Labshake search
Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations for Heme oxygenase 1 HO 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... were activated with anti-CD3/CD28 beads at a 1:1 ratio (Dynabeads, GIBCO, Cat#113.31D) in a 96-well round bottom plate with 100U/mL rhIL-2 (Peprotech ...
-
bioRxiv - Cell Biology 2020Quote: ... resuspended in 1 ml staining solution (1 µM Sytox green –ThermoFisher S7020, 50 mM Tris pH8.0), sonicated briefly using a microtip sonicator ...
-
bioRxiv - Cell Biology 2020Quote: ... 2.5mM EDTA and 1%(vol/vol) Triton X-100) containing 1:4000 SYBR Green I (Invitrogen) was added to the wells and the plates were incubated overnight at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... plasmids (1 μg of guide RNAs +/- 3 μg donor) were diluted in 1 ml OptiMem (Gibco) and 20 ul PEI (1 mg/ml) ...
-
bioRxiv - Cell Biology 2020Quote: ... Alexa568- (1:1000) and Alexa647-coupled (1:500) antibodies were used (all from Thermo Fisher Scientific). For Western blotting ...
-
bioRxiv - Immunology 2021Quote: ... 1 × 105 activated NKT cells were incubated with 1 mM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Invitrogen) in RPMI complete media for 30 minutes at 37°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... and Adgrg6 KO ATDC5 cells were maintained in DMEM/F-12 (1:1) medium (Gibco, 11330032) supplemented with 5% FBS and 1% penicillin/streptomycin ...
-
bioRxiv - Molecular Biology 2020Quote: ... Gels were stained with 1 mL 1/1,000 SYBR Gold Nucleic Acid Gel Stain (ThermoFisher Scientific) and imaged with the GelDoc™ XR+ system (BioRad).
-
bioRxiv - Evolutionary Biology 2021Quote: ... including the DNase treatment and were precipitated by adding 1:1 LiCl (7.5 M) (Invitrogen AM9480) and incubating at −20°C for 30 minutes followed by centrifugation at full speed at 4°C for 15 minutes and extensive EtOH washes ...
-
bioRxiv - Developmental Biology 2020Quote: ... 594 or 647 fluorophores (1:500 for whole-mount to 1:2000 dilutions for cryosections; Invitrogen).
-
bioRxiv - Developmental Biology 2020Quote: ... embryoid bodies were embedded in Matrigel and transferred to differentiation medium (1:1 DMEM/F12 (Gibco) / Neuralbasal (Gibco ...
-
bioRxiv - Developmental Biology 2021Quote: ... stained with Sytox Green 1:1000 and Phalloidin 555 1:300 in PBS overnight (Molecular Probes). Tunel stained tissue was processed after sectioning using the Click-iT TUNEL Alexa Fluor 488 kit according to manufacturer’s instructions (Invitrogen) ...
-
bioRxiv - Cancer Biology 2022Quote: ... overnight followed by secondary (anti-rabbit ThermoFisher #31466 1:10000, anti-mouse ThermoFisher #31431 1:10000) for one hour ...
-
bioRxiv - Microbiology 2022Quote: ... Goat anti-mouse IgG Alexa Fluor 488 (1:500 dilution in 1% BSA in PBS; Invitrogen) was used as the secondary antibody ...
-
bioRxiv - Molecular Biology 2020Quote: ... DAPI (1:1,000 from 1mg/mL stock) and CellMask Orange or Deep Red (1:200,000, Invitrogen). Images were acquired using the IN Cell 2200 or 6000 automated microscope (GE ...
-
bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
-
bioRxiv - Molecular Biology 2021Quote: ... to which was added 1µL of 1:100 ERCC Spike-In Mix 1 (Invitrogen; Cat #4456740), commonly employed to control for cross-sample variation in library preparation ...
-
bioRxiv - Genomics 2019Quote: ... 1% Pen/Strep (Bio-Concept) and 1% MEM-NEAA (MEM non-essential amino acids, ThermoFisher Scientific). All cell lines were confirmed negative for mycoplasma infection using the PCR-based Universal Mycoplasma Detection kit (American Type Culture Collection ...
-
bioRxiv - Microbiology 2020Quote: ... 20 μl RNase A/T1 mix (2 μg μl−1 / 5 U μl−1, Thermo Scientific) was added to 100 μl lysate (OD260 ~ 150 ...
-
bioRxiv - Molecular Biology 2019Quote: ... and 100 U ml-1 penicillin and 100 µg ml-1 streptomycin (Pen/Strep; Wisent/Gibco). HeLa Flag-Cas9 and 293T cells were cultured in DMEM supplemented with 10% FBS and Pen/Strep ...
-
bioRxiv - Developmental Biology 2019Quote: ... Undigested and digested products were resolved in a 3% Metaphor 1:1 (lonza)/agarose gel (Invitrogen) gel ...
-
bioRxiv - Cancer Biology 2019Quote: ... 1×106 cells were transfected with 1:2 ratio of plasmid and Lipofectamine 3000 (Thermo Scientific). The protein or mRNA were isolated after 36-48 hours of transfection ...
-
bioRxiv - Immunology 2020Quote: ... the cell suspension was resuspended in pMCPECs media containing DMEM/F12 1:1 (Gibco, Paisley, UK), FBS 10% (Gibco ...
-
bioRxiv - Genomics 2019Quote: ... Nuclei concentration and integrity were determined using Ethidium Homodimer-1 (EthD-1) (#E1169, Thermo Fisher Scientific). Finally ...
