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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... For membranes with a mixture of CellBrite Cytoplasmic membrane dye (Biotum) and BODIPY FL C5-Ceramide (Invitrogen) at 400- and 1000-fold dilution ...
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bioRxiv - Neuroscience 2020Quote: ... slices were placed on polycarbonate membranes for 24-well plates (two slices per membrane; Thermo Scientific #140620). Cultures were allowed to mature for at least 18 days before experimental assessment ...
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bioRxiv - Developmental Biology 2021Quote: ... We stained the plasma membrane using CellMask Deep Red Plasma Membrane Stain (Cat. C10046, Thermo Fisher Scientific) according to the manufacturer’s instructions and fixed the cells with 4% of paraformaldehyde ...
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bioRxiv - Biochemistry 2022Quote: ... and thereafter blotted onto PVDF membranes (Immobilon P membranes, 0.45 μM) according to the manufactures manual (Invitrogen). The membrane blots were blocked with phosphate-buffered saline containing 0.2% Tween-20 (v/v ...
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bioRxiv - Biophysics 2024Quote: ... Cell membranes were stained with 0.1X CellMask™ Deep Red Plasma Membrane Stain (Invitrogen, Cat. No. C10046) at 37 °C and 150 rpm for 10 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... Equal loading was checked by taking images of the stained PVDF membranes with Pierce Reversible Protein Stain Kit (#24585, Thermo Fisher).
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bioRxiv - Cancer Biology 2022Quote: ... Migrating cells on the lower side of the membrane were fixed and stained with the Hema 3 Staining kit (Thermo Scientific). Migrating cells in each well were counted in five random fields by contrast microscopy using an Eclipse E200 Nikon microscopy (4X magnification ...
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bioRxiv - Microbiology 2021Quote: Cytoplasmic and membrane fractions from non-infected and Lm-infected cells were obtained using the Subcellular Protein Fractionation kit (Thermo Scientific). Cell samples and homogenized spleens were diluted in Laemmli buffer ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with the appropriate HRP-conjugated secondary antibodies and developed using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific).
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bioRxiv - Zoology 2021Quote: ... The membrane was incubated with a streptavidin-horseradish peroxidase conjugate and was detected using the ECL Western Blot Kit (Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ER and cytosol fractions from rPECs were prepared using the Mem-PER Plus Membrane Protein Extraction Kit (89842, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Neuroscience 2023Quote: Mouse embryonic fibroblast (MEF) cell lysates were processed using the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher, 89842) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: Plasma membrane proteins on the external side of the membrane were labeled using the LIVE/DEAD Fixable Red Dead Cell Stain kit (Invitrogen, #L23102), which labels free amine groups ...
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bioRxiv - Synthetic Biology 2021Quote: ... protein residue level was below the minimum detection threshold of Micro BCA Protein Assay Kit (Thermo Fisher, Cat. # 23235), and no bacterial colonies formed in bioburden detection.
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of Cetuximab was calculated using a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was taken and total protein concentration was determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). 35 μl of extract (about 4 mg/ml protein ...
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bioRxiv - Cancer Biology 2021Quote: We performed apoptosis assays using the annexin-V FITC/PI kit manual (catalog# P50-929-7; Thermo Fisher Scientific). Cells treated with chemotherapeutics were resuspended in fresh medium and plated in a T-75 flask at 37 °C for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations of the cell and tissue lysates were determined using BCA protein assay kit (Pierce, Thermo Fisher Scientific). Lysates were subjected to SDS-PAGE and proteins were transferred onto a nitrocellulose membrane (Biorad) ...
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bioRxiv - Cell Biology 2020Quote: ... Following determination of the protein concentration in the supernatants (Pierce™ BCA Protein Assay Kit; Thermo Fisher Scientific, 23250), samples were prepared for denaturing gel electrophoresis by addition of Laemmli Sample Buffer (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... according to manufacturer’s instruction and subsequently quantified with the Qubit 3.0 Fluorometer and the Qubit RNA BR Assay Kit (Thermo Fisher). RNA-seq libraries were generated with the Ovation Drosophila RNA-Seq System (NuGEN ...
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bioRxiv - Cell Biology 2020Quote: ... washed three times with PBS and processed using the Click-iT EdU594 Flow Cytometry Assay Kit (Thermo Fisher, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The quality score and quantity of purified cDNA library was assessed with Qubit dsDNA HS Assay kit (Life technologies) and Bioanalyzer High Sensitivity DNA assay (Agilent) ...
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bioRxiv - Immunology 2021Quote: ... microbial DNA was extracted from fecal pellets and quantified using Qubit 4 fluorometer and HS Assay Kit (Thermofisher Scientific). DNA libraries were prepared using the Nextera XT DNA Library Preparation Kit and Nextera Index Kit (Illumina ...
