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Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations for 2 4 Difluoro N 2 methoxy 5 4 4 morpholinyl 6 quinazolinyl 3 pyridinyl benzenesulfonamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were fixed with 4% PFA/PBS (Thermo Scientific/ Life Technologies, Paisley, UK), permeabilised with 0.1% Triton-X100 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were fixed with 4% PFA/PBS (Thermo Scientific/ Life Technologies, Paisley, UK), permeabilised with 0.1% Triton-X100 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Denatured protein lysates were resolved on 4-12% Bis-Tris gels (Invitrogen, NP0335BOX) and transferred to PVDF membranes (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Human colonoids embedded in BME were fixed in 4% formaldehyde (28908, Thermo Fisher) for 45 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... lysates were mixed with 4× NativePAGE sample buffer and Coomassie G-250 (ThermoFisher) and resolved using NativePAGE 3–12% gels ...
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bioRxiv - Biochemistry 2024Quote: ... using 4-12% Bolt Bis-Tris Plus Mini protein gels (Thermo Fisher/Invitrogen) stained with Quick Coomassie Stain (ProteinArk) ...
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bioRxiv - Biochemistry 2024Quote: ... using 4-12% Bolt Bis-Tris Plus Mini protein gels (Thermo Fisher/Invitrogen) stained with Quick Coomassie Stain (ProteinArk) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by fixation with 4% formaldehyde solution (ThermoFisher, Image-iT™ Fixative Solution) for 15min ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were run on a NuPAGE 4-12 % Bis-Tris gel (ThermoFisher Scientific) and transferred to a PVDF membrane using a Turbo-Blot transfer system (Biorad) ...
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bioRxiv - Biochemistry 2024Quote: ... ∼90% confluent cells were either treated 4 h with HBSS media (ThermoFisher, 24020117) with or without 50 μM Bafilomycin (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... and/or pCMV-HA-Ubiquitin plasmids for 4 h in OptiMEM (ThermoFisher, 31985062) and 1 μg/ml PEI (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and mounted with Cytoseal mounting medium (Thermo Fisher Scientific, catalog no. 8312-4). Images were recorded on a Zeiss Axioskop microscope (RRID ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were run on NuPage 4- 12 % SDS-PAGE gel (Invitrogen # NP0321BOX) using MOPS buffer and transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... for 24 hours and then stored in 100% methanol (Fisher Scientific A412P-4).
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bioRxiv - Cancer Biology 2024Quote: ... before being fixed for 15 minutes (RT) with 50μL 4% PFA (ThermoFisher, 11586711) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal protein amounts (20 μg) were electrophoresed on 4-12% polyacrylamide gels (Invitrogen) and transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... The selected cells were treated with 4 μg/mL doxycycline (cat# AAJ6267106, ThermoFisher) for 72 h ...
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bioRxiv - Developmental Biology 2024Quote: Embryos at desired stages were fixed with 4% paraformaldehyde (Methanol-free, Thermo Fisher) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.05 μg/ml of EGF in 4-well Nunc plates (Thermo Fisher Scientific). Based on the condition tested ...
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bioRxiv - Cell Biology 2024Quote: ... Quality and concentration of libraries were determined using a Qubit 4 Fluorometer (Invitrogen) and a 2100 Bioanalyzer Instrument (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... and 4 µg/ml goat anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific) in PBT ...
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bioRxiv - Genetics 2024Quote: ... RNA was quantified using a Qubit 4 fluorometer (ThermoFisher Scientific, Waltham, MA, USA). RNA integrity (RIN ...
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bioRxiv - Cell Biology 2021Quote: ... The insoluble fraction was removed by high-speed centrifugation at 4°C (25658 x g for 1 hour, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was incubated with 2 ml Ni-NTA resin (Thermo SCIENTIFIC #88222 ...
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bioRxiv - Immunology 2022Quote: ... The cell suspension was centrifuged at 500 g for 5 min at 4°C and the cell pellet was incubated with ACK lysing buffer (Thermo Fisher Scientific, A1049201) for 5 minutes to lyse the red cells ...
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bioRxiv - Molecular Biology 2022Quote: To control efficiency of the enzymatic treatment 5 μg of the enzyme-treated products or controls were run on a NuPage™ 4-12 % Bis-Tris gel (Invitrogen, Carlsbad, USA) at 100 V for 60 minutes using the EI9001-XCELL II Mini Cell (Novex ...
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bioRxiv - Microbiology 2021Quote: ... murine PBMCs were stimulated for 4 hrs with spike (5 μg/mL) and then supplemented with Protein Transport Inhibitor cocktail (Life Technologies, California, USA) for a further 10-12 hrs ...
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bioRxiv - Biochemistry 2021Quote: Protein samples were heated for 5 min at 95 °C and separated by electrophoresis in NuPAGE™ 4-12 % gels (ThermoFisher, Cat# NP0329BOX). RNA samples were heated for 2.5 min at 95 °C and separated by electrophoresis in denaturing 9 % acrylamide (19:1 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated at room temperature for 4-5 h in Triton PBS containing Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:1000; A-21207, Thermo Fisher Scientific, RRID:AB_141637). After binding of streptavidin/antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded 4–5 × 104 cells per well in 8-well Lab-Tek II chambered cover glass slides (Nunc, Rochester, NY, USA) and incubated overnight ...
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bioRxiv - Biophysics 2020Quote: ... 20 μL of protein solution was incubated with 4 μL of LDS sample buffer and incubated at 100°C for 5 mins before 10 μL was loaded on a 4-12% bis-tris gel (NuPAGE™, Thermo Fisher Scientific). The gel was either stained with Coomassie blue or transferred to a 0.22 μm polyvinylidene fluoride (PVDF ...
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bioRxiv - Systems Biology 2022Quote: ... and the resulting supernatant (100 ng/ml) was incubated for 4 h at 37°C together with 5 mM glutamine (Gibco, Cat# 25030-081), 1 mM phosphoribosyl pyrophosphate (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The in vitro transcription reaction for the 5’ cap driven Fluc reporter included a 1:4 ratio of Invitrogen™ Anti-Reverse Cap Analog (Fisher Scientific AM8045) and GTP solution (0.4 µl cap analog to 1.6 µl of GTP solution) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell suspensions were placed in clean cytospin cones and spun at 600g for 5 min on an Epredia Cytospin 4 (Fisher Scientific Cat#A78300003) to charged glass slides ...
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bioRxiv - Microbiology 2021Quote: ... murine PBMCs were stimulated for 4 hrs with SpK (5 μg/mL) and then supplemented with Protein Transport Inhibitor cocktail (Life Technologies, California, USA) for a further 10-12 hrs ...
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bioRxiv - Molecular Biology 2020Quote: ... To clear the homogenate from debris and larger worm fragments it was centrifuged at 800 g for 5 minutes at 4°C (Heraeus Fresco 21, Thermo Scientific, Langenselbold, Germany) and the supernatant collected ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed thrice with sterile PBS and 200 μL of cell suspension were cytospined for 5 min (Shandon Cytospin 4, Thermo Scientific, Cheshire, UK). The identification of infected DPSCs was carried by Gimenez staining.
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bioRxiv - Immunology 2021Quote: ... Antibodies were harvested from cell supernatants at 4 to 5 days post transfection and purified using CaptureSelect™ LC-lambda (mouse) Affinity Matrix (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: 3D caecaloids grown in expansion medium for 4-5 days after passaging were dissociated into single cells by TrypLE Express (Gibco Thermo Fisher Scientific) digestion ...
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bioRxiv - Cancer Biology 2020Quote: ... Dissociated tumor cells from Lcre;NICD1 animals treated with IMR-1 or vehicle were resuspended in 0.2 ml cold phosphate-buffered saline (PBS) and spun onto a slide at 800 g for 5 min using a Shandon Cytospin 4 Cytocentrifuge (Thermo Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stained for 15min at 4 °C in Flow buffer (PBS with 5% cosmic calf serum) containing the following antibodies: CD11b-PE (Invitrogen, 12-0112-82), CD45-APC (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... at 300 x g for 5 min at 4°C and resuspended in 50 μl of unlabeled anti-FCGR3/CD16-FCGR2B/CD32 Ab (Invitrogen, 14-0161-86) 10 μg/ml in FACS-buffer (PBS ...
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bioRxiv - Plant Biology 2024Quote: ... annealing at 52ºC for 5 s and extension at 60ºC for 4 min using a GeneAmp® PCR System 9700 thermocycler (Applied Biosystems, Warrington, UK). Samples were Sanger-sequenced using a 3730 Series Genetic Analyzer Thermo Fisher Scientific UK Ltd (Loughborough) ...
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bioRxiv - Molecular Biology 2024Quote: ... Differentiation was induced at ∼85% confluency by switching to media containing 4:1 DMEM/M199 supplemented with 5% normal horse serum (Invitrogen; cat #: 26050-088). Following seven days of differentiation cells were treated with either ...
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bioRxiv - Molecular Biology 2023Quote: ... FUCCI positive cells were cultured and washed once with phosphate-buffered saline (PBS, 300 x g / 5 min) and fixed with 4% methanol-free formaldehyde (Thermo Fisher Scientific, 28906) for 20 min at room temperature ...
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bioRxiv - Systems Biology 2023Quote: Labeled blots were washed 4 x 5 min each then incubated in SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific™ #34577) at room temperature for 3 min ...
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bioRxiv - Physiology 2023Quote: ... Proteins were denatured at 95°C for 5 minutes and run on a Bolt™ 4-12% Bis-Tris polyacrylamide gel (Thermo Scientific #NW04125BOX). Proteins were transferred to a nitrocellulose membrane (Invitrogen #PB3310 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol, 50 mM maltose) and were concentrated using an Amicon Ultra Centrifugal Filter Unit (50 kDa, 4 ml) (Fisher Scientific, EMD Millipore). Proteins were further purified by gel filtration chromatography (Superdex-200 ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.005 % BPB) and incubated 5 min at 100 ºC followed by electrophoresis on Novex™ 4-20% Tris-Glycine gradient gels (Invitrogen, WXP42020BOX; WXP42026BOX). Proteins were transferred onto 0.2 µm nitrocellulose membranes (Pall Laboratory ...
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Optimisation of immunocytochemistry methodology for the detection of endogenous eIF2B localised focibioRxiv - Cell Biology 2022Quote: ... Cells were then washed with PBST 4 times for 5 min and mounted using ProLong™ Gold Antifade Mountant with DAPI 220 (Invitrogen, Thermo Fisher Scientific). Cells were viewed on a Zeiss LSM 800 confocal microscope.
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated over-night at 4°C with the following primary antibodies: mouse anti-claudin-5 (Invitrogen, USA, 1:100 dilution) and rabbit anti-occludin (Invitrogen ...