-
bioRxiv - Bioengineering 2019Quote: ... 1 μL M-MLV Reverse Transcriptase (200 U μL−1, Invitrogen, 28025-013, Breda, the Netherlands), and RNAse-free ultra-pure water (ddH2O ...
-
bioRxiv - Immunology 2019Quote: ... 1% (w/v) L-glutamine and 1 % (w/v) penicillin/streptomycin (Gibco; ThermoFisher Scientific, VIC, Australia).
-
bioRxiv - Microbiology 2019Quote: ... coupled with an EASY nLC 1 000 or 1 200 chromatography system (Thermo Fisher Scientific, Bremen). Sample was loaded on an in-house packed 50 cm nano-HPLC column (75 μm inner diameter ...
-
bioRxiv - Microbiology 2019Quote: ... followed by Alexa Fluor® 594 goat anti-rabbit (1:250, 1 h) (Invitrogen, A-11012). Detection of basal bodies (YL1/2) ...
-
bioRxiv - Developmental Biology 2019Quote: ... A 0.3M sucrose solution was mixed 1:1 with DiD vibrant dye solution (V22887 Thermo Fisher) and injected into the neural tubes of embryos at HH9 for neural crest labeling.
-
bioRxiv - Microbiology 2019Quote: ... Resulting reactions were incubated for 1 hour at 37°C with 1 µL TURBO DNase (Invitrogen) after which 21 µL 2X formaldehyde loading dye (Thermo Scientific ...
-
bioRxiv - Bioengineering 2020Quote: ... consisting of a 1:1 mix of DMEM/F12 with GlutaMAX and Neurobasal media (Gibco, #21103049) supplemented with 1:200 GlutaMAX (Gibco ...
-
bioRxiv - Biochemistry 2020Quote: ... Saccharomyces cerevisiae strain InvSc1 (MATa adc2-1 his3-11,15 leu2-3,112 trp1-1 ura3-52; Invitrogen) was grown in synthetic dextrose medium (5 mL ...
-
bioRxiv - Neuroscience 2020Quote: ... Bassoon (1:400, SySy) followed by incubation with secondary antibodies (1:400, Alexa Fluor-conjugated, Invitrogen), counterstained with DAPI and mounted in Fluorsave (Calbiochem) ...
-
bioRxiv - Neuroscience 2020Quote: ... with additional 1 mM CaCl2 and 1 mM MgSO4 (all from Thermo Fisher Scientific; Waltham, MA). Medium was refreshed every 3–4 days ...
-
bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight with antibodies directed to ZO-1 (61-7300, ThermoFisher Scientific, 1/100), E-cadherin (ab76055 ...
-
bioRxiv - Neuroscience 2020Quote: ... 2µl of diluted 1:1000 diluted ERCC Spike-in Control Mix 1 (Ambion by Life Technologies) was added to 100ng of each RNA sample prior to library construction ...
-
bioRxiv - Neuroscience 2020Quote: ... 2µl of diluted 1:1000 diluted ERCC Spike-in Control Mix 1 (Ambion by Life Technologies) was added to 100ng of each RNA sample prior to library construction ...
-
bioRxiv - Biochemistry 2021Quote: ... goat anti-rabbit HRP conjugated antibody (WB 1:2000 or 1:5000, Thermo Fisher Scientific, 31460), goat anti-mouse HRP conjugated antibody (WB 1:5000 ...
-
bioRxiv - Biochemistry 2020Quote: ... Membranes were then incubated with Avidin-HRP (1:1000 in blocking buffer, ThermoFisher, 1 hr, r.t.) followed by three washes with PBST ...
-
bioRxiv - Neuroscience 2021Quote: ... The Alexa 488 goat anti-rabbit secondary antibody (1:1500 in 1% NGS PBST, Life Technologies) was applied for one hr at RT℃ ...
-
bioRxiv - Neuroscience 2021Quote: ... were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (1:1 v/v, Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... made of 5:1:1 ratio of H2O : H2O2 (50 wt. % in H2O, stabilized, Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2020Quote: ... at which point 1×106 cells were collected and fixed in 1% FA (Thermo Fisher 28908) in PBS for 10 minutes at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 h with secondary antibodies (anti-Mouse IgG 488, 1:1000 Invitrogen, #A11029 ...
-
bioRxiv - Plant Biology 2021Quote: ... The second group was treated with proteinase (1 ng μL-1 Proteinase K Thermo Fisher Scientific) and RNase (0.4 ng μl-1 RNase A ...
-
bioRxiv - Biochemistry 2020Quote: ... The sample was then mixed 1:1 with NuPAGE LDS sample buffer (Thermo Scientific, Cat# NP0007) and boiled at 95 °C for 10 min ...
-
bioRxiv - Cell Biology 2021Quote: ... the 2i ESCs were maintained in a 1:1 mix of DMEM/F12 (Gibco, 11302-33) and Neurobasal medium containing N2 supplements ...
-
bioRxiv - Plant Biology 2020Quote: ... and limited cell lysis was detected by staining with 1:1 trypan blue (Thermo Scientific 12250061). Cells were loaded into a microfluidic device (10X Genomics ...
-
bioRxiv - Systems Biology 2020Quote: ... rehydrated in 1 mL rehydration buffer containing 0.01% PBS/BSA and 1:100 Superasein (Thermo Fisher), and resuspended in 400 µL rehydration buffer followed by passing through a 40 µm cell strainer ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were incubated with Alexa-labeled secondary antibodies for 1 h (1:500, Molecular Probes, USA). Sections were then washed in PBS and mounted in Fluoromount containing DAPI (Calbiochem ...