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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Whole genome transcript analysis was performed on 200 ng of total RNA using the Affymetrix Human Gene ST version 2.0 Array (Affymetrix). Genome-wide DNA methylation was profiled using the Illumina EPIC platform (Illumina ...
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bioRxiv - Physiology 2020Quote: Cryo-preserved primary human hepatocytes from single donors (PHH; BD Gentest, Tewksbury, MA; Lonza, Walkersville, MD; Thermo Fisher Scientific) and the human HCC cell line HuH7 (ATCC ...
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bioRxiv - Immunology 2019Quote: ... The plate was incubated with detection antibody (mouse anti-human IgG1 Fc conjugated to horseradish peroxidase clone HP6069; ThermoFisher) for 2 hours at room temperature ...
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bioRxiv - Immunology 2019Quote: A cDNA encoding wild-type (WT) human TRIM22 (a gift form Professor Juergen Hass, University of Edinburgh) was inserted into pcDNA.3.1 (Invitrogen) in frame with a 5’ Myc-tag oligo to generate a pcDNA.Myc.TRIM22 ...
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bioRxiv - Cell Biology 2020Quote: ... For RHBDL4 and OST4 depletion 50 pmol (RHBDL4) or 25 pmol (OST4) ON-TARGETplus SMARTpool human siRNA (Dharmacon) and 2.5 μl RNAimax (Invitrogen) transfection reagent per well of a 12-well plate were used ...
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bioRxiv - Cell Biology 2019Quote: ... Human telomerase reverse transcriptase immortalized retinal pigment epithelial cells (TERT-RPE1, ATCC CRL-4000) were cultured in DMEM:F12 (Gibco) supplemented with 10% FBS and 1% penicillin and streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: Copy number analysis was derived from profiling of total DNA on the Affymetrix genome-wide human SNP Array 6.0 chip using instrumentation and protocols from Affymetrix. The raw CEL files were returned and the QC steps were performed using Affymetrix Genotyping Console ...
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bioRxiv - Genomics 2019Quote: Human islets were washed once in cold PBS and dissociated into single cells by enzymatic digestion with Accumax (Invitrogen), followed by digestion using freshly prepared Dispase (Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... with 12.5 pmol of either non-targeting negative control siRNA showing no homology to the human transcriptome (#4457287, Ambion), or two different siRNAs targeting OAS1 (siRNA-1 or −2 ...
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bioRxiv - Immunology 2020Quote: ... T cells were polyclonally expanded from freshly isolated PBMCs using Dynabeads human T-activator CD3/CD28 beads (Life Technologies) as previously described (Loef et al ...
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bioRxiv - Molecular Biology 2020Quote: Human HCT116 cells (ATCC) were cultured at 37°C under 5% CO2 in McCoy’s 5A media (Thermo Fisher Scientific) with 1x penicillin/streptomycin and 10% FBS (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: Fresh-frozen human postmortem brain tissues were sectioned at 14-16 μm onto Superfrost Plus glass slides (Fisher Scientific). Sections were dried for 20 minutes at −20°C and then vacuum sealed and stored at −80°C until use ...
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bioRxiv - Cancer Biology 2021Quote: Non-targeting siRNA and siRNA against mouse and human LIG3 were transfected into the cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Human glioblastoma cell line U-251MG was cultured in Dulbecco’s Modified Eagle Medium (DMEM-HG) (11995-065; Gibco, USA) and supplemented with 10% FBS (RM10409 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays: human GAPDH control ID: Hs99999905_m1(ThermoFisher), zebrafish abcb4 ID ...
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bioRxiv - Cancer Biology 2020Quote: ... wells were filled with 250μl of human liver organoid isolation medium consisting Ad+++ supplemented of 1X B27 supplement without retinoic acid (Gibco), 1X N2 supplement (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... and resuspended in complete medium in the absence of human serum or Pf-iRBCs schizonts were isolated in RPMI 1640 medium supplemented with 0.25% Albumax (GIBCO, Invitrogen) and 7.4% Sodium Bicarbonate (GIBCO ...
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: Microarray analyses were conducted using the human Clariom D Affymetrix platform following the manufacturer’s instructions (902915, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... the coding sequence of the current human textitTRBP reference (RefSeq NM_134323) was amplified and subcloned into the pcDNA3-cMyc vector (Invitrogen) at the BamHI and XhoI sites ...
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bioRxiv - Cancer Biology 2020Quote: CAFs were isolated from human colon carcinoma resection specimens following 2.4U/ml dispase + 100U/ml collagenase type II (both from Invitrogen); disaggregation of tumour fragments and digestion for 2h t 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and hybridized to the Affymetrix Human Gene 1.0 ST Arrays using the Gene Chip WT Terminal Labeling and Hybridization Kit (Affymetrix). GeneChip fluidics station 450 was used for processing of the arrays and fluorescent signals were detected with the GeneChip scanner 3000-7 G ...
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bioRxiv - Bioengineering 2021Quote: ... media was changed to the following formulation of EC medium: human endothelial serum-free medium (hESFM, Thermo Fisher 1111044) supplemented with 1% platelet-poor plasma-derived serum (PDS ...