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bioRxiv - Genomics 2021Quote: ... The quantity and quality of total RNA samples were measured using Qubit RNA BR Assay Kit (Thermo Fisher Scientific) and Agilent TapeStation 4200 with RNA-specific tapes (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... the amounts of RNA and final libraries were quantified by Qubit 3.0 Fluorometer with the Qubit RNA HS Assay Kit (Invitrogen) and the Qubit dsDNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein concentration of the RIPA-soluble fraction was measured using a BCA protein assay kit (Thermo Fisher Scientific). The protein concentration of the urea-soluble fraction was assumed to have the same ratio as the RIPA-soluble fraction ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labeled antisense RNA probes for in situ hybridization (ISH) assays were synthesized from these plasmids in vitro according to manufacturer’s protocols (MEGAscript Kit; Ambion).
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bioRxiv - Evolutionary Biology 2020Quote: ... total RNA was assayed for quantity and quality using the Qubit® RNA BR Assay kit (Thermo Fisher Scientific) and RNA 6000 Nano Assay on a Bioanalyzer 2100 (Agilent) ...
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bioRxiv - Genomics 2021Quote: ... at a 2:1 ratio of beads to sample volume and the yields were measured using the Qubit dsDNA HS Assay Kit with a Qubit 3.0 fluorometer according to the manufacturer’s instructions (Thermo Fisher).
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bioRxiv - Evolutionary Biology 2021Quote: ... we quantified DNA concentrations using a fluorometric Quant-iT™ high sensitivity dsDNA assay kit (Molecular Probes™ Q33120). To normalise concentrations among samples ...
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bioRxiv - Genomics 2020Quote: ... The purified library was quantitated using a Qubit 3.0 fluorometer and ds DNA HS Assay kit (Thermo Fisher Scientific). The size distribution of the circle-damage-seq library was determined using Bioanalyzer (Agilent ...
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bioRxiv - Physiology 2022Quote: ... The total protein concentration in the lysates was determined using a Pierce BCA Protein Assay kit (Thermo Scientific, 23225). A 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels was used ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was collected and protein concentration was measured using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, catalog #23227) and Gen5 3.04 software (BioTek) ...
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bioRxiv - Cancer Biology 2022Quote: Endogenous cholesterol levels in A172 and MGG152 cells were measured by Amplex Red Cholesterol Assay Kit (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from isolated lung ECs and quantified using Qubit RNA High Sensitivity Assay Kit (Thermofisher, Q32852). RNA integrity was determined using the Bioanalyzer RNA 6000 Nano assay kit (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... the Click-iT(tm) HPG Alexa Fluor(tm) 488 Protein Synthesis Assay Kit was used (Thermo Fisher Scientific, C10186).
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bioRxiv - Molecular Biology 2020Quote: ... The concentrations of the purified fragments were measured using Qubit ds DNA HS Assay Kit with a Qubit 3.0 fluorometer (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... The total amount of protein was quantitated by using Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Scientific). Equal amounts of proteins were resolved on 10 or 15% Tris-glycine SDS-PAGE under reducing conditions and transferred onto Immobilon-P Membrane ...
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bioRxiv - Molecular Biology 2020Quote: ... EdU staining was performed using a Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified PCR amplicons were quantified using the Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen Ltd, Carlsbad, USA), diluted and pooled together into 75 µL comprising 1.5 ng/μL of DNA final concentration before shipping for sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to a fresh tube and the concentration was determined using Qubit (Qubit dsDNA HS Assay Kit, Thermo Fisher) and qPCR (KAPA SYBR FAST qPCR Master Mix ...
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bioRxiv - Cell Biology 2022Quote: Total protein was quantified for all samples using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific 23225) as per the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ISH on formalin-fixed paraffin-embedded sections was performed with a ViewRNA ISH Tissue Assay Kit (Thermo Fisher Scientific) with gene-specific probe sets for human NR5A1 ...
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bioRxiv - Immunology 2022Quote: ... Cells were first stained with surface antibodies and then EdU incorporation was detected using the Click-iT Plus EdU Flow Cytometry Assay Kit according to the manufacturer’s instructions (ThermoFisher). Samples were then acquired on a Becton Dickinson LSR II Flow cytometer and analyzed using FlowJo ...
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bioRxiv - Genomics 2020Quote: ... Total protein present in the supernatants were quantified using Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). Relative amounts of toxin were normalized to total proteins.
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bioRxiv - Genomics 2020Quote: ... cDNA quantity and quality were evaluated using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, Erembodegem-Aalst, Belgium). Size distribution of our library was determined using an Agilent 2100 Bioanalyser ...
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bioRxiv - Genomics 2020Quote: ... Library concentrations were determined using Qubit Fluorometer (v.4.0) and the Qubit ds(DNA) HS Assay Kit (Life Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... Supernatants were harvested and the protein concentration was measured using the Pierce BCA protein assay kit (ThermoFisher Scientific, 23225). Samples were mixed with NuPAGE LDS sample buffer (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Following reverse transcription cDNA was purified and quantified using a Qubit ds DNA HS Assay kit (Thermo Fisher Scientific) and a cDNA library prepared using Nextera XT DNA Sample Preparation Kit (Illumina) ...
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bioRxiv - Physiology 2020Quote: Caspase-3-like activity was quantified using the EnzChek Caspase-3 Assay kit #1 (Molecular Probes, Eugene, OR, USA). Tissue samples were thawed on ice for 5 min ...