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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The SARS-CoV-2 nucleocapsid gene was reverse transcribed and amplified using the TaqMan® RNA-to CT™1-Step Kit (ThermoFisher Scientific, Waltham, MA, USA), using Forward primers ...
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bioRxiv - Microbiology 2020Quote: The purified JEV structural proteins (C, M, E, ED III) from the S2 insect expression system (Invitrogen) were quantified by using the bicinchoninic acid (BCA ...
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bioRxiv - Immunology 2022Quote: ... B.1.617.2 (delta) and B.1.1.529 (Omicron)] and MERS-CoV spike using Lipofectamine 2000 (ThermoFisher Scientific cat.# 11668019) to produce single-round infection-competent pseudoviruses (80) ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit monoclonal anti-6x-His tag at 1:1,000 (Thermo Fisher Scientific, MA5-33032), and mouse monoclonal anti-β-actin at 1:10,000 (R&D Systems ...
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bioRxiv - Microbiology 2022Quote: ... or membrane probed with mouse monoclonal 6x-His tag antibody (4A12E4) (Thermofisher, Waltham, MA) (Supplementary Figure 2).
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bioRxiv - Microbiology 2019Quote: Qst was fused to a His-tag using the pEXP5-CT/TOPO vector (Invitrogen) following the TA-cloning protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: Pull-down assays were carried out using Dynabeads His-tag Isolation and Pulldown (Invitrogen). The 6His-tagged soluble pilin domains were used as bait ...
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bioRxiv - Plant Biology 2022Quote: ... and purified by Dynabeads™ His-Tag Isolation and Pulldown (ThermoFisher, Catalog number 10103D). DA1 swapped protein ubiquitylation reactions used E1 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (Thermo Fisher Scientific #MA1-21315) diluted in 5% milk in PBS-T (1:500) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Immunology 2022Quote: ... Soluble forms of the SOSV antigens with were purified by using the proteins’ 6×-His tag or StrepII tags and Expi293F cells and Expifectamine 293 expression system (Thermo Fisher Scientific) as discussed below at day 5 to 7 post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... HI Horse Serum (Invitrogen, 2% vol/vol), GlutaMax (2 mM ...
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bioRxiv - Microbiology 2023Quote: ... against the V5-tag (ThermoFisher, 46-0705, 1:1000), Flag-tag (Proteintech ...
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bioRxiv - Immunology 2022Quote: Preparation of CD5L-coated pHrodo particles: one vial containing 2 mg of particles (pHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis, ThermoFisher) were reconstituted with 500 μL of TBS with 5 mM CaCL2 and sonicated for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... All the SAM with natural isotope abundance and 2H or 13C enrichment were enzymatically synthesized from L-methionine and ATP with appropriate isotope labels. Escherichia coli (E. coli) DH5α and BL21(DE3) competent cells were from Invitrogen. Data plotting and nonlinear least-squares fitting was carried out using GraphPad Prism 7 software ...
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bioRxiv - Biochemistry 2021Quote: ... and TnsB (WP_084763316.1) proteins were codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The ShCas12k gene was inserted into the 1B (Addgene 29653 ...
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bioRxiv - Immunology 2019Quote: ... or pH sensitive fluorescent bioparticles (pHrodo E. coli Bioparticles Conjugate for Phagocytosis; Invitrogen). Each fly was injected with 27.6-32.2 nl of bioparticles (1mg/100µl ...
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bioRxiv - Biochemistry 2019Quote: ... Filtrate was added onto DNA Polymerase 1 (PolI) (E. coli source, Fisher Scientific), 0.25U/uL reaction ...
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bioRxiv - Biophysics 2024Quote: ... Ligated vectors were transformed into competent bacteria (One Shot TOP10 E. coli; Invitrogen) and grown overnight on ampicillin plates ...
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bioRxiv - Microbiology 2022Quote: ... coli Rosetta 2 (DE3)pLysS (Invitrogen) cells were grown either in Luria Bertani (LB ...
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bioRxiv - Biochemistry 2022Quote: ... was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV related coronaviruses) into 293T cells using Lipofectamine 3000 transfection reagent (L3000-001, ThermoFisher Scientific, Asheville, NC) (Naldini et al. ...
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bioRxiv - Microbiology 2020Quote: ... Drosten) and SARS-CoV (isolate HKU39849) were propagated on Vero cells in Opti-MEM I (1X) + GlutaMAX (Gibco), supplemented with penicillin (100 IU/mL ...
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bioRxiv - Microbiology 2019Quote: ... aa 1-404) monomers (rEM) and stable E dimers (rED) were expressed in the EXPI293 transient expression system (Thermo Fisher) following supplied protocols ...
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bioRxiv - Microbiology 2023Quote: ... was homogenized in 800 μL 2% FBS (Gibco, MA)/MEM (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
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bioRxiv - Immunology 2021Quote: ... The pool was incubated with biotinylated SARS CoV2 Spike extracellular domain bound to 5 μL streptavidin M280 dynabeads (ThermoFisher) for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... glycoproteins were digested with trypsin (ThermoFisher) overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and Pierce Glycoprotein Staining kit (ThermoFisher) were used for protein and glycan staining ...
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bioRxiv - Molecular Biology 2021Quote: ... we included Spike-In Mix 1 (1:1000; Life Technologies, Cat# 4456740), as from the Fluidigm manual ...
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bioRxiv - Developmental Biology 2022Quote: ... E-cadherin (rat, Life Technologies ECCD-2), Podoplanin (hamster ...
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bioRxiv - Immunology 2022Quote: ... loaded on 2% E-Gel (Thermo Fisher), and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel).
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bioRxiv - Microbiology 2019Quote: ... mouse anti-6x-His epitope tag monoclonal antibody (Thermo Fisher Scientific, Cat. No. MA1-21315); LI-COR IRDye 800CW goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Synthetic Biology 2019Quote: ... Im7-6 was purified from IVG reactions using magnetic His-tag Dynabeads (Thermo Fisher Scientific). The IVG reactions were diluted in 90 µl of Buffer 1 (50 mM NaH2PO4 and 300 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... The his-tag was cleaved using AcTEV protease according to manufacturer’s instructions (Invitrogen, Carlsbad, CA), and the his-tagged protease and noncleaved uSpike protein were removed by binding to Ni-NTA resin ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with Alexa Fluor 488–conjugated 6x-His Tag monoclonal antibody (ThermoFisher) for 1 hour at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Ni-NTA coated magnetic beads (Dynabeads™ His-Tag Isolation and Pulldown from Invitrogen, USA) were added to the solution and incubated for 10 additional min in order to bind the his-tagged TDF and PKnG proteins ...
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bioRxiv - Systems Biology 2019Quote: ... The His tag was cleaved by overnight incubation at 4 °C with SUMO protease (Invitrogen), after which the sample was loaded again onto a HisTrap FF column to recover the cleaved products ...
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bioRxiv - Developmental Biology 2020Quote: ... and verified by Western blot with primary antibodies against 6x His tag (Invitrogen, MA1-21315), and DLX1 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... the constructs were all based on a pcDNA3.1 vector containing a V5/His-tag (Invitrogen). Untagged constructs for comparison were prepared by introducing a stop codon into the pcDNA3.1/V5-His constructs using the same site-directed mutagenesis kit as above ...
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bioRxiv - Microbiology 2022Quote: ... Then 1 μl of Dynabeads™ His-Tag Isolation and Pulldown magnetic beads (ThermoFisher Scientific) were added into each well ...
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bioRxiv - Microbiology 2022Quote: ... 0.02% Tween-20) and added to 20 μl Dynabead His-Tag beads (ThermoFisher Scientific; 10103D). After incubation on a roller at 4°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... each with an N-terminal (His)6 tag from plasmids generated by GeneArt (ThermoFisher Scientific). For each purification ...
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bioRxiv - Immunology 2022Quote: ... containing a hexa-His tag was purified using HisPur Ni-NTA resin (Thermo Fisher Scientific). Expi cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Biophysics 2023Quote: ... His-tag purification was performed in Pierce disposable polypropylene 5mL disposable columns (Thermo Fisher Scientific) with a wash solution containing TNi 100/300/20 (100 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: Dynabeads™ His-Tag Isolation and Pulldown magnetic bead solution (Thermo Fisher Scientific, United States) kit was used for the detection of the binding partner of OipA ...
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bioRxiv - Cell Biology 2024Quote: ... Western blotting was performed using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) and GST monoclonal mouse antibody (own antibody) ...