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Citations for Thermo Fisher :
6651 - 6700 of 7736 citations for Recombinant Human SRPK1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: The COVID-19 receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using HEK293F cells (Invitrogen). The COVID-19 RBD (residues Arg319-Phe541 ...
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bioRxiv - Immunology 2021Quote: ... as a Cas9-RNP complex (1:1 Cas9:gRNA molar ratio) were delivered primary human CD3+ T cells after 2 days of Anti-CD3/-CD28 Dynabead (ThermoFisher) stimulation ...
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bioRxiv - Microbiology 2021Quote: ... Peptide identification of the human and SARS-CoV-2 proteome was performed by Proteome Discoverer (version 2.2, Thermo Fisher Scientific) using the SequestHT search engine as described (Seidel et al 2018) ...
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bioRxiv - Immunology 2020Quote: ... T cells are enumerated using Trypan Blue exclusion and co-incubated with CD3/CD28 Human T cell Activating Dynabeads (ThermoFisher) at a 3:1 bead:T cell ratio in a total volume of 1 ml TCM supplemented with 50 units/ml recombinant IL-2 in a 48-well plate ...
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bioRxiv - Cell Biology 2021Quote: To prepare the probe for hybridisation approximately 200-600ng of labelled probe DNA was ethanol precipitated with the 5 µg of sheared salmon sperm DNA and 5 µg of mouse or human Cot1 DNA (both from Invitrogen) and resuspended in hybridisation buffer (50% formamide ...
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bioRxiv - Cell Biology 2021Quote: ... For spindle length and chromosome alignment analyses the following secondary antibodies were used at 1:500 dilution: Goat anti-Human IgG conjugated to Alexa Fluor 488 (A11013; Invitrogen), Goat anti-Mouse IgG conjugated to Alexa Fluor 647 (A21236 ...
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bioRxiv - Cell Biology 2021Quote: U-2 OS cells (human bone osteosarcoma epithelial cells, ATCC Number: HTB-96) (88) were grown in D-MEM Glutamax (Gibco) supplemented with final concentrations of 10% fetal calf serum (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... we stimulated these CD4+ T cells with CD3/CD28 crosslinking for 45 hours (Gibco, Dynabeads Human T-Activator CD3/CD28). We then performed ChIP-seq (2 million stimulated CD4+ T cells) ...
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bioRxiv - Cell Biology 2021Quote: Human epithelial amnion cells (AV3 cells, ATCC-CCL21; FL, ATCC-CCL62; WISH, ATCC-CCL25) were cultured in DMEM/F12 (Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... falciparum cell lines were cultured in vitro in human erythrocytes (UK Blood and Transfusion Service) at 37°C in RPMI-1640 (Gibco) based media supplemented with 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... fresh human pancreatic islets preparations were resuspended in CMRL culture medium (cat. 11-530-037, Fisher Scientific, Pittsburgh, PA, USA) supplemented with L-Glutamine 1% (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... and enriched for CD8+ T cells with the MagniSort™ Human CD8+ T cell Enrichment Kit (#8804-6812-74, Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: The pulmonary HLA-DR and CD3-positive cells were isolated using superparamagnetic beads coupled with anti-human CD3 and Pan Mouse IgG antibodies (Invitrogen Dynabeads® ...
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bioRxiv - Microbiology 2021Quote: Human endothelial kidney (HEK 293) and murine fibroblast (3T12) cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Life Technologies) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Real-time quantitative RT-PCR (qRT-PCR) was performed to assess the expression of human PIK3CD (Hs00192399_m1) using the TaqMan gene expression assay (Applied Biosystems) while GAPDH was used as reference (Hs99999905_m1) ...
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bioRxiv - Immunology 2022Quote: One million cells PBMCs were incubated in 24 well plates with 30 IU/mL IL-2 (NCI) and with or without 25 μL of Human T-Activator CD3/CD28 dynabeads (ThermoFisher). Following 48 hours ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed four times with washing buffer followed by incubation with HRP-conjugated anti-Human IgG secondary Abs (Invitrogen) (prepared in a diluted solution of blocking buffer [0.4% BSA] ...
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bioRxiv - Neuroscience 2022Quote: ... 50 and 35µg of proteins (for cell lysates, mouse brains and human cortices, respectively) were added with 1x Sample buffer (Thermo Fisher) and 1x Sample Reducing Agent (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... Kits and murine and human serum CD40L was quantified using the commercially available soluble CD40L ELISA Kits (Thermo Fisher Scientific), following the manufacturer’s procedure.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with corresponding secondary antibodies for 1 hr at room temperature: goat anti-human-Alexa-488 and goat anti-mouse-Alexa-488 (Invitrogen) for 293T internalization studies and goat anti-rabbit-Alexa-488 and goat anti-human-Alexa-555 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... 60 mm dishes of BHK-21 cells were transiently transfected with 2.5 μg human ACE2 or no DNA for control using standard lipofectamine 2000 (Life Technologies) protocols ...
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bioRxiv - Biochemistry 2022Quote: ... containing N-terminal ten histidine tag (His-tag) and human rhinovirus 3C (HRV 3C) protease site was amplified by PCR and cloned into the pFastBac vector (Invitrogen) using seamless cloning method ...
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bioRxiv - Neuroscience 2022Quote: ... Affymetrix Human Transcriptome Array 2.0 ST arrays were hybridized according Affymetrix recommendations using the Ambion WT protocol (Life technologies, France) and Affymetrix labelling and hybridization kits ...
