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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: Purified cell-free synthesized lysozymes were assayed with the EnzChek® Lysozyme Assay Kit (Thermo Fisher Scientific, Waltham, MA). The assay was performed according to protocol with minimal modifications ...
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bioRxiv - Microbiology 2020Quote: ... The DNA concentration of the input DNA was determined using the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... 10 ng of clarified cell lysate (calculated using Pierce Microplate BCA Protein Assay Kit - Reducing Agent Compatible, ThermoFisher Scientific) was incubated with 1 ng of digoxigenin-labelled m67 probe (double-stranded ...
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bioRxiv - Microbiology 2020Quote: We performed a multiplex RT-qPCR assay using the TaqPath RT-PCR COVID-19 kit (Thermo Fisher CN A47814) together with the TaqPath 1-step master mix – No ROX (Thermo Fisher CN A28523) ...
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bioRxiv - Microbiology 2020Quote: ... the final concentration of all the libraries were measured using the Qubit® dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... following the manufacturer’s instructions and quantified on the Qubit 3.0 fluorometer using the DNA high sensitivity assay kit (Thermo-Fisher Scientific). Genomes of the bacterial isolates were sequenced using Illumina MiSeq and PacBio platforms at either Apical Scientific (Selangor ...
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bioRxiv - Neuroscience 2020Quote: ... The protein levels in the supernatants were determined using the BCA Protein Assay Kit (Pierce/Thermo Scientific, Rockford, IL). To detect BDNF ...
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bioRxiv - Neuroscience 2020Quote: ... The total protein concentration was measured with bicinchoninic acid protein assay (Pierce™ Micro BCA Kit, ThermoFisher Scientific, USA). Human brain-derived Aβo was characterized by Western blot analysis and atomic force microscopy (details in Additional file 1 ...
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bioRxiv - Neuroscience 2020Quote: RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). Sequencing libraries were generated from a total amount of 3 μg RNA per sample using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was saved and protein was quantified using a Pierce BCA protein assay kit (ThermoFisher, catalog number 23225). 100 ug of protein was used per sample ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was saved and protein was quantified using a Pierce BCA protein assay kit (ThermoFisher, catalog number 23225). 30 ug of protein was used per sample ...
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bioRxiv - Physiology 2020Quote: ... Explant viability was assessed by measuring lactate dehydrogenase (LDH) release using the Pierce LDH Cytotoxicity Assay Kit (Thermo Scientific) follow manufacturer’s instructions and tissue was either snap-frozen for RNA and protein extractions or fixed in 4% w/v PFA and paraffin embedded for downstream analyses.
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bioRxiv - Microbiology 2021Quote: ... The Invitrogen Qubit 3.0 Fluorometer and the Qubit dsDNA BR Protein Assay kit (ThermoFisher Scientific Inc., Waltham, MA, USA) were used to measure DNA concentrations ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein content in tissue homogenates and EV suspensions were determined by Pierce™ BCA assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... DNA quality and concentration were evaluated using a 0.8% agarose gel and the Quant-iT PicoGreen dsDNA assay kit (Thermofisher). Subsequent to the quality check ...
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bioRxiv - Microbiology 2020Quote: ... The total RNA input was normalized to 15 μg using Qubit™ BR RNA Assay Kit (Invitrogen™, USA) for each respective strain ...
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bioRxiv - Microbiology 2020Quote: ... Each sample was normalized to the same starting amount of cDNA using Qubit™ BR DNA Assay Kit (Invitrogen). cDNA purity was measured using the NanoDrop ND-1000 spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: ... The total protein content in the infected cells was determined using a BCA protein assay kit (Thermo Scientific, USA) with bovine serum albumin as standard ...
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bioRxiv - Microbiology 2020Quote: ... Purified soluble recombinant proteins were tested for bacterial endotoxins using the Limulus Amebocyte Lysate (LAL) assay kit (Invitrogen, USA).
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bioRxiv - Biochemistry 2020Quote: ... and PrpE were purified as decribed.39,57 Protein concentrations were determined with the BCA Protein Assay Kit (Thermo Scientific). Samples were stored as aliquots at −80 °C until further use.
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bioRxiv - Cancer Biology 2020Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was performed using the APO-BrdU TUNEL Assay Kit (Thermo Fisher), according to the manufacturer’s descriptions ...
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bioRxiv - Cancer Biology 2021Quote: ... and protease inhibitor cocktail) and the concentration was measured by Pierce TM BCA Protein Assay Kit (Thermo-Fisher Scientific) for normalization ...
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bioRxiv - Cell Biology 2021Quote: ... The supernant was collected and the protein concentration determined with the Micro BCATM Protein Assay Kit (Thermo Fisher Scientific). 80 µg protein were loaded into a 10 % SDS gel and stained with anti-Tau (clone Tau46 ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentration was measured using InvitrogenTM Qubit® RNA Assay Kit in Qubit® 2.0 Fluorometer (Thermo Fisher Scientific). RNA integrity was assessed using the Agilent RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent ...
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bioRxiv - Cell Biology 2021Quote: Living and dead neurons and Nuro 2a cells were evaluated using a LIVE/DEAD Viability Assay kit (Life Technologies). In brief ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected and total protein concentration was determined using Micro BCA™ protein assay kit (Thermo Scientific). Protein samples were then reduced with 10 mM DTT for 1 h at 60 °C and subsequently alkylated using a 20 mM IAA in dark at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA concentration was quantified using Qubit™ ds DNA High-Sensitive Assay kit (Thermo Fisher Scientific, Waltham, MA, USA) on the Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Libraries were assessed for mass using the Qubit 1X dsDNA HS Assay kit (catalog # Q33231; Invitrogen, Waltham, MA, USA), for fragment size using the High Sensitivity NGS Fragment Analysis kit on a Fragment Analyzer capillary electrophoresis system (catalog # DNF-474-0500 ...
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bioRxiv - Pathology 2022Quote: ... Per initial RNA quantification (Invitrogen Qubit 2.0 Fluorometer and RNA High Sensitivity Assay Kit, Thermo Fisher Scientific, Waltham, MA) and Fragment Analyzer quality control subsets (Fragment Analyzer Automated CE System and High Sensitivity RNA Assay Kit ...
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bioRxiv - Genetics 2022Quote: ... The concentration of each RNA-seq library was determined using Qubit dsDNA BR Assay Kit (Invitrogen, catalog no. Q32853). Approximately 96 RNA-seq libraries were pooled and quantified with the 2100 Bioanalyzer (Agilent ...
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bioRxiv - Bioengineering 2022Quote: ... A subsequent EdU Assay for proliferation was performed using Click-iT EdU Cell Proliferation Kit for Imaging (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... Cleaned products were measured for concentration with a QuBit dsDNA High Sensitivity Assay Kit on a Qubit 2.0 Flurometer (Invitrogen) and run on a QIAxcel Advanced System (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... intracellular OPP was labelled according to the manufacturer’s instructions in the the Invitrogen Click-iT Plus OPP protein synthesis assay kit (C10456, Invitrogen). Analysis was conducted as described by Arguello et al.
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bioRxiv - Genetics 2022Quote: ... and the concentration was measured using a Qubit dsDNA HS assay kit (Qubit 3, Thermo Fisher Scientific Inc., USA). Genomic DNA was shared to a size of approximately 300-400 bp by a Covaris Focused-ultrasonicator M220 (Covaris ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration of the lysates was quantified using a BCA Protein Assay Kit (Thermo Scientific Pierce Biotechnology, USA) and Benchmark Plus Microplate Spectrophotometer (Bio-Rad ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... The supernatants were collected and quantified for soluble Aβ40 and Aβ42 using enzyme-linked immunosorbent assay (ELISA) kits (Invitrogen, US) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... and the protein concentration was measured with a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Waltham, MA) to normalize the insulin level ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected and protein concentration was determined using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Scientific). Equal concentrations of protein extract were loaded into 4%–12% NuPAGE BisTris gels (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Protein quantification of the collected exosomes was performed using BCA method (Pierce™ BCA Protein Assay kit, ThermoFisher Scientific) and equal amount of exosomes were collected for downstream applications ...
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bioRxiv - Plant Biology 2021Quote: ... RNA concentration was measured using the broad-range Quant-iT RNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). RNA (500 ng ...
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bioRxiv - Plant Biology 2021Quote: ... RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). And then ...
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bioRxiv - Immunology 2020Quote: ... The total protein concentration in each cell lysate was measured using Pierce BCA Protein Assay Kit (Thermofisher, Waltham, MA). The cell lysates containing approximately 20 µg of protein were resolved on Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen ...
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bioRxiv - Pathology 2021Quote: ... The protein concentrations of homogenized tissues were determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, Cat# 23225). The homogenized tissues were then incubated with CM-H2DCFDA probe at 37 °C for 20 min with protection from light ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The protein concentration of cleared samples was determined with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, #23225) with measurements conducted on a GloMax-Multi Plate Reader (Promega ...
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bioRxiv - Microbiology 2021Quote: Purified ThsA protein was freshly prepared and protein concentration was quantified using Qubit Protein Assay Kit (ThermoFisher cat # Q33212). Protein was diluted in wash buffer to a concentration of 147 ng/μl ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was recovered by centrifuge and quantitated by Pierce™ BCA Protein Assay Kit (23227, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Protein lysates were subjected to SDS-PAGE and Western blot.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein concentration was measured using the Pierce™ BCA® Protein Assay Kits (Thermo Fisher Scientific Inc., cat.no. 23227). CD63 antibody (1:500 Thermo Fisher Scientific Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... Library quantity and quality were assessed with Qubit 4 Fluorometer with the 1X dsDNA HS Assay Kit (Invitrogen Q33231) and on a Bioanalyzer 2100 System with a High Sensitivity DNA chip ...
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bioRxiv - Neuroscience 2021Quote: We performed fluorescence in situ hybridization (FISH)/immuno-FISH using the QuantiGene®ViewRNA ISH Cell Assay Kit (Affymetrix) with few modifications from the manual ...