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Citations for Thermo Fisher :
6701 - 6750 of 10000+ citations for Anti NKCC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated 1 hour in secondary antibodies: goat anti-rabbit or goat anti-mouse AlexaFluor488/546 (1:1000; Invitrogen Life Technologies, Carlsbad, CA). Sections were mounted (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... slides were washed five times in 0.01% TBST and covered with TBST containing appropriate secondary antibody in 1:300 dilution (goat anti-mouse IgG Alexa Fluor 488, goat anti-rabbit IgG Alexa Fluor 594 (Invitrogen, Carlsbad, CA, USA)) and incubated for 40 min at RT in the dark in a humidified chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... and secondary antibodies (1:500 goat anti-mouse Alexa Fluor 488, Fisher Scientific A11008 / goat anti-rabbit Alexa Fluor 546, Fisher Scientific A11035) for 1 hour at room temperature.
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bioRxiv - Developmental Biology 2024Quote: ... the primary neurons were incubated with secondary antibodies (anti-mouse-568, 1/1000, Molecular probes A11019; anti-rabbit-488, 1/1000, Molecular probes A11008), sir-actin (1/2000 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were immunostained with an anti-EBOV GP mouse antibody (IBT, MD, USA) and Alexa Fluor 488 anti-mouse secondary (Thermo Fisher, MA, USA). Nuclei were counterstained with Hoechst 33342 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed and anti-rabbit Alexa Fluor 488 and anti-mouse Alexa Fluor 594 secondary antibodies were added (Thermo Fisher, MA, USA). After 1-2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBST and incubated in secondary antibodies in PBST (1:500 goat anti-rabbit AlexaFluor 555/488 or goat anti-chicken AlexaFluor 488, Thermo Fisher Scientific, USA). Following final rinses with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... We visualized immunoreactive complexes using anti-rabbit or anti-guinea pig secondary antibodies conjugated to Alexa-488 and -647 fluorophores (1:1000 for both, Invitrogen, Waltham, MA, USA).
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were then incubated for two hours in secondary antibodies – goat anti-mouse 568 (Thermo Fischer, A-11031) and goat anti-chicken 488 (Thermo Fisher, A-11039), followed by coverslipping with ProLong Gold with DAPI for nuclear labeling (Thermo Fischer ...
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bioRxiv - Cell Biology 2023Quote: ... Basal bodies were identified by the decoration of the primary cilium with anti-acetylated tubulin antibodies followed by goat anti-mouse-IgG-Dylight 488 (#35503, ThermoFisher Scientific, Waltham, USA).
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bioRxiv - Systems Biology 2023Quote: ... were used at a concentration of 1:100 then stained with anti-rabbit-PE or anti-mouse Pacific Blue secondary antibody (BioLegend® #406421, Life Technologies #P10993) for flow cytometry.
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bioRxiv - Systems Biology 2023Quote: ... coverslips were incubated with Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse secondary antibodies (Thermo Fisher, A11034 and A11005, respectively) and Hoechst nuclear stain (1:2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were washed with TBST followed by incubation with the secondary antibody such as anti-mouse FITC-488 (1:1000) or goat anti-rabbit Alexa Fluor-647 (Life Technologies, Thermo Scientific, USA) at room temperature for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies used were goat anti-rat Alexa Fluor 488 or goat anti-mouse Alexa Fluor 596 (Thermo Fisher scientific, 1:1000). The incubation with antibodies was accomplished in a humidified chamber for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and then with Alexa Fluor 594-conjugated donkey anti-goat IgG (H+L) and Alexa Fluor 488-conjugated donkey anti-mouse IgG (H+L) antibodies (Invitrogen; cat # A-21202). The stained sections were preserved in Vectashield antifade mounting medium with DAPI (Vector Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were incubated with Anti-Collagen I and Anti-Collagen IV antibodies (Catalog# PA5-29569 and # MA1-22148, Thermo Fisher Scientific, Waltham, MA) diluted 1:100 in ICC buffer ...
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bioRxiv - Cell Biology 2023Quote: ... wells were briefly washed with PBS and secondary antibodies (Donkey Anti-Rabbit 555, Invitrogen, A32794, 1:1000; Donkey Anti-Mouse 647, Invitrogen, A32787, 1:1000) were diluted in PBS and added to wells to incubate for 1 hour at room temperature while covered from light ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... Brain slices were then incubated for 20 h at RT with primary antibodies (chicken anti-GFP, 1:1000, ab13970, Abcam; rat anti-mcherry, 1:1000, Thermo Fisher Scientific, M11217) in PBS-based blocking solution containing 0.1% TX ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were washed 3 x 5 min with TBSB and incubated with conjugated anti-HRP (goat mAb, JacksonImmuno; 1:500) and secondary antibodies (Alexa Fluor 647 Donkey anti-mouse, Invitrogen at 1:500) for 2 hours at RT and washed with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... followed by secondary antibodies (donkey anti-rabbit IgG Alexa Fluor 488, donkey anti-mouse IgG Alexa Fluor 568, Thermo Fisher Scientific, A21206, A10037) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 1 hour in a solution containing donkey anti-rabbit and donkey anti-mouse antibodies conjugated with Alexa Fluor 555 and Alexa Fluor 647 (1:200; Invitrogen, RRID:AB_2762834 and RRID:AB_162542), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 1 hour sat room temperature with the following secondary antibodies: anti-goat anti-human Alexa Fluor 647 (Invitrogen, Carlsbad, California, USA). All washing steps were performed with PHEM-T buffer ...
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bioRxiv - Cell Biology 2024Quote: Secondary antibodies used are: donkey anti-mouse or donkey anti-rabbit coupled to Alexa Fluor 594 or 647 (Invitrogen, ThermoFisher Scientific; 1:1,000). To amplify the GFP fluorescence intensity post-fixation ...
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bioRxiv - Cell Biology 2024Quote: Secondary antibodies used are: donkey anti-mouse or donkey anti-rabbit coupled to Alexa Fluor 594 or 647 (Invitrogen, ThermoFisher Scientific; 1:1,000). To amplify the GFP fluorescence intensity post-fixation ...
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bioRxiv - Cell Biology 2024Quote: ... 1/200) and secondary antibodies were Donkey anti-rat Cy3 (Jackson 712-165-150 ; 1/500) Donkey anti-rat AlexaFluor488 (Invitrogen A21208 ; 1/500) and Donkey anti-mouse Cy3 (Jackson 715-165-150) ...
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bioRxiv - Developmental Biology 2024Quote: ... The pair of secondary antibodies used were anti-chick and anti-mouse conjugated either with AlexaFluor Plus 488 (1:200 dilution; A32931; Invitrogen, Carlsbad, CA, USA) and AlexaFluor Plus 555 (1:200 dilution ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were blocked in 6% BSA and immunolabeled with anti-α3 antibody (mouse Anti-ATP1A3 xVIF 9-G10, MA3-915, Invitrogen, Thermo Fisher scientific) for 2 hours 200 times in PBS1x containing 1%BSA or at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies: Alexa Fluor 633 goat anti-mouse (1:400 for anti-TH, 1:200 for nc82; A-21052; Invitrogen, Waltham, MA, USA).
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bioRxiv - Neuroscience 2021Quote: ... and the secondary antibody goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor 568 (1:500, Thermo Fisher Scientific, Cat# A-11011). Antibodies were stored and prepared as recommended by the manufacturer and did not undergo repeated freeze/thaw cycles ...
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bioRxiv - Molecular Biology 2020Quote: Re-suspend cells in 100 μl of diluted secondary antibody (in this case Alexa Fluor 488 donkey anti-rabbit antibody, ThermoFisher Scientific A21206, diluted 1:1000) in 1% BSA in PBS with 1:25 RNasin Plus ...