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bioRxiv - Cell Biology 2022Quote: ... cells were then incubated with pre-warmed DMEM containing 20 µg/ml of TxR-Tfn (from human serum, Molecular Probes) and 0.1 % BSA for the specified times ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted from rat glomeruli tissue and human podocyte samples using the mirVanaTM Isolation Kit (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Human CPα (CAPZA1, cloneID 3047937) and CPβ (CAPZB, clone ID 6715709) were purchased from Dharmacon and inserted into pcDNA3 (Invitrogen) with N-terminal MYC or FLAG tags ...
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bioRxiv - Cell Biology 2022Quote: Cryo-preserved primary human hepatocytes (PHH) from single donors (BD Gentest, Tewksbury, MA; Lonza, Walkersville, MD; and Thermo Fisher Scientific) were used for all in vitro experiments ...
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bioRxiv - Immunology 2022Quote: ... in culture media or stimulated for 48h with α-CD3/CD28 coated beads (Dynabeads Human T-Activator, Thermo Fisher scientific) in a 1:1 ratio (beads to cells) ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF-7 human breast cancer cells were obtained from ATCC and cultured in Roswell Park Memorial Institute medium (RPMI-1640; Gibco) supplemented with penicillin/streptomycin and 10% FCS ...
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bioRxiv - Cancer Biology 2022Quote: ... total cell RNA was extracted from FACS-purified LP or HP cell populations from B16-F10 murine or LM-MEL-28 human melanoma cells using PureLink RNA Mini kits (ThermoFisher), including on-column DNase treatment ...
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bioRxiv - Biophysics 2022Quote: ... polyclonal anti-human MPO (IgG, sheep, 1:50) (AA16-718, Antibody online), monoclonal anti-human Histone H1 (IgG2a, mouse, 1:100) (MA5-13750, Invitrogen), or monoclonal anti-human NPM1 (IgG1 ...
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bioRxiv - Cell Biology 2022Quote: ... >1.5 kb 5’and 3’ homology arms were amplified from genomic DNA isolated from one 6-cm dish of immortalized human retinal pigment epithelial cells (hTERT-RPE1 cells, ATCC) using a Purelink Genomic DNA Mini Kit (ThermoFisher). The π-element was PCR amplified from plasmid #1 ...
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bioRxiv - Cell Biology 2022Quote: Toxoplasma gondii RH∆hxgprt strain was maintained by growth in confluent primary human foreskin fibroblasts (HFFs) in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... the pFastBac plasmid containing full-length human dynein or tail-truncated dynein was transformed into DH10Bac competent cells (Gibco, #10361012) with heat shock at 42 °C for 45 seconds followed by incubation at 37 °C for 4 hours in S.O.C ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... were designed and used to create three different human-yeast hybrid genes by PCR (AccuPrime Pfx DNA polymerase from Invitrogen). The primers also add the attB1 and attB2 sites at the 5’ and 3’ ends of the PCR for cloning in pDONR221 entry clones using the BP cloning strategy ...
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bioRxiv - Immunology 2022Quote: ... freshly isolated or cryopreserved human PBMC or purified CD8+ T-cells (1×106) were cultured in complete culture medium (RPMI 1640 (Gibco) supplemented with 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Immunology 2022Quote: ... Sorted Treg cells from HD and IPEX patients were stimulated with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Immunology 2022Quote: ... stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel, ThermoFisher) per manufacturer recommendation ...
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bioRxiv - Genomics 2022Quote: Purified human and bacterial DNA were quantified for concentration with the Qubit dsDNA HS assay (Life Technologies, Q33231, MA, USA), and pooled by adding 94% of human DNA ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone) primary mAb and FITC goat anti-mouse secondary antibody (Invitrogen). The cells were then fixed using 4% paraformaldehyde before staining the nuclei with Hoescht 33342 (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: Telomerase-transduced Human Foreskin Fibroblasts (HFFs) and MRC5 lung fibroblasts were cultured in Dulbecco’s modified Eagle serum (DMEM; Invitrogen #11965118) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2022Quote: ... then co-cultured at the indicated ratios and activated with Dynabeads Human T-Expander (1 bead:16 CD3+ T cells; Gibco) for 4 days ...
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bioRxiv - Synthetic Biology 2022Quote: ... cryopreserved PBMCs or T cells were thawed on day 0 and cultured with Human T-Activator αCD3/CD28 Dynabeads (Gibco) at 3:1 bead:cell ratio in AIM-V media (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... CVCL_1926) and HEK293T cells stably expressing human ACE2 (BEI NR-52511, RRID: CVCL_A7UK) were maintained in DMEM (Gibco, 11965-092) with 10% FBS (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with the SARS-CoV-2 wild type (isolate SARS-CoV-2/human/SWE/01/2020; Genbank accession: MT093571) in VP-SFM (Gibco). The virus was harvested at day three ...
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bioRxiv - Molecular Biology 2022Quote: ... and 24 hours later cells were transfected with the Bluescript vector (empty vector control) and Human L1 plasmid (used in L1 retrotransposition assay) using Lipofectamine 2000 reagent (Invitrogen). Cells were harvested 36 hours after transfection ...
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bioRxiv - Molecular Biology 2023Quote: SF268 (human glioblastoma) cells were cultured at 37 °C with 5% CO2 in RPMI medium supplemented with 10% FBS (Gibco). Vero (African green monkey kidney ...
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bioRxiv - Synthetic Biology 2023Quote: ... Syncytin1 was recoded based on the amino acid sequence from the The Human Protein Atlas and ordered as a gBlock (ThermoFisher). PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme ...