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Citations for Thermo Fisher :
6601 - 6650 of 7736 citations for Recombinant Human TNFRSF4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 (Apath) cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen, 10-013-CV); African green monkey kidney Vero (ATCC ...
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bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human or mouse ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously44 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were then washed and incubated with PE-conjugated goat anti-human Fc antibodies (Thermo Fisher 12-4998-82) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Freshly isolated HTII-280+ and HTII-280− cells derived from CD31−CD45−EpCAM+ cells of human distal lungs were resuspended in Advanced DMEM/F12 (Thermofisher) base medium supplemented with 10 mM Hepes (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: ... mosquitoes were offered a sterile bloodmeal consisting of 50 % human red blood cells (Etablissement Français du Sang de Guadeloupe-Guyane) and 50 % foetal bovine serum (Thermo Fisher). The bloodmeal was provided to mosquitoes in an autoclaved Hemotek reservoir (Hemotek ...
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bioRxiv - Biophysics 2021Quote: The immortalized hTERT RPE1 cells (Human Retinal Pigmented Epithelium) were cultivated in DMEM F12 without Phenol Red (Gibco, Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biophysics 2021Quote: The immortalized hTERT RPE1 cells (Human Retinal Pigmented Epithelium) were cultivated in DMEM F12 without Phenol Red (Gibco, Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: The template RNA for cDNA synthesis was isolated from human HEK293 cell culture with TRIzol reagent (Thermo Fisher Scientific, USA), or FFPE samples ...
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bioRxiv - Developmental Biology 2021Quote: ... embedded in GFR Matrigel, and cultured in human organoid expansion medium (Boj et al., 2015) (AdDMEM/F12 medium supplemented with HEPES (1x, Invitrogen), Glutamax (1x ...
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bioRxiv - Immunology 2021Quote: - IFNγ and IL-10 levels were measured in the supernatant of the WBA using IFN gamma and IL-10 Human ELISA Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Monocytes were then differentiated into macrophages by 7-9 days of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Immunology 2020Quote: ... Digested sera was probed with Rabbit anti-Human IgG-HRP H+L Secondary Antibody (ThermoFisher Scientific Catalog #A18903, 1:10,000), Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423 ...
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bioRxiv - Immunology 2020Quote: ... African Green monkey kidney epithelial cells) and Huh-7 cells (human hepatocellular carcinoma cells) were cultured in Dulbecco’s minimal essential medium (DMEM; Life Technologies), supplemented with 10 % fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2019Quote: IMR-90 primary human lung fibroblasts (ATCC #CCL-186) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco #12430-054) supplemented with penicillin and streptomycin (5000 U/mL and 5000 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Macrophages were obtained from the EpCAM− cells after 2 hours of adherence to the tissue culture dish and were cultured with 50 ng/ml human macrophage colony-stimulating factor (M-CSF, #CYT-308, ProSpec) in RPMI-1640 (Gibco) containing 20% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: A total of 10 technical replicates of human neuroblastoma SH-SY5Y cells were cultured in neurobasal media (Gibco 21103-049) supplemented with B-27 Plus (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... In order to generate the construct for inducible ZEB2 expression from the AAVS1 site the coding sequence of the human ZEB2 transcript variant 1 (NM_014795.3) was purchased as an ultimate ORF clone in the pENTRY221 (Thermo Scientific, #IOH53645). The ZEB2 ORF was amplified with primers ZEB2_IndOE_F & _R and tagged with GFP-Flag by Gibson assembly into the pT2-CAG-MCS-2A-Puro plasmid linearized by restriction digestion with EcoRI (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... CPA and CPD cells were cultured in Keratinocyte-SFM supplemented with 2.5 μg prequalified human Epidermal Growth Factor 1-53 (EGF) (Life technologies), 25 mg Bovine Pituitary Extract (BPE ...
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bioRxiv - Cell Biology 2021Quote: ... immortalized human lung fibroblast cells and HCT116 colorectal cancer cells were cultured in a 1:1 mixture of DMEM (Gibco) and Ham’s F10 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... kind gift from Peter Bredenbeek, LUMC, NL) and HuH7 (human hepatoma, JCRB0403) cells were maintained in minimal essential medium (Gibco) supplemented with 10% fetal bovine serum (Integro) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured in BBB induction medium: human endothelial cell serum-free medium (HESFM) (Cat. no. 11111044, Thermo Fisher Scientific) supplemented with 1% human platelet poor derived serum (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with pEGFP-human LA (EGFP-wt LA) and mutants after 3h from release of the nocodazole block using Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s protocol at early passages having 80-85% confluency and were kept in culture for 24 hrs [67] ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney 393T cells were adapted to suspension culture in Gibco Freestyle 293 medium (Thermo Fisher Scientific, Waltham USA) supplemented with 20 U/L penicillin ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney cells adapted to suspension culture (HEK293F) were grown in Free Style 293 Expression Medium (Thermo Fisher Scientific) and harvested at a density of 3 million cells/ml ...
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bioRxiv - Biochemistry 2021Quote: ... falciparum cell lines were cultured in human O+ RBCs (Australian Red Cross) with RPMI 1640 medium with GlutaMAX and HEPES (ThermoFisher), supplemented with 5% v/v human serum (Australian Red Cross) ...
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bioRxiv - Cell Biology 2021Quote: The VEGF-A concentration in the culture medium was analyzed using a VEGF-A Human ProcartaPlex Simplex Kit (Cat. no. EPX01A-10277-901, Invitrogen), specific for the detection of VEGF-A165 ...
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bioRxiv - Biochemistry 2020Quote: ... serum reactivity to the Spike protein was measured using an HRP-conjugated goat anti-human IgG (H+L) (Invitrogen, 31410) at a 1:3000 dilution for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: Surgically-resected human brain metastases and newly diagnosed glioblastomas were collected in neurobasal media A supplemented with 1x B27 (17504-044, Gibco), 1x N-2 (17502-048 ...
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bioRxiv - Cell Biology 2021Quote: ... both human primary myogenic progenitors isolated from female donors (n=2-5 per experiment) and commercialised human primary skeletal muscle progenitors (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... fresh human pancreatic islets preparations were resuspended in CMRL culture medium (cat. 11-530-037, ThermoFisher Scientific, Waltham, MA, USA) supplemented with L-Glutamine 1% (cat.G7513-100ML) ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were washed three times with staining buffer and incubated in 0.01 μg/mL AF 488 secondary anti-human IgG (Invitrogen, A18821) solution for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... endothelial cells were expanded for 2 days by applying endothelial cell medium (EC+/+) based on human endothelial SFM (Life Technologies) supplemented with 1% platelet-poor plasma derived bovine serum (Alfa Aesar) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IPSCs were maintained on human Laminin-521 (BioLamina, Sundbyger, Sweden) coating in essential 8 (E8) medium (DMEM/F12 supplemented with 15 mM Hepes (Gibco/Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: The 2019-nCoV receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen). The 2019-nCoV RBD (residues Arg319-Phe541 ...
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bioRxiv - Cancer Biology 2021Quote: ... SK-N-FI) and NCI-H23 cells (human NSCLC) were cultivated at 37°C with 5 % CO2 in RPMI 1640 medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... of SARS-Cov-2 (Genbank accession number YP_009724390.1) was codon optimised for expression in human cell lines and synthesised by GeneArt Gene Synthesis (Thermo Fisher Scientific). The sequence encoding amino acids 2-1273 were cloned into a shuttle plasmid following InFusion cloning (Clontech) ...
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bioRxiv - Immunology 2020Quote: Cytokines (IL-5 and IL-13) in supernatant of human and murine ILC2 cell cultures were analyzed with ELISA kits from Invitrogen. For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Wells were washed three times with 1xDPBS followed by a one hour incubation with horse radish peroxidase-conjugated goat anti-human total Ig at a dilution of 1:6000 (Invitrogen). Wells were then washed four times with 1xDPBS followed by a 5-15 minute incubation with TMB substrate (SeraCare) ...
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bioRxiv - Microbiology 2020Quote: ... and HRT-18G (ATCC CRL-11663; human colorectal adenocarcinoma) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco Life Technologies) supplemented with 8% heat-inactivated fetal bovine serum (HyClone ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with an expression plasmid encoding human ACE2 - C-terminally fused to the GFP - using Lipofectamine 2000 (Invitrogen). Two days post transfection ...
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bioRxiv - Microbiology 2019Quote: Madin-Darby canine kidney (MDCK) and human embryonic kidney HEK293T (293T) cells were maintained in Gibco’s minimal essential medium with GlutaMax (Life Technologies). Human lung epithelial A549 cells were maintained in Gibco’s F-12 medium (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... the cytokine levels were determined by Luminex xMAP technology using Human Custom ProcartaPlex Assay kit (Thermofisher, Cat PPX-04-MXRWEYU) in accordance with the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... on HEK-293T overexpressing the human ACE2 and maintained in the previously described media containing 1 mg/ml of geneticin (Invitrogen) to obtain TMPRSS2/ACE2 HEK-293T cells.
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bioRxiv - Molecular Biology 2021Quote: ... and PToV Hemagglutinin Esterase non-binding (F271A) (1176bp) fused to human IgG-Fc followed by a 6xHis tag in the Insect expression vector pFastBac1 (ThermoFisher) was kindly provided by Colin Parrish (PToV-HE_S46A-Fc_Hisx6/pFastBac1 ...
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bioRxiv - Microbiology 2021Quote: ... The human hepatocellular carcinoma HepG2 cell line (ATCC HB-8065) and the human kidney adenocarcinoma A-704 cell line (ECACC 93020513) were grown in minimum essential medium (MEM, Gibco) with glutamax supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... the cells were fixed for 10 min at 4°C with 100 μL of the Fixation/Permeabilization solution and then washed and stained in 100 μL of the BD Perm/Wash Buffer containing anti-human polyclonal PPARγ antibody (PA3-821A, Invitrogen) at 1:25 for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: The catalytic domain of the human ST6Gal I (residue range 132-406) was expressed in a BL21(DE3) Escherichia coli strain (Invitrogen), using a polycistronic Ptac vector containing the CyDisCo system (Gąciarz et al ...
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bioRxiv - Immunology 2021Quote: ... 30,000 purified cells were stimulated in round-bottom 96-well plates with human anti-CD3/anti-CD28 T cell expander beads (1 bead/cell; ThermoFisher) in complete media containing 10 U/mL rhIL-2 with or without 10 or 20 uM 6-(4-Chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO ...
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bioRxiv - Immunology 2021Quote: ... Expression vector was transiently transfected into Human Embryonic Kidney (HEK) 293 GnTI-Freestyle cells suspension culture in serum-free media (Invitrogen) using polyethyleneimine (Polysciences) ...
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bioRxiv - Immunology 2021Quote: IL-17a ELISAs were performed using Human IL-17A (homodimer) ELISA Ready-SET-Go!™ Kit (Invitrogen™; eBioscience™), according to the manufacturer’s instructions ...