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Citations for Thermo Fisher :
6851 - 6900 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: 0.5-1 ug of RNA was reverse transcribed to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Invitrogen). Following this ...
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bioRxiv - Pathology 2024Quote: ... and cDNA was synthesized from 1 µg mRNA using a high-capacity cDNA reverse transcription kit (Applied Biosystems, #4368813). mRNA expression levels were measured with a 7500 Real-time PCR system (Applied Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... We generated cDNA from 1 µg of RNA using the SuperScript IV First Stand Synthesis kit (Thermo Fisher Scientific) and analyzed by RT-qPCR using Quant Studio 12K Flex Real Time PCR system version 1.1.2 (Applied Biosystems by Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... 1 μg of purified total RNA was reverse transcribed using the high-capacity cDNA reverse transcription kit (Applied Biosystems). For specific miRNA quantification ...
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bioRxiv - Microbiology 2023Quote: ... Real-time RT-PCR was performed using the Power SYBR Green RNA-to-CT 1-Step Kit (ThermoFisher Scientific) on the AriaMx Real-Time PCR system (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... Dead cells were excluded using the Live/Dead Violet Dead cell stain kit (dilution: 1:750; Thermo Fisher Scientific). Cells were washed with 2% FBS and phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2023Quote: ... per sample (37 °C, 1 hr) and cleaned up using the ChargeSwitch PCR Clean-Up Kit (Thermo Scientific CS12000). Successful immobilization of the BRD9 protein was checked by resuspending the beads in 133.3 μL of NuPAGE LDS Sample Buffer (1× ...
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bioRxiv - Biochemistry 2023Quote: ... per sample (37 °C, 1 hr) and cleaned up using the ChargeSwitch PCR Clean-Up Kit (Thermo Scientific CS12000) (Supplementary Fig ...
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bioRxiv - Biophysics 2023Quote: ... Standard calcium solutions were prepared in triplicate using the Calcium Calibration Buffer Kit #1 (Molecular Probes Cat. No. C3008MP), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The concentration of cDNA was measured by Qubit™ 1× dsDNA High Sensitivity (HS) Assay Kit (Invitrogen, Cat# Q33230). Tagmentation and NGS library was carried out by using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme ...
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bioRxiv - Neuroscience 2023Quote: ... Relative gene expression was determined using the TaqMan® RNA-to-CT™ 1-Step Kit (Thermo Fisher Scientific). Reactions were developed in a LightCycler® 96 (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... 801–1(10 KU)] kit and the qPCR using the SYBR™ Fast Green Master mix (Thermo Fisher, 4385612).
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bioRxiv - Physiology 2023Quote: ... Total RNA (1 µg in 20µl) was reverse transcribed using reverse transcription (RT) kits (Applied Biosystems; Foster City, CA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Real-time RT-PCR was performed with the Power SYBR Green RNA-to-CT 1-Step Kit (Applied Biosystems) using a AriaMx Real-Time PCR system (Agilent).
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bioRxiv - Immunology 2023Quote: ... Viable cells were identified using a LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, 1:200) incubated with conjugated antibodies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 1 µg of RNA was used for cDNA synthesis using High-Capacity RNA-to-cDNA™ Kit (Thermo Fisher) that utilizes both random octamers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubated with Alexa Fluor Tyramide 488 (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Spinal cord single cell suspensions were stained with LIVE/DEAD Fixable Stain Kits (1:1000, L10119 or L34959, Invitrogen) to exclude dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... the single cell suspensions were stained with LIVE/DEAD Fixable Yellow Dead Cell Stain kit (1:1000, L34959, Invitrogen), and surface antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (0.5-1 μg) was reverse-transcribed using a High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific). Real-time PCR was performed using 100 ng of cDNA and ABI StepOnePlus (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5-1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). SYBR Green PCR master mix (Kapa Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of cDNA was synthesized using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Waltham, MA, USA). PCR experiments were performed using 20 ng of cDNA per reaction ...
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bioRxiv - Neuroscience 2024Quote: ... Both fractions were then used to measure the amyloid beta 1-42 (Aβ1-42) using a specific ELISA kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). The mixture was incubated at 25°C for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in PBS and incubated with 1:100 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (Life Technologies), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (1 µg) was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher #4368814). The mRNA levels were detected using amfisure qGreen Q-PCR Master Mix (GenDEPOT #Q5602) ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was syntethized from 1 µg of RNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). For the real-time RT-PCR analysis ...
