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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for 6 THIOPHEN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... wells were prepared with ice cold HBSS containing 6 µM FM1-43FX (Thermo Fisher Scientific Cat# F35355) with or without tubocurarine ...
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bioRxiv - Microbiology 2023Quote: ... Protein-nucleic acid mixtures were then loaded into either 6% or 10% Novex TBE gels (Thermo Fisher). Gels were pre-run for at least 30 min at 100V in 0.5x TBE buffer before sample loading ...
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bioRxiv - Synthetic Biology 2023Quote: Protein thermal stability was measured by differential scanning fluorimetry using a QuantStudio Pro 6/7 (Applied Biosystems). Protein was brought to a concentration of 10 uM with 5x Sypro Orange dye in a final volume of 20 µL in 20 mM NaPi ...
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bioRxiv - Molecular Biology 2023Quote: ... or electroporated into Hepa1-6 cells using a Neon Transfection System 10 µl kit (ThermoFisher MPK 1096) with the following electroporation parameters ...
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bioRxiv - Neuroscience 2023Quote: ... 6 and 8 weeks of maturation were loaded with 2.5 µM Fluo-3-AM (Molecular Probes, #F1242) dissolved in differentiation medium for 30 min at 37 °C and at 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were cultured in a 6-well plate coated with 0.5 mg Matrigel™ (354230, Fisher Scientific) in complete mTeSR plus media (100-0276 ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed using a QuantStudio 6 Pro Real-Time PCR machine (Thermo Fisher Scientific, Waltham, MA). The following primers (5’-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... The transfection mixtures were replaced at 6 h post-transfection by 293 SFM II expression medium (Gibco), supplemented with sodium bicarbonate (3.7 g/L) ...
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bioRxiv - Biophysics 2024Quote: ... and the entire 10 μl RNA–protein mixture was then loaded onto DNA Retardation Gels (6%) (Invitrogen) with 2.5 μl Novex™ Hi-Density TBE Sample Buffer (5X ...
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bioRxiv - Molecular Biology 2023Quote: ... The melt curve step was carried out in a QuantStudio 6 Real-Time PCR system (Applied Biosystems). The PCR process involved UDG (Uracil-DNA Glycosylase ...
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bioRxiv - Neuroscience 2023Quote: The iPSCs and ESCs were maintained on 6-well plates coated with Geltrex (#A1413302, Thermo Fisher Scientific). For the initial 24 hours following seeding ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcript levels were measured with PowerUp SYBR Green Master Mix in QuantStudio 6 Flex System (Applied Biosystems). Initial activation was performed at 95°C for 20 s ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR analysis was conducted on a QuantStudio 6 Flex Real-Time PCR System (4485697; Thermo Fisher Scientific) with Luna Universal qPCR Master Mix (M3003X ...
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bioRxiv - Cancer Biology 2023Quote: ... Amplification was carried out in a QuantStudio™ 6 Flex Real-Time PCR System (#4485691, Applied Biosystems) under the following conditions ...
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bioRxiv - Bioengineering 2023Quote: ... Bulk GBM cell lines (GBM-43, GBM-6) were harvested using 0.25% Trypsin-EDTA (Thermo Fisher Scientific) and passaged less than 30 times.
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bioRxiv - Cell Biology 2023Quote: ... After 6 hours the medium containing transfection reagents was replaced by FluoroBrite DMEM medium (Thermo Fisher, A1896701), supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... and 2mM DTT) for 30 min at 4 °C and analyzed on 6% TBE gels (Life Technologies) at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was dissolved in 50 μL of 6 M guanidine hydrochloride (GuHCL, Thermo Scientific™, AAJ6078622) and placed on the shaker for 20 min at 1000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR analysis was conducted on a QuantStudio 6 Flex Real-Time PCR System (4485697; Thermo Fisher Scientific) with Luna Universal qPCR Master Mix (M3003X ...
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bioRxiv - Neuroscience 2023Quote: ... day 6 neural organoids were transferred to neural medium containing Neurobasal-A medium (Thermo Fisher Scientific, 10888022), B-27 supplement minus vitamin A (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Each sample consisting of 6 lumbar NP tissues was pooled and stored in RNAlater® Reagent (Invitrogen) at 80°C until isolation ...
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bioRxiv - Immunology 2023Quote: ... 6 to 8 samples were pooled and loaded on the Ion 530™ Chip (Thermo Fisher Scientific) using the Ion Chef™ Instrument (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Mixtures were subsequently fractionated by electrophoresis in precast 6% polyacrylamide gels (Novex TBE Gels; Invitrogen, Waltham, MA) buffered with 0.5X TBE (45 mM Tris borate ...
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bioRxiv - Immunology 2024Quote: ... T cells from C57BL/6 mice were stimulated with coated anti-CD3 (Thermo Fisher, #16-0032-85) (10 μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... All the qRT-PCR probes (mouse IL-6, IL-1β and CCL2) were purchased from Applied Biosystems. The relative RNA expression levels were normalized to the expression of mouse HPRT (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded onto 6-well plates forward transfected with Lipofectamine™ RNAiMAX transfection reagent (Invitrogen, # 13778150) according to manufacturer′s instructions using 30 pmol of siRNA for 72h at 37°C before harvesting ...
