Labshake search
Citations for Thermo Fisher :
601 - 650 of 7736 citations for Recombinant Human BLNK His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... containing 500 μm disk patterns were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in pre-warmed DPBS (Thermo Fisher Scientific) for 3 hours at 37°C ...
-
bioRxiv - Neuroscience 2019Quote: ... IPS cells were thawed and cultured at all times cultured on recombinant human laminin LN521 (Biolamina, 2021-21) kept in E8 basal medium (Thermo Fisher Scientific) supplemented with primocin (0.1 μg/ml ...
-
bioRxiv - Developmental Biology 2021Quote: ... ESCs were dissociated with TrypLE Express for 5 mins at 37°C and induced into EpiLCs by addition of human recombinant basic fibroblast growth factor (bFGF) (Invitrogen, 13256-029) and activin A (Peprotech ...
-
bioRxiv - Cell Biology 2021Quote: ... Phages displaying human Fabs were enriched after three rounds of biopanning on biotinylated recombinant human ACE2 immobilised to streptavidin Dynabeads (Dynal M-280, Invitrogen, cat # 112.06D). After the third round of panning ...
-
bioRxiv - Microbiology 2020Quote: ... To starve Chlamydia of tryptophan HeLa cells were incubated for 24 h in media containing 2 ng/ml recombinant human IFN-γ (Invitrogen: PHC4033) prior to infection ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were pulsed in starvation buffer supplemented with 0.1% w/v bovine serum albumin at 4 °C for 40 min with 50 ng/mL human recombinant EGF (Thermo-Fisher, Gibco™, PHG0311L) to allow ligand bind to the EGFR ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.055 mM β-mercaptoethanol (#21985-023) and recombinant human full-length bFGF (#PHG0261) at 10 ng/ml (all reagents from Life Technologies).
-
bioRxiv - Microbiology 2020Quote: ... and cultured in RPMI medium supplemented with 10% FBS and differentiated for 7 days with 20 ng/mL recombinant human Macrophage-Colony Stimulating Factor protein (M-CSF) (Thermo Fisher Scientific), and 100U/L of penicillin-streptomycin solution (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
-
bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
-
bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
-
bioRxiv - Cell Biology 2024Quote: ... DD5A)29 and three controls (Ctl1B, Ctl7C, Ctl8F)47 were cultured on human recombinant laminin LN521 (Biolamina) in Essential-8TM medium (Thermo Fisher Scientific) and passaged using Trypl Express (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... Phosphorylation was induced by incubating 1 µg of recombinant human polo-like kinase 3 (PLK3) protein (Thermo Fisher Scientific, MA, USA; #PR7316B) with 50 µL of mouse WT α-synuclein in kinase reaction buffer for 8 hrs at 30°C ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and further ligated into pcDNA 3.1 V5/His TOPO vector using pcDNA 3.1 V5/His TOPO TA cloning kit (Invitrogen). The ACRD etoll was then transferred to pGADT7-AD vector using Nde1 and BamH1 sites ...
-
bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
-
bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were cloned into yeast expression vector pYES2.1/V5-His-TOPO and mammalian expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) as described previously(18).For expression studies in Drosophila S2 cells ...
-
bioRxiv - Developmental Biology 2024Quote: HEK293T cells were transferred with C-terminal HA-tagged Kayak and N-terminal GFP-tagged Jun-epi or Kayak using Lipofectamine 2000 (11668030, Thermo Fisher). The GFP-trap agarose beads (ABIN509397 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Full-length Venus-Flag-tagged CD44 and GST-tagged MSN for mammalian expression were generated using Gateway© cloning kit (Invitrogen). The vector backbones are pDEST27 vector (Invitrogen ...
-
bioRxiv - Genetics 2021Quote: ... 12 µL Hi-Di Formamide (Applied Biosystems) and 0.3 µL MapMarker 500 labelled with DY-632 (Eurogentec) ...
-
bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
-
bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
-
bioRxiv - Immunology 2021Quote: ... blot using an anti-His Ab (ThermoFisher). MSP-142 and AMA-1 were biotinylated and tetramerized with streptavidin-PE (SA-PE ...
-
bioRxiv - Microbiology 2021Quote: ... eluted in Hi-Di formamide (Applied Biosystems) with ROX-350 ladder ...
-
bioRxiv - Biochemistry 2021Quote: ... anti-His (MA1-21315, Invitrogen, 1:1,000). Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system ...
-
bioRxiv - Microbiology 2020Quote: Hi-sensitivity Qubit DNA kit (Thermo Fisher) was used to measure the DNA concentration directly from the lysate ...
-
bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% HI FBS (Gibco 10500), 20% D4T conditioned medium (24) ...
-
bioRxiv - Microbiology 2020Quote: ... and cloned into pcDNA-V5/His (Invitrogen). The M fragment of CCHFV was PCR amplified with primers shown in Table II from cDNA generated from CCHFV genomic RNA ...
-
bioRxiv - Microbiology 2021Quote: ... mouse anti-His (MA1-21315, Thermo Scientific), mouse anti-β-actin HRP (Santa Cruz Biotechnologies ...
-
bioRxiv - Biochemistry 2020Quote: ... was loaded onto His Dynabeads (Invitrogen 10103D), relying on the intrinsic metal-binding properties of untagged PrP ...
-
bioRxiv - Zoology 2019Quote: ... Primary (rabbit anti-His, Thermo Fisher Scientific) and secondary (anti-rabbit Alexa Fluor 594 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... supplemented with 10% HI FBS (Thermo Fisher). 30,000 HEK293AAV cells were added to each well of a 48 well plate one day before transfection ...
-
bioRxiv - Cell Biology 2020Quote: ... 6x-His Tag antibody is from ThermoFisher Scientific (MA1-21315-D550) ...
-
bioRxiv - Immunology 2020Quote: ... with 10 % V/V Hi FCS (Gibco), 1 mM sodium pyruvate (Gibco ...
-
bioRxiv - Biochemistry 2021Quote: ... terbium-coupled anti-his at 1nM (Invitrogen) and decreasing concentrations of compounds in 384-well microplates (Corning ...
-
bioRxiv - Biochemistry 2021Quote: ... mouse anti-His Tag (Invitrogen MA1-21315). Secondary antibody used are ...
-
bioRxiv - Neuroscience 2021Quote: ... HI Horse Serum (Invitrogen, 2% vol/vol), GlutaMax (2 mM ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 17.4 μl Hi-Di Formamide (Applied Biosystems), and 2 μL of RT reaction mixture were loaded in a 96-well plate ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-His antibody was from Thermo Fisher Scientific (37-2900 ...
-
bioRxiv - Microbiology 2022Quote: ... mouse monoclonal anti-6x-His (Thermo Fisher) diluted 1:1,000 ...
-
bioRxiv - Cell Biology 2022Quote: ... anti-His tag (ThermoFisher Scientific, MA1-21315), and anti-tubulin (DHSB ...
-
bioRxiv - Zoology 2022Quote: ... AeStaufen cDNA synthesized by Genscript was cloned into pIZT/ V5-His (Invitrogen). The same plasmid containing Chloramphenicol acetyltransferase (CAT ...
-
bioRxiv - Microbiology 2024Quote: ... 10% HI-FBS (v/v, Gibco 10082147) 100 U/mL penicillin ...
-
bioRxiv - Immunology 2024Quote: ... Trichoplusia ni (Hi-5, Thermo Fisher Scientific) and Spodoptera fugiperda (Sf9 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The antibody against His (631212, Fisher Scientific) is commercially available ...
-
bioRxiv - Neuroscience 2023Quote: The plasmid pcDNA6.1/V5-His B (Thermofisher) was used as the template to subclone in the full-length PNPLA6 cDNA (from RefSeq transcript NM_001166111.1) ...
-
bioRxiv - Microbiology 2023Quote: ... and probed with □-His mAb (Thermo Scientific) diluted 1:1,000 in 6% milk in PBS-T followed by α-mouse IgG HRP (Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: ... His Pur cobalt spin columns (Thermo Scientific) were used to purify the in vitro-translated proteins ...
-
bioRxiv - Physiology 2024Quote: A pcDNA3.1/ V5-His-TOPO plasmid (Invitrogen) containing the full-length rat Fgf23 sequence with a stabilizing mutation (R176Q ...
-
bioRxiv - Molecular Biology 2024Quote: ... for ERR1712142 or His tag antibody (ThermoFisher Cat No ...
-
bioRxiv - Microbiology 2024Quote: ... protein expression from pBAD-His/B (Invitrogen) derivative plasmids was induced at the temperature shift by adding 0.01–0.2% L-arabinose ...