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bioRxiv - Immunology 2023Quote: ... YT and KHYG-1 cells was performed with the Pierce Cell Surface Protein Isolation kit (Thermo Scientific, cat#89881) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of total DNase I-treated RNA was reverse transcribed using the Superscript first-strand synthesis kit (Invitrogen). The cDNA was diluted in DNase-RNase free water ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of total RNA was transcribed into cDNA by using a SuperScript™ III Reverse Transcriptase kit (Invitrogen) and random hexamer primers ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was synthesized from 1 µg of RNA by following the protocol of cDNA conversion kit (4368814, Applied Biosystems). Real time PCR was performed using SYBR™ Green Master Mix (A25742 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg RNA from HUVECs was subjected to reverse transcription with the High-Capacity RNA-cDNA kit (Applied Biosystems). RT-qPCR was performed from the resulting cDNA in a CFX 384 thermocycler (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... PCR amplicons were cleaned up using QIAGEN’s QIAquick PCR Purification Kit (QIAquick) and separated by electrophoresis on 1% E-Gel EX Agarose Gels (Invitrogen). For sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... JC-1 assay kit (Cat. No. M34152) and Dulbecco’s modified eagle medium (DMEM) (Cat. No. 11965084) were purchased from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: 1 μg of RNA was utilized for reverse transcription using a high capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative RT-PCR was performed using a Bio-Rad CFX96 ThermoCycler (Bio-Rad Laboratories ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 μg total RNA was reverse transcribed with RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, USA) using an oligo (dT ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples (1 μg) were reversely transcribed (High-Capacity cDNA Reverse Transcription Kit; Thermo Fisher Scientific, Waltham, MA, USA) and real-time PCR analysis was performed using 1μl of cDNA in a reaction of 10 μl ...
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bioRxiv - Immunology 2024Quote: ... and viral GP gene amplified by RT-PCR (Verso 1-Step RT-PCR kit; Thermo Fisher Scientific AB-1455), cloned and sequenced as previously described 48.
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bioRxiv - Biophysics 2021Quote: ... The recombinant constructs of heavy and light chain were transfected at 1:1 ratio into Expi293F™ cells using the ExpiFectamine™ 293 Transfection Kit (Gibco™, Thermo Fisher Scientific, Waltham, MA, USA). 4-5 days after transfection the antibodies were purified from the supernatant by protein A affinity resin (ProSep®-vA ultra ...
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bioRxiv - Cell Biology 2023Quote: ... following kit instructions (BCA Protein Assay kit, ThermoFisher #23225) and 20µg loaded into each well of a NuPAGE 4-12% Bis-Tris Gel (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... All luciferase assays were conducted in LumiNunc 96-well white plates (Thermo Scientific, #136101). NanoLuc luciferase was normalized by constitutively expressed firefly luciferase.
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bioRxiv - Biophysics 2019Quote: ... Reactions were performed in triplicate in a 384 well optical plate (Thermo Scientific Nunc). The plate was detected by a Varioskan® Flash microplate reader (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were run on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher), and data were analyzed using the comparative CT method to generate expression fold-change values ...
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bioRxiv - Cell Biology 2020Quote: ... The culture plate was then put into a microplate reader (Thermo Scientific, Multiskan GO) for inspection of the absorbance value at 450 nm and this optical density (OD ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transiently transfected in 6-well plates with Lipofectamine 2000 (Life Technologies), as per the manufacturer’s instructions ...
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SIRT1 ameliorates premature senescence-induced defenestration in hepatic sinusoidal endothelial cellbioRxiv - Cell Biology 2020Quote: ... Primary rat HSECs were cultured in plates with medium comprising 80% MCDB131 (Gibco, 10372019) and 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... 10,000 TZM-bl cells per well were seeded in 96-well plates (Nunc-Greiner). The following day cells were infected with an equal volume of culture supernatant for 4 hr ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were seeded in transparent tissue-culture 96-well plates (Sarstedt, Thermo Fisher Scientific) and allowed to adhere overnight ...
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bioRxiv - Immunology 2021Quote: Macrophages generated as above were harvested from plates using Trypsin-EDTA (Thermo fisher scientific). The indicated target cells were labeled with Celltrace violet (Thermo fisher scientific ...
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bioRxiv - Immunology 2021Quote: ... All qPCRs were performed in MicroAmp Fast Optical 96 WellReaction Plates (Thermo Fisher Scientific) using AppliedBiosystems 7500 Fast Real-Time PCR System with 7500Software V2.0.6 (Thermo Fisher Scientific) ...