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bioRxiv - Bioengineering 2024Quote: ... 6-well cultures were transiently transfected with 7.32 ug of plasmid per well using Lipofectamine 3000 (ThermoFisher). 24 hrs after transfection ...
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bioRxiv - Biophysics 2024Quote: ... pH 8.0) from acetone powder and centrifuged in a Fiberlite F14 6×250 LE rotor (ThermoFisher Scientific) at 10,000 rpm for 30 min at 4 ºC ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were then treated with 4-6 units of Turbo DNase (Thermo Fisher Scientific, cat# AM2238) at 37 °C for 30 min to remove the bulk of plasmid DNA contamination ...
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bioRxiv - Cell Biology 2024Quote: ... Other primary antibodies used for immunofluorescence were mouse anti-acetylated tubulin (clone 6-11B, 32270, Thermo Fisher) at 1:10000 ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were cultured in 6-well plates precoated with 120-180 µg/mL Geltrex (Fisher Scientific, A1413302) in Gibco™ Essential 8™ medium (E8 ...
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bioRxiv - Molecular Biology 2024Quote: 2.5-3.0 × 105 cells were seeded in 6-well dish and reverse transfected using Lipofectamine RNAiMAX (Invitrogen) and 60 pmol siRNA following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... was purchased and prepared according to the instructions with 6% (v/v) defibrinated sheep blood (Thermo Scientific) added ...
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bioRxiv - Cancer Biology 2024Quote: ... before they are moved back to ultra-low attachment 6 well plate (Fisher Scientific 07-200-601) with corresponding culture media ...
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bioRxiv - Microbiology 2024Quote: RT was performed on 6 µl of eluted RNA using MMLV reverse transcriptase (Invitrogen, Carlsbad, CA, USA) with dT17 primer (for polyadenylated transcripts) ...
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bioRxiv - Immunology 2024Quote: ... 6-well culture plates) for 8 days at 37°C and 5% CO2 in RPMI1640 (Life technologies) supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... IL-6 was analyzed from blood serums by sandwich enzyme-linked immunosorbent assay (ELISA; Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... and then blocked for 2 h with PBS-Tween (PBST) containing 2% (v/v) goat serum (Gibco, MD, USA). Three-fold serial dilutions of serum (starting at 1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... was cultured at 2-8×105 cells/ml in Fischer’s medium with 20% horse serum and 2 mM L-glutamine (all Gibco).
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bioRxiv - Molecular Biology 2020Quote: ... proteins were dialysed in PBS for 2 x 2 hours using Slide-A-Lyzer™ Dialysis Cassettes (Thermo Fisher). Protein aliquots were frozen and stored at −80°C until use.
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bioRxiv - Immunology 2022Quote: Purified IL-2 expanded MAIT cells (MAITIL-2) were stimulated for 18 hours with anti-CD3/CD28 Dynabeads (Thermofisher), IL-12 (50ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fura-2-acetoxymethyl ester (Fura-2-AM) and Pluronic F-127 were purchased from Molecular Probes (Eugene, OR, USA) and dissolved in dimethylsulfoxide (DMSO) ...
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bioRxiv - Bioengineering 2019Quote: ... The samples were subsequently transferred to iBlot 2 Nitrocellulose membrane using iBlot 2 Dry Blotting System (Thermo Fisher Scientific) for western blotting ...
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bioRxiv - Cell Biology 2021Quote: 2 mg of 0-2 hr embryos were harvested and RNA was extracted with TRIzol Reagent (#15596026, ThermoFisher Scientific). The PAT test was conducted following the manufacturer’s protocol (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... SALK_015201 and irr-2 genomic DNA using gene specific primers (Supplemental Table 2) with Phusion polymerase (Thermo Fisher Scientific) and cleaned up using the Gene-JET PCR Purification Kit from Thermofisher (catalog number K0701) ...
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bioRxiv - Microbiology 2021Quote: ... 3×105 HEK293T cells were transfected with a 2:1:2 mass ratio of psPAX.2:pMD2.G:pLentiCMV/TO PuroDEST pUL21 (815 ng total DNA) using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... embryos were collected and brains were removed in ice-cooled Ca+2/Mg+2-free Hank’s balanced salt solution (Gibco) with 10mM HEPES (Thermofisher) ...
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bioRxiv - Immunology 2022Quote: ... GATA-2-expressing vector was constructed by cloning synthesized mouse GATA-2 sequences into eukaryotic expression vector pcDNA3.1 (+) (Invitrogen) with restriction enzymes of NheI and EcoRI (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... both somal and axonal compartments were incubated with 2 μM of the calcium indicator dye Fura-2-AM (Invitrogen) in imaging buffer consisting of HBSS [Ca2+ and Mg2+-free] supplemented with 10 mM HEPES (ThermoFischer) ...
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bioRxiv - Neuroscience 2021Quote: ... 8% SDS and 2% β-mercaptoethanol) were loaded onto 12% SDS-polyacrylamide gel and See-Plus 2 (Invitrogen, LC5925) was used as a molecular-weight marker ...