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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 5 μl of sample was loaded at a rate of 0.3 μl/min in 0.1% FA onto a trap column (C18, Acclaim PepMap 100 75 um x 2 cm; Thermo Fisher Scientific) and eluted across a fritless analytical resolving column (C18 ...
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bioRxiv - Neuroscience 2021Quote: 2.5 μg peptides were pre-concentrated with a flow of 3 μL/min for 10 min using a C18 trap column (Acclaim PepMap100, 100 μm x 2 cm, Thermo Scientific) and then loaded onto a 50 cm long C18 column (75 μm ID ...
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bioRxiv - Neuroscience 2020Quote: ... Each peptide fraction was separated by reverse phase chromatography on a capillary column and analysed online on a hybrid linear ion trap Orbitrap (LTQ-Orbitrap XL, Thermo Scientific) mass spectrometer for identification and relative quantification of pair peptide sequences ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...
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bioRxiv - Microbiology 2021Quote: ... trap column and separated on an Acclaim PepMap RSLC (75 μm × 50 cm, nanoViper, C18, 2 μm, 100 Å; Thermo Scientific) analytical column by increasing concentrations of 80% acetonitrile/0.1% formic acid at a flow of 250 nL/min for 90 min.
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bioRxiv - Plant Biology 2020Quote: cDNA from Venus flytrap whole-trap tissue was prepared from RNA using the Maxima First Strand cDNA Synthesis Kit (Thermo Fisher). FLYC1 cDNA (Trinity ID # comp20014_c0_seq1 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Neuroscience 2020Quote: Peptides were reconstituted in 0.1% formic acid and 2% acetonitrile and loaded onto a trap column (C18 PepMap 100 μm i.d. × 2 cm trapping column, Thermo Fisher Scientific) at 5 µL/min for 6 min using a Thermo Scientific UltiMate 3000 RSLCnano system and washed for 6 min before switching the precolumn in line with the analytical column (BEH C18 ...
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bioRxiv - Molecular Biology 2022Quote: ... Data were acquired using 120,000 resolution for the peptide measurements in the Orbitrap and a top T (3 s) method with HCD fragmentation for each precursor and fragment measurement in the ion trap following the manufacturer guidelines (Thermo Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... The nTAP-MS peptides were applied on an Acclaim PepMap 100 C18 trap column (75 μm ID x 2 cm, 3 μm, Thermo Scientific) in 0.1% formic acid ...
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bioRxiv - Neuroscience 2022Quote: 2.5 ng/2µl of resulting cDNA from TRAP library preparation was used for each qPCR reaction with 10 µl TaqMan Fast Advanced Master Mix (Applied Biosystems), 1 µl of Ahnak Prime Time qPCR primer (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... The latter was performed in the National Laboratory of Research and Services in Peptides and Proteins using an LCQ DUO ESI ion trap (Thermo Finnigan) or QExactive Orbitrap (Thermo Scientific) spectrometers.
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bioRxiv - Biochemistry 2022Quote: ... procedure.26 Peptide digest was treated with PNGase F and analysed by nano-liquid chromatography tandem mass spectrometry (nano-LC/MS/MS) on an Orbitrap Elite(tm) Hybrid Ion Trap-Orbitrap Mass Spectrometer (Thermo Scientific) using CID fragmentation ...
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bioRxiv - Cancer Biology 2022Quote: ... The samples were loaded in loading buffer (2% MeCN, 0.05% trifluoroacetic acid) and bound to an Acclaim Pepmap 100 µm × 2 cm trap (Thermo Fisher Scientific), and washed for 10 min to waste ...
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bioRxiv - Microbiology 2020Quote: ... the peptides from each gel slice in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Molecular Biology 2020Quote: ... Waters) configured with a 180 µm × 2 cm trap column coupled to a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific). A proxeon nanoelectrospray source set at 1800 V and a 75 µm (with 10 µm orifice ...
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bioRxiv - Immunology 2020Quote: ... tryptic peptides obtained from StageTip-based SCX fractionation were reconstituted in 0.1% formic acid and loaded on a nanoViper 2 cm (3 µm C18 Aq) trap column (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (50 cm ...