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bioRxiv - Immunology 2020Quote: ... Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher, #A1049101) supplemented with 10% FBS ...
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bioRxiv - Genomics 2019Quote: ... Activation was performed by addition of Dynabeads Human T-Activator CD3/CD28 (25 ul/0.5 million cells; Life Technologies), along with IL-2 (30 U/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... H1299 (human non-small cell lung carcinoma, ATCC CRL-5803) and HMGA2 KO cells were cultured in RPMI (GIBCO) supplemented with 10% FBS (Gibco ...
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bioRxiv - Genomics 2021Quote: 40-80 ng of each probe (labeled with biotin or Dig) and 1µg of unlabeled human COT1-DNA (Invitrogen) were dissolved in 4µl of hybridization buffer (10% dextran sulfate ...
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bioRxiv - Genomics 2021Quote: Human chronic myelogenous leukemia K562 cells (ATCC CCL-243) were cultured in RPMI 1640 Medium with GlutaMAX (Gibco 61870127), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Genetics 2020Quote: ... HeLa and Human foreskin fibroblast (HFF) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS (Gibco). N2a cells were maintained in 45% DMEM and 45% Opti-MEM supplemented with 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: The AGS human gastric epithelial cell line (ATCC CRL-1739) was maintained in DMEM/F-12 (Thermo Fisher Scientific) containing 10% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... and Human pancreatic epithelial cells (hTERT-HPNE, ATCC, CRL-4023) were cultured in pyruvate-free DMEM (Gibco, 11965-092) supplemented with 10% FBS and P/S ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA from native pig and human lung tissue (fresh snap-frozen) was isolated using the PureLink Mini Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Synthetic genes encoding for human merlin FERM (22-312) and radixin FERM (5-295) domains were commercially synthesized (ThermoFisher) and cloned into pETM33 using NcoI and EcoRI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: ... The peptide–mRNA fusions were then incubated with human and yeast DUBm-immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Human ASGR1 and its mutants (Q240A/W244A and Q240A/W244A/E253A) and human ASGR2 were sub-cloned into pcDNA 3.1 vector (Invitrogen) containing a C-terminal Flag tag (ASGR1 ...
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bioRxiv - Synthetic Biology 2021Quote: Flp-In™ T-REx™ Human Embryonic Kidney (HEK) 293 cells were purchased from Thermo Scientific (Catlog# R78007). Cells were cultured in a humidity-controlled incubator under standard culture conditions (37°C with 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: A human episomal iPSC line derived from CD34+ cord blood was used in this study (A18945, ThermoFisher Scientific; [58]). This cell line has already been validated in its ability to generate light-sensitive 3D retinal organoids ...
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bioRxiv - Immunology 2021Quote: ... This was followed by addition of alkaline phosphatase-conjugated goat anti-human F(ab’)2 antibody (Thermo Fisher Scientific) Inc. ...
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bioRxiv - Immunology 2021Quote: Cells from the human cancer cell line Colo205 were cultivated in complete RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 2 mM L-glutamine ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 human IECs (ATCC #HTB-37) were grown in Dulbecco’s Modified Eagle Medium (DMEM; #11965, Gibco-ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 human IECs (ATCC #HTB-37) were grown in Dulbecco’s Modified Eagle Medium (DMEM; #11965, Gibco-ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: The viral-integration-free human iPSC line generated using cord blood-derived from CD34+ progenitors was obtained from Gibco™/Thermo Scientific (Cat ...
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bioRxiv - Physiology 2021Quote: ... slgA concentrations in saliva were measured using the ready-set-go ELISA system for human IgA (Affymetrix: eBioscience, USA) with a sample dilution of 1:7500 at the University Hospital RWTH Aachen (SI ...
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bioRxiv - Cancer Biology 2020Quote: Human T-ALL cell lines (ARR, DU.528, HSB2, and CCRF-CEM) were cultured in RPMI 1640 medium (Gibco), supplemented with 10% heat inactivated Foetal bovine serum (HI-FBS ...
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bioRxiv - Evolutionary Biology 2021Quote: Human ESCs (WA01, also called H1, from WiCell Research Institute) were cultured with StemFlex Medium (Gibco, Cat. No. A3349401) in a feeder cell-free condition ...
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bioRxiv - Cancer Biology 2021Quote: Human breast cancer cell line MDA-MB-468 cells were cultured in Leibovitz L-15 medium (Gibco #21083-027) supplemented with 10% heat-inactivated FBS and 1xpenicillin/streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... was introduced to primary human umbilical vein endothelial cells (HUVEC) using the Neon® transfection system (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: Human telomerase-immortalised retinal pigment epithelial cells (hTERT-RPE1, ATCC) were grown in DMEM-F12 (Life Technologies, Paisley, UK) supplemented with 10% decomplemented FCS (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... Human alpha thrombin (Nordic Diagnostica AB, Billdal, Sweden) was resuspended in OptiMEM reduced serum medium (RSM) (Thermo Fisher Scientific) to 1 U/μl and stored at −20 °C ...
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bioRxiv - Biochemistry 2020Quote: The human colon cancer cell line HCT 116 was grown in McCoy’s 5A medium with glutamax (GIBCO, 36600–021) to which 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells; ATCC CCL-244) were cultured in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...