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bioRxiv - Evolutionary Biology 2019Quote: ... samples were washed three times with 1x PBST and then incubated for 45 min with secondary antibody (Alexa488® Goat Anti-Rabbit IgG Antibody; Life Technologies, 1:500 dilution), plus Alexa Fluor568® Phalloidin (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... 300 μl of CHAMP buffer containing 4 nM Alexa488-conjugated anti-Flag antibody (Alexa Fluor 488 antibody labeling kit, Thermo Fisher; monoclonal BioM2, Sigma-Aldrich) was injected to wash off unbound RNP and label DNA-bound RNP complex ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were then washed once with PBS and incubated in primary antibody overnight at 4°C with the following antibodies: anti-MAP2 (1:3000, Thermo Fisher Scientific cat. # PA1-10005), TH (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... the slides were incubated in secondary antibody (Alexa Fluor 488 donkey anti-mouse antibody, 1:200, product #R37114, Molecular Probes-Life Technologies, Eugene, OR, USA) for 4 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... the slides were incubated in secondary antibody (Alexa Fluor 488 donkey anti-mouse antibody, 1:200, product #R37114, Molecular Probes-Life Technologies, Eugene, OR, USA) for 4 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... neuronal bodies and projections were labelled with antibodies against MAP2 (Thermo Fisher, dilution 1:1000, secondary antibody: goat anti-rabbit Alexa Fluor 647, Life Technologies, dilution 1:1000) and Tuj1 (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed with PBS for 5 minutes and incubated for 30 minutes with the following secondary antibodies: goat anti-rabbit AlexaFluor 488 (Invitrogen Antibodies, A-21207, 1: 500) and goat anti-mouse AlexaFluor 594 (Invitrogen Antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed in antibody dilution buffer and incubated with Alexa Fluor 594 conjugated goat anti-rabbit IgG secondary antibody (1:500; Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at 28°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then stained with mouse monoclonal anti-CFTR antibody 570 (Cystic Fibrosis Foundation; 1:200 in above blocking buffer) and rabbit polyclonal anti-ZO-1 antibody (ThermoFisher Scientific #40-2200 1:200) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Surface CRT was detected using anti- CRT(N) or anti-CRT(Cmut) antibody (1:200 dilution) followed by staining with Alexa Fluor™ 647 conjugated secondary antibody (Invitrogen A21245, 1:2000 dilution). Total CRT in healthy donor platelets was stained with anti-CRT(N ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies and concentrations were used: Alexa 488- and Alexa 594-conjugated goat anti mouse and rabbit antibodies (Thermo Fisher Scientific, IF 1:500), and peroxidase-conjugated goat anti-mouse and rabbit antibodies (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated overnight at 4 °C with primary antibodies: rabbit anti-phospho-EGFR (Y845) polyclonal antibody (44-784G, Thermo Fisher Scientific, diluted 1:200); Purified Mouse anti-E-Cadherin monoclonal antibody (Clone 36 ...
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bioRxiv - Biophysics 2024Quote: ... Brain slices were washed three times in PBS and incubated with a secondary antibody (Alexa Fluor 546 conjugated anti-rabbit goat antibody, Thermo Fisher Scientific, Waltham, MA, USA) in antibody dilution solution for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then washed three times followed by an incubation for 1 h with the following secondary antibodies: goat anti-rat Alexa Fluor 647 antibody (Thermo Fisher, #A78947, 1 µg/ml), goat anti-mouse Alexa Flour 488 antibody (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the mouse transduced intranasally with 5×1010 of Ad5-hACE2 were used as negative control ...
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bioRxiv - Biophysics 2021Quote: ... followed by secondary staining with an Alexa Fluor 647 goat anti-rabbit antibody (ThermoFisher, A-21245) at 1:1000 dilution in 1% BSA for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1 hour of incubation with the secondary anti-rabbit Alexa488 antibody (Molecular Probes, Invitrogen). The cells were mounted and viewed by fluorescence microscopy.
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1 hour of incubation with the secondary anti-rabbit Alexa488 antibody (Molecular Probes, Invitrogen). The cells were mounted and viewed by fluorescence microscopy.
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MYB insufficiency disrupts proteostasis in hematopoietic stem cells leading to age-related neoplasiabioRxiv - Cancer Biology 2021Quote: ... Cells were then stained with eFluor 450-conjugated anti-Ki67 antibody (clone SolA15, ThermoFisher, Waltham MA) and then analysed by flow cytometry.
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bioRxiv - Bioengineering 2021Quote: ... and with an Alexa Fluor 647 goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher). After washing ...