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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Recombinant Mouse Pcsk9 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Biotinylated-BSA (Thermo Fisher) was diluted in ultrapure water to a concentration of 0.2 wt% and diluted in PBS to a final concentration of 0.1 wt% ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
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bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Neuroscience 2023Quote: ... The purified His-TEV-FAM19A5 proteins were digested overnight at 30°C with AcTEV protease (Invitrogen) to remove the His-tag and TEV sequence ...
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bioRxiv - Neuroscience 2024Quote: ... recombinant polyclonal goat anti-mouse coupled to Alexa Fluor 488 (Thermo Fisher, A-28175), and polyclonal goat anti-mouse coupled to Alexa Fluor 568 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Cytochalasin D (C8273, Neta) along with recombinant Mouse TNF-α (# RMTNFAI, Thermo Fisher Scientific) and human TNF-α (16769S ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated target proteins were purified with 25µL (slurry) of MyONE-C1 streptavidin beads (Thermo Fisher Scientific, 65002), pre-washed with 1x NLB + 1x PhosSTOP ...
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bioRxiv - Bioengineering 2020Quote: ... Detection of EDA positive fibronectin was performed using biotinylated fusion proteins followed by Streptavidin Alexa 488 (Invitrogen). In parallel the neovasculature marker CD31 was detected using either rat anti-mouse CD31 (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: Biotinylated cell-surface proteins were pulled down with streptavidin-coupled magnetic beads (#11205D; Thermo Fisher Scientific, US). The beads were isolated ...
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed with RIPA buffer and biotinylated proteins were precipitated with streptavidin-agarose beads (Thermo Fisher). Elution was carried out with Laemmli buffer (2% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... the biotinylated proteins were purified by incubating the lysates with High Capacity Streptavidin Agarose beads (Pierce, ThermoFisher) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated proteins were detected with streptavidin coupled to Alexa Fluor 488 or 594 (1:1000; Life Technologies).
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bioRxiv - Immunology 2022Quote: ... purified HIVJR-CSF gp120 protein was biotinylated using EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) and excess biotin was removed using a Zeba spin desalting column (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... N-terminally biotinylated 67B89 target protein was immobilized on Streptavidin Dynabeads M-270 (Invitrogen, Carlsbad, CA, USA). The target-coated beads were blocked with BSA and incubated with a suspension of 3.4·1012 phages in the presence of a 10-fold molar excess of the off-target (variant 6789) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface proteins were biotinylated with 0.5mg/ml EZ-link-Sulfo-NHS-SS-Biotin (Thermo Fisher, A39258) in a PBS buffer with 2.5mM CaCl2 and 1mM MgCl2 on ice for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and the biotinylated cell surface proteins were affinity-purified on streptavidin magnetic beads (Thermo Scientific, cat#88816). After stripping off the nonspecifically bound proteins by several rounds of washing with the lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... biotinylated protein signal was detected by incubation with streptavidin conjugated with Alexa Fluor 568 (Invitrogen, Cat# S11226) or 647 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and surface proteins were biotinylated with 0.5 mg/ml Premium Grade Sulfo-NHS-LC-Biotin (Fisher Scientific) in PBS at 4°C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse SGK1.1 (wild type and constitutively active mutant S515D) cloned into pcDNA3.1/V5-His-TOPO (Invitrogen) were kind gifts from Dr ...
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bioRxiv - Biophysics 2019Quote: ... FLAG-tagged hZP3 and HA-tagged hZP4 were synthesized (GenScript; GeneArt/Thermo Fisher Scientific) and subcloned into pHLsec329 ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were then rinsed and stained with secondary antibody (biotinylated horse anti-mouse; Gibco; BA2000; 1:2000) for 1h at room temperature before rinsing and incubation with streptavidin-HRP (SNN1004 ...
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bioRxiv - Genomics 2020Quote: ... isolated single cells were stained with mouse anti-human CD34 biotinylated antibody (clone 581, Thermo Fisher Scientific) diluted 5µL for 106 cells in FACS buffer (DPBS with 2% FBS and 1mM EDTA ...
