology 2022Quote: The concentration of protein from EVs was quantified with a BCA Protein Assay Kit (23225, Thermo-Fisher Scientific). EV samples were aliquoted into 25 µg ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of 6xHis-tagged proteins and Strep-tagged proteins was tested with 6xHIS TagAntibody HIS.H8 (Invitrogen Co.) and StrepMAB-Classic ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration of cell lysates were determined using the Protein Assay Reagent (#23227, Thermo Fisher Scientific, Waltham, MA). Cell lysis buffer was combined in 4× SDS sample buffer and 2-mercaptoethanol and incubated at 95 °C for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... 400 μg of total protein from each sample was immunoprecipitated by incubation with protein-G Dynabeads® (Invitrogen) coupled to a mouse-anti-human VEGF-A antibody (VG-1 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins contained in the eluted fraction were separated using NuPAGE™ 4-12% Bis-Tris protein gels (ThermoFisher). Protein were transferred on nitrocellulose membrane (Amersham™ Protran® ...
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bioRxiv - Neuroscience 2019Quote: ... The protein concentration of the collected supernatant was quantified with Quant-iT™ Protein Assay Kit (#Q33212 Invitrogen), following supplier protocol.
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bioRxiv - Synthetic Biology 2019Quote: Total yeast proteins were extracted using Y-PER™ Yeast Protein Extraction Reagents (Thermo Scientific, Cat no: 78991) following manufacturer’s protocol ...