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bioRxiv - Immunology 2020Quote: ... Peptides were then loaded onto a Dionex UltiMate 3000 RSLCnano system via an Acclaim PepMap 100 trap column onto an Acclaim PepMap RSLC analytical column (ThermoFisher Scientific), separated using increasing concentrations of 80% ACN/0.1% FA and analyzed with a QExactive mass spectrometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... each sample was loaded on a nanoflow HPLC system fitted with a reversed-phase trap column (Acclaim PepMap100 C18, 20 mm, nanoViper, Thermo Scientific) and a heated (50 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... was loaded on a nanoflow HPLC system fitted with a reversed-phase trap column (Acclaim PepMap100 C18, 20 mm, nanoViper, Thermo Scientific) and a heated (50 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The permethylated N-glycans were dissolved in 50 µl of 1 mM sodium hydroxide in methanol/water (1:1) for infusion into a linear ion trap mass spectrometer (Orbi-LTQ;; ThermoFisher Scientific) using a nanospray source at a syringe flow rate of 0.60 µl/min and capillary temperature set to 210 °C ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide mixtures were injected in 0.1% TFA on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 min at 5 μL/min with 2% ACN ...
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Social Regulation of Egg Size Plasticity in the Honey Bee is Mediated by Cytoskeleton Organizer Rho1bioRxiv - Evolutionary Biology 2022Quote: ... Peptides were separated using a reversed-phase trap column (2 cm long, 100 μm inner diameter, filled with 5.0 μm Aqua C18 beads; Thermo Fisher Scientific) and an analytical column (15 cm long ...
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bioRxiv - Genetics 2022Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d. x 2 cm, Thermo Scientific) equilibrated with 0.05% trifluoroacetic acid in water ...
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bioRxiv - Biochemistry 2019Quote: ... The quantification of the metabolite was conducted using an API 4000 Q-Trap tandem mass spectrometry system manufactured by Applied Biosystems/MDS Sciex (Foster City ...
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bioRxiv - Biochemistry 2020Quote: ... Injected peptide samples were on-line de-salted/concentrated using an Acclaim PepMap C18 trap column (75 μm × 2 cm, Thermo Scientific) for 2 min at 5uL/min using 2% MeCN + 0.2% FA ...
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bioRxiv - Pathology 2020Quote: ... The trap column was washed for 6 minutes at the same flow rate and then the trap column was switched in-line with a resolving C18 column (Thermo Scientific) (75 μm × 50 cm ...
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bioRxiv - Genetics 2019Quote: ... and then directly coupled to an electrospray ionization source mounted on a LTQ XL linear ion trap mass spectrometer (Thermo Fisher). Chromatography was performed on an Agilent 1200 HPLC equipped with a split line to deliver a flow rate of 300 nl/min ...
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bioRxiv - Cell Biology 2019Quote: ... For each liquid chromatography coupled to tandem mass spectrometry run using an LTQ Velos linear ion trap mass spectrometer (Thermo Scientific), 4 µl were loaded onto a 18 cm x 125 µm (ID ...
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bioRxiv - Genetics 2019Quote: ... Clarified supernatant was aliquoted to a new tube and concentrated to dryness in an SC250EXP Speedvac Concentrator coupled to an RVT5105 Refrigerated Vapor Trap (Thermo Scientific). The resulting material was suspended in 0.01 mL 100% ethanol and analyzed by LC-MS as described ...
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bioRxiv - Biochemistry 2019Quote: ... The peptide samples were loaded on a nano viper trap column (C18, 5u, 100Å, 100um X 2 cm; PN: 164562, Thermo Scientific) and resolved on a 50 cm analytical column (2 um ...
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bioRxiv - Molecular Biology 2020Quote: ... coupled in line with a linear trap Quadrupole (LTQ)-Orbitrap ELITE mass spectrometer via a nano-electrospray ionization source (Thermo Scientific). Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap column (75 μm inner diameter × 2 cm ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were first loaded onto a nano trap column (Acclaim PepMap100 C18, 3 μm, 100Å, 75 μm i.d. × 2 cm, Thermo Fisher Scientific), and then separated on a reversed-phase EASY-Spray analytical column (PepMap RSLC C18 ...
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bioRxiv - Microbiology 2020Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 µm, 100 Å, 75 µm i.d. x 2 cm, Thermo Scientific) equilibrated with 0.05% TFA ...
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bioRxiv - Biochemistry 2021Quote: ... Electrospray MS was performed on the tryptic peptides using an LTQ XL linear quadrapole ion-trap mass spectrometer equipped with in-line liquid chromatography (ThermoFisher Scientific) using a C8 capillary column (300 x150 mm ...
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bioRxiv - Genetics 2019Quote: ... The resulting supernatant was transferred to a clean 4 mL glass vial and concentrated to dryness in an SC250EXP Speedvac Concentrator coupled to an RVT5105 Refrigerated Vapor Trap (Thermo Scientific). The resulting powder was suspended in 100% ethanol according to its original dry pellet weight ...
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bioRxiv - Microbiology 2021Quote: ... APCI–MS analysis was performed using an Orbitrap Elite Hybrid Ion Trap-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a standard APCI source ...
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bioRxiv - Developmental Biology 2021Quote: ... Electrospray mass spectrometry was carried out on the trypsin-digested peptides using an LTQ XL linear quadrupole ion-trap mass spectrometer equipped with in-line Accela 1250 liquid chromatography and automated sample injection (ThermoFisher Scientific). Proteome Discoverer software (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... analysis was performed using an Easy nLC 1200 UHPLC system coupled to a hybrid linear trap quadrupole Orbitrap Q-Exactive HF-X mass spectrometer (ThermoFisher Scientific). Software versions used for the data acquisition and operation were Tune 2.9 and Xcalibur 4.1.31.9 ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting supernatant was transferred to a clean 20 mL glass vial and concentrated to dryness in an SC250EXP Speedvac Concentrator coupled to an RVT5105 Refrigerated Vapor Trap (Thermo Scientific). The resulting powder was suspended in methanol and analyzed directly by HPLC-MS ...
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bioRxiv - Biochemistry 2021Quote: All liquid chromatography mass spectrometry experiments were performed on an Aglient 1200 HPLC nanoflow system coupled to a linear trap quadrupole (LTQ) Orbitrap Velos mass spectrometer (ThermoFisher Scientific). Raw data were matched to peptides and proteins using Mascot and Phenyx ...
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bioRxiv - Genetics 2020Quote: ... They were analyzed in duplicates on two separate but identical systems using linear ion trap with Orbitrap Elite analyzer and Ultimate 3000 RSLCnano System (Thermo Scientific). Protein identification was done with the UniProtKB/Swiss-Prot database [33] ...
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bioRxiv - Immunology 2021Quote: ... Tryptic peptides were separated on a nano-scale C18 HPLC capillary column and analyzed in an LTQ linear ion-trap mass spectrometer (Thermo Fisher). Peptide sequences and protein identities were assigned by matching the measured fragmentation pattern with proteins or translated nucleotide databases using Sequest ...
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bioRxiv - Immunology 2021Quote: ... Tryptic peptides were separated on a nano-scale C18 HPLC capillary column and analyzed in an LTQ linear ion-trap mass spectrometer (Thermo Fisher). Peptide sequences and protein identities were assigned by matching the measured fragmentation pattern with proteins or translated nucleotide databases using Sequest ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptide mixtures were loaded onto a PepMap 100 C18 trap column (75 μm id x 2 cm length; Thermo Fisher Scientific) at 3 μL/min for 10 min with 2 % acetonitrile (volume fraction ...
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bioRxiv - Immunology 2021Quote: ... Tryptic peptides were separated in a nano-scale C18 HPLC capillary column and analyzed in an LTQ linear ion-trap mass spectrometer (Thermo Fisher). Peptide sequences and protein identities were assigned by matching the measured fragmentation pattern with proteins or translated nucleotide databases using Sequest ...
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bioRxiv - Microbiology 2021Quote: ... fitted with a 100 μm x 2 cm PepMap100 C18 Nano Trap column and a 75 μm×25 cm reverse phase C18 nano column (Aclaim PepMap, Thermo Scientific). Samples were separated using a binary gradient consisting of buffer A (2% MeCN ...
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bioRxiv - Cell Biology 2021Quote: ... The analytical column outlet was interfaced via a nano-flow electrospray ionisation source with a linear ion trap mass spectrometer (Orbitrap Velos, Thermo Scientific). Data dependent analysis was performed using a resolution of 30,000 for the full MS spectrum ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...