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bioRxiv - Microbiology 2019Quote: ... Anti-NS1 antibody binding was detected by a biotinylated goat anti-mouse IgG secondary antibody solution (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... A hybridization cocktail containing the biotinylated target was incubated with the GeneChip Mouse 2.0 ST array (Affymetrix) at 60rpm for 16hr at 45°C in a Genechip Hybridisation Oven 645 ...
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bioRxiv - Biochemistry 2019Quote: ... anti V5-HRP conjugate (to detect mSCARLET and other V5 tagged fluorescent proteins, catalog number 46-0708, Invitrogen), rabbit polyclonal anti TAL1 IgG (catalog number A305-300A ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μg of GST tagged protein was conjugated to Pierce™ Glutathione Magnetic Agarose Beads (Thermo Fisher, 78602), for 1hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM maltose.) SKD3-MBP fusion protein was cleaved with a 6His-tagged AcTEV™ protease (Thermo Fisher) at 4 °C for 96 h in the amylose elution buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-tagged bait proteins were immobilized in a 96-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark) at 4°C for 18 hours (10 μg per protein in 100 μl PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... or laminin (laminin mouse protein, natural, ThermoFisher). Inhibition studies included 100 μM of Cyclo(-RGDfK ...
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bioRxiv - Cell Biology 2020Quote: ... cleared lysates were mixed with Protein G/A (50:50) recombinant protein G agarose beads (catalog #15920-010, Invitrogen, Carlsbad, CA) coupled with anti-HA (catalog #3724S ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
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bioRxiv - Cell Biology 2024Quote: SK-Mel28 cells were grown on coverslips and transiently transfected with ribonucleprotein complexes consisting of purified recombinant Cas9 protein (Truecut Cas9 Protein v2, Invitrogen, #36499) and synthetic guide RNAs (Invitrogen TrueGuide Synthetic sgRNA #35514 or Negative Control ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Immunology 2019Quote: ... The protein was expressed in Sf9 insect cells using a recombinant baculovirus system (Life Technologies). The protein was purified on a cobalt resin column and further purified by gel filtration to ensure the removal of monomeric and aggregated protein.
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bioRxiv - Biochemistry 2020Quote: ... The recombinant PfAdoMetDC protein was purified using the Strep-Tactin Sepharose column (Invitrogen, California, USA) as described previously (29 ...
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bioRxiv - Cell Biology 2022Quote: ... were combined with 0.36 μg of recombinant human protein CDK1/Cyclin B (Thermo Fisher, PV3292), 1x Kinase Buffer (Cell Signaling ...
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bioRxiv - Immunology 2021Quote: ... Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) and the purity was assessed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Cell Biology 2021Quote: Binding of recombinant proteins to pre-spotted PIP and membrane lipid strips (Thermo Fisher; P23751) was performed according to (Dowler et al. ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp ...
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bioRxiv - Immunology 2020Quote: ... 100uL of recombinant protein at 0.5 ug/ml was coated on Maxisorp™ plates (Nunc) and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... the medium was supplemented with Human Interferon-gamma Recombinant Protein (IFN-γ) (ThermoFisher, Cat. #. PHC4031) at a concentration of 50 ng/mL ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant ACE2-Fc protein which was obtained from ExpiCHO™ expression system (Thermo Fisher) was 5-fold serially diluted in DMEM medium supplementing 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... Integrin-β2 recombinant protein complexes were immobilized on Maxisorp Immuno plates (ThermoFisher, 12-565-135) and used for positive binding selections with library phage pools that were first exposed to neutravidin coated wells to deplete nonspecific binders ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Bioengineering 2023Quote: ... Hu5F9 and TJC4 were then purified using recombinant Protein A-Sepharose 4B (Thermo Fisher Scientific) buffer exchanged into PBS and concentrated using Amicon Centrifugal Filters (Millipore Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...