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Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... were from Proteintech Group Inc (Chicago, IL). pAb against claudin-5 (cat. no. 34-1600) was from Invitrogen and mAb against β-actin (cat ...
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bioRxiv - Biochemistry 2020Quote: ... Terbium labelled cells were detached using 5 ml of cell-dissociation buffer (GIBCO, Carlsbad, USA) and collected using 5 ml of DMEM containing 10% FCS ...
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bioRxiv - Molecular Biology 2020Quote: ... which were boiled for 5 min in Laemmli sample buffer (#NP0007, Invitrogen, Waltham, MA, USA). Protein were separated on 4-15 % Tris-glycine (TG ...
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bioRxiv - Cell Biology 2019Quote: ... for 5 min and separated on a 3-8% tris-acetate gel (ThermoFisher Scientific; EA0375BOX). Following electrophoresis ...
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bioRxiv - Microbiology 2019Quote: ... and cultured (37 °C, 5% CO2) on Petri dishes containing DMEM with 1% HEPES (Gibco), 10% FBS and 20% L cell-conditioned medium (LCM ...
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bioRxiv - Molecular Biology 2019Quote: ... Reaction mixtures of 20 µl were composed from 5 µl SYBR Green (Thermo Fisher Scientific), 0.2 µl of each primer ...
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bioRxiv - Cancer Biology 2019Quote: RNA was extracted from HCT116 and HCT116-Dnmt1Δ3-5 cells using TRIzol™ Reagent (Invitrogen) according to the manufactures protocol and stored at −80°C until required ...
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bioRxiv - Cancer Biology 2019Quote: Transfection of HCT116 and HCT116-Dnmt1Δ3-5 cells was performed using Lipofectamin 3000 Reagent (ThermoFisher) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Primer 2: 5’-GGATGTTCGTCCAGTGAGATTAG-3’) using a 7500 Fast Real-Time PCR System (Applied Biosystems) and accompanying software to analyze qPCR data.
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated for 5 min at 37°C in prewarmed TrypLE Express (Gibco™). TrypLE Express was then diluted 1:10 in prewarmed 1x Dulbecco’s phosphate-buffered saline (DPBS) ...
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bioRxiv - Pathology 2019Quote: ... pH 7.4 plus 10 mM EDTA and 5% molecular grade BSA (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2019Quote: ... the plates were developed with the addition of 5 mg o-phenylenediamine dihydrochloride (Thermo Scientific) and stable peroxide substrate buffer (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... The upper layer was transferred to a 2 mL tube PVDF filter (ThermoFisher, F2520-5) and centrifuged for 5 min at 4,000 × g at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... Washed cells were incubated in blocking solution containing 5% normal horse serum (Invitrogen, cat. # 31874) and 0.1% triton-X (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µl were directly loaded on a native polyacrylamide gel (6% DNA Retardation, Thermo Fisher) and run at 4 °C using 0.5 X TBE buffer for 60 min ...
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bioRxiv - Genetics 2020Quote: ... for 5 min prior to mounting in Prolong Gold antifade mounting medium (ThermoFisher Scientific, USA).
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bioRxiv - Synthetic Biology 2019Quote: ... and MIT_v2.1_SbfInifJ_RV2 5’-AACCTGCAGGGCTAACTAACTAACCACGGACAAAAAACC-3’) and ligated into pCR Blunt II TOPO (Thermo Fisher Scientific). The second half containing nifBQFUSVWZM was amplified with SbfI sites on either end (with oligos MIT_v2.1_SbfInifB_FW 5’-AACCTGCAGGTACTCTAACCCCATCGGCCGTCTTA-3’ ...
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bioRxiv - Neuroscience 2019Quote: ... and in the case of NOCL1 and NOCL2 also 5% fetal calf serum (ThermoFisher Scientific). NGN1 expression was induced by addition of doxycycline (2 μg/ml ...
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bioRxiv - Physiology 2019Quote: RNA was isolated from WT and DKO livers (n=5 per group) using TRIzol (Invitrogen), reverse-transcribed ...
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bioRxiv - Cancer Biology 2019Quote: Microscopy images were obtained after dye incubation and 5 minutes of NucBlue (Molecular Probes R37605) staining ...
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bioRxiv - Biophysics 2019Quote: ... 5% CO2 atmosphere in Dulbecco’s modified Eagle’s medium with 1 g/mL glucose (Gibco 31885023) supplemented with 1% penicillin-streptomycin (Sigma P0781) ...
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bioRxiv - Immunology 2020Quote: PBMCs were incubated overnight (37°C, 5% CO2) in X-vivo 15 media (Fisher Scientific) supplemented with 5% AB Serum (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... and MitoSOX Red mitochondrial superoxide indicator (5 μM, 30 minutes) (Molecular Probes, Invitrogen, Paisley, UK), respectively ...
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bioRxiv - Physiology 2020Quote: ... and MitoSOX Red mitochondrial superoxide indicator (5 μM, 30 minutes) (Molecular Probes, Invitrogen, Paisley, UK), respectively ...
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were mounted with 5 µL of ProLong antifade reagent (Thermo Fisher Scientific, USA), covered with cover glass ...
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bioRxiv - Cell Biology 2019Quote: ... Itgβ1 (TS2/16), SNX6 (Clone D-5; 365965; Santa Cruz Biotechnology, PMP70 (PA1-650; Invitrogen), WASH1 (gifted from D.D ...
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bioRxiv - Developmental Biology 2021Quote: ... at 37°C in a HERAcell 150i or 160i 5% CO2 incubator (Thermo Fisher Scientific). Cells were split 1:4 – 1:10 every 2-3 days when approximately 80% confluent and kept in 6-well dishes or uncoated flasks using Trypsin (0.25%)-EDTA (0.02% ...
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bioRxiv - Developmental Biology 2021Quote: ... at 37°C in a HERAcell 150i or 160i 5% CO2 incubator (Thermo Fisher Scientific). Cells were passaged every 4 days at ∼1 x 105 – 2 x 106 cells/mL in uncoated flasks by pelleting at 150g for 5 minutes and resuspending in fresh media.
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Neuroscience 2021Quote: ... samples were heated for 5 minutes at 95°C in sample buffer (#NP0007, Invitrogen, USA), and then separated on 4-12% Bis-Tris mini gels (#NP0336 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were labeled in 5% BSA/TBST with either Streptavidin-HRP (1:10,000, Thermo Fisher), anti-HA peroxidase conjugated antibodies (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... HFFs were transfected with 5 μg total DNA using a Neon electroporation system (ThermoFisher Scientific) and plated on polyacrylamide gels (traction force microscopy ...
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bioRxiv - Molecular Biology 2021Quote: ... Fluorescence signal was dynamically measured by QuantStudio™ 5 Real-Time PCR System (Thermo Fisher). Background-subtracted signals for each monitoring points were further normalized by subtraction of its initial value to make comparison between different conditions (arbitrary unit ...
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR was performed in a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific) using the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... 106 293T cells were transfected with 5 µg infectious clone using Lipofectamine 2000 (Life Technologies), cells were freeze-thawed after 1 d ...
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bioRxiv - Microbiology 2021Quote: Mouse lung tissue sections were sectioned via cryostat (Thermofisher CryoStar NX70 cryostat, 5 μm/section) and fixed onto glass slides ...
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bioRxiv - Microbiology 2021Quote: ... with 5% HyClone iron-supplemented calf serum (Cytivia, SH30073.03) and 1% penicillin-streptomycin (Invitrogen, 15140122), and were maintained at 37 °C and 5% CO2 ...
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bioRxiv - Genomics 2019Quote: ... Six picomole of siRNA was transfected into 5×104 cells using Lipofectamine RNAiMax (Thermo Fisher) following the reverse transfection procedure in 24-well format ...
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bioRxiv - Biochemistry 2021Quote: ... then fixed with 4% paraformaldehyde containing 5 µg/ml Hoechst 33342 stain (Thermo Fisher, #62249) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 150nM of each primer and 5 μl 2X-Jump Start SYBR Green PCR Mix (Invitrogen) in10 μl total volume ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... An anion-exchange HPLC column (BioBasic AX, 150 x 2.1 mm, 5 µM, Thermo Scientific) was used for the chromatographic separation and a 5500 QTrap (Sciex ...
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bioRxiv - Immunology 2021Quote: C-LP cells pooled from 5-7 mice were sorted into TriZol® (Thermo Fisher) and cDNA was generated by using QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... For experiments using OP9 stromal cells: OP9 cells were labeled with 5 μM CFSE (Invitrogen). Immune complexes containing mono-biotinylated lysozyme and anti-sera generated in mice were formed in vitro ...
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bioRxiv - Cell Biology 2019Quote: ... All cells were grown at 37°C in 5% CO2 and maintained in DMEM (Invitrogen) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2020Quote: ... then re-suspended in 40 μl of 5% mass spectrometry grade acetonitrile (Thermo Fisher Scientific) and 0.5% formic acid ...
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bioRxiv - Plant Biology 2020Quote: ... and 0.05 μl of Platinum Taq DNA Polymerase (5 U/μl, Invitrogen, Carlsbad, CA, USA). Gene expression was evaluated using a modified 2-ΔCT method (Schmittgen and Livak 2008) ...
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bioRxiv - Cancer Biology 2020Quote: ... at 37°Cin a humidified incubator with an atmosphere of 5% CO2 (Thermo fisher scientific). The cells were passaged at a density of 2.0×105 cells in a 25cm2 flask or cryopreserved at a density of 2.0×106/mL.
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bioRxiv - Animal Behavior and Cognition 2019Quote: COS7 cells were maintained at 37°C under 5% CO2 in DMEM media (Life Technologies) supplemented with 10% fetal bovine serum (Atlanta Biologicals) ...
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bioRxiv - Cell Biology 2019Quote: ... 5 µg of RNA from each sample was treated with TURBO™ DNase (ThermoFisher AM2238) followed by clean-up and enrichment of RNA > ∼150 nt using RNA clean and concentrator (Zymo Research #R1013) ...
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bioRxiv - Developmental Biology 2019Quote: For live imaging 3-5 day old flies were dissected in Schneider’s Insect Media (Thermofisher) supplemented with 10% Fetal Bovine Serum